Impact of Daily Thermocycles on Hatching Rhythms, Larval Performance and Sex Differentiation of Zebrafish

In the wild, water temperature cycles daily: it warms up after sunrise, and cools rapidly after sunset. Surprisingly, the impact of such daily thermocycles during the early development of fish remains neglected. We investigated the influence of constant vs daily thermocycles in zebrafish, from embryo development to sexual differentiation, by applying four temperature regimens: two constant (24°C and 28°C) and two daily thermocycles: 28:24°C, TC (thermophase coinciding with daytime, and cryophase coinciding with night-time) and 24:28°C, CT (opposite to TC) in a 12:12 h light:dark cycle (LD). Embryo development was temperature-dependent but enhanced at 28°C and TC. Hatching rhythms were diurnal (around 4 h after lights on), but temperature- and cycle-sensitive, since hatching occurred sooner at 28°C (48 hours post fertilization; hpf) while it was delayed at 24°C (96 hpf). Under TC, hatching occurred at 72 hpf, while under CT hatching displayed two peaks (at 70 hpf and 94 hpf). In constant light (LL) or darkness (DD), hatching rhythms persisted with tau close to 24 h, suggesting a clock-controlled "gating" mechanism. Under 28°C or TC, larvae showed the best performance (high growth and survival, and low malformations). The sex ratio was strongly influenced by temperature, as the proportion of females was higher in CT and TC (79 and 83% respectively), contrasting with 28°C and 24°C, which led to more males (83 and 76%). Ovarian aromatase (cyp19a) expression in females was highest in TC and CT (6.5 and 4.6 fold higher than at 28°C, respectively); while anti-müllerian hormone (amh) expression in males increased in testis at 24°C (3.6 fold higher compared to TC) and particularly at 28°C (14.3 fold increase). Taken together, these findings highlight the key role of environmental cycles during early development, which shaped the daily rhythms in fish embryo and larvae, and ultimately influenced sex differentiation

Numerical methods for the computation of the confluent and Gauss hypergeometric functions

The two most commonly used hypergeometric functions are the confluent hypergeometric function and the Gauss hypergeometric function. We review the available techniques for accurate, fast, and reliable computation of these two hypergeometric functions in different parameter and variable regimes. The methods that we investigate include Taylor and asymptotic series computations, Gauss-Jacobi quadrature, numerical solution of differential equations, recurrence relations, and others. We discuss the results of numerical experiments used to determine the best methods, in practice, for each parameter and variable regime considered. We provide 'roadmaps' with our recommendation for which methods should be used in each situation

Ultra-high pressure LC determination of glucosamine in shrimp by-products and migration tests of chitosan films

Chitosan, a multiple applications molecule, was isolated from shrimp by-products by fermentation. The amount of chitosan in the solid fraction of the fermented extract was measured after its conversion in the respective glucosamine units. The procedure includes an acid hydrolysis (110 ºC, 4 h with HCl 8 M) and a derivatization with 9-fluorenylmethyl chloroformate (Fmoc-Cl). Ultra-high-pressure liquid chromatography method was developed and optimized. Excellent peaks resolution was achieved in just 10 min. The method was evaluated in what concerns to validation parameters such as linearity, repeatability, quantification limit, and recovery. Migration tests of films prepared with chitosan were carried out in two simulants: ultrapure water and ethanol 95% (v/v).FONCICYT (Fund for International Cooperation of Science and Technology EU-Mexico

Managing the global heritage city of Mexico City:adapting the HUL approach to the globalised urban context

This chapter explores the complexities of adapting the HUL approach to the context of global heritage cities through the analysis of Mexico City, assessing its existing urban heritage planning and management systems and identifying effective policies and tools for the implementation of the toolkit addressed in the Historic Urban Landscape (HUL) Recommendation. The assessment of this case leads to the identification of complexities and analysis of best tools adopted by different stakeholders to cope with the management challenges. In parallel with the HUL toolkit, these specific instruments and strategies include the adoption of an integral management plan that conducts vulnerability assessment and strengthens the capacities of relevant parties to execute programmes, the establishment of innovative partnership models and grassroots initiatives as an effective community engagement tool that brings together voluntary groups, experts and local administrative bodies, along with the assignment of a site management unit and a local peripheral body to serve as a catalyst and enhance better communication among stakeholders. Focusing specifically on Mexico City, this chapter intends to make an original contribution to literature by introducing the size, scope and complexities of global cities to the management of historic urban landscape discourse. Furthermore, it defines a new perspective for the adaptation of the HUL approach to the globalised urban context and tests the feasibility and effectiveness of the tools developed in the Recommendation