Mechanisms and pathways of Toxoplasma gondii transepithelial migration

Toxoplasma gondii is a ubiquitous parasite and a prevalent food-borne parasitic pathogen. Infection of the host occurs principally through oral consumption of contaminated food and water with the gastrointestinal tract being the primary route for entry into the host. To promote infection, T. gondii has evolved highly specialized strategies for rapid traversal of the single cell thick intestinal epithelial barrier. Parasite transmigration via the paracellular pathway between adjacent cells enables parasite dissemination to secondary sites of infection where chronic infection of muscle and brain tissue is established. It has recently been proposed that parasite interactions with the integral tight junction (TJ) protein occludin influences parasite transmigration of the intestinal epithelium. We review here the emerging mechanisms of T. gondii transmigration of the small intestinal epithelium alongside the developing role played in modulating the wider TJ-associated proteome to rewire host cell regulatory systems for the benefit of the parasite

THz backward-wave oscillators for plasma diagnostic in nuclear fusion

Understanding of the anomalous transport attributed to short-scale length microturbulence through collective scattering diagnostics is key to the development of nuclear fusion energy. Signals in the subterahertz (THz) range (0.1–0.8 THz) with adequate power are required to map wider wavenumber regions. The progress of a joint international effort devoted to the design and realization of novel backward-wave oscillators at 0.346 THz and above with output power in the 1 W range is reported herein. The novel sources possess desirable characteristics to replace the bulky, high maintenance, optically pumped far-infrared lasers so far utilized in this plasma collective scattering diagnostic. The formidable fabrication challenges are described. The future availability of the THz source here reported will have a significant impact in the field of THz applications both for scientific and industrial applications, to provide the output power at THz so far not available

THz Backward-wave oscillators for plasma diagnostic in nuclear fusion

Summary form only given. The understanding of plasma turbulence in nuclear fusion is related to the availability of powerful THz sources and the possibility to map wider plasma regions. A novel approach to realize compact THz sources to be implemented in the plasma diagnostic at NSTX experiment (Princeton Plasma Physics Laboratory, USA) is reported.Two novel 0.346 THz Backward-Wave Oscillators (BWOs) have been designed and are presently in the fabrication phase. One BWO is based on the Double Staggered Grating (DSG) that supports a sheet electron beam to provide a high output power; the second BWO is based on the Double Corrugated Waveguide (DCW) that supports a cylindrical electron beam generated by a conventional Pierce gun. The performance of both the BWOs was computed by Particle-in-cells (PIC) simulations. The DSG-BWO provides about 1W of output power with a beam current of 10 mA and a beam voltage of 16.8 kV. The DCW-BWO provides 0.74W output power with 10 mA beam current and 13 kV beam voltage. The DSG and the DCW have been realized by state of the art prototype nano-CNC milling machine (DMG Mori-Seiki) that permits one to achieve performance, in term of cost and surface finishing, unavailable with any other technology. It is the first time that this technique is applied to structures above 0.3 THz. The high output power of both the BWOs demonstrates the importance of novel approaches in the emerging field of THz vacuum electron devices

Acquired and Congenital Ocular Toxoplasmosis Experimentally Induced in Calomys callosus (Rodentia, Cricetidae)

An experimental model for acquired and congenital ocular toxoplasmosis as well as a model to induce experimental autoimmune uveitis (EAU) was investigated in Calomys callosus. Toxoplasma gondii, ME-49 strain, was used to infect males and pregnant- and not pregnant-females while S-antigen, a major glycoprotein of the retinal photoreceptor cell, was used to induce EAU. The ocular lesions elicited by T. gondii were characterized by the presence of cysts, free tachyzoites and inflammatory cells in the retina or related tissues. In the congenital form, 40% of the fetus presented ocular lesions, i.e., presence of cysts in the retina, vitreous, and extra-retinal tissues. In the acquired form, 75% of the females and 50% of the males presented unilateral ocular cysts both at 21 and 47 days post-infection. It was also demonstrated that S-antigen was not uveitogenic in the C. callosus model. No lesion was observed in the animals exclusively immunized with this retinal component, even when jacalin was used as additional adjuvant for polyclonal response to the retinal antigen. It can be concluded that C. callosus may constitute in a promising model for study both acquired and congenital ocular toxoplasmosis, particularly when it is important to make sure that a non autoimmune process is involved in the genesis of the ocular infection

XIPE: the X-ray Imaging Polarimetry Explorer

X-ray polarimetry, sometimes alone, and sometimes coupled to spectral and temporal variability measurements and to imaging, allows a wealth of physical phenomena in astrophysics to be studied. X-ray polarimetry investigates the acceleration process, for example, including those typical of magnetic reconnection in solar flares, but also emission in the strong magnetic fields of neutron stars and white dwarfs. It detects scattering in asymmetric structures such as accretion disks and columns, and in the so-called molecular torus and ionization cones. In addition, it allows fundamental physics in regimes of gravity and of magnetic field intensity not accessible to experiments on the Earth to be probed. Finally, models that describe fundamental interactions (e.g. quantum gravity and the extension of the Standard Model) can be tested. We describe in this paper the X-ray Imaging Polarimetry Explorer (XIPE), proposed in June 2012 to the first ESA call for a small mission with a launch in 2017 but not selected. XIPE is composed of two out of the three existing JET-X telescopes with two Gas Pixel Detectors (GPD) filled with a He-DME mixture at their focus and two additional GPDs filled with pressurized Ar-DME facing the sun. The Minimum Detectable Polarization is 14 % at 1 mCrab in 10E5 s (2-10 keV) and 0.6 % for an X10 class flare. The Half Energy Width, measured at PANTER X-ray test facility (MPE, Germany) with JET-X optics is 24 arcsec. XIPE takes advantage of a low-earth equatorial orbit with Malindi as down-link station and of a Mission Operation Center (MOC) at INPE (Brazil).Comment: 49 pages, 14 figures, 6 tables. Paper published in Experimental Astronomy http://link.springer.com/journal/1068

Magnetic fusion energy plasma diagnostic needs novel THz BWOs

The development of collective scattering diagnostics is essential for understanding of the anomalous transport attributed to short scale length microturbulence which poses a threat to the development of nuclear fusion reactors. Signals in the sub-THz range (0.1 – 0.8 THz) with adequate power are required to probe the plasma. A joint international effort is therefore devoted to the design and realization of novel backward wave oscillators at 0.346 THz and above with output power in the 1 Watt range to replace the bulky, high maintenance optically pumped FIR lasers so far utilized for this plasma diagnostic