43 research outputs found
Robustness in Glyoxylate Bypass Regulation
The glyoxylate bypass allows Escherichia coli to grow on carbon sources with only two carbons by bypassing the loss of carbons as CO2 in the tricarboxylic acid cycle. The flux toward this bypass is regulated by the phosphorylation of the enzyme isocitrate dehydrogenase (IDH) by a bifunctional kinase–phosphatase called IDHKP. In this system, IDH activity has been found to be remarkably robust with respect to wide variations in the total IDH protein concentration. Here, we examine possible mechanisms to explain this robustness. Explanations in which IDHKP works simultaneously as a first-order kinase and as a zero-order phosphatase with a single IDH binding site are found to be inconsistent with robustness. Instead, we suggest a robust mechanism where both substrates bind the bifunctional enzyme to form a ternary complex
Loop Quantum Cosmology
Quantum gravity is expected to be necessary in order to understand situations
where classical general relativity breaks down. In particular in cosmology one
has to deal with initial singularities, i.e. the fact that the backward
evolution of a classical space-time inevitably comes to an end after a finite
amount of proper time. This presents a breakdown of the classical picture and
requires an extended theory for a meaningful description. Since small length
scales and high curvatures are involved, quantum effects must play a role. Not
only the singularity itself but also the surrounding space-time is then
modified. One particular realization is loop quantum cosmology, an application
of loop quantum gravity to homogeneous systems, which removes classical
singularities. Its implications can be studied at different levels. Main
effects are introduced into effective classical equations which allow to avoid
interpretational problems of quantum theory. They give rise to new kinds of
early universe phenomenology with applications to inflation and cyclic models.
To resolve classical singularities and to understand the structure of geometry
around them, the quantum description is necessary. Classical evolution is then
replaced by a difference equation for a wave function which allows to extend
space-time beyond classical singularities. One main question is how these
homogeneous scenarios are related to full loop quantum gravity, which can be
dealt with at the level of distributional symmetric states. Finally, the new
structure of space-time arising in loop quantum gravity and its application to
cosmology sheds new light on more general issues such as time.Comment: 104 pages, 10 figures; online version, containing 6 movies, available
at http://relativity.livingreviews.org/Articles/lrr-2005-11
Day hospital Mentalization-based treatment versus intensive outpatient Mentalization-based treatment for patients with severe borderline personality disorder: protocol of a multicentre randomized clinical trial
Forest biodiversity, ecosystem functioning and the provision of ecosystem services
Forests are critical habitats for biodiversity and they are also essential for the provision of a wide range of ecosystem services that are important to human well-being. There is increasing evidence that biodiversity contributes to forest ecosystem functioning and the provision of ecosystem services. Here we provide a review of forest ecosystem services including biomass production, habitat provisioning services, pollination, seed dispersal, resistance to wind storms, fire regulation and mitigation, pest regulation of native and invading insects, carbon sequestration, and cultural ecosystem services, in relation to forest type, structure and diversity. We also consider relationships between forest biodiversity and multifunctionality, and trade-offs among ecosystem services. We compare the concepts of ecosystem processes, functions and services to clarify their definitions. Our review of published studies indicates a lack of empirical studies that establish quantitative and causal relationships between forest biodiversity and many important ecosystem services. The literature is highly skewed; studies on provisioning of nutrition and energy, and on cultural services, delivered by mixed-species forests are under-represented. Planted forests offer ample opportunity for optimising their composition and diversity because replanting after harvesting is a recurring process. Planting mixed-species forests should be given more consideration as they are likely to provide a wider range of ecosystem services within the forest and for adjacent land uses. This review also serves as the introduction to this special issue of Biodiversity and Conservation on various aspects of forest biodiversity and ecosystem services
Does overexpression of HER-2 correlate with clinicopathological characteristics and prognosis in colorectal cancer? Evidence from a meta-analysis
Surgery and risk for multiple sclerosis: a systematic review and meta-analysis of case–control studies
Characterizing multistationarity regimes in biochemical reaction networks
12 páginas, 5 figuras, 3 tablasSwitch like responses appear as common strategies in the regulation of cellular systems. Here we present a method to characterize bistable regimes in biochemical reaction networks that can be of use to both direct and reverse engineering of biological switches. In the design of a synthetic biological switch, it is important to study the capability for bistability of the underlying biochemical network structure. Chemical Reaction Network Theory (CRNT) may help at this level to decide whether a given network has the capacity for multiple positive equilibria, based on their structural properties. However, in order to build a working switch, we also need to ensure that the bistability property is robust, by studying the conditions leading to the existence of two different steady states. In the reverse engineering of biological switches, knowledge collected about the bistable regimes of the underlying potential model structures can contribute at the model identification stage to a drastic reduction of the feasible region in the parameter space of search. In this work, we make use and extend previous results of the CRNT, aiming not only to discriminate whether a biochemical reaction network can exhibit multiple steady states, but also to determine the regions within the whole space of parameters capable of producing multistationarity. To that purpose we present and justify a condition on the parameters of biochemical networks for the appearance of multistationarity, and propose an efficient and reliable computational method to check its satisfaction through the parameter space.Funding provided by Spanish government, Ministry of Science and Innovation project MultiSysBio (ref. DPI2008-06880-C03-02) and DPI2004-07444-C04-03, Xunta de Galicia (PGIDIT02-PXIC40209PN). Consejo Superior de Investigaciones CientÃÂficas intramural project BioREDES (ref. PIE-201170E018). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewe