Leaf morphology in cowpea [Vigna unguiculata (L.) Walp]: QTL analysis, physical mapping and identifying a candidate gene using synteny with model legume species

Cowpea [Vigna unguiculata (L.) Walp] exhibits a considerable variation in leaf shape. Although cowpea is mostly utilized as a dry grain and animal fodder crop, cowpea leaves are also used as a high-protein pot herb in many countries of Africa

Asymptotic Freedom for Non-Relativistic Confinement

Some aspects of asymptotic freedom are discussed in the context of a simple two-particle non-relativisitic confining potential model. In this model asymptotic freedom follows from the similarity of the free-particle and bound state radial wave functions at small distances and for the same angular momentum and the same large energy. This similarity, which can be understood using simple quantum mechanical arguments, can be used to show that the exact response function approaches that obtained when final state interactions are ignored. A method of calculating corrections to this limit is given and explicit examples are given for the case of the harmonic oscillator.Comment: 16 pages, 5 figures, RevTex

Identification of markers associated with bacterial blight resistance loci in cowpea (Vigna unguiculata (L.) Walp.)

Cowpea bacterial blight (CoBB), caused by Xanthomonas axonopodis pv. vignicola (Xav), is a worldwide major disease of cowpea [Vigna unguiculata (L.) Walp.]. Among different strategies to control the disease including cultural practices, intercropping, application of chemicals, and sowing pathogen-free seeds, planting of cowpea genotypes with resistance to the pathogen would be the most attractive option to the resource poor cowpea farmers in sub-Saharan Africa. Breeding resistance cultivars would be facilitated by marker-assisted selection (MAS). In order to identify loci with effects on resistance to this pathogen and map QTLs controlling resistance to CoBB, eleven cowpea genotypes were screened for resistance to bacterial blight using 2 virulent Xav18 and Xav19 strains isolated from Kano (Nigeria). Two cowpea genotypes Danila and Tvu7778 were identified to contrast in their responses to foliar disease expression following leaf infection with pathogen. A set of recombinant inbred lines (RILs) comprising 113 individuals derived from Danila (resistant parent) and Tvu7778 (susceptible parent) were infected with CoBB using leaf inoculation method. The experiments were conducted under greenhouse conditions (2007 and 2008) and disease severity was visually assessed using a scale where 0 = no disease and 4 = maximum susceptibility with leaf drop. A single nucleotide polymorphism (SNP) genetic map with 282 SNP markers constructed from the same RIL population was used to perform QTL analysis. Using Kruskall-Wallis and Multiple-QTL model of MapQTL 5, three QTLs, CoBB-1, CoBB-2 and CoBB-3 were identified on linkage group LG3, LG5 and LG9 respectively showing that potential resistance candidate genes cosegregated with CoBB resistance phenotypes. Two of the QTLs CoBB-1, CoBB-2 were consistently confirmed in the two experiments accounting for up to 22.1 and to 17.4% respectively for the first and second experiments. Whereas CoBB-3 was only discovered for the first experiment (2007) with less phenotypic variation explained of about 10%. Our results represent a resource for molecular marker development that can be used for marker assisted selection of bacterial blight resistance in cowpe

Spin-dependent Parton Distributions from Polarized Structure Function Data

In the past year, polarized deep inelastic scattering experiments at CERN and SLAC have obtained structure function measurements off proton, neutron and deuteron targets at a level of precision never before achieved. The measurements can be used to test the Bjorken and Ellis-Jaffe sum rules, and also to obtain information on the parton distributions in polarized nucleons. We perform a global leading-order QCD fit to the proton deep inelastic data in order to extract the spin-dependent parton distributions. By using parametric forms which are consistent with theoretical expectations at large and small $x$, we find that the quark distributions are now rather well constrained. We assume that there is no significant intrinsic polarization of the strange quark sea. The data are then consistent with a modest amount of the proton's spin carried by the gluon, although the shape of the gluon distribution is not well constrained, and several qualitatively different shapes are suggested. The spin-dependent distributions we obtain can be used as input to phenomenological studies for future polarized hadron-hadron and lepton-hadron colliders.Comment: 23 pages, DTP/94/3

Genetic architecture of delayed senescence, biomass, and grain yield under drought stress in cowpea

CowpeaThe stay-green phenomenon is a key plant trait with wide usage in managing crop production under limited water conditions. This trait enhances delayed senescence, biomass, and grain yield under drought stress. In this study we sought to identify QTLs in cowpea (Vigna unguiculata) consistent across experiments conducted in Burkina Faso, Nigeria, Senegal, and the United States of America under limited water conditions. A panel of 383 diverse cowpea accessions and a recombinant inbred line population (RIL) were SNP genotyped using an Illumina 1536 GoldenGate assay. Phenotypic data from thirteen experiments conducted across the four countries were used to identify SNP-trait associations based on linkage disequilibrium association mapping, with bi-parental QTL mapping as a complementary strategy. We identified seven loci, five of which exhibited evidence suggesting pleiotropic effects (stay-green) between delayed senescence, biomass, and grain yield. Further, we provide evidence suggesting the existence of positive pleiotropy in cowpea based on positively correlated mean phenotypic values (0.34, r ,0.87) and allele effects (0.07, r ,0.86) for delayed senescence and grain yield across three African environments. Three of the five putative stay-green QTLs, Dro-1, 3, and 7 were identified in both RILs and diverse germplasm with resolutions of 3.2 cM or less for each of the three loci, suggesting that these may be valuable targets for marker-assisted breeding in cowpea. Also, the co-location of early vegetative delayed senescence with biomass and grain yield QTLs suggests the possibility of using delayed senescence at the seedling stage as a rapid screening tool for post-flowering drought tolerance in cowpea breeding. BLAST analysis using EST sequences harboring SNPs with the highest associations provided a genomic context for loci identified in this study in closely related common bean (Phaseolus vulgaris) and soybean (Glycine max) reference genomes

Axial vector form factor of nucleons in a light-cone diquark model

The nucleon axial vector form factor is investigated in a light-cone quark spectator diquark model, in which Melosh rotations are applied to both the quark and vector diquark. It is found that this model gives a very good description of available experimental data and the results have very little dependence on the parameters of the model. The relation between the nucleon axial constant and the anomalous magnetic moment of nucleons is also discussed.Comment: 8 pages, Revtex4, 1 figure, version to be published in Phys. Rev.

Two-photon final states in peripheral heavy ion collisions

We discuss processes leading to two photon final states in peripheral heavy ion collisions at RHIC. Due to the large photon luminosity we show that the continuum subprocess $\gamma \gamma \to \gamma \gamma$ can be observed with a large number of events. We study this reaction when it is intermediated by a resonance made of quarks or gluons and discuss its interplay with the continuum process, verifying that in several cases the resonant process ovewhelms the continuum one. It is also investigated the possibility of observing a scalar resonance (the $\sigma$ meson) in this process. Assuming for the $\sigma$ the mass and total decay width values recently reported by the E791 Collaboration we show that RHIC may detect this particle in its two photon decay mode if its partial photonic decay width is of the order of the ones discussed in the literature.Comment: 10 pages, 8 figure

Non-equilibrium initial conditions from pQCD for RHIC and LHC

We calculate the initial non-equilibrium conditions from perturbative QCD (pQCD) within Glauber multiple scattering theory for $\sqrt s =200$ AGeV and $\sqrt s =5.5$ ATeV. At the soon available collider energies one will particularly test the small $x$ region of the parton distributions entering the cross sections. Therefore shadowing effects, previously more or less unimportant, will lead to new effects on variables such as particle multiplicities $dN/dy$, transverse energy production $d\bar{E}_T/dy$, and the initial temperature $T_i$. In this paper we will have a closer look on the effects of shadowing by employing different parametrizations for the shadowing effect for valence quarks, sea quarks and gluons. Since the cross sections at midrapidity are dominated by processes involving gluons the amount of their depletion is particularly important. We will therefore have a closer look on the results for $dN/dy$, $d\bar{E}_T/dy$, and $T_i$ by using two different gluon shadowing ratios, differing strongly in size. As a matter of fact, the calculated quantities differ significantly.Comment: typo in ref's removed, ack's added, no change in result

Gene pools and the genetic architecture of domesticated cowpea

Open Access JournalCowpea [Vigna unguiculata (L.) Walp.] is a major tropical legume crop grown in warm to hot areas throughout the world and especially important to the people of sub-Saharan Africa where the crop was domesticated. To date, relatively little is understood about its domestication origins and patterns of genetic variation. In this study, a worldwide collection of cowpea landraces and African ancestral wild cowpea was genotyped with more than 1200 single nucleotide polymorphism markers. Bayesian inference revealed the presence of two major gene pools in cultivated cowpea in Africa. Landraces from gene pool 1 are mostly distributed in western Africa while the majority of gene pool 2 are located in eastern Africa. Each gene pool is most closely related to wild cowpea in the same geographic region, indicating divergent domestication processes leading to the formation of two gene pools. The total genetic variation within landraces from countries outside Africa was slightly greater than within African landraces. Accessions from Asia and Europe were more related to those from western Africa while accessions from the Americas appeared more closely related to those from eastern Africa. This delineation of cowpea germplasm into groups of genetic relatedness will be valuable for guiding introgression efforts in breeding programs and for improving the efficiency of germplasm managemen

Masses of light tetraquarks and scalar mesons in the relativistic quark model

Masses of the ground state light tetraquarks are dynamically calculated in the framework of the relativistic diquark-antidiquark picture. The internal structure of the diquark is taken into account by calculating the form factor of the diquark-gluon interaction in terms of the overlap integral of the diquark wave functions. It is found that scalar mesons with masses below 1 GeV: f_0(600) (\sigma), K^*_0(800) (\kappa), f_0(980) and a_0(980) agree well with the light tetraquark interpretation.Comment: 9 pages, Report-no adde