Desórdenes de la locomoción, a dos velocidades de paso, en pacientes con fibromialgia

El objetivo del presente estudio es analizar si hay diferencias en las variables espaciotemporales de locomoción entre las pacientes afectadas con fibromialgia (FM) y un grupo de mujeres sanas, analizadas a velocidad confortable y rápida. Se evaluaron a 55 mujeres con FM y 44 sanas. El análisis de la locomoción se realizó con una plataforma de presiones (GaitRite system) y los sujetos realizaron una prueba a velocidad confortable y otra a velocidad rápida. Diferencias significativas (p<0.001) fueron encontradas entre el grupo control y el grupo FM en las variables de velocidad, longitud de paso, cadencia, fase de apoyo monopodal, fase de apoyo bipodal, fase de oscilación y fase de apoyo en las dos condiciones analizadas. No encontramos diferencias significativas entre las pacientes con FM caminando rápido y el grupo control a velocidad confortable, excepto en la cadencia. Los parámetros de locomoción en mujeres con FM están severamente afectados al compararlo con el grupo control, debido a diversos factores como la bradicinesia, la falta de actividad física, el sobrepeso, la fatiga y el dolor, que junto con una menor fuerza isométrica de las extremidades inferiores, pueden ser las responsables de estas alteraciones en la locomoción y en el descenso de la calidad de vida de estas pacientes.The aim of the present study was to determine if there are differences in spatiotemporal parameters among patients affected by fibromyalgia (FM) and healthy subjects, assess at comfortable and quickly speed of locomotion. We studied 55 women with FM and 44 controls. Gait analysis was performed using an instrumented walkway for measurement of the kinematic parameters of gait (GAITRite system), and the subjects was assessment in two conditions, comfortable and fast gait speed. Significant differences (p<0.001) between FM and control groups were found in velocity, stride length, cadence, single support ratio, double support ratio, stance phase ratio, and swing phase ratio at both analysis conditions. There aren’t statistical differences between FM group walking at quick speed and control group walking at comfortable speed, except in cadence variable. Gait parameters of women affected by FM were severely impaired when compared to those of healthy women. Different factors such as lack of physical activity, bradikinesia, overweight, fatigue, and pain together with a lower isometric force in the legs can be responsible for the alterations in gait and poorer life quality of women with FM.Peer Reviewe

A functional BH3 domain in an aquaporin from Leishmania infantum.

Despite the absence of sequences showing significant similarity to any of the members of the Bcl-2 family of proteins in protozoa, experiments carried out in yeast or trypanosomatids have demonstrated that ectopic expression of some of these members alters their response to different death stimuli. Because the BH3 domain is the smallest common signature in all the proteins of this family of apoptosis regulators and also because they are essential for molecular interactions between antagonistic members, we looked for sequences with significant similarity to the BH3 motif in the Leishmania infantum genome. Among the top scoring ones, we found the MYLALQNLGDEV amino-acid stretch at the C terminus of a previously described aquaporin, now renamed as Li-BH3AQP. This motif is highly conserved in homologous proteins from other species of the Leishmania genus. The association of Li-BH3AQP with human Bcl-XL was demonstrated by both co-immunoprecipitation and yeast two-hybrid experiments. Ectopic expression of Li-BH3AQP reduced viability of HeLa cells and this deleterious effect was abrogated by the simultaneous overexpression of Bcl-XL. Although we were not able to demonstrate a reduction in parasite viability when the protein was overexpressed in Leishmania promastigotes, a prodeath effect could be observed when the parasites overexpressing Li-BH3AQP were treated with staurosporine or antimycin A. Surprisingly, these parasites were more resistant, compared with wild-type parasites, to hypotonic stress or nutrient deprivation. The prodeath activity was abolished upon replacement of two highly conserved amino acids in this BH3 domain. Taken together, these results point to Li-BH3AQP as the first non-enzymatic protein ever described in trypanosomatids that is involved in cell death

Diagnóstico de producción en ganado lechero en pastoreo de un grupo ganadero de validación y transferencia de tecnología

The regionalization of framing issues for producers Livestock Group of Validation and Technology Transfer (GGAVATT) from San Jose Miahuatlán Veracruz, Mexico, under the current production of native grass prairie with mainly during the months of higher temperature have a poor performance and thus milk production declines which promotes a great use of concentrated feeds which causes an increase in production cost and less use to them. For this purpose we made a diagnosis of current conditions GGAVATT production, yielding information that helps us source and compared to determine precisely what the effect of applying the proposed alternative. We propose an alternative implementation of pastures with improved pastures needed to produce a greater amount of forage per unit area especially in the colder months.La regionalización de la problemática encuadrando a los productores del Grupo Ganadero de Validación y Transferencia de Tecnología (GGAVATT) de San José Miahuatlán Veracruz, México, bajo la actual situación de producción de praderas básicamente con gramas nativas que durante los meses de menor frío tienen un pobre desempeño y por ende la producción de leche se deteriora lo cual promueve una gran utilización de alimentos concentrados lo que provoca un incremento en el costo de producción y una menor utilidad para estos. Para dicho propósito se realizó un diagnóstico de las condiciones actuales de producción del GGAVATT, arrojando información que nos sirve de partida y de comparación para determinar con precisión cuál es el efecto de aplicar la alternativa propuesta. Se plantea como alternativa la implantación de praderas con pastos mejorados que permitan producir una mayor cantidad de forrajes por unidad de superficie sobre todo en los meses de menor temperatura

Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant

Availability of immature embryos as explants to establish wheat (Triticum aestivum L.) by tissue culture can be limited by climatic factors and the lack of high quality embryos frequently hampers experimentation. This study evaluates the effects of rainfall, various temperature-based variables and sunshine duration on tissue culture response (TCR) traits including callus formation (CF), regenerating calli (RC), and number of plants per embryo (PPE) for 96 wheat genotypes of worldwide origin. The objectives of this study were to evaluate the significance of a particular climatic factor on TCR traits and to determine the period of wheat growth during which these factors were the most effective. The genotypes were grown in an experimental field during three seasons differing in meteorological conditions. The relationships between TCR traits and climatic factors within three time periods of wheat growth: 2, 6 and 10 weeks prior to embryo sampling were analysed by biplot analysis. The tissue culture traits were influenced at very different degrees by climatic factors: from 16.8% (RC) to 69.8% (CF). Donor plant environment with high temperatures and low rainfalls reduced (p lt 0.05) the tissue culture performance of wheat genotypes. Callus formation was most sensitive to the temperature based factors. The environmental conditions between flowering and the medium milk stage were the most important for CF, while RC and PPE were not particularly related to any period

Málaga: Desde Málaga a playa del Ejido (Málaga) Serie B: Gestión.

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Cabo de Gata: Promontorio de cabo de Gata (Almería)

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Almerimar: Aguadulce a Balanegra (Almería). Serie B: Gestión

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San José: Desde Las Negras a Cabo de Gata (Almería). Serie B: Gestión.

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Almería: Desde cabo de Gata a Aguadulce (Almería). Serie C: Modelos y Geomorfología

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