218 research outputs found
Transport of Diamines by Enterococcus faecalis Is Mediated by an Agmatine-Putrescine Antiporter
A Simple Method to Check the Reliability of Annual Sunspot Number in the Historical Period 1610-1847
A simple method to detect inconsistencies in low annual sunspot numbers based
on the relationship between these values and the annual number of active days
is described. The analysis allowed for the detection of problems in the annual
sunspot number series clustered in a few specific periods and unambiguous,
namely: i) before Maunder minimum, ii) the year 1652 during the Maunder
minimum, iii) the year 1741 in Solar Cycle -1, and iv) the so-called "lost"
solar cycle in 1790s and subsequent onset of the Dalton Minimum.Comment: 15 pages, 3 figures, to be published in Solar Physic
Using Markov Models and Statistics to Learn, Extract, Fuse, and Detect Patterns in Raw Data
Many systems are partially stochastic in nature. We have derived data driven
approaches for extracting stochastic state machines (Markov models) directly
from observed data. This chapter provides an overview of our approach with
numerous practical applications. We have used this approach for inferring
shipping patterns, exploiting computer system side-channel information, and
detecting botnet activities. For contrast, we include a related data-driven
statistical inferencing approach that detects and localizes radiation sources.Comment: Accepted by 2017 International Symposium on Sensor Networks, Systems
and Securit
BRAF mutation-specific promoter methylation of FOX genes in colorectal cancer
Background: Cancer-specific hypermethylation of (promoter) CpG islands is common during the tumorigenesis of colon cancer. Although associations between certain genetic aberrations, such as BRAF mutation and microsatellite instability, and the CpG island methylator phenotype (CIMP), have been found, the mechanisms by which these associations are established are still unclear. We studied genome-wide DNA methylation differences between
Multi-timescale Solar Cycles and the Possible Implications
Based on analysis of the annual averaged relative sunspot number (ASN) during
1700 -- 2009, 3 kinds of solar cycles are confirmed: the well-known 11-yr cycle
(Schwabe cycle), 103-yr secular cycle (numbered as G1, G2, G3, and G4,
respectively since 1700); and 51.5-yr Cycle. From similarities, an
extrapolation of forthcoming solar cycles is made, and found that the solar
cycle 24 will be a relative long and weak Schwabe cycle, which may reach to its
apex around 2012-2014 in the vale between G3 and G4. Additionally, most Schwabe
cycles are asymmetric with rapidly rising-phases and slowly decay-phases. The
comparisons between ASN and the annual flare numbers with different GOES
classes (C-class, M-class, X-class, and super-flare, here super-flare is
defined as X10.0) and the annal averaged radio flux at frequency of 2.84
GHz indicate that solar flares have a tendency: the more powerful of the flare,
the later it takes place after the onset of the Schwabe cycle, and most
powerful flares take place in the decay phase of Schwabe cycle. Some
discussions on the origin of solar cycles are presented.Comment: 8 pages, 4 figure
A new calibrated sunspot group series since 1749: statistics of active day fractions
Although the sunspot-number series have existed since the mid-19th century, they are still the subject of intense debate, with the largest uncertainty being related to the "calibration" of the visual acuity of individual observers in the past. Daisy-chain regression methods are applied to inter-calibrate the observers which may lead to significant bias and error accumulation. Here we present a novel method to calibrate the visual acuity of the key observers to the reference data set of Royal Greenwich Observatory sunspot groups for the period 1900-1976, using the statistics of the active-day fraction. For each observer we independently evaluate their observational thresholds [S_S] defined such that the observer is assumed to miss all of the groups with an area smaller than S_S and report all the groups larger than S_S. Next, using a Monte-Carlo method we construct, from the reference data set, a correction matrix for each observer. The correction matrices are significantly non-linear and cannot be approximated by a linear regression or proportionality. We emphasize that corrections based on a linear proportionality between annually averaged data lead to serious biases and distortions of the data. The correction matrices are applied to the original sunspot group records for each day, and finally the composite corrected series is produced for the period since 1748. The corrected series displays secular minima around 1800 (Dalton minimum) and 1900 (Gleissberg minimum), as well as the Modern grand maximum of activity in the second half of the 20th century. The uniqueness of the grand maximum is confirmed for the last 250 years. It is shown that the adoption of a linear relationship between the data of Wolf and Wolfer results in grossly inflated group numbers in the 18th and 19th centuries in some reconstructions
The homeobox gene MEIS1 is methylated in BRAFp.V600E mutated colon tumors
Development of colorectal cancer (CRC) can occur both via gene mutations in tumor suppressor genes and oncogenes, as well as via epigenetic changes, including DNA methylation. Site-specific methylation in CRC regulates expression of tumor-associated genes. Right-sided colon tumors more frequently have BRAFp.V600E mutations and have higher methylation grades when compared to left-sided malignancies. The aim of this study was to identify DNA methylation changes associated with BRAFp.V600E mutation status. We performed methylation profiling of colon tumor DNA, isolated from frozen sections enriched for epithelial cells by macro-dissection, and from paired healthy tissue. Single gene analyses comparing BRAFp.V600E with BRAF wild type revealed MEIS1 as the most significant differentially methylated gene (log2 fold change: 0.89, false discovery rate-adjusted P-value 2.8*10-9). This finding was validated by methylation-specific PCR that was concordant with the microarray data. Additionally, validation in an independent cohort (n=228) showed a significant association between BRAF p.V600E and MEIS1 methylation (OR: 13.0, 95% CI: 5.2 - 33.0, P<0.0001). MEIS1 methylation was associated with decreased MEIS1 gene expression in both patient samples and CRC cell lines. The same was true for gene expression of a truncated form of MEIS1, MEIS1D27, which misses exon 8 and has a proposed tumor suppression function. To trace the origin of MEIS1 promoter methylation, 14 colorectal tumors were flow-sorted. Four out of eight BRAFp.V600E tumor epithelial fractions (50%) showed MEIS1 promoter methylation, as well as three out of eight BRAFp.V600E stromal fractions (38%). Only one out of six BRAF wild type showed MEIS1 promoter methylation in both the epithelial tumor and stromal fractions (17%). In conclusion, BRAFp.V600E colon tumors showed significant MEIS1 promoter methylation, which was associated with decreased MEIS1 gene expression. Copyright
Historical Sunspot Observations: A Review
Early observations of sunspot were realised by the naked eye. Possible
utilization of these records for studying the long-term change in the Sun is
discussed here. Other historical sunspot observations with camera obscuras are
also discussed. Moreover, the best record of the behaviour of the Sun exists
for the last four centuries thanks to the observations of sunspots with
telescope. These observations should allow us to know the number, position, and
area of sunspots as well as some relevant episodes (Maunder Minimum, optical
flares, etc.). Rudolf Wolf developed the first reconstruction of solar activity
in the 19th century. The next reconstruction was made by Hoyt and Schatten in
1998 by improving the database and using a new methodological approach. Here
some mistakes, pending tasks and minor improvements are discussed.Comment: 19 pages, 6 figures, to be published in Adv. Space Re
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