Boosting Long-term Memory via Wakeful Rest: Intentional Rehearsal is not Necessary, Automatic Consolidation is Sufficient.

<div><p>People perform better on tests of delayed free recall if learning is followed immediately by a short wakeful rest than by a short period of sensory stimulation. Animal and human work suggests that wakeful resting provides optimal conditions for the consolidation of recently acquired memories. However, an alternative account cannot be ruled out, namely that wakeful resting provides optimal conditions for intentional rehearsal of recently acquired memories, thus driving superior memory. Here we utilised non-recallable words to examine whether wakeful rest boosts long-term memory, even when new memories could not be rehearsed intentionally during the wakeful rest delay. The probing of non-recallable words requires a recognition paradigm. Therefore, we first established, via Experiment 1, that the rest-induced boost in memory observed via free recall can be replicated in a recognition paradigm, using concrete nouns. In Experiment 2, participants heard 30 non-recallable non-words, presented as ‘foreign names in a bridge club abroad’ and then either rested wakefully or played a visual spot-the-difference game for 10 minutes. Retention was probed via recognition at two time points, 15 minutes and 7 days after presentation. As in Experiment 1, wakeful rest boosted recognition significantly, and this boost was maintained for at least 7 days. Our results indicate that the enhancement of memory via wakeful rest is <i>not</i> dependent upon intentional rehearsal of learned material during the rest period. We thus conclude that consolidation is <i>sufficient</i> for this rest-induced memory boost to emerge. We propose that wakeful resting allows for superior memory consolidation, resulting in stronger and/or more veridical representations of experienced events which can be detected via tests of free recall and recognition.</p></div

Impact and detectability of hypothetical CCS offshore seep scenarios as an aid to storage assurance and risk assessment

Carbon Capture and Storage has the potential to make a significant contribution to the mitigation of climate change, however there is a regulatory and societal obligation to demonstrate storage robustness and minimal local environmental impact. This requires an understanding of environmental impact potential and detectability of a range of hypothetical leak scenarios. In the absence of a significant body of real-world release experiments this study collates the results of 86 modelled scenarios of offshore marine releases derived from five different model systems. This synthesis demonstrates a consistent generalised relationship between leak rate, detectability and impact potential of a wide range of hypothetical releases from CO2 storage, which can be described by a power law. For example a leak of the order of 1 T per day should be detectable at, at least, 60 m distance with an environmental impact restricted to less than a 15 m radius of the release point. Small releases are likely to require bottom mounted (lander) monitoring to ensure detection. In summary this work, when coupled with a quantification of leakage risk can deliver a first order environmental impact assessment as an aid to the consenting process. Further this work demonstrates that non-catastrophic release events can be detected at thresholds well below levels which would undermine storage performance or significantly impact the environment, given an appropriate monitoring strategy

Impact potential of hypersaline brines released into the marine environment for CCS reservoir pressure management

The environmental impact potential arising from the possible disposal of hypersaline brines into the ocean as part of reservoir pressure management for Carbon Capture and Storage is assessed using sophisticated high-resolution hydrodynamic models for the first time, investigating several industry guided scenarios. Although the characteristics of some brines in their undiluted form would have a high environmental impact potential, we find that dispersion in a hydrodynamically active region like the North Sea acts to dilute disposed brine rapidly, even in a worst case approach, such that the potential impact footprint (area exposed to environmentally damaging salinity or temperature) is small, measured in 10’s of meters depending on the release scenario and site specific data such as the hypersaline water contaminants along with in-situ conditions such as currents and mixing. The method of brine disposal has a significant influence on dispersal, such that brines released nearer the sea surface disperse more rapidly, compared with release at the seabed. Hence consideration of brine release height is recommended to further limit impact potentia

Optimising environmental monitoring for carbon dioxide sequestered offshore

Carbon Capture and Storage (CCS) provides a mechanism by which CO2 can be removed from the atmosphere and stored in reservoirs. Regulations and stakeholder assurance require monitoring to show storage is robust. The marine environment is heterogeneous and dynamic, and baselines are extremely variable. Hence, distinguishing anomalous CO2 from natural variability is challenging. Monitoring schemes must be designed to identify releases early and with certainty, whilst being cost effective. A key question is how to deploy the smallest number of sensors to ensure effective monitoring? We approached this problem through a 3D hydrodynamic model (FVCOM) coupled to a carbonate system. The unstructured grid resolution ranges from 0.5 km to 3 m and simulates seabed release scenarios ranging from 3 t d−1 to 300 t d−1 using the Goldeneye complex as an exemplar test bed. This configuration allows us to characterise and analyse the fate of CO2 in the water column, with the spatial and temporal CO2 patterns shown to be affected by both tides and seasonal mixing/stratification. A weighted greedy set algorithm is used to identify the positions within the model domain which yield the greatest combined coverage for the smallest number of sampling stations, further limited by selecting only a feasible number of sample sites. The weighted greedy set algorithm incorporates the effect of the variable grid spacing as well as the proximity of the sample locations to the Goldeneye complex. The weighted greedy set can identify releases sooner, with a stronger signal than a regular sampling approach

The effects of aromatase inhibitors on lipids and thrombosis

Oestrogen is known to influence blood lipid levels and though its cardioprotective effects are less clear than once thought, there remains concern that reduction of oestrogen levels during hormonal treatment for breast cancer may have an adverse effect on cardiovascular risk. While tamoxifen has been shown to improve lipid profiles, the aromatase inhibitors have a very different mode of action and do not possess the oestrogen-agonistic effects of tamoxifen. At present, there are few data on the effects of these agents on lipid profiles. Available data are mixed, but suggest that the different aromatase inhibitors have different effects on lipid profiles. Some studies show anastrozole as generally having little effect on lipids, while others have indicated adverse effects on lipid profiles/increased hypercholesterolaemia. Letrozole has been associated with adverse effects on lipid profiles in some studies, including BIG 1-98, but short-term data from randomised trials do not show increased cardiovascular morbidity. By contrast, exemestane, which has been studied in slightly more detail, may either have little effect or may be associated with slightly improved lipid profiles. In general, the changes have been small and are likely to be of little relevance in women with advanced breast cancer, but if these agents come to be used in early breast cancer, their impact on lipid profiles may become more important. Many studies are currently underway with the aromatase inhibitors, with safety assessments including monitoring lipid levels. The results of these studies are keenly awaited

Patient's needs and preferences in routine follow-up after treatment for breast cancer

The purpose of the study was to analyse the needs of women who participated in a routine follow-up programme after treatment for primary breast cancer. A cross-sectional survey was conducted using a postal questionnaire among women without any sign of relapse during the routine follow-up period. The questionnaire was sent 2-4 years after primary surgical treatment. Most important to patients was information on long-term effects of treatment and prognosis, discussion of prevention of breast cancer and hereditary factors and changes in the untreated breast. Patients preferred additional investigations (such as X-ray and blood tests) to be part of routine follow-up visits. Less satisfaction with interpersonal aspects and higher scores on the Hospital Anxiety and Depression Scale (HADS) scale were related to stronger preferences for additional investigation. Receiving adjuvant hormonal or radiotherapy was related to a preference for a more intensive follow-up schedule. There were no significant differences between patients treated with mastectomy compared to treated with breast-conserving therapy. During routine follow-up after a diagnosis of breast cancer, not all patients needed all types of information. When introducing alternative follow-up schedules, individual patients' information needs and preferences should be identified early and incorporated into the follow-up routine care, to target resources and maximise the likelihood that positive patient outcomes will result

Dissecting Inflammatory Complications in Critically Injured Patients by Within-Patient Gene Expression Changes: A Longitudinal Clinical Genomics Study

By studying gene expression changes over time in a cohort of trauma patients, Keyur Desai and colleagues identify genes and pathways strongly associated with longer-term complications, which could lead to improved outcome prediction in the first 80 hours after injury

ADRB2 Arg16Gly Polymorphism, Lung Function, and Mortality: Results from the Atherosclerosis Risk in Communities Study

BACKGROUND: Growing evidence suggests that the Arg16Arg genotype of the beta-2 adrenergic receptor gene may be associated with adverse effects of beta-agonist therapy. We sought to examine the association of beta-agonist use and the Arg16Gly polymorphism with lung function and mortality among participants in the Atherosclerosis Risk in Communities study. METHODOLOGY AND PRINCIPAL FINDINGS: We genotyped study participants and analyzed the association of the Arg16Gly polymorphism and beta-agonist use with lung function at baseline and clinical examination three years later and with all-cause mortality during 10 years of follow-up. Lung function was characterized by percent-predicted forced expiratory volume in 1 second. Associations were examined separately for blacks and whites. Black beta-agonist users with the Arg/Arg genotype had better lung function at baseline and at the second clinical visit than those with Arg/Gly and Gly/Gly genotypes. Adjusted mean percent-predicted FEV(1) was 21% higher in Arg/Arg subjects compared to Gly/Gly at baseline (p = 0.01) and 20% higher than Gly/Gly at visit 2 (p = 0.01). Arg/Gly subjects had adjusted percent-predicted FEV(1) 17% lower than Arg/Arg at baseline but were similar to Arg/Arg subjects at visit 2. Although black beta-agonist users with the Arg/Arg genotype appeared to have better crude survival rates, the association between genotype and all-cause mortality was inconclusive. We found no difference in lung function or mortality by genotype among blacks who did not use beta-agonists or among whites, regardless of beta-agonist use. CONCLUSIONS: Black beta-agonist users with the ADRB2 Arg16Arg genotype had better lung function, and, possibly, better overall survival compared to black beta-agonist users with the Gly16Gly genotype. Our findings highlight the need for additional studies of sufficient size and statistical power to allow examination of outcomes among beta-agonist users of different races and genotypes

Asthma families show transmission disequilibrium of gene variants in the vitamin D metabolism and signalling pathway

The vitamin D prophylaxis of rickets in pregnant women and newborns may play a role in early allergic sensitization. We now asked if an already diseased population may have inherited genetic variants in the vitamin D turnover or signalling pathway. Serum levels of calcidiol (25-OH-D(3)) and calcitriol (1,25-(OH)(2)-D(3)) were retrospectively assessed in 872 partipants of the German Asthma Family Study. 96 DNA single base variants in 13 different genes were genotyped with MALDI-TOF and a bead array system. At least one positive SNP with a TDT of p < 0.05 for asthma or total IgE and calcidiol or calcitriol was seen in IL10, GC, IL12B, CYP2R1, IL4R, and CYP24A1. Consistent strong genotypic association could not be observed. Haplotype association were found only for CYP24A1, the main calcidiol degrading enzyme, where a frequent 5-point-haplotype was associated with asthma (p = 0,00063), total IgE (p = 0,0014), calcidiol (p = 0,0043) and calcitriol (p = 0,0046). Genetic analysis of biological pathways seem to be a promising approach where this may be a first entry point into effects of a polygenic inherited vitamin D sensitivity that may affect also other metabolic, immunological and cancerous diseases