Elucidating the genetic basis of antioxidant status in lettuce (Lactuca sativa).

A diet rich in phytonutrients from fruit and vegetables has been acknowledged to afford protection against a range of human diseases, but many of the most popular vegetables are low in phytonutrients. Wild relatives of crops may contain allelic variation for genes determining the concentrations of these beneficial phytonutrients, and therefore understanding the genetic basis of this variation is important for breeding efforts to enhance nutritional quality. In this study, lettuce recombinant inbred lines, generated from a cross between wild and cultivated lettuce (Lactuca serriola and Lactuca sativa, respectively), were analysed for antioxidant (AO) potential and important phytonutrients including carotenoids, chlorophyll and phenolic compounds. When grown in two environments, 96 quantitative trait loci (QTL) were identified for these nutritional traits: 4 for AO potential, 2 for carotenoid content, 3 for total chlorophyll content and 87 for individual phenolic compounds (two per compound on average). Most often, the L. serriola alleles conferred an increase in total AOs and metabolites. Candidate genes underlying these QTL were identified by BLASTn searches; in several cases, these had functions suggesting involvement in phytonutrient biosynthetic pathways. Analysis of a QTL on linkage group 3, which accounted for >30% of the variation in AO potential, revealed several candidate genes encoding multiple MYB transcription factors which regulate flavonoid biosynthesis and flavanone 3-hydroxylase, an enzyme involved in the biosynthesis of the flavonoids quercetin and kaempferol, which are known to have powerful AO activity. Follow-up quantitative RT-PCR of these candidates revealed that 5 out of 10 genes investigated were significantly differentially expressed between the wild and cultivated parents, providing further evidence of their potential involvement in determining the contrasting phenotypes. These results offer exciting opportunities to improve the nutritional content and health benefits of lettuce through marker-assisted breeding

The Higgs as a Probe of Supersymmetric Extra Sectors

We present a general method for calculating the leading contributions to h -> gg and h -> gamma gamma in models where the Higgs weakly mixes with a nearly supersymmetric extra sector. Such mixing terms can play an important role in raising the Higgs mass relative to the value expected in the MSSM. Our method applies even when the extra sector is strongly coupled, and moreover does not require a microscopic Lagrangian description. Using constraints from holomorphy we fix the leading parametric form of the contributions to these Higgs processes, including the Higgs mixing angle dependence, up to an overall coefficient. Moreover, when the Higgs is the sole source of mass for a superconformal sector, we show that even this coefficient is often calculable. For appropriate mixing angles, the contribution of the extra states to h -> gg and h -> gamma gamma can vanish. We also discuss how current experimental limits already lead to non-trivial constraints on such models. Finally, we provide examples of extra sectors which satisfy the requirements necessary to use the holomorphic approximation.Comment: v4: 34 pages, 2 figures, typo corrected and clarification adde

Moral Framing and Ideological Bias of News

News outlets are a primary source for many people to learn what is going on in the world. However, outlets with different political slants, when talking about the same news story, usually emphasize various aspects and choose their language framing differently. This framing implicitly shows their biases and also affects the reader's opinion and understanding. Therefore, understanding the framing in the news stories is fundamental for realizing what kind of view the writer is conveying with each news story. In this paper, we describe methods for characterizing moral frames in the news. We capture the frames based on the Moral Foundation Theory. This theory is a psychological concept which explains how every kind of morality and opinion can be summarized and presented with five main dimensions. We propose an unsupervised method that extracts the framing Bias and the framing Intensity without any external framing annotations provided. We validate the performance on an annotated twitter dataset and then use it to quantify the framing bias and partisanship of news

Muscle Glycogen Utilisation during an Australian Rules Football Game.

PURPOSE: To better understand the carbohydrate (CHO) requirement of Australian Football (AF) match play by quantifying muscle glycogen utilisation during an in-season AF match. METHODS: After a 24 h CHO loading protocol of 8 g/kg and 2 g/kg in the pre-match meal, two elite male forward players had biopsies sampled from m. vastus lateralis before and after participation in a South Australian Football League game. Player A (87.2kg) consumed water only during match play whereas player B (87.6kg) consumed 88 g CHO via CHO gels. External load was quantified using global positioning system technology. RESULTS: Player A completed more minutes on the ground (115 vs. 98 min) and covered greater total distance (12.2 vs. 11.2 km) than Player B, though with similar high-speed running (837 vs. 1070 m) and sprinting (135 vs. 138 m), respectively. Muscle glycogen decreased by 66% in Player A (Pre-: 656, Post-: 223 mmol∙kg-1 dw) and 24% in Player B (Pre-: 544, Post-: 416 mmol∙kg-1 dw), respectively. CONCLUSION: Pre-match CHO loading elevated muscle glycogen concentrations (i.e. >500 mmol.kg-1 dw), the magnitude of which appears sufficient to meet the metabolic demands of elite AF match play. The glycogen cost of AF match play may be greater than soccer and rugby and CHO feeding may also spare muscle glycogen use. Further studies using larger sample sizes are now required to quantify the inter-individual variability of glycogen cost of match play (including muscle and fibre-type specific responses) as well examine potential metabolic and ergogenic effects of CHO feeding

Contribution of eccentric strength to cutting performance in female soccer players

The aim of this study was to examine the contribution of eccentric strength to performance of a 70-90° cutting task (CUT) (time to complete: 5 m approach, 70-90° cut, 3 m exit). Nineteen female soccer players (mean ± SD age, height and mass; 21.6 ± 4.4 years, 1.67 ± 0.07 m and 60.5 ± 6.1 kg) from the top two tiers of English women’s soccer participated in the study. Each player performed 6 trials of the CUT task whereby three-dimensional motion data from 10 Qualisys pro-reflex cameras (240 Hz) and ground reaction forces from two AMTI force platforms (1200 Hz) were collected. Relative eccentric knee extensor (ECC-KE) and flexor peak moments (ECC-KF) were collected from both limbs at 60°·s-1 using a Kin Com isokinetic dynamometer. Hierarchical multiple regression revealed that minimum center of mass (CM) and approach velocities (CM velocity at touchdown of penultimate foot contact) could explain 82% (79% adjusted) of the variation in CUT completion time (F(1,16) = 36.086, P < 0.0001). ECC-KE was significantly (P < 0.05) moderately associated (R ≥ 0.610) with velocities at key instances during the CUT. High (upper 50th 14 percentile) ECC-KE individuals (n = 9) had significantly (P ≤ 0.01; d ≥ 1.34) greater velocities at key instances during the CUT. The findings suggest that individuals with higher ECC-KE produce faster CUT performance, by approaching with greater velocity and maintaining a higher velocity during penultimate and final contact, as they are better able to tolerate the larger loads associated with a faster approach

Renal impairment in a rural African antiretroviral programme

Background: There is little knowledge regarding the prevalence and nature of renal impairment in African populations initiating antiretroviral treatment, nor evidence to inform the most cost effective methods of screening for renal impairment. With the increasing availability of the potentially nephrotixic drug, tenofovir, such information is important for the planning of antiretroviral programmes Methods: (i) Retrospective review of the prevalence and risk factors for impaired renal function in 2189 individuals initiating antiretroviral treatment in a rural African setting between 2004 and 2007 (ii) A prospective study of 149 consecutive patients initiating antiretrovirals to assess the utility of urine analysis for the detection of impaired renal function. Severe renal and moderately impaired renal function were defined as an estimated GFR of ≤ 30 mls/min/1.73 m2 and 30–60 mls/min/1.73 m2 respectively. Logistic regression was used to determine odds ratio (OR) of significantly impaired renal function (combining severe and moderate impairment). Co-variates for analysis were age, sex and CD4 count at initiation. Results: (i) There was a low prevalence of severe renal impairment (29/2189, 1.3% 95% C.I. 0.8–1.8) whereas moderate renal impairment was more frequent (287/2189, 13.1% 95% C.I. 11.6–14.5) with many patients having advanced immunosuppression at treatment initiation (median CD4 120 cells/μl). In multivariable logistic regression age over 40 (aOR 4.65, 95% C.I. 3.54–6.1), male gender (aOR 1.89, 95% C.I. 1.39–2.56) and CD4<100 cells/ul (aOR 1.4, 95% C.I. 1.07–1.82) were associated with risk of significant renal impairment (ii) In 149 consecutive patients, urine analysis had poor sensitivity and specificity for detecting impaired renal function. Conclusion: In this rural African setting, significant renal impairment is uncommon in patients initiating antiretrovirals. Urine analysis alone may be inadequate for identification of those with impaired renal function where resources for biochemistry are limited

Cellular structure of qq-Brauer algebras

In this paper we consider the $q$-Brauer algebra over $R$ a commutative noetherian domain. We first construct a new basis for $q$-Brauer algebras, and we then prove that it is a cell basis, and thus these algebras are cellular in the sense of Graham and Lehrer. In particular, they are shown to be an iterated inflation of Hecke algebras of type $A_{n-1}.$ Moreover, when $R$ is a field of arbitrary characteristic, we determine for which parameters the $q$-Brauer algebras are quasi-heredity. So the general theory of cellular algebras and quasi-hereditary algebras applies to $q$-Brauer algebras. As a consequence, we can determine all irreducible representations of $q$-Brauer algebras by linear algebra methods

Who Are the Scrooges? Personality Predictors of Holiday Spending

The sharp increase in consumption over the holiday season has important economic implications, yet the psychology underlying this phenomenon has received limited attention. Here, we evaluate the role of individual differences in holiday spending patterns. Using 2 million transactions across 2,133 individuals, we investigate the relationship between the Big 5 personality traits on spending at Christmas. Zero-order correlations suggest holiday spending is associated with conscientiousness, neuroticism and extraversion; the relationship with neuroticism persists after accounting for possible confounders, including income and demographics. These results improve our understanding of how different personality traits predict how people respond to the environmental demands of the holiday season and have broader implications for how personality relates to consumer behavior