High pCO 2 levels affect metabolic rate, but not feeding behavior and fitness, of farmed giant mussel Choromytilus chorus

Indexación: Scopus.Acknowledgements. We thank Luisa Saavedra and Araceli Rodriguez-Romero for their help in the field and during laboratory activities. We also acknowledge Laura Ramajo for help with AT estimations. Emily Giles Neill provided valuable comments that greatly improved the manuscript. Special thanks are due to the reviewers and the editor for very constructive comments on the manuscript. This study was supported by the Millennium Nucleus Center for the Study of Multiple drivers on Marine Socio-Ecological Systems (MUSELS) funded by MINECON NC120086, PIA CONICYT ACT-172037 and FONDECYT grant nos. 1140938 and 1140092 to N.A.L. and M.A.L.Mar Ecol Prog Ser 454: 65−74 Findlay HS, Wood HL, Kendall MA, Spicer JI, Twitchett RJ, Widdicombe S (2009) Calcification, a physiological pro-cess to be considered in the context of the whole organ-ism. 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Reg Environ Change 14(Suppl 1): 19−30 Sabine C, Feely RA, Gruber N, Key RM and others (2004) The oceanic sink of anthropogenic CO2. Science 305: 367–371 SERNAPESCA (Servicio Nacional de Pesca y Acuicultura) (2014) Anuarios estadísticos del Servicio Nacional de Pesca y Acuicultura. www.sernapesca.cl Solórzano L (1969) Determination of ammonia in natural waters by the phenolhypochlorite method. Limnol Oce - anogr 14: 799−801 Thomsen J, Melzner F (2010) Moderate seawater acidifica-tion does not elicit long-term metabolic depression in the blue mussel Mytilus edulis. Mar Biol 157: 2667−2676 Thomsen J, Casties I, Pansch C, Körtzinger A, Melzner F (2013) Food availability outweighs ocean acidification effects in juvenile Mytilus edulis: laboratory and field experiments. Glob Change Biol 19: 1017−1027 Thomsen J, Stapp LS, Haynert K, Schade H, Danelli M, Lannig G, Melzner F (2017) Naturally acidified habitat selects for ocean acidification-tolerant mussels. Sci Adv 3: e1602411 Toro B, Navarro JM, Palma-Fleming H (2003) Relationship between bioenergetics responses and organic pollutants in the giant mussel, Choromytilus chorus (Mollusca: Mytilidae). Aquat Toxicol 63: 257−269 Torres R, Pantoja S, Harada N, González HE, Daneri G, Frangopulos M, Fukasawa M (2011) Air-sea CO2 fluxes along the coast of Chile: from CO2 outgassing in central northern upwelling waters to CO2 uptake in southern Patagonian fjords. J Geophys Res 116: C09006 Torres R, Manriquez PH, Duarte C, Navarro JM, Lagos NA, Vargas CA, Lardies MA (2013) Evaluation of a semi - automatic system for long-term seawater carbonate chemistry manipulation. Rev Chil Hist Nat 86: 443−451 Vargas CA, Aguilera V, Martín V, Manríquez P and others (2015) CO2-driven ocean acidification disrupts the filter feeding behavior in Chilean gastropod and bivalve spe-cies from different geographic localities. Estuaries Coasts 38: 1163−1177 Vargas CA, Lagos NA, Lardies MA, Duarte C and others (2017) Species-specific responses to ocean acidification should account for local adaptation and adaptive plasti-city. Nature Ecol Evol 1: 0084 Vargas CA, Cuevas LA, Silva N, Gonzalez HE, Pol-Holz D, Narvaez DA (2018) Influence of glacier melting and river discharges on the nutrient distribution and DIC recycling in the southern Chilean Patagonia. J Geophys Res Bio-geosci 123: 256−270 Velasco LA, Navarro JM (2003) Energetic balance of infau-nal (Mulinia edulis King, 1831) and epifaunal (Mytilus chilensis Hupé, 1854) bivalves in response to wide varia-tions in concentration and quality of seston. J Exp Mar Biol Ecol 296: 79−92 Vihtakari M, Hendriks IE, Holding J, Renaud PE, Duarte CM, Havenhand JN (2013) Effects of ocean acidification and warming on sperm activity and early life stages of the Mediterranean mussel (Mytilus galloprovincialis). Water 5: 1890−1915 Wang Y, Li L, Hu M, Lu W (2015) Physiological energetic of the thick shell mussel Mytilus coruscus exposed to sea-water acidification and thermal stress. Sci Total Environ 514: 261−272 White MM, McCorkle DC, Mullineaux LS, Cohen AL (2013)Benthic habitats such as intertidal areas, sandy or rocky shores, upwelling zones, and estuaries are characterized by variable environmental conditions. This high variability of environmental stressors such as temperature, salinity, and pH/pCO 2 levels have been shown to impose restrictions on organismal performance. The giant mussel Choromytilus chorus forms intertidal and subtidal mussel beds in estuarine zones associated with fjords occurring in southern Chile and is an important aquacultural resource in Patagonia. In this study, we estimated the sensitivity of physiological traits and energy balance of C. chorus juveniles exposed to 3 pCO 2 treatments (500, 750, and 1200 μatm) for 30 d. Results showed that in acidified, high pCO 2 conditions, C. chorus juveniles had increased metabolic rates; however, other physiological traits (clearance and ingestion rates, ammonia excretion, absorption efficiency, growth rate, biomass production, net calcification, and dissolution rates) were not affected. These results suggest that when subjected to acidification, the adaptive response of C. chorus triggers tradeoffs among physiological traits that favor sustained feeding and growth in order to combat increased metabolic stress. As has been reported for other marine organisms, chronic exposure to variable pH/pCO 2 in their native habitats, such as estuarine zones, could explain the differential acclimatization capacity of giant mussels to cope with the increase in pCO 2 . Additionally, the fact that the mussels did not suffer from mortality indicates that increased pCO 2 levels may have chronic, but not lethal, effects on this species under these experimental conditions. © The authors 2017.https://www.int-res.com/abstracts/aei/v10/p267-278

Ant colony optimized planning for unmanned surface marine vehicles

This paper presents some results achieved from a preliminary study on the use of the Ant Colony Algorithm to plan feasible optimal or suboptimal trajectories for an autonomous ship manoeuvring. The scenario, for this preliminary work, comprises only open sea manoeuvres. The goal involves obtaining the least time consuming ship trajectory between to points, departing from the start point with arbitrary initial speed and attitude values and arriving to the end point with predefined speed and attitude values. The specific dynamic of the ship imposes typical restrictions to its manoeuvrability. In the present case, the non-holonomicity, the rate speed/turn radius, and the imposed forward-only propulsion of the ship make up the main restrictions to the ship movement. For long distances, the problem could be tackled as a classical navigation problem, in which, for the most part of the ship trajectory, techniques such inertial navigation should be enough. The problem arises at short distances when it becomes a manoeuvring problem. In this case to obtain a optimal, --in some cases just a feasible--, trajectory could be a difficult problem.Peer Reviewe

Comparative RNAseq analysis of backfat tissue from local pig breeds.

Alentejano (AL) and Bísaro (BI) are the main local pig breeds in Portugal, but have no information comparing their transcriptomic activity. AL belongs to the Iberian branch, presenting lower growth rates, precociously high adipogenic activity and higher levels of unsaturated fatty acids (FAs) while BI pig is from the Celtic group, sharing ancestors with higher growth rate and leaner commercial breeds. This work intended to explore the genome function of AL and BI to better understand the underlying physiological mechanisms associated with body fat accretion, lipid composition and meat quality. Dorsal subcutaneous fat (DSF) samples were collected from AL and BI fattening pigs, with ~150kg BW at slaughter. Total RNA was obtained and sequenced for transcriptome analysis. Bioinformatic analyses using three different tools (Cufflinks, EdgeR and DESeq2) were performed. A total of 367, 137 and 155 differentially expressed genes (DEGs) (q-value0.8) were found using the Cufflinks, EdgeR and DESeq2 pipelines, respectively, between AL and BI DSF samples. EdgeR and DESeq2 shared a total 121 DEGs (~71% overlap) while Cufflinks showed divergent results (2.7% overlap with EdgeR and 5.5% with DESeq2). A functional enrichment analysis of the candidate DEGs was performed using Ingenuity Pathway Analysis. Synthesis of lipid, depletion of glycogen, mass of organism and accumulation of oleic acid were revealed as main involved functions (p-value<0.05) though no directional activation state was observed (-2<Zscore<2). Potential upstream regulators that explain the obtained results such as TCF7L2 and RIPK2 were predicted to be activated and inhibited in AL, respectively. Moreover, 4 causal networks with RIT2, KL, FLCN and RIPK2 as master regulators were inhibited in AL while another with PPARGC1B was activated. These results present the first high-throughput transcriptomic data involving these local breeds and can help explain the metabolic differences that occur in the adipose tissue and shed light into specific meat quality traits

Transcriptomic profiling of skeletal muscle reveals candidate genes influencing muscle growth and associated lipid composition in Portuguese local pig breeds

Gene expression is one of the main factors to influence meat quality by modulating fatty acid metabolism, composition, and deposition rates in muscle tissue. This study aimed to explore the transcriptomics of the Longissimus lumborum muscle in two local pig breeds with distinct genetic background using next-generation sequencing technology and Real-Time qPCR. RNA-seq yielded 49 differentially expressed genes between breeds, 34 overexpressed in the Alentejano (AL) and 15 in the Bísaro (BI) breed. Specific slow type myosin heavy chain components were associated with AL (MYH7) and BI (MYH3) pigs, while an overexpression of MAP3K14 in AL may be associated with their lower loin proportion, induced insulin resistance, and increased inflammatory response via NFkB activation. Overexpression of RUFY1 in AL pigs may explain the higher intramuscular (IMF) content via higher GLUT4 recruitment and consequently higher glucose uptake that can be stored as fat. Several candidate genes for lipid metabolism, excluded in the RNA-seq analysis due to low counts, such as ACLY, ADIPOQ, ELOVL6, LEP and ME1 were identified by qPCR as main gene factors defining the processes that influence meat composition and quality. These results agree with the fatter profile of the AL pig breed and adiponectin resistance can be postulated as responsible for the overexpression of MAP3K140s coding product NIK, failing to restore insulin sensitivity

Identification of differentially expressed key genes of Longissimus lumborum samples from Portuguese Alentejano and Bísaro local pig breeds

Most of the swine industry nowadays is about productive and profitable fast-growing lean breeds raised under intensive conditions leading to meat and fat rich in saturated fatty acids. The Portuguese local Alentejano (AL) and Bísaro (BI) pig breeds present high intramuscular fat (IMF) content which contributes to highly appreciated pork products. These breeds have different ancestors: AL belongs to the Iberian group, presenting lower growth rates and higher lipid accretion and unsaturated fatty acids level when compared to BI, which belongs to the Celtic group, sharing ancestors with leaner breeds such as Large White and Landrace. The goal of this work was to compare the muscle gene expression profiles of AL and BI pig breeds to better understand the influence of the genetic background in the main metabolic processes occurring in the Longissimus lumborum (LL) muscle, namely in terms of lipid synthesis, muscle tissue formation, protein synthesis and cell proliferation. LL samples were obtained at slaughter, from adult AL and BI pigs with ~150kg body weight. Total RNA was extracted and sequenced for a transcriptome comparison analysis. A total of 250 genes were found to be differentially expressed (DE) in LL samples (q<0.05) conditional on breed, with 174 DE genes up-regulated in AL (log2(fold_change) = 0.65 to 7.03) and 76 in BI (log2(fold_change) = 0.63 to 4.53). Genes related to skeletal muscle development and function, such as MYH3, MYH13 or ACTN4, were significantly up-regulated in BI when compared to AL, which is in agreement with the higher muscle mass of the former breed. Genes involved in lipid metabolism were up-regulated in AL, including SCD (q=0.05), responsible for catalysing the reaction that introduces the first double bond into saturated fatty acyl-CoA substrates, which agrees with the higher unsaturation of fat tissues generally associated with the former breed. A functional enrichment analysis (metabolic pathways and GO enrichment) was performed for the DE genes and the identified functions included tissue development, cellular growth and proliferation, quantity of connective tissue and lipid metabolism. Potential regulators found that explain the observed gene expression changes in the dataset included molecules such as: ADORA2A, CEBPA, SMAD3 and PPARG (predicted to be activated in AL); HDAC and ASXL1 (predicted to be inhibited in AL)

Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds

When compared to modern lean-type breeds, Portuguese local Alentejano (AL) and Bísaro (BI) pig breeds present a high potential for subcutaneous and intramuscular fat (IMF) deposition which contributes for better meat quality. The aim of this work was to explore the genome function to better understand the underlying physiological mechanisms associated with body fat accretion. Dorsal subcutaneous fat samples were collected at slaughter from adult animals (n = 4 for each breed) with ~150 kg body weight. Total RNA was obtained and sequenced for transcriptome analysis using DESeq2. A total of 458 di erentially expressed (DE) genes (q-value < 0.05) were identified, with 263 overexpressed in AL and 195 in BI. Key genes involved in de novo fatty acid biosynthesis, elongation and desaturation were upregulated in AL such as ACLY, FASN, ME1, ELOVL6 and SCD. A functional enrichment analysis of the DE genes was performed using Ingenuity Pathway Analysis. Cholesterol synthesis is suggested to be higher in AL via SREBF2, SCAP and PPARG, while lipolytic activity may be more active in BI through GH and AMPK signalling. Increased signalling of CD40 together with the predicted activation of INSIG1 and INSIG2 in BI suggests that this breed is more sensitive to insulin whereas the AL is less sensitive like the Iberian breed

Using the FVB strain of mice for the evaluation of clinical and experimental ketamine (IP) associated with phenothiazines, benzodiazepines and α2-agonists

El objetivo de este trabajo ha consistido en evaluar el estado fisiológico de los animales mediante el control de las frecuencias respiratoria y cardiaca así como la tasa de saturación de oxígeno durante la anestesia con ketamina asociada a otros fármacos. Para ello hemos utilizado 40 ratones FVB consanguíneos (20 machos y 20 hembras) de 11 semanas de edad, a los que se les administró por vía intraperitoneal ketamina asociada a un depresor del sistema nervioso central: acepromazina, diazepam, medetomidina, midazolam o xilazina. Obtuvimos resultados que difirieron mucho entre sexos, concluyendo que en machos los mejores resultados obtenidos fueron con la asociación a los α2-agonistas, mientras que en las hembras, al menos a las dosis empleadas, no pudimos afirmar que ninguna de las asociaciones fuese óptima.The aim of this work has been to evaluate the physiological status of animals by controlling the respiratory rate and heart rate and oxygen saturation during anesthesia with ketamine in combination with other drugs. We have used 40 consanguine FVB mice (20 males and 20 females) from 11 weeks of age, who were administered intraperitoneally with a ketamine-associated central nervous system depressant:acepromazina, diazepam, medetomidine, midazolam or xylazine. We obtained results that differed greatly between the sexes, in males, concluding that the best results were obtained with the association of α2- agonists, while in females, at least at the doses employed, did not say that none of the associations were optima

alpha-particle production in the scattering of 6He by 208Pb at energies around the Coulomb barrier

New experimental data from the scattering of 6He+208Pb at energies around and below the Coulomb barrier are presented. The yield of breakup products coming from projectile fragmentation is dominated by a strong group of $\alpha$ particles. The energy and angular distributions of this group have been analyzed and compared with theoretical calculations. This analysis indicates that the $\alpha$ particles emitted at backward angles in this reaction are mainly due to two-neutron transfer to weakly bound states of the final nucleus.Comment: 20 pages, 5 figures. Nuclear Physics A792 (2007) 2-1

Global diversity and biogeography of deep-sea pelagic prokaryotes

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