Alpha- and beta-adrenergic mediation of changes in metabolism and Na/K exchange in rat brown fat

Double- and triple-barreled ion-sensitive microelectrodes were used to measure changes in extracellular K+ and Na+ concentrations ([K+]o, [Na+]o) in brown fat. Redox states of different respiratory enzymes were measured simultaneously in order to correlate ion movements with metabolic activity. Trains of stimuli applied to the efferent nerves evoked two distinct increases in [K+]o. A first, small, rapid increase occurred within 10 s and accompanied a first, rapid membrane depolarization. A second, slow increase of [K+]o occurred several minutes after stimulation and accompanied a second, slow depolarization. A few seconds after stimulation onset, while the membrane was repolarizing and shifts in redox states indicated increases in lipolysis and respiration, [K+]o decreased. The [K+]o decrease was accompanied by an increase in [Na+]o, and could be partly blocked by ouabain. Phentolamine, an alpha-antagonist that blocks the first depolarization, also blocked the first, rapid [K+]o increase and part of the subsequent decrease. Propranolol, a beta-antagonist, had little effect on the first depolarization and the first increase in [K+]o, but blocked part of the subsequent [K+]o decrease and the second, slow [K+]o increase. The changes in [K+]o were almost completely abolished in the presence of both antagonists. It is concluded that brown adipocytes take up K+ and simultaneously lose Na+ in response to the interaction of noradrenaline with alpha- and beta-receptors, and this indicates a very early stimulation of the Na+ pump

A New Estimate of the Hubble Time with Improved Modeling of Gravitational Lenses

This paper examines free-form modeling of gravitational lenses using Bayesian ensembles of pixelated mass maps. The priors and algorithms from previous work are clarified and significant technical improvements are made. Lens reconstruction and Hubble Time recovery are tested using mock data from simple analytic models and recent galaxy-formation simulations. Finally, using published data, the Hubble Time is inferred through the simultaneous reconstruction of eleven time-delay lenses. The result is H_0^{-1}=13.7^{+1.8}_{-1.0} Gyr.Comment: 24 pages, 9 figures. Accepted to Ap

ExoMol molecular line lists XXXV: a rotation-vibration line list for hot ammonia

A new hot line list for $^{14}$NH$_3$ is presented. The line list CoYuTe was constructed using an accurate, empirically refined potential energy surface and a CCSD(T)/aug-cc-pVQZ ab initio dipole moment surface of ammonia, previously reported. The line list is an improvement of the ammonia line list BYTe [Yurchenko et al., Mon. Not. R. Astron. Soc., 413, 1828 (2011)]. The CoYuTe line list covers wavenumbers up to 20000 cm$^{-1}$, i.e. wavelengths beyond 0.5 $\mu$m for temperatures up to 1500 K. Comparisons with the high temperature experimental data from the literature show excellent agrement for wavenumbers below 6000 cm$^{-1}$. The CoYuTe line list contains 16.9 billion transitions and is available from the ExoMol website (www.exomol.com) and the CDS database

Extended Generalised Pareto Models for Tail Estimation

The most popular approach in extreme value statistics is the modelling of threshold exceedances using the asymptotically motivated generalised Pareto distribution. This approach involves the selection of a high threshold above which the model fits the data well. Sometimes, few observations of a measurement process might be recorded in applications and so selecting a high quantile of the sample as the threshold leads to almost no exceedances. In this paper we propose extensions of the generalised Pareto distribution that incorporate an additional shape parameter while keeping the tail behaviour unaffected. The inclusion of this parameter offers additional structure for the main body of the distribution, improves the stability of the modified scale, tail index and return level estimates to threshold choice and allows a lower threshold to be selected. We illustrate the benefits of the proposed models with a simulation study and two case studies.Comment: 18 pages, 7 figure

The Hubble time inferred from 10 time-delay lenses

We present a simultaneous analysis of 10 galaxy lenses having time-delay measurements. For each lens we derive a detailed free-form mass map, with uncertainties, and with the additional requirement of a shared value of the Hubble parameter across all the lenses. We test the prior involved in the lens reconstruction against a galaxy-formation simulation. Assuming a concordance cosmology, we obtain 1/H_0 = 13.5 (+2.5/-1.3) GyrComment: To appear in ApJ Let

Where are we? : The anatomy of the murine cortical meninges revisited for intravital imaging, immunology, and clearance of waste from the brain

Rapid progress is being made in understanding the roles of the cerebral meninges in the maintenance of normal brain function, in immune surveillance, and as a site of disease. Most basic research on the meninges and the neural brain is now done on mice, major attractions being the availability of reporter mice with fluorescent cells, and of a huge range of antibodies useful for immunocytochemistry and the characterization of isolated cells. In addition, two-photon microscopy through the unperforated calvaria allows intravital imaging of the undisturbed meninges with sub-micron resolution. The anatomy of the dorsal meninges of the mouse (and, indeed, of all mammals) differs considerably from that shown in many published diagrams: over cortical convexities, the outer layer, the dura, is usually thicker than the inner layer, the leptomeninx, and both layers are richly vascularized and innervated, and communicate with the lymphatic system. A membrane barrier separates them and, in disease, inflammation can be localized to one layer or the other, so experimentalists must be able to identify the compartment they are studying. Here, we present current knowledge of the functional anatomy of the meninges, particularly as it appears in intravital imaging, and review their role as a gateway between the brain, blood, and lymphatics, drawing on information that is scattered among works on different pathologies

Statistical models for over-dispersion in the frequency of peaks over threshold data for a flow series.

In a peaks over threshold analysis of a series of river flows, a sufficiently high threshold is used to extract the peaks of independent flood events. This paper reviews existing, and proposes new, statistical models for both the annual counts of such events and the process of event peak times. The most common existing model for the process of event times is a homogeneous Poisson process. This model is motivated by asymptotic theory. However, empirical evidence suggests that it is not the most appropriate model, since it implies that the mean and variance of the annual counts are the same, whereas the counts appear to be overdispersed, i.e., have a larger variance than mean. This paper describes how the homogeneous Poisson process can be extended to incorporate time variation in the rate at which events occur and so help to account for overdispersion in annual counts through the use of regression and mixed models. The implications of these new models on the implied probability distribution of the annual maxima are also discussed. The models are illustrated using a historical flow series from the River Thames at Kingston