53 research outputs found

    Using human-powered products for sustainability and health: benefits, challenges, and opportunities

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    This paper presents a novel research work on the potential benefits of using, a unique and sustainable energy source - the human-power. The paper discusses the benefits and the practicality of using human-power as a domestic energy source to power electrical and electronic devices and explores the benefit of its use in a more diverse perspective for possible change in behaviour and energy savings for improved sustainability in the society. The economic and sustainability gains of using Human-Powered Products (HPPs) are investigated. Two studies have been conducted, the first is a study which involves assessing the environmental impact of a human-powered products via Life Cycle Assessment (LCA); and the second is a case study called Home User Study (HUS), where the technology is experimentally tested. The human-powered system is used to explore the real benefits and life-cycle analysis towards assessing the sustainability of such products. The Home User Study(HUS)has been conducted to explore the in-situ use of human-powered systems using a new prototype that has been built and deployed. The study has used a monitoring device to measure the outcome of the interaction between the user and human-powered products. The results interestingly have revealed various other forms of benefits beyond renewable energy that could add extra motives for using human-powered products, such as health benefits. The paper also puts an emphasis on the opportunity of using Human-Powered Products which can be perceived as a great viable solution against counter measuring social issues such as physical inactivity and increasing sedentary behaviour. The results of the theoretical study indicate that Human-Powered Products (HPP) do not offer significant financial savings. The results of the HUS indicate that using human-powered products for health prospective outweigh the benefit gained from the prospective of energy savings and renewable energy. Based on the findings of this study, the paper suggests an approach that shows how the implementation of Human-powered systems could provide the opportunity to 2 sustainable energy generation and energy savings, but in all cases they can be retained through a motivational drive in improving the health benefit via encouraging physical activities

    Comparative life cycle assessment of LED lighting products

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    LED lighting products used in lighting applications and their subsequent environmental impact are growing rapidly. However, there are no in-depth updated studies that show how to assess and compare them for eco-design purposes. This research aims to add insights in this area to inform eco-design by assessing and comparing the environmental impact of a new LED eco-lighting product with an existing LED lighting product. A cradle to grave Life Cycle assessment (LCA) was conducted using ReCiPe Midpoint and Endpoint (H) life cycle impact assessment method with Simapro software. The system boundaries included all product life cycle stages, except the maintenance of the luminaires and the manufacturing of the packaging. A novel functional unit was defined for the assessment, which is more suitable for the LED lighting products. Six scenarios were considered, including three probable useful lives of the luminaires (1,000, 15,000 and 40,000 h) and two end of life options (domestic bin and recycling centre). The LCA results revealed that the new eco-lighting product has about 60% less environmental impact than the existing lighting product in all scenarios. The life cycle stages with the highest environmental impact are: 1) Use, 2) Manufacturing, 3) End of Life and 4) Transport. Based on the results obtained, recommendations for eco-design of LED lighting products were proposed, and challenges of application of LCA for the eco-design were discussed

    Sustainable product design education: current practice

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    Current production and consumption patterns are unsustainable, causing irreversible damage to the environment and human health and well-being. Designers play a vital role in resolving this problem their decisions affect product manufacturing, distribution, use, and disposal and hence they must be aware of the positive and negative impacts of their design deci-sions. Sustainable product design education is key to developing the knowl-edge, skills, and responsibility required for future generations of product designers and their educators to make informed and responsible decisions within their practice, and also enhance the social and environmental per-formance of their creations and effectively communicate the value of such decisions within a commercial context. In this article, we present insights and challenges in contemporary sustainable product design education in higher education. We document the experiences of six academics involved in teaching and researching sustainable product design in the United Kingdom, Australia, Denmark, the Netherlands, and the United States. We hope to provide a useful reference for academics seeking to adopt sustainable product design practices in their existing programs, develop new sustainable product design education programs, or reflect on their own existing product design practice

    New Abundant Microbial Groups in Aquatic Hypersaline Environments

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    We describe the microbiota of two hypersaline saltern ponds, one of intermediate salinity (19%) and a NaCl saturated crystallizer pond (37%) using pyrosequencing. The analyses of these metagenomes (nearly 784 Mb) reaffirmed the vast dominance of Haloquadratum walsbyi but also revealed novel, abundant and previously unsuspected microbial groups. We describe for the first time, a group of low GC Actinobacteria, related to freshwater Actinobacteria, abundant in low and intermediate salinities. Metagenomic assembly revealed three new abundant microbes: a low-GC euryarchaeon with the lowest GC content described for any euryarchaeon, a high-GC euryarchaeon and a gammaproteobacterium related to Alkalilimnicola and Nitrococcus. Multiple displacement amplification and sequencing of the genome from a single archaeal cell of the new low GC euryarchaeon suggest a photoheterotrophic and polysaccharide-degrading lifestyle and its relatedness to the recently described lineage of Nanohaloarchaea. These discoveries reveal the combined power of an unbiased metagenomic and single cell genomic approach

    What Stops Designers from Designing Sustainable Packaging?—A Review of Eco-design Tools with Regard to Packaging Design

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    Packaging has caused much waste and its sustainability has received much attention in the past decades. Designers have made efforts to mitigate environmental impacts of packaging. However, many packaging designs are still far from achieving their sustainability goals. The purpose of this study is to perform a literature review of the principal design methods and tools for sustainable packaging published over the last twenty years. The objective is to understand the main obstacles that limit their effective implementation in the packaging design process. This study develops a sustainable packaging design and development model and proposes criteria for accessing packaging tools and methods. This study has found that to achieve sustainable design, many tools have limitations in demonstrating usage and balancing trade-off situations. Most of the tools focus on defining problems rather than suggesting possible solutions

    Quantitative differential proteomics of yeast extracellular matrix: there is more to it than meets the eye

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    Background: Saccharomyces cerevisiae multicellular communities are sustained by a scaffolding extracellular matrix, which provides spatial organization, and nutrient and water availability, and ensures group survival. According to this tissue-like biology, the yeast extracellular matrix (yECM) is analogous to the higher Eukaryotes counterpart for its polysaccharide and proteinaceous nature. Few works focused on yeast biofilms, identifying the flocculin Flo11 and several members of the HSP70 in the extracellular space. Molecular composition of the yECM, is therefore mostly unknown. The homologue of yeast Gup1 protein in high Eukaryotes (HHATL) acts as a regulator of Hedgehog signal secretion, therefore interfering in morphogenesis and cell-cell communication through the ECM, which mediates but is also regulated by this signalling pathway. In yeast, the deletion of GUP1 was associated with a vast number of diverse phenotypes including the cellular differentiation that accompanies biofilm formation. Methods: S. cerevisiae W303-1A wt strain and gup1Δ mutant were used as previously described to generate biofilmlike mats in YPDa from which the yECM proteome was extracted. The proteome from extracellular medium from batch liquid growing cultures was used as control for yECM-only secreted proteins. Proteins were separated by SDS-PAGE and 2DE. Identification was performed by HPLC, LC-MS/MS and MALDI-TOF/TOF. The protein expression comparison between the two strains was done by DIGE, and analysed by DeCyder Extended Data Analysis that included Principal Component Analysis and Hierarchical Cluster Analysis. Results: The proteome of S. cerevisiae yECM from biofilm-like mats was purified and analysed by Nano LC-MS/MS, 2D Difference Gel Electrophoresis (DIGE), and MALDI-TOF/TOF. Two strains were compared, wild type and the mutant defective in GUP1. As controls for the identification of the yECM-only proteins, the proteome from liquid batch cultures was also identified. Proteins were grouped into distinct functional classes, mostly Metabolism, Protein Fate/Remodelling and Cell Rescue and Defence mechanisms, standing out the presence of heat shock chaperones, metalloproteinases, broad signalling cross-talkers and other putative signalling proteins. The data has been deposited to the ProteomeXchange with identifier PXD001133.Conclusions: yECM, as the mammalian counterpart, emerges as highly proteinaceous. As in higher Eukaryotes ECM, numerous proteins that could allow dynamic remodelling, and signalling events to occur in/and via yECM were identified. Importantly, large sets of enzymes encompassing full antagonistic metabolic pathways, suggest that mats develop into two metabolically distinct populations, suggesting that either extensive moonlighting or actual metabolism occurs extracellularly. The gup1Δ showed abnormally loose ECM texture. Accordingly, the correspondent differences in proteome unveiled acetic and citric acid producing enzymes as putative players in structural integrity maintenance.This work was funded by the Marie Curie Initial Training Network GLYCOPHARM (PITN-GA-2012-317297), and by national funds from FCT I.P. through the strategic funding UID/BIA/04050/2013. Fábio Faria-Oliveira was supported by a PhD scholarship (SFRH/BD/45368/2008) from FCT (Fundação para a Ciência e a Tecnologia). We thank David Caceres and Montserrat MartinezGomariz from the Unidad de Proteómica, Universidad Complutense de Madrid – Parque Científico de Madrid, Spain for excellent technical assistance in the successful implementation of all proteomics procedures including peptide identification, and Joana Tulha from the CBMA, Universidade do Minho, Portugal, for helping with the SDS-PAGE experiments, and the tedious and laborious ECM extraction procedures. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium, via the PRIDE partner repository, with the dataset identifier PXD001133. We would like to thank the PRIDE team for all the help and support during the submission process.info:eu-repo/semantics/publishedVersio

    Capturing Single Cell Genomes of Active Polysaccharide Degraders: An Unexpected Contribution of Verrucomicrobia

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    Microbial hydrolysis of polysaccharides is critical to ecosystem functioning and is of great interest in diverse biotechnological applications, such as biofuel production and bioremediation. Here we demonstrate the use of a new, efficient approach to recover genomes of active polysaccharide degraders from natural, complex microbial assemblages, using a combination of fluorescently labeled substrates, fluorescence-activated cell sorting, and single cell genomics. We employed this approach to analyze freshwater and coastal bacterioplankton for degraders of laminarin and xylan, two of the most abundant storage and structural polysaccharides in nature. Our results suggest that a few phylotypes of Verrucomicrobia make a considerable contribution to polysaccharide degradation, although they constituted only a minor fraction of the total microbial community. Genomic sequencing of five cells, representing the most predominant, polysaccharide-active Verrucomicrobia phylotype, revealed significant enrichment in genes encoding a wide spectrum of glycoside hydrolases, sulfatases, peptidases, carbohydrate lyases and esterases, confirming that these organisms were well equipped for the hydrolysis of diverse polysaccharides. Remarkably, this enrichment was on average higher than in the sequenced representatives of Bacteroidetes, which are frequently regarded as highly efficient biopolymer degraders. These findings shed light on the ecological roles of uncultured Verrucomicrobia and suggest specific taxa as promising bioprospecting targets. The employed method offers a powerful tool to rapidly identify and recover discrete genomes of active players in polysaccharide degradation, without the need for cultivation
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