Leaf stomata conductance, leaf water potential and soil water status in Panicum maximum jacq. in disturbed and non‐disturbed microsites in a semi‐arid ecosystem in kenya

Tropical semi-arid ecosystems are intricate ecosystems characterized byalternating dry-wet cycles. The question of how trees and grasses coexist under a considerable range of environmental and management conditions has been referred to as the "savanna problem" The practical significance of understanding the dynamics of natural ecosystems in relation to  disturbances induced by herbivory, changing land use patterns or climate change is increasingly being recognized. Natural resource conservationists, range managers, and other custodians of natural resources require concrete information bases for land use policy formulations and as a means of regulating land use systems for sustainable resource use and development. An experiment using a randomised complete block design was set up to measure the effects of induced disturbances on ecosystem components and concomitant plant physiological responses. Analysis ofvariance techniques were used to determine the presence or absence of significant treatment effects. Disturbed micro]sites (manipulated treatments where Acacia tortilis trees were removed) demonstrated contrasting results compared to the non-disturbed micro-sites (Acacia tortilis trees left intact).The disturbed sites had less average moisture content in the 10cm soil profile (14.4%) than the nondisturbed sites(18.8%). Panicum maximum had an average stomata conductanceof a magnitude of 0.65cms-1(270mmolm-2 s-1) for the disturbed microsites and 0.75cms-1(312mmolm-2s-1) for the non-disturbed sites. There were no significant treatment effects in transpiration rates and leaf water potential of Panicum maximum in the disturbed and non-disturbed sites. This analysis demonstrates that long term changes in  microenvironmental conditions of soil and plant water status due to tree removal is likely to cause shifts in botanical composition of graminoid species with direct implications on nitrogen sequestration, species biodiversity, and productivity.Key words: Panicum maximum, semi-arid ecosystems, soil water status, stomata conductance, tree-grass interactions, micro-environmental fluxe

LHR effects in nonducted whistler propagation ? new observations and numerical modelling

International audienceVLF-ELF broadband measurements onboard the MAGION 4 and 5 satellites at heights above 1 Re in plasmasphere provide new data on various known phenomena related to ducted and nonducted whistler wave propagation. Two examples are discussed: magnetospherically reflected (MR) whistlers and lower hybrid resonance (LHR) noise band. We present examples of rather complicated MR whistler spectrograms not reported previously and argue the conditions for their generation. Analytical consideration, together with numerical modelling, yield understanding of the main features of those spectrograms. LHR noise band, as well as MR whistlers, is a phenomenon whose source is the energy propagating in the nonducted way. At the plasmaspheric heights, where hydrogen (H+) is the prevailing ion, and electron plasma frequency is much larger than gyrofrequency, the LHR frequency is close to its maximumvalue in a given magnetic field. This frequency is well followed by the observed noise bands. The lower cutoff frequency of this band is somewhat below that maximum value. The reason for this, as well as the possibility of using the LHR noise bands for locating the plasma through position, are discussed

Sexually dimorphic role for vasopressin in the development of social play

Despite the well-established role of vasopressin (AVP) in adult social behavior, its role in social development is relatively unexplored. In this paper, we focus on the most prominent social behavior of juvenile rats, social play. Previous pharmacological experiments in our laboratory suggested that AVP regulates play in a sex- and brain region-specific manner in juvenile rats. Here we investigate the role of specific AVP systems in the emergence of social play. We first characterize the development of play in male and female Wistar rats and then ask whether the development of AVP mRNA expression correlates with the emergence of play. Unexpectedly, play emerged more rapidly in weanling-aged females than in males, resulting in a sex difference opposite of that typically reported for older, juvenile rats. AVP mRNA and play were correlated in males only, with a negative correlation in the bed nucleus of the stria terminalis and a positive correlation in the paraventricular nucleus of the hypothalamus. These findings support the hypothesis that AVP acts differentially on multiple systems in a sex-specific manner to regulate social play and suggest a role for PVN and BNST AVP systems in the development of play. Differential neuropeptide regulation of male and female social development may underlie well-documented sex differences in incidence, progression, and symptom severity of behavioral disorders during development

Growth of quantum dot coated core-shell anisotropic nanowires for improved thermal and electronic transport

Anisotropic nanowires are promising candidates for electronic thermal management due to their unique electrical and thermal properties.However, eco-friendly solution-processed nanomaterials with an elaborate morphology and microstructure for modulating thermal andcharge transfer are still a considerable challenge. Herein, we present a simple but effective approach for synthesizing pseudo core-shell nano-wires through quantum dot (QD)-like nanostructure coating (p-NW@QD) to generate exceptional electron-phonon transport properties.With the assistance of diphenyl ether as a coordination solvent, high crystallinity lead sulfide NWs can be fabricated with a large aspect ratiotogether with uniform QD coating. Thisp-NW@QD exhibits high electronic mobility (30.65 cm2/Vs) as well as a diameter independent lowthermal conductivity (1.5361 W/m K). Direct charge/heat carrier flow measurements and computational simulations demonstrate that theunusual electrical and thermal transport phenomenon is strongly dependent on the fast charge transport through the QD shell, and a slowphonon migration across the Umklapp process dominated NW cores. These findings indicate a significant step toward colloidal synthesisnanostructures for future high-performance nanoelectronics and thermal energy devices

Interpreting 16S metagenomic data without clustering to achieve sub-OTU resolution

The standard approach to analyzing 16S tag sequence data, which relies on clustering reads by sequence similarity into Operational Taxonomic Units (OTUs), underexploits the accuracy of modern sequencing technology. We present a clustering-free approach to multi-sample Illumina datasets that can identify independent bacterial subpopulations regardless of the similarity of their 16S tag sequences. Using published data from a longitudinal time-series study of human tongue microbiota, we are able to resolve within standard 97% similarity OTUs up to 20 distinct subpopulations, all ecologically distinct but with 16S tags differing by as little as 1 nucleotide (99.2% similarity). A comparative analysis of oral communities of two cohabiting individuals reveals that most such subpopulations are shared between the two communities at 100% sequence identity, and that dynamical similarity between subpopulations in one host is strongly predictive of dynamical similarity between the same subpopulations in the other host. Our method can also be applied to samples collected in cross-sectional studies and can be used with the 454 sequencing platform. We discuss how the sub-OTU resolution of our approach can provide new insight into factors shaping community assembly.Comment: Updated to match the published version. 12 pages, 5 figures + supplement. Significantly revised for clarity, references added, results not change

Metabolic flexibility as a major predictor of spatial distribution in microbial communities

A better understand the ecology of microbes and their role in the global ecosystem could be achieved if traditional ecological theories can be applied to microbes. In ecology organisms are defined as specialists or generalists according to the breadth of their niche. Spatial distribution is often used as a proxy measure of niche breadth; generalists have broad niches and a wide spatial distribution and specialists a narrow niche and spatial distribution. Previous studies suggest that microbial distribution patterns are contrary to this idea; a microbial generalist genus (Desulfobulbus) has a limited spatial distribution while a specialist genus (Methanosaeta) has a cosmopolitan distribution. Therefore, we hypothesise that this counter-intuitive distribution within generalist and specialist microbial genera is a common microbial characteristic. Using molecular fingerprinting the distribution of four microbial genera, two generalists, Desulfobulbus and the methanogenic archaea Methanosarcina, and two specialists, Methanosaeta and the sulfate-reducing bacteria Desulfobacter were analysed in sediment samples from along a UK estuary. Detected genotypes of both generalist genera showed a distinct spatial distribution, significantly correlated with geographic distance between sites. Genotypes of both specialist genera showed no significant differential spatial distribution. These data support the hypothesis that the spatial distribution of specialist and generalist microbes does not match that seen with specialist and generalist large organisms. It may be that generalist microbes, while having a wider potential niche, are constrained, possibly by intrageneric competition, to exploit only a small part of that potential niche while specialists, with far fewer constraints to their niche, are more capable of filling their potential niche more effectively, perhaps by avoiding intrageneric competition. We suggest that these counter-intuitive distribution patterns may be a common feature of microbes in general and represent a distinct microbial principle in ecology, which is a real challenge if we are to develop a truly inclusive ecology

Genomic Expansion of Magnetotactic Bacteria Reveals an Early Common Origin of Magnetotaxis with Lineage-specific Evolution

The origin and evolution of magnetoreception, which in diverse prokaryotes and protozoa is known as magnetotaxis and enables these microorganisms to detect Earth’s magnetic field for orientation and navigation, is not well understood in evolutionary biology. The only known prokaryotes capable of sensing the geomagnetic field are magnetotactic bacteria (MTB), motile microorganisms that biomineralize intracellular, membrane-bounded magnetic single-domain crystals of either magnetite (Fe3O4) or greigite (Fe3S4) called magnetosomes. Magnetosomes are responsible for magnetotaxis in MTB. Here we report the first large-scale metagenomic survey of MTB from both northern and southern hemispheres combined with 28 genomes from uncultivated MTB. These genomes expand greatly the coverage of MTB in the Proteobacteria, Nitrospirae, and Omnitrophica phyla, and provide the first genomic evidence of MTB belonging to the Zetaproteobacteria and “Candidatus Lambdaproteobacteria” classes. The gene content and organization of magnetosome gene clusters, which are physically grouped genes that encode proteins for magnetosome biosynthesis and organization, are more conserved within phylogenetically similar groups than between different taxonomic lineages. Moreover, the phylogenies of core magnetosome proteins form monophyletic clades. Together, these results suggest a common ancient origin of iron-based (Fe3O4 and Fe3S4) magnetotaxis in the domain Bacteria that underwent lineage-specific evolution, shedding new light on the origin and evolution of biomineralization and magnetotaxis, and expanding significantly the phylogenomic representation of MTB