Tryptic phosphopeptides from whole casein. I. Preparation and analysis by fast protein liquid chromatography

Tryptic phosphopeptides were obtained from whole bovine casein by chromatography on the anion exchange resin QAE-Sephadex A 25. Salt gradient elution of the column allowed separation of non-phosphorylated peptides from phosphorylated species. The preparations obtained contained at least seven distinct phosphopeptides of which the following casein fragments were identified: αs1(43-58):2P, αs1(59-79): 5P, αs2(46-70): 4P, β(1-28): 4P, β(2-28): 4P, and β(33-48): 1P. Fast protein liquid chromatography (FPLC) on Mono Q HR 5/5 resin showed that the phosphopeptides were eluted in the same order as from the QAE-Sephadex resin. However, on the analytical column HR 5/5 the fragments αs1(59-79): 5P and β(2-28): 4P, having the same net charge under the conditions of chromatography, co-eluted, whereas they were at least partly separated on the preparative column HR 16/10. Following enzymic dephosphorylation, the peptides eluted at lower salt strength in the gradient. FPLC on Mono Q resin thus permitted dephosphorylation to be monitored and intermediates between the parent species and the fully dephosphorylated peptide to be identifie

Prediction of laminar/turbulent transition in an unstructured finite element Navier Stokes solver using a boundary layer code with emphasis on cross flow transition

International audienceThis paper presents some developments performed to achieve an accurate and efficient prediction tool for laminar/turbulent transition. These developments involve industrial codes used at Dassault Aviation for the aerodynamics design of military aircraft and business jets. The process is based on a flexible sequence of simulation tools. The issues concerning influence of the numerical solvers, transition onset criteria and the coupling process are described. The influence of intermittency function is also discussed. Results demonstrate the accuracy of the method with emphasis on transition induced by crossflow instabilities

À propos de la question d’histoire contemporaine du programme d’agrégation 2006 et 2007 « Les campagnes dans les évolutions sociales et politiques de l’Europe de 1830 à la fin des années 1920 : étude comparée (France, Allemagne, Espagne, Italie) »

L’histoire rurale, par le biais d’une question de concours, va se trouver pendant deux années sur le devant de la scène scientifique et éditoriale. De même que la revue Histoire et sociétés rurales, dans sa livraison du 1er semestre 2005, a proposé une première lecture et une bibliographie raisonnée de la nouvelle question d’histoire contemporaine pour l’agrégation d’histoire et le CAPES d’histoire-géographie , et alors que d’autres équipes, nationales ou européennes, s’apprêtent à publier su..

À propos de la question d’histoire contemporaine du programme d’agrégation 2006 et 2007 « Les campagnes dans les évolutions sociales et politiques de l’Europe de 1830 à la fin des années 1920 : étude comparée (France, Allemagne, Espagne, Italie) »

L’histoire rurale, par le biais d’une question de concours, va se trouver pendant deux années sur le devant de la scène scientifique et éditoriale. De même que la revue Histoire et sociétés rurales, dans sa livraison du 1er semestre 2005, a proposé une première lecture et une bibliographie raisonnée de la nouvelle question d’histoire contemporaine pour l’agrégation d’histoire et le CAPES d’histoire-géographie , et alors que d’autres équipes, nationales ou européennes, s’apprêtent à publier su..

From Metamodeling to Automatic Generation of Multimodal Interfaces for Ambient Computing

International audiencehis paper presents our approach to design multichannel and multimodal applications as part of ambient intelligence. Computers are increasingly present in our environments, whether at work (computers, photocopiers), at home (video player, hi-fi, microwave), in our cars, etc. They are more adaptable and context-sensitive (e.g., the car radio that lowers the volume when the mobile phone rings). Unfortunately, while they should provide smart services by combining their skills, they are not yet designed to communicate together. Our results, mainly based on the use of a software bus and a workflow, show that different devices (such as Wiimote, multi-touch screen, telephone, etc.) can be coordinated in order to activate real things (such as lamp, fan, robot, webcam, etc.). A smart digital home case study illustrates how using our approach to design with ease some parts of the ambient system and to redesign them during runtime

Stress-Induced Sphingolipid Signaling: Role of Type-2 Neutral Sphingomyelinase in Murine Cell Apoptosis and Proliferation

International audienceBACKGROUND: Sphingomyelin hydrolysis in response to stress-inducing agents, and subsequent ceramide generation, are implicated in various cellular responses, including apoptosis, inflammation and proliferation, depending on the nature of the different acidic or neutral sphingomyelinases. This study was carried out to investigate whether the neutral Mg(2+)-dependent neutral sphingomyelinase-2 (nSMase2) plays a role in the cellular signaling evoked by TNFalpha and oxidized LDLs, two stress-inducing agents, which are mitogenic at low concentrations and proapoptotic at higher concentrations. METHODOLOGY AND PRINCIPAL FINDINGS: For this purpose, we used nSMase2-deficient cells from homozygous fro/fro (fragilitas ossium) mice and nSMase2-deficient cells reconstituted with a V5-tagged nSMase2. We report that the genetic defect of nSMase2 (in fibroblasts from fro/fro mice) does not alter the TNFalpha and oxidized LDLs-mediated apoptotic response. Likewise, the hepatic toxicity of TNFalpha is similar in wild type and fro mice, thus is independent of nSMase2 activation. In contrast, the mitogenic response elicited by low concentrations of TNFalpha and oxidized LDLs (but not fetal calf serum) requires nSMase2 activation. CONCLUSION AND SIGNIFICANCE: nSMase2 activation is not involved in apoptosis mediated by TNFalpha and oxidized LDLs in murine fibroblasts, and in the hepatotoxicity of TNFalpha in mice, but is required for the mitogenic response to stress-inducing agents

073: Very long-term effects of pacing therapy in Hypertrophic Obstructive Cardiomyopathy (HOCM)

The clinical value of DDD pacing as primary treatment of HOCM remains controversial. Very long-term data are lacking.Aimssingle-centre observational study aimed at describing the very long term effects on symptoms, clinical and echocardiographic outcomesPatients54 patients (59±14 years) with symptomatic (NYHA Class >2) drug-refractory HOCM implanted with a DDD pacemaker with or without defibrillator between 1991 and 2007 and followed up to 20 years (mean 11.5; range 0,4-21,8).Main resultsare summarised in table. No patient had myomectomy or septal ablation during follow-up (f/u). NYHA functional class and other symptoms were significantly improved at 1-2 years and at the end of f/u. Left ventricular outflow tract (LVOT) gradient decreased by a mean of 78% at 1-2 years and 89% at end f/u consistent with SAM resolution. LV ejection fraction decreased over time with a mean value of 56% at end f/u without evidence of cavity dilatation. The actuarial survival rate was 90% at 5-yrs and 65% at 10-yrs. 24 patients died, 19 from non cardiac cause and 5 cardiovascular. 2 patients had heart transplant after 8 and 13yrs.ConclusionThe clinical and echocardiographic outcome of HOCM patients treated by DDD pacing seems favourable, inviting to re-evaluate the exact value of the therapy in further controlled studiesTable – Main results.Baseline3 months1-2 yearsEnd f/uP valueNYHA functional class, (%)<0,0001Grade 10313536Grade 243535957Grade 3521667Grade 45000Syncope/nearsyncope (%)76/482/22/22/2<0,0001Angina (%)57444<0,0001LVOT gradient (mmHg)79±3620±2411±158±21<0,0001SAM (%)96383016<0,0001LVEF (%)63,5±7,561±759±756±90,05LVEDD (%)47±5NANA43±120,3

Impacto de la erupción del volcán Huaynaputina del año 1600 d.C. en pueblos aledaños

El volcán Huaynaputina, en el sur de Perú, registró la erupción histórica más grande (VEI 6) en los Andes en el año 1600 d.C, la misma que sucedió durante la transición entre el Imperio Inca y el Virreinato del Perú. Este evento tuvo graves con secuencias en los Andes Centrales y un impacto climático global. Crónicas españolas informaron que existían al menos 15 aldeas o asentamientos alrededor del volcán, de los cuales siete de ellos fueron totalmente destruidos por la erupción. Los estudios multidisciplinarios que hemos implementado en los últimos años nos han permitido identificar y analizar las características de seis pueblos enterrados por la erupción. La caída de tefra y las corrientes de densidad piroclástica (PDCs), tuvieron impactos diferentes según la distancia del pueblo al cráter, la ubicación con respecto al emplazamiento de las PDCs a lo largo de los valles, las características geomorfológicas del sitio, y el tipo de construcciones

The Huayruro Project: mapping the Calicanto Inca area buried by the A.D. 1600 Huaynaputina eruption, with geophysical imaging and remote sensing

We present geophysical and remote sensing observations near the Quinistaquillas town (southern Peru), in the framework of the HUAYRURO Project. This Inca zone was buried during the A.D. 1600 Huaynaputina eruption, the most important volcanic phenomenon of the last 400 years. The eruption had a global impact, due to the volume of emitted ash (2-3 times the one emitted by Vesuvius in A.D. 79). This lead to a 1.13°C cooling of the planet and caused a worlwide agricultural crisis. During the eruption, the Calicanto-Chimpapampa zone was covered by ashes and pyroclastic flows, with a thickness in the range [1 - 20] m. From 2015 to 2017, remote sensing and geophysical methods were deployed to map a ~ 1 km*2 km area, up to 3-m depth