Influence of low-tannin sorghum on performance and bone morphometrics of male Ross 308 broilers aged 1 - 42 days

The objective of this study was to determine the effects of low tannin sorghum as a maize replacement on the performance and bone morphometrics of Ross 308 broiler chickens. A total of 250 one-day-old broiler chickens were allotted to a complete randomized design with five treatments replicated five times. Birds were offered varying sorghum levels as maize replacement at 0% (control), 25%, 50%, 75% and 100%, formulated to be isonutritive and isoenergetic. The condensed tannin and total phenolic contents of the sorghum were analysed. Bodyweight and feed intake were measured weekly to calculate feed conversion ratio (FCR), and mortality was recorded as it occurred. Digestibility measurements were done when the chickens were between 15 and 21 days and between 35 and 42 days old. At ages 21 and 42 days, two chickens per pen were slaughtered to determine bone characteristics. A digital calliper was used to measure the length and diameter of the bones, and an electronic scale was used to determine the weight of the bones. Ash, calcium and phosphorus concentrations of the bones were determined. The Seedor and robusticity indices of the tibia were also calculated. The general linear model procedure of Statistical Analysis Software was used to analyse the data. At 1 - 21 days old bodyweight was higher for birds fed a level of 50% sorghum than those that were offered the control diet. Replacing maize with sorghum improved the metabolizable energy (ME) of broiler chickens aged 42 days. Bodyweight and FCR of birds fed diets with 50%, 75% and 100% sorghum were higher and better, respectively, than those on diets with 25% and 0% sorghum at 22 - 42 days old. Bone morphometries of chickens aged 1 - 21 days and 22 - 42 days were not affected by replacing maize with sorghum. Thus, maize can be replaced by a low tannin white sorghum without causing adverse effects on chickens.Keywords: minerals, tibia bone, Seedor and robusticity indice

Blood profiles of indigenous Pedi goats fed varying levels of Vachellia karroo leaf meal in Setaria verticillata hay-based diet

Vachellia karroo (Acacia karroo) is promising fodder for goats in the critical dry season in communal rangelands. The only limitation to the use of this fodder tree is the presence of phenolic compounds, such as condensed tannins. A study was conducted to investigate the effects of tanniniferous V. karroo leaf meal feeding on blood profiles of indigenous Pedi goats fed a basal diet of Setaria verticillata grass hay. Twenty indigenous Pedi goats, weighing 18 ± 2 kg, were allocated in a completely randomized design to five dietary treatments containing V. karroo leaf meal at 20% (S80A20), 25% (S75A25), 30% (S70A30), 40% (S60A40) and 50% (S50A50) of the total diet in a 22-day trial. Twelve ml of blood were collected from the jugular vein from each goat before and after the experiment for haematological and serum biochemical assays. Daily dry matter intake (DMI) was similar across treatments, ranging from 633 g to 765 g per goat per day. There was no difference in initial and final bodyweights of goats consuming various experimental diets. However, bodyweight gains were significantly higher in goats fed a diet with 50% leaf meal as compared with other treatment groups. There were no differences in haematological indices of Pedi goats except for mean corpuscular haemoglobin (Hb) concentration. Goats fed 50% leaf meal had significantly lower mean corpuscular haemoglobin concentration (MCHC) values as compared with other treatment groups. Similarly, there were no differences in the blood serum chemistry of goats that consumed various inclusion levels of V. karroo, except for serum total protein (TP) and alanine aminotransferase (ALT). Goats fed 50% leaf meal had depressed serum TP, while serum enzyme Alanine aminotransferase (ALT) concentration decreased significantly in goats fed 25% leaf meal as compared with those on the 20% dietary treatment. Tannin concentration of 8.2 g/kg dry matter (DM) had no toxic effect on experimental animals. A 40 % inclusion of V. karroo in a Setaria verticillata hay-based diet may be fed to indigenous Pedi goats without compromising the immunity system and health of the animals.Keywords: Condensed tannin, fodder, haematology, seru

Supermassive Binaries and Extragalactic Jets

Some quasars show Doppler shifted broad emission line peaks. I give new statistics of the occurrence of these peaks and show that, while the most spectacular cases are in quasars with strong radio jets inclined to the line of sight, they are also almost as common in radio-quiet quasars. Theories of the origin of the peaks are reviewed and it is argued that the displaced peaks are most likely produced by the supermassive binary model. The separations of the peaks in the 3C 390.3-type objects are consistent with orientation-dependent "unified models" of quasar activity. If the supermassive binary model is correct, all members of "the jet set" (astrophysical objects showing jets) could be binaries.Comment: 31 pages, PostScript, missing figure is in ApJ 464, L105 (see http://www.aas.org/ApJ/v464n2/5736/5736.html

Discovery and Characterization of Peptide Inhibitors for Calcium and Integrin Binding Protein 1

Calcium and integrin binding protein 1 (CIB1) is an EF-hand-containing, small intracellular protein that has recently been implicated in cancer cell survival and proliferation. In particular, CIB1 depletion significantly impairs tumor growth in triple-negative breast cancer (TNBC). Thus, CIB1 is a potentially attractive target for cancer chemotherapy that has yet to be validated by a chemical probe. To produce a probe molecule to the CIB1 helix 10 (H10) pocket and demonstrate that it is a viable target for molecular intervention, we employed random peptide phage display to screen and select CIB1-binding peptides. The top peptide sequence selected, UNC10245092, was produced synthetically, and binding to CIB1 was confirmed by isothermal titration calorimetry (ITC) and a time-resolved fluorescence resonance energy transfer (TR-FRET) assay. Both assays showed that the peptide bound to CIB1 with low nanomolar affinity. CIB1 was cocrystallized with UNC10245092, and the 2.1 Å resolution structure revealed that the peptide binds as an α-helix in the H10 pocket, displacing the CIB1 C-terminal H10 helix and causing conformational changes in H7 and H8. UNC10245092 was further derivatized with a C-terminal Tat-derived cell penetrating peptide (CPP) to demonstrate its effects on TNBC cells in culture, which are consistent with results of CIB1 depletion. These studies provide a first-in-class chemical tool for CIB1 inhibition in cell culture and validate the CIB1 H10 pocket for future probe and drug discovery efforts

A review of diagnostic and functional imaging in headache

The neuroimaging of headache patients has revolutionised our understanding of the pathophysiology of primary headaches and provided unique insights into these syndromes. Modern imaging studies point, together with the clinical picture, towards a central triggering cause. The early functional imaging work using positron emission tomography shed light on the genesis of some syndromes, and has recently been refined, implying that the observed activation in migraine (brainstem) and in several trigeminal-autonomic headaches (hypothalamic grey) is involved in the pain process in either a permissive or triggering manner rather than simply as a response to first-division nociception per se. Using the advanced method of voxel-based morphometry, it has been suggested that there is a correlation between the brain area activated specifically in acute cluster headache — the posterior hypothalamic grey matter — and an increase in grey matter in the same region. No structural changes have been found for migraine and medication overuse headache, whereas patients with chronic tension-type headache demonstrated a significant grey matter decrease in regions known to be involved in pain processing. Modern neuroimaging thus clearly suggests that most primary headache syndromes are predominantly driven from the brain, activating the trigeminovascular reflex and needing therapeutics that act on both sides: centrally and peripherally

Linking soil microbial community structure to potential carbon mineralization: A continental scale assessment of reduced tillage

Potential carbon mineralization (Cmin) is a commonly used indicator of soil health, with greater Cmin values interpreted as healthier soil. While Cmin values are typically greater in agricultural soils managed with minimal physical disturbance, the mechanisms driving the increases remain poorly understood. This study assessed bacterial and archaeal community structure and potential microbial drivers of Cmin in soils maintained under various degrees of physical disturbance. Potential carbon mineralization, 16S rRNA sequences, and soil characterization data were collected as part of the North American Project to Evaluate Soil Health Measurements (NAPESHM). Results showed that type of cropping system, intensity of physical disturbance, and soil pH influenced microbial sensitivity to physical disturbance. Furthermore, 28% of amplicon sequence variants (ASVs), which were important in modeling Cmin, were enriched under soils managed with minimal physical disturbance. Sequences identified as enriched under minimal disturbance and important for modeling Cmin, were linked to organisms which could produce extracellular polymeric substances and contained metabolic strategies suited for tolerating environmental stressors. Understanding how physical disturbance shapes microbial communities across climates and inherent soil properties and drives changes in Cmin provides the context necessary to evaluate management impacts on standardized measures of soil microbial activity

Ultra-Fast Flash Observatory: Fast Response Space Missions for Early Time Phase of Gamma Ray Bursts

One of the unexplored domains in the study of gamma-ray bursts (GRBs) is the early time phase of the optical light curve. We have proposed Ultra-Fast Flash Observatory (UFFO) to address this question through extraordinary opportunities presented by a series of small space missions. The UFFO is equipped with a fast-response Slewing Mirror Telescope that uses a rapidly moving mirror or mirror array to redirect the optical beam rather than slewing the entire spacecraft or telescope to aim the optical instrument at the GRB position. The UFFO will probe the early optical rise of GRBs with sub-second response, for the first time, opening a completely new frontier in GRB and transient studies. Its fast response measurements of the optical emission of dozens of GRB each year will provide unique probes of the burst mechanism and test the prospect of GRB as a new standard candle, potentially opening up the z > 10 universe. We describe the current limit in early photon measurements, the aspects of early photon physics, our soon-to-be-launched UFFO-pathfinder mission, and our next planned mission, the UFFO-100

The genera Melanothamnus Bornet & Falkenberg and Vertebrata S.F. Gray constitute well-defined clades of the red algal tribe Polysiphonieae (Rhodomelaceae, Ceramiales).

Polysiphonia is the largest genus of red algae, and several schemes subdividing it into smaller taxa have been proposed since its original description. Most of these proposals were not generally accepted, and currently the tribe Polysiphonieae consists of the large genus Polysiphonia (190 species), the segregate genus Neosiphonia (43 species), and 13 smaller genera (< 10 species each). In this paper, phylogenetic relationships of the tribe Polysiphonieae are analysed, with particular emphasis on the genera Carradoriella, Fernandosiphonia, Melanothamnus, Neosiphonia, Polysiphonia sensu stricto, Streblocladia and Vertebrata. We evaluated the consistency of 14 selected morphological characters in the identified clades. Based on molecular phylogenetic (rbcL and 18S genes) and morphological evidence, two speciose genera are recognized: Vertebrata (including the type species of the genera Ctenosiphonia, Enelittosiphonia, Boergeseniella and Brongniartella) and Melanothamnus (including the type species of the genera Fernandosiphonia and Neosiphonia). Both genera are distinguished from other members of the Polysiphonieae by synapomorphic characters, the emergence of which could have provided evolutionarily selective advantages for these two lineages. In Vertebrata trichoblast cells are multinucleate, possibly associated with the development of extraordinarily long, photoprotective, trichoblasts. Melanothamnus has 3-celled carpogonial branches and plastids lying exclusively on radial walls of the pericentral cells, which similarly may improve resistance to damage caused by excessive light. Other relevant characters that are constant in each genus are also shared with other clades. The evolutionary origin of the genera Melanothamnus and Vertebrata is estimated as 75.7-95.78 and 90.7-138.66 Ma, respectively. Despite arising in the Cretaceous, before the closure of the Tethys Seaway, Melanothamnus is a predominantly Indo-Pacific genus and its near-absence from the northeastern Atlantic is enigmatic. The nomenclatural implications of this work are that 46 species are here transferred to Melanothamnus, six species are transferred to Vertebrata and 13 names are resurrected for Vertebrata

A Historiometric Examination of Machiavellianism and a New Taxonomy of Leadership

Although researchers have extensively examined the relationship between charismatic leadership and Machiavellianism (Deluga, 2001; Gardner & Avolio, 1995; House & Howell, 1992), there has been a lack of investigation of Machiavellianism in relation to alternative forms of outstanding leadership. Thus, the purpose of this investigation was to examine the relationship between Machiavellianism and a new taxonomy of outstanding leadership comprised of charismatic, ideological, and pragmatic leaders. Using an historiometric approach, raters assessed Machiavellianism via the communications of 120 outstanding leaders in organizations across the domains of business, political, military, and religious institutions. Academic biographies were used to assess twelve general performance measures as well as twelve general controls and five communication specific controls. The results indicated that differing levels of Machiavellianism is evidenced across the differing leader types as well as differing leader orientation. Additionally, Machiavellianism appears negatively related to performance, though less so when type and orientation are taken into account.Yeshttps://us.sagepub.com/en-us/nam/manuscript-submission-guideline