9 research outputs found
Quantificação de macro e micro nutrientes em algumas etnovariedades de cubiu (Solanum sessiliflorum Dunal)
Influência do envelhecimento acelerado na anatomia da testa de sementes de soja, cv. Monsoy 8400
Pervasive gaps in Amazonian ecological research
Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4
While the increasing availability of global databases on ecological communities has advanced our knowledge
of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In
the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of
Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus
crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced
environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian
Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by
2050. This means that unless we take immediate action, we will not be able to establish their current status,
much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio
Endothelial Barrier Integrity Is Disrupted In Vitro by Heme and by Serum From Sickle Cell Disease Patients
Free extracellular heme has been shown to activate several compartments of innate immunity, acting as a danger-associated molecular pattern (DAMP) in hemolytic diseases. Although localized endothelial barrier (EB) disruption is an important part of inflammation that allows circulating leukocytes to reach inflamed tissues, non-localized/deregulated disruption of the EB can lead to widespread microvascular hyperpermeability and secondary tissue damage. In mouse models of sickle cell disease (SCD), EB disruption has been associated with the development of a form of acute lung injury that closely resembles acute chest syndrome (ACS), and that can be elicited by acute heme infusion. Here we explored the effect of heme on EB integrity using human endothelial cell monolayers, in experimental conditions that include elements that more closely resemble in vivo conditions. EB integrity was assessed by electric cell-substrate impedance sensing in the presence of varying concentrations of heme and sera from SCD patients or healthy volunteers. Heme caused a dose-dependent decrease of the electrical resistance of cell monolayers, consistent with EB disruption, which was confirmed by staining of junction protein VE-cadherin. In addition, sera from SCD patients, but not from healthy volunteers, were also capable to induce EB disruption. Interestingly, these effects were not associated with total heme levels in serum. However, when heme was added to sera from SCD patients, but not from healthy volunteers, EB disruption could be elicited, and this effect was associated with hemopexin serum levels. Together our in vitro studies provide additional support to the concept of heme as a DAMP in hemolytic conditions.Sao Paulo Research Foundation (FAPESP), grants 2016/14172-6 to EP, and 2014/00984-3 to FFC, respectively; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) Brazil; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior – Brasil (CAPES), finance code 001;
and FAEPEX-UNICAM
Determinação de elementos essenciais e não essenciais em palmito de pupunheira Determination of essential and non-essential elements in palm heart of peach palm
Considerando-se a atual situação epidemiológica do Brasil em que a obesidade e as doenças crônico-degenerativas (doenças cardiovasculares, diabetes mellitus e câncer) passam a ser destaque em saúde pública, parece prudente a preocupação quanto à caracterização química dos alimentos regionais com potencial econômico e nutricional, em especial os de baixo teor calórico, como o palmito. Sendo assim, determinou-se a composição centesimal, fitato e elementos minerais (macro e micro) no palmito de pupunheira in natura e cozido. O palmito de pupunheira utilizado foi o da raça Pampa Hermosa, da Estação Experimental de Fruticultura da Coordenação de Pesquisas em Ciências Agronômicas (CPCA) do Instituto Nacional de Pesquisas da Amazônia (INPA), em Manaus (AM), coletado no período chuvoso, em janeiro de 1995. Tomou-se aleatoriamente 3,5 kg de palmito de 110 progênies de pupunha inerme, com 2 anos de idade, por ocasião do primeiro corte. As palmeiras foram cultivadas em Latossolo Amarelo, isento de adubação. As análises químicas foram efetuadas no Laboratório de Nutrição e Físico-Química dos Alimentos do INPA e IPEN-CNEN. De acordo com os resultados obtidos, verificou-se que o palmito, in natura e cozido, apresentou em 100g da parte comestível respectivamente, alto teor de umidade, em torno de 90%; 1,5 g de proteínas; baixo teor de lipídio (0,3 e 0,2 g) e; concentrações importantes de fibra alimentar (3,8 e 2,2 g). Em relação aos elementos minerais nutricionalmente importantes sobressairam, também em 100g da parte comestível, K (194,0 mg; 128,0 mg); Ca (42,4 mg ; 42,9 mg); Mg (3,4 mg; 2,2 mg); Fe (237,7 µg; 265,2 µg), Se (2,1 µg; 2,8 µg), respectivamente no palmito in natura e cozido. Dos elementos minerais não essenciais na alimentação, destacaram-se o bromo (830,4 ±5,8 µg%; 434±5,8 µg%) e o rubídio (315,2±3,2 µg%; 433,5±34,0 µg%) respectivamente para palmito in natura e cozido. A concentração de fitato (hexafosfato de mioinositol) foi de 554,7 mg% para o palmito in natura e 379,1 mg%, para o palmito cozido. Apesar do palmito ser um alimento dietético sob o ponto de vista nutricional, devido à baixa concentração em calorias e à presença de fibra, o mesmo pode contribuir no aporte de minerais essenciais na alimentação da população Amazônica.<br>In recent years, there has been a steady increase in the ocurrence of obesity and chronic or degenerative diseases (cardiovascular, diabetes mellitus, cancer) in Brazil. Therefore, studies on the nutritional composition of regional foodstuffs, especially those with low calorific content and high commercial value, are highly justified. The aim of this study was to evaluate the centesimal phytate and mineral micro or macroelement composition of in natura or cooked palm heart of peach palm (Pampa Hermosa race), collected in January 1995 (rainy season) in the fruit experimental station of the Coordenação de Pesquisas em Ciências Agronômicas (CPCA), Instituto Nacional de Pesquisas da Amazônia (INPA), in Manaus, Brazil. Chemical analyses were conducted on a total of 3.5 kg of plant heart samples, taken at random from 110 spineless progeny from Yurimáguas, Peru, which were grown on non-fertilized soil. Analyses were conducted in the Nutrition and Food Physico-Chemical laboratory of INPA and IPEN-CNEN. Each 100 g of the edible part of in natura or cooked palm heart provided low concentrations of lipids (0.3 and 0.2 g, respectively) and high concentrations of fibre (3.8 and 2.2, respectively). Both type of palm heart contained (in 100 g): 90% humidity and 1.5 g protein. The results for nutritionally important minerals were as follows (in 100 g of in natura or cooked palm heart, respectively): K (194.0 mg; 128.0 mg), Ca (42.4 mg; 42.9 mg), Mg (3.4 mg; 2.2 mg), Fe (237.7 µg; 265.2 µg), and Se (2.1 µg: 2.8 µg). The concentrations of non essential elements minerals in in natura and cooked palm heart were, respectively, as follows: bromine (830.4±5.8 µg%; 434.0±5.8 µg%) and rubidium (315.2±3.2 µg%; 433.5±34.0 µg%). The concentration of phytate in 100 g of palm heart, was as follows: 554.7 mg (in natura) 379.1 mg (cooked). Although palm heart can be considered a dietetic foodstuff, being rich in fibre and low in calories, it can be recommended as a good source of minerals for the Amazonian populations
Immunocompromised patients with acute respiratory distress syndrome : Secondary analysis of the LUNG SAFE database
The aim of this study was to describe data on epidemiology, ventilatory management, and outcome of acute respiratory distress syndrome (ARDS) in immunocompromised patients. Methods: We performed a post hoc analysis on the cohort of immunocompromised patients enrolled in the Large Observational Study to Understand the Global Impact of Severe Acute Respiratory Failure (LUNG SAFE) study. The LUNG SAFE study was an international, prospective study including hypoxemic patients in 459 ICUs from 50 countries across 5 continents. Results: Of 2813 patients with ARDS, 584 (20.8%) were immunocompromised, 38.9% of whom had an unspecified cause. Pneumonia, nonpulmonary sepsis, and noncardiogenic shock were their most common risk factors for ARDS. Hospital mortality was higher in immunocompromised than in immunocompetent patients (52.4% vs 36.2%; p < 0.0001), despite similar severity of ARDS. Decisions regarding limiting life-sustaining measures were significantly more frequent in immunocompromised patients (27.1% vs 18.6%; p < 0.0001). Use of noninvasive ventilation (NIV) as first-line treatment was higher in immunocompromised patients (20.9% vs 15.9%; p = 0.0048), and immunodeficiency remained independently associated with the use of NIV after adjustment for confounders. Forty-eight percent of the patients treated with NIV were intubated, and their mortality was not different from that of the patients invasively ventilated ab initio. Conclusions: Immunosuppression is frequent in patients with ARDS, and infections are the main risk factors for ARDS in these immunocompromised patients. Their management differs from that of immunocompetent patients, particularly the greater use of NIV as first-line ventilation strategy. Compared with immunocompetent subjects, they have higher mortality regardless of ARDS severity as well as a higher frequency of limitation of life-sustaining measures. Nonetheless, nearly half of these patients survive to hospital discharge. Trial registration: ClinicalTrials.gov, NCT02010073. Registered on 12 December 2013