DNA-induced spatial entrapment of general transcription machinery can stabilize gene expression in a nondividing cell.

Funder: Wellcome TrustAn important characteristic of cell differentiation is its stability. Only rarely do cells or their stem cell progenitors change their differentiation pathway. If they do, it is often accompanied by a malfunction such as cancer. A mechanistic understanding of the stability of differentiated states would allow better prospects of alleviating the malfunctioning. However, such complete information is yet elusive. Earlier experiments performed in Xenopus oocytes to address this question suggest that a cell may maintain its gene expression by prolonged binding of cell type-specific transcription factors. Here, using DNA competition experiments, we show that the stability of gene expression in a nondividing cell could be caused by the local entrapment of part of the general transcription machinery in transcriptionally active regions. Strikingly, we found that transcriptionally active and silent forms of the same DNA template can stably coexist within the same nucleus. Both DNA templates are associated with the gene-specific transcription factor Ascl1, the core factor TBP2, and the polymerase II (Pol-II) ser5 C-terminal domain (CTD) phosphorylated form, while Pol-II ser2 CTD phosphorylation is restricted to the transcriptionally dominant template. We discover that the active and silent DNA forms are physically separated in the oocyte nucleus through partition into liquid-liquid phase-separated condensates. Altogether, our study proposes a mechanism of transcriptional regulation involving a spatial entrapment of general transcription machinery components to stabilize the active form of a gene in a nondividing cell

Illusions and Cloaks for Surface Waves

Open access articleEver since the inception of Transformation Optics (TO), new and exciting ideas have been proposed in the field of electromagnetics and the theory has been modified to work in such fields as acoustics and thermodynamics. The most well-known application of this theory is to cloaking, but another equally intriguing application of TO is the idea of an illusion device. Here, we propose a general method to transform electromagnetic waves between two arbitrary surfaces. This allows a flat surface to reproduce the scattering behaviour of a curved surface and vice versa, thereby giving rise to perfect optical illusion and cloaking devices, respectively. The performance of the proposed devices is simulated using thin effective media with engineered material properties. The scattering of the curved surface is shown to be reproduced by its flat analogue (for illusions) and vice versa for cloaks.Engineering and Physical Sciences Research Council (EPSRC

Cymatics for the cloaking of flexural vibrations in a structured plate

Based on rigorous theoretical findings, we present a proof-of-concept design for a structured square cloak enclosing a void in an elastic lattice. We implement high-precision fabrication and experimental testing of an elastic invisibility cloak for flexural waves in a mechanical lattice. This is accompanied by verifications and numerical modelling performed through finite element simulations. The primary advantage of our square lattice cloak, over other designs, is the straightforward implementation and the ease of construction. The elastic lattice cloak, implemented experimentally, shows high efficiency

Comparação entre métodos de determinação da biomassa microbiana baseados no princípio da fumigação e extração.

We report measurement of the cross section of $e^+e^-\to \pi^+\pi^-\psi(2S)$ between 4.0 and $5.5 {\rm GeV}$, based on an analysis of initial state radiation events in a $980 \rm fb^{-1}$ data sample recorded with the Belle detector. The properties of the $Y(4360)$ and $Y(4660)$ states are determined. Fitting the mass spectrum of $\pi^+\pi^-\psi(2S)$ with two coherent Breit-Wigner functions, we find two solutions with identical mass and width but different couplings to electron-positron pairs: $M_{Y(4360)} = (4347\pm 6\pm 3) {\rm MeV}/c^2$, $\Gamma_{Y(4360)} = (103\pm 9\pm 5) {\rm MeV}$, $M_{Y(4660)} = (4652\pm10\pm 8) {\rm MeV}/c^2$, $\Gamma_{Y(4660)} = (68\pm 11\pm 1) \rm MeV$; and ${\cal{B}}[Y(4360)\to \pi^+\pi^-\psi(2S)]\cdot \Gamma_{Y(4360)}^{e^+e^-} = (10.9\pm 0.6\pm 0.7) \rm eV$ and ${\cal{B}}[Y(4660)\to \pi^+\pi^-\psi(2S)]\cdot \Gamma_{Y(4660)}^{e^+e^-} = (8.1\pm 1.1\pm 0.5) \rm eV$ for one solution; or ${\cal{B}}[Y(4360)\to \pi^+\pi^-\psi(2S)]\cdot \Gamma_{Y(4360)}^{e^+e^-} = (9.2\pm 0.6\pm 0.6) \rm eV$ and ${\cal{B}}[Y(4660)\to \pi^+\pi^-\psi(2S)]\cdot \Gamma_{Y(4660)}^{e^+e^-} = (2.0\pm 0.3\pm 0.2) \rm eV$ for the other. Here, the first errors are statistical and the second systematic. Evidence for a charged charmoniumlike structure at $4.05 {\rm GeV}/c^2$ is observed in the $\pi^{\pm}\psi(2S)$ intermediate state in the $Y(4360)$ decays.Comment: 21 pages, 12 figure

Water and ion pairing in polyelectrolyte multilayers

The water content in multilayers made from poly(styrenesulfonate) and poly(diallyldimethylammonium chloride) was determined using infrared spectroscopy and thermal gravimetric analysis. Under ambient conditions the polyelectrolyte multilayer contains 10-20 wt % water, which is removed by heating under dry conditions to 100 °C. FTIR comparison of multilayers with solution-precipitated polyelectrolyte complexes of the same polymers revealed that both the composition and sulfonate group environment were identical