Reversible protein precipitation to ensure stability during encapsulation within PLGA microspheres

Proteins were precipitated to ensure their stability upon subsequent encapsulation within PLGA microspheres. Spherical, nanosized protein particles were formed by the addition of a salt (sodium chloride) and a water-miscible organic solvent (glycofurol) to protein solutions. Various process parameters were modified to optimize the precipitation efficiency of four model proteins: lysozyme, alpha-chymotrypsin, peroxidase and beta-galactosidase. As monitored by enzymatic activity measurement of the rehydrated particles, conditions to obtain more than 95% of reversible precipitates were defined for each protein. The study of the structure of the rehydrated particles by absorbance spectroscopy, fluorescence spectroscopy and circular dichroism showed an absence of structural-perturbation after precipitation. Protein particles were then microencapsulated within PLGA microspheres using s/o/w technique. The average encapsulation yield was around 80% and no loss of protein activity occurred after the encapsulation step. Additionally, a lysozyme in vitro release study showed that all of the released lysozyme was biologically active. This method of protein precipitation is appropriate for the encapsulation in PLGA microspheres of various proteins without inactivation

Tame Functions with strongly isolated singularities at infinity: a tame version of a Parusinski's Theorem

Let f be a definable function, enough differentiable. Under the condition of having strongly isolated singularities at infinity at a regular value c we give a sufficient condition expressed in terms of the total absolute curvature function to ensure the local triviality of the function f over a neighbourhood of c and doing so providing the tame version of Parusinski's Theorem on complex polynomials with isolated singularities at infinity.Comment: 20 page

Evidence of Final-State Suppression of High-p_T Hadrons in Au + Au Collisions Using d + Au Measurements at RHIC

Transverse momentum spectra of charged hadrons with ${p_{T} <}$ 6 GeV/c have been measured near mid-rapidity (0.2 $< \eta <$ 1.4) by the PHOBOS experiment at RHIC in Au + Au and d + Au collisions at ${\sqrt{s_{_{NN}}} = \rm {200 GeV}}$. The spectra for different collision centralities are compared to ${p + \bar{p}}$ collisions at the same energy. The resulting nuclear modification factor for central Au + Au collisions shows evidence of strong suppression of charged hadrons in the high-$p_{T}$ region (${>2}$ GeV/c). In contrast, the d + Au nuclear modification factor exhibits no suppression of the high-$p_{T}$ yields. These measurements suggest a large energy loss of the high-$p_{T}$ particles in the highly interacting medium created in the central Au + Au collisions. The lack of suppression in d + Au collisions suggests that it is unlikely that initial state effects can explain the suppression in the central Au + Au collisions.Comment: 3 pages, 4 figures, International Europhysics Conference on High Energy Physics EPS (July 17th-23rd 2003) in Aachen, German

Identified particles in Au+Au collisions at sqrt{s_NN} = 200 GeV

The yields of identified particles have been measured at RHIC for Au+Au collisions at sqrt{s_NN} = 200 GeV using the PHOBOS spectrometer. The ratios of antiparticle to particle yields near mid-rapidity are presented. The first measurements of the invariant yields of charged pions, kaons and protons at very low transverse momenta are also shown.Comment: 4 pages, 4 figures, Contribution to Quark Matter 2002, Nantes, France, July 200

Universal Behavior of Charged Particle Production in Heavy Ion Collisions

The PHOBOS experiment at RHIC has measured the multiplicity of primary charged particles as a function of centrality and pseudorapidity in Au+Au collisions at sqrt(s_NN) = 19.6, 130 and 200 GeV. Two kinds of universal behavior are observed in charged particle production in heavy ion collisions. The first is that forward particle production, over a range of energies, follows a universal limiting curve with a non-trivial centrality dependence. The second arises from comparisons with pp/pbar-p and e+e- data. N_tot/(N_part/2) in nuclear collisions at high energy scales with sqrt(s) in a similar way as N_tot in e+e- collisions and has a very weak centrality dependence. This feature may be related to a reduction in the leading particle effect due to the multiple collisions suffered per participant in heavy ion collisions.Comment: 4 Pages, 5 Figures, contributed to the Proceedings of Quark Matter 2002, Nantes, France, 18-24 July 200

Recent Results from PHOBOS at RHIC

The PHOBOS experiment at RHIC has recorded measurements for Au-Au collisions spanning nucleon-nucleon center-of-mass energies from 19.6 GeV to 200 GeV. Global observables such as elliptic flow and charged particle multiplicity provide important constraints on model predictions that characterize the state of matter produced in these collisions. The nearly 4 pi acceptance of the PHOBOS experiment provides excellent coverage for complete flow and multiplicity measurements. Results including beam energy and centrality dependencies are presented and compared to elementary systems.Comment: 4 pages, 4 figures, proceedings from PANIC02 in Osaka, Japa

Global Observations from PHOBOS

Particle production in Au+Au collisions has been measured in the PHOBOS experiment at RHIC for a range of collision energies. Three empirical observations have emerged from this dataset which require theoretical examination. First, there is clear evidence of limiting fragmentation. Namely, particle production in central Au+Au collisions, when expressed as $dN/d\eta'$ ($\eta' \equiv \eta-y_{beam}$), becomes energy independent at high energy for a broad region of $\eta'$ around $\eta'=0$. This energy-independent region grows with energy, allowing only a limited region (if any) of longitudinal boost-invariance. Second, there is a striking similarity between particle production in e+e- and Au+Au collisions (scaled by the number of participating nucleon pairs). Both the total number of produced particles and the longitudinal distribution of produced particles are approximately the same in e+e- and in scaled Au+Au. This observation was not predicted and has not been explained. Finally, particle production has been found to scale approximately with the number of participating nucleon pairs for $N_{part}>65$. This scaling occurs both for the total multiplicity and for high \pT particles (3 <\pT< 4.5 GeV/c).Comment: QM2002 plenary talk, 10 pages, 11 figure

High prevalence of PRPH2 in autosomal dominant retinitis pigmentosa in france and characterization of biochemical and clinical features.

PURPOSE: To assess the prevalence of PRPH2 in autosomal dominant retinitis pigmentosa (adRP), to report 6 novel mutations, to characterize the biochemical features of a recurrent novel mutation, and to study the clinical features of adRP patients. DESIGN: Retrospective clinical and molecular genetic study. METHODS: Clinical investigations included visual field testing, fundus examination, high-resolution spectral-domain optical coherence tomography (OCT), fundus autofluorescence imaging, and electroretinogram (ERG) recording. PRPH2 was screened by Sanger sequencing in a cohort of 310 French families with adRP. Peripherin-2 protein was produced in yeast and analyzed by Western blot. RESULTS: We identified 15 mutations, including 6 novel and 9 previously reported changes in 32 families, accounting for a prevalence of 10.3% in this adRP population. We showed that a new recurrent p.Leu254Gln mutation leads to protein aggregation, suggesting abnormal folding. The clinical severity of the disease in examined patients was moderate with 78% of the eyes having 1-0.5 of visual acuity and 52% of the eyes retaining more than 50% of the visual field. Some patients characteristically showed vitelliform deposits or macular involvement. In some families, pericentral RP or macular dystrophy were found in family members while widespread RP was present in other members of the same families. CONCLUSIONS: The mutations in PRPH2 account for 10.3% of adRP in the French population, which is higher than previously reported (0%-8%) This makes PRPH2 the second most frequent adRP gene after RHO in our series. PRPH2 mutations cause highly variable phenotypes and moderate forms of adRP, including mild cases, which could be underdiagnosed