Nitrogen source on performance of feedlot young bulls fed hydrolyzed sugarcane bagasse diets

Oitenta e um machos não castrados das raças Nelore (27), Canchim (27) e Holandesa (27), com peso médio inicial de 360kg e idade média de 18 meses, foram utilizados para avaliar os efeitos da substituição de fonte de proteína verdadeira (farelo de soja), por nitrogênio não protéico (NNP), uréia ou amiréia (fonte de nitrogênio não protéico de suposta liberação gradativa) sobre o desempenho de bovinos confinados. O delineamento experimental foi o de blocos inteiramente ao acaso com três animais por baia e nove baias por tratamento. Os blocos foram delineados de acordo com o peso inicial e na raça. As rações experimentais continham BTPV (45% MS da dieta) e BIN (5% MS da dieta) como fontes de volumosos e 50% de concentrado. Os tratamentos consistiam de: 1) concentrado contendo farelo de soja (FS); 2) concentrado contendo uréia e 3) concentrado contendo amiréia (A-150S). O consumo de matéria seca (CMS) foi de 8,99; 7,43 e 7,69kg/dia, o ganho de peso diário (GPD) foi de 0,983; 0,368 e 0,404kg/dia e a conversão alimentar (CA) 9,56; 20,14 e 19,54kg MS/kg de ganho para os tratamentos FS, uréia e A-150S, respectivamente. As rações com FS apresentaram proporcionalmente maior (P<0,01) CMS e maior GPD e melhor CA em relação aos tratamentos uréia e A-150S. As rações com uréia e A-150S não diferiram (P>0,05) entre si. A substituição do FS por fontes de NNP reduziu o desempenho de bovino de corte em terminação.Eighty-one young bulls (27 Nellore, 27 Canchim, and 27 Holstein), averaging 18-month-old and weighting 360kg of initial body weight (BW), were used to evaluate the effects of nitrogen sources on feedlot performance. Treatments were assigned in a completely randomized block design using three steers per stall and nine per treatment. Blocks were defined by initial BW and breed. Experimental treatments were: 1) soybean meal, 2) urea, and 3) starea. Diets were isonitrogenous and isoenergetic composed by 50% concentrate and 50% forage (45% hydrolyzed sugarcane bagasse + 5% in natura sugarcane bagasse). Dry matter intakes (DMI) were 8.99, 7.43, and 7.69kg/day, average daily gains (ADG) were 0.983, 0.368, and 0.404kg/day and feed efficiencies were 9.56, 20.14, and 19.54kg DM/kg of gain for soybean meal, urea and starea treatments, respectively for steers fed. Diets with soybean meal showed proportionally higher (P<0.01) DMI, higher average daily gain and better feed efficiency compared to urea and starea diets. No differences (P>0.05) between urea and starea treatments were observed. Replacement of soybean meal by nonprotein nitrogen sources decreased the finishing beef steers performance

Genetic Distance Based On Ssr And Grain Yield Of Inter And Intrapopulational Maize Single Cross Hybrids

The objective of this work was to correlate the genetic distances between the progenitors obtained by microsatellite markers with the grain yield of inter and intrapopulational maize single cross hybrids. Three S 0 populations derived from commercial single cross hybrids were used to obtain 163 hybrids (110 interpopulational and 53 intrapopulational). The two best hybrids and two worst hybrids of each the inter- and intrapopulational crosses were selected and their progenitors maintained through self-pollination of the second ear of each S 0 plant, genotyped with 47 SSRs. The Modified Roger's Distance (MRD) between each pair of S 1 inbred lines, the number of alleles and the polymorphic information content (PIC) of each primer were estimated. The genetic distances between progenitors were correlated with the grain yield of the inter- and intrapopulational hybrids. The number of obtained alleles was 186, with a mean of 3.96 alleles. The PIC varied from 0.49 to 0.80, with a mean of 0.65. The mean genetic distance between all S 1 inbred lines was 0.75, varying from 0.40 to 0.89, indicating the existence of variability between the S 1 inbred lines. The correlation between MRD and grain yield was high and significant for the interpopulational crosses (r = 0.84, P ≤ 0.01) and low and not significant (r = 0.18, P ≥ 0.05) for intrapopulational crosses.5103/04/15507513Ajmone Marsan, P., Castiglioni, P., Fu Sari, F., Kuiper, M., Motto, M., Genetic diversity and its relationship to hybrid performance in maize as revealed by RFLP and AFLP markers (1998) Theor. Appl. Genet., 96, pp. 219-227Árcade, A., Faivre-Rampant, P., Le Guerroué, B., Paques, L.E., Prat, D., Heterozigosity and hybrid performance in larch (1996) Theor. Appl. Genet., 93, pp. 1274-1281Barbosa, A.M.M., Geraldi, I.O., Benchimol, L.L., Garcia, A.A.F., Souza Jr., C.L., Souza, A.P., Relationship of intra and interpopulation tropical maize single cross hybrid performance and genetic distances computed from AFLP and SSR markers (2003) Euphytica, 87, pp. 87-99Benchimol, L.L., Souza Jr., C.L., Garcia, A.A.F., Kono, P.M.S., Mangolin, C.A., Barbosa, A.M.M., Coelho, A.S.G., Souza, A.P., Genetic diversity in tropical maize inbred lines: Heterotic group assignment and hybrid performance determined by RFLP markers (2000) Plant Breed., 119, pp. 491-496Boppenmaier, J., Melchinger, A.E., Brunklaus-Junt, E., Geiger, H.H., Genetic diversity for RFLP in European maize inbreds: Relation to performance of flint x dent crosses for forage traits (1992) Crop Sci., 32, pp. 895-902Butstein, D., White, M., Sholnick, M., David, R.W., Construction of a genetic linkage map in man using restriction fragment length polymorphisms (1980) Am. J. Hum. Genet., 32, pp. 314-331Cheres, M.T., Miller, J.F., Crane, J.M., Knapp, S.J., Genetic distance as a predictor of heterosis and hybrid performance within and between heterotic groups in sunflower (2000) Theor. Appl. Genet., 100, pp. 889-894Cruz, C.D., Shuster, I., (2004) GQMOL: Aplicativo Computacional para Análise de Dados Moleculares e de Suas Associações Com Caracteres Quantitativos. Versão 2004.2.1, , Viçosa. MGDhillon, B.S., Boppenmaier, J., Pollmer, R.G., Hermann, R.G., Melchinger, A.E., Relationship of restriction length polymorphisms among european maize inbreds with ear dry matter yield of their hybrids (1993) Maydica, 38, pp. 245-248Drinic, S.M., Trifunovic, S., Drinic, G., Konstantinov, K., Genetic divergence and its correlation to heterosis in maize as revealed by SSR base markers (2002) Maydica, 47, pp. 1-8Goodman, M.M., Stuber, C.W., Races of maize: VI. Isozyme variation among races of maize in Bolivia (1983) Maydica, 28, pp. 169-187Hallauer, A.R., Miranda Filho, J.B., (1988) Quantitative Genetics in Maize Breeding, 468p. , Iowa State University Press, AmesKruskal, J.B., Multidimensional scaling by optimizing goodness of fit to a no metric hypothesis (1964) Psychometrika, 29, pp. 1-27Lanza, L.L.B., Souza Jr., C.L., Ottoboni, I.M.M., Vieira, M.L.C., Souza, A.P., Genetic distance of inbred lines and prediction of maize single-cross performance using RAPD markers (1997) Theor. Appl. Genet., 94, pp. 1023-1030Le Clerc, V., Bazante, F., Baril, C., Guiard, J., Zhang, D., Assessing temporal changes in genetic diversity of maize varieties using microsatellite markers (2005) Theor. Appl. Genet., 110, pp. 294-302Lee, M., Godshalk, E.B., Lamkey, K.R., Woodman, W.W., Association of restriction fragment length polymorphisms among maize inbreds with agronomic performance of their crosses (1989) Crop Sci., 29, pp. 1067-1071Lu, H., Bernardo, R., Molecular marker diversity among current and historical maize inbreds (2001) Theor. Appl. Genet., 103, pp. 613-617Malécot, G., (1948) Les Mathématiques de l'Hérédite, , Masson et Cie, ParisMantel, N., The detection of disease clustering and a generalized regression approach (1967) Cancer Res., 37, pp. 209-220Melchinger, A.E., Genetic diversity and heterosis (1999) The Genetics and Exploitation of Heterosis in Crops, pp. 99-118. , J.G. Coors, S. Pandey (Eds.), Am. Soc. Agron. Madison WIMenkir, A., Melake-Berhan, A., Ingelbrecht, A.T., Adepoju, A., Grouping of tropical mid-altitude maize inbred lines on the basis of yield data and molecular markers (2004) Theor. Appl. Genet., 108, pp. 1582-1590Mille, M., (1997) TFPGA: Toll for Population Genetic Analysis, Version 1.3, , Northern Arizona UniversityPejic, I., Ajmone Marsan, P., Morgante, M., Kozumplick, V., Castiglioni, P., Taramino, G., Motto, M., Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs, and AFLPs (1998) Theor. Appl. Genet., 97, pp. 1428-11255Reif, J.C., Melchinger, A.E., Frisch, M., Genetical and mathematical properties of similarity and dissimilarity coefficients applied in plant breeding and seed bank management (2005) Crop Sci., 45, pp. 1-7Reif, J.C., Melchinger, A.E., Xia, X.C., Warburton, M.L., Hoisington, D.A., Vasal, S.K., Srinivasan, G., Frisch, M., Genetic distance based on simple sequence repeats and heterosis in tropical maize populations (2003) Crop Sci., 43, pp. 1275-1282Reif, J.C., Melchinger, A.E., Xia, X.C., Warburton, M.L., Hoisington, D.A., Vasal, S.K., Beck, D., Frisch, M., Use of SSRs for establishing heterotic groups in subtropical maize (2003) Theor. Appl. Genet., 107, pp. 947-957Rohlf, F.J., (2000) NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System. Version 2.1, , Exeter Software. Setauket. NYSaghai-Maroof, M.A., Soliman, K.M., Jorgensen, R.A., Allard, R.W., Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosomal location, and population dynamics (1984) Proc. Natl. Acad. Sci. USA, 83, pp. 1757-1761(1998) SAS Language Guide for Personal Computers. Release 6.03 Ed., , SAS INSTITUTE, SAS Inst. Cary. NCSibov, S.T., Souza Jr., C.L., Garcia, A.A.F., Silva, A.R., Garcia, A.F., Mangolin, C.A., Benchimol, L.L., Souza, A.P., Molecular mapping in tropical maize (Zea mays L.) using microsattelite markers. 2. Quantitative trait loci (QTL) for grain yield, plant height, ear height and grain moisture (2003) Hereditas, 139, pp. 107-115Smith, O.S., Smith, J.S.C., Bowen, S.L., Tenborg, R.A., Wall, S.J., Similarities among a group of elite inbreds as measured by pedigree, F 1 heterosis, and RFLPs (1990) Theor. Appl. Genet., 80, pp. 833-840Steel, R.G.D., Torrie, J.H., (1980) Principles and Procedures of Statistics. 2. Ed., 633p. , New York: McGraw HillVaz Patto, M.C., Satovic, Z., Pêgo, S., Fevereiro, P., Assessing the genetic diversity of Portuguese maize germoplasm using microsatellite markers (2004) Euphytica, 137, pp. 63-72Xia, X.C., Reif, J.C., Hoisington, D.A., Melchinger, A.E., Frisch, M., Warburton, M.L., Genetic diversity among CIMMYT maize inbred lines investigated with SSR markers (2004) Crop Sci., 44, pp. 2230-2237Xu, S., Liu, J., Liu, G., The use of SSRs for predicting the hybrid yield and yield heterosis in 15 key inbred lines of Chinese maize (2004) Hereditas, 141, pp. 207-21

Chemical and Sensorial Characterization of Tropical Syrah Wines Produced at Different Altitudes in Northeast of Brazil

Over the years, viticulture has expanded to new regions outside the temperate zones, such as Northeast Brazil, India, Thailand, Myanmar, Vietnam, Bangladesh and Venezuela, characterized by the production of tropical wines. It is important for the productive sector to comprehend the effects of grapevine interaction with the characteristics of each new region on wines composition. In this study, the composition of wines of Syrah from two regions with different altitudes in Northeast Brazil were analyzed by different methodologies to characterize chemical compounds as sugar, acids, minerals, phenolics (anthocyanins, flavonols, stilbenes and condensed tannins) and the sensory profile. The wines of the Bahia region (1100 m of altitude) obtained high concentrations for chemical parameters related to color, monomeric anthocyanins, stilbenes and monomeric and oligomeric tannins. Wines of the low altitude region, Pernambuco (350 m of altitude) were characterized by higher concentrations of flavonols (kaempferol, isorhamnetin, quercetin and rutin) and polymerized tannins. The chemical composition of wines from the two studied regions was influenced by altitude. A trend towards higher concentrations in most for phenolic compounds analyzed was observed in wines from the higher altitude region during the two years of study. Regarding the sensory profile, fruity, floral, herbaceous and empyreumatic attributes aromatic obtained highest scores in wines of the 350 m altitude region, the other attributes were dependent on the year of harvest

Processing and characterization of glass-ceramic foams belonging to the Li2O-ZrO2-Al2O3-SiO2 (LZSA) system produced by gelcasting

In this work the viscosimetry technique was used to evaluate the rheological characteristics of ceramic suspensions prepared with a LZSA (Li2O-ZrO2-SiO2-Al2O3) glass. From the rheological characterization it was possible to establish optimized conditions of solid fraction, dispersant, organic monomers and foaming agent for the production of glass-ceramic foams by gelcasting. The resulting foams were subjected to heattreatments at 200-500ºC/60 min, for degradation of organics and at 950ºC/60 min for sintering and crystallization. With the obtained porous ceramic bodies linear thermal shrinkage, apparent density and mechanical strength measurements as well as microstructural analysis were performed. The adaptation of the rheological characteristics of the LZSA parent glass powder with the gelcasting processing technique allowed the production of ceramic foams with high open and interconnected porosity (>90%) with good thermal stability and with mechanical strength suitable for the production of porous ceramics.Neste trabalho, a técnica de viscosimetria foi utilizada para avaliar as características reológicas de suspensões preparadas com precursor vitrocerâmico do sistema LZSA (Li2O-ZrO2-SiO2-Al2O3). A partir da caracterização reológica, foi possível estabelecer condições composicionais adequadas de fração de sólidos, dispersante e monômeros orgânicos. A quantidade de agente espumante para produção de espumas vitrocerâmicas por gelcasting foi determinada pela variação volumétrica após agitação. As espumas resultantes foram submetidas a tratamentos térmicos no intervalo de temperatura compreendido entre 200 e 500ºC/60 min, para degradação da matéria orgânica e a 950ºC/60 min para sinterização e cristalização. Com os corpos cerâmicos porosos foram realizadas medidas de retração térmica, densidade aparente, análise microestrutural e resistência mecânica. A adequação das características reológicas do precursor LZSA por gelcasting permitiu a produção de espumas com elevada porosidade (> 90%) aberta e interconectada com boa estabilidade térmica e com resistência mecânica compatível com cerâmicas [email protected]@[email protected]@emc.ufsc.brUniversidade Federal de Santa Catarina Departamento de Engenharia Mecânica Programa de Pós-Graduação em Ciência e Engenharia de MateriaisCentro Universitário Barriga Verde - UNIBAVEUniversidade Federal de São Paulo (UNIFESP) Departamento de Ciência e TecnologiaUNIFESP, Depto. de Ciência e TecnologiaSciEL

Processing of tetrahedrite-based thermoelectric materials using tetrahedrite-tennantite copper ores

ABSTRACT: Tetrahedrite-based compounds are considered a promising thermoelectric material. The tetrahedrite-tennantite series, that can be generically expressed as Cu6[Cu4(Fe,Zn)2](Sb,As)4S13, is one of the most abundant sulfosalt minerals in the earth’s crust. The Iberian Pyrite Belt (IPB), located in southern Portugal and Spain, is the host of the largest concentration of massive sulfide deposits worldwide, where the tetrahedrite-tennantite series mineral is one of the constituents. In this work we evaluate the effectiveness of combining synthetic tetrahedrite (ST) with tetrahedritetennantite ore samples (TTOS) in the mechanochemical synthesis (MCS) process of tetrahedrite based compounds. The ore samples were collected from two distinct deposits within the Portuguese part of the IPB, the Neves Corvo mine and the abandoned Barrigão copper mine. The ST, also produced by MCS, were mixed with the TTOS in different mass ratios ranging from 20-80%. The influence of the chemical composition and phase constitution of the ore samples and of the mixing ratios will be presented in relation to the micro-structural properties of the obtained materials. The results here described are part of a broader study dedicated to the development of energy-harvesting applications based on tetrahedrite.info:eu-repo/semantics/publishedVersio

Bio-based synthesis of oxidation resistant copper nanowires using an aqueous plant extract

Copper nanowires have recently emerged as promising nanomaterials for transparent conducting electrodes applications, however, their production commonly involves the use of harmful reagents. In this study, we describe for the first time a simple and cost-effective bio-based synthesis of copper nanowires using an aqueous plant extract (Eucalyptus globulus) as the reducing/stabilizing agent and oleic acid and oleylamine as surfactants. Well-dispersed crystalline copper nanowires (λmáx = 584–613 nm) were obtained with average diameters in the nanometric range (44 and 145 nm) and lengths in the micrometric range (from 5 to dozens of micrometres) using extract concentrations between 10 and 50 mg mL−1. Moreover, the aspect ratio of these nanowires can be adjusted (from around 14–20 to 160–400) by changing the experimental conditions, namely the use of oleic acid. Phenolic compounds were found to have a key role in this bioreduction process allowing to obtain practically only nanowires (without other morphologies). Nevertheless, the use of oleic acid/oleylamine is essential to manipulate their size and aspect ratio. Most importantly, these bio-based copper nanowires were found to be resistant under storage in ethanol and when submitted to air exposure, both for 2 weeks, certainly due to the adsorption of antioxidant biomolecules (phenolic) at their surface, thus avoiding the use of other polymeric protective layers. The conductivity of the CuNWs was found to be 0.009 S cm−1. As a result, this study opens a new standpoint in this field, “closing the door” to the use of hazardous reagents and synthetic polymeric protective layers, on the production of stable copper nanowires with potential application as conductive materials.publishe

Efeito de formulações na absorção e translocação do glyphosate em soja transgênica

Este trabalho teve como objetivo avaliar a absorção e translocação de glyphosate em diferentes formulações por plantas de soja (variedade CD 219RR). Para isso, aplicou-se o 14C-glyphosate misturado à calda em três formulações comerciais (Roundup Ready® e R. Transorb®, ambas contendo o sal de isopropilamina, e Zapp Qi®, formulado à base do sal potássico), quando as plantas apresentavam o segundo trifólio completamente expandido. Transcorridas 4, 16, 40 e 64 horas após a aplicação, as plantas foram coletadas e fracionadas, separando-se a folha de aplicação (trifólio), a parte aérea, as raízes e os nódulos radiculares. O 14C-glyphosate não-absorvido foi recuperado e contado por meio da lavagem da folha (metanol 80%). Entre as formulações foi observada variação na penetração e na translocação do 14C-glyphosate para as diferentes partes avaliadas. Todavia, em todas as formulações a maior absorção se deu nos intervalos posteriores a 16 horas da aplicação. Em relação ao total de herbicida encontrado nas plantas de soja, maior percentual na parte aérea foi observado quando se aplicou o Zapp Qi® (sal potássico) e, nas raízes, o R. Transorb® (sal de isopropilamina). Detectou-se a presença de 14C glyphosate nos nódulos radiculares das plantas em todos os tratamentos, sendo o maior percentual observado quando se utilizou R. Transorb®, 40 horas após a aplicação (0,13% do total medido ou 0,4% considerando somente o total presente na planta). Os resultados reforçam a hipótese de que o glyphosate pode prejudicar a simbiose entre rizóbio e soja, uma vez que o microssimbionte também apresenta em seu metabolismo a EPSPS, sensível a esse herbicida.This study was carried out to evaluate the absorption and translocation of glyphosate formulations in genetically modified (GM) soybean by applying 14C-glyphosate mixed to three glyphosate formulations (Roundup Ready® and R. Transorb® - both with isopropylamine salt, and Zapp Qi®, formulated from potassic salt ), using a precision micro syringe. Plant samples were collected after herbicide application (4, 16, 40 and 64 hours) and then divided into leaf (trifolium), aerial part, roots and root nodes for radiation reading. 14C-glyphosate that was not absorbed was recovered and counted by washing the leaf with methanol. Penetration and translocation of 14C-glyphosate to the different parts evaluated was found to vary. However, the highest absorption was verified at intervals after 16 hours of application. The highest herbicide percentage in the aerial part of the soybean plants was found when Zapp (potassic salt) was applied on the aerial part and when isopropylamin salt was applied on the roots; 14C-glyphosate was found in the plant root nodules in all treatments, with the highest percentage being observed with R. Transorb®, 40 hours after application (0.13% of the total measured or 0.4%, considering only the plant total). Results highlight the hypothesis that glyphosate could harm symbiosis between rhizobium and soybean, since the former also shows in its metabolism EPSPS, which is susceptible to this herbicide

High prevalence of HPV 18 and multiple infections with oncogenic HPV genotypes in women at risk of cervical cancer examined in Manaus, Brazil

Cervical cancer is a serious public health problem in Brazil, especially in Manaus (Amazonas), the city with the highest incidence rate of cervical cancer in the country. Persistent infection with oncogenic human papillomavirus (HPV) genotypes is the cause of disease development. The aim of this study was to investigate the prevalence of oncogenic genotypes in women at high risk for cervical precancer examined in two policlinics in Manaus. One hundred and two patients who underwent colposcopy took part in the research. The DNA samples obtained from the cervical epithelium were analyzed by PCR with type-specific primers for the detection of eight oncogenic genotypes, which were chosen based on previous studies. The presence of HPV virus was detected in all samples. The most prevalent oncogenic genotypes were 18 (47.1%) and 16 (45.1%). Interestingly, HPV 18 was considered uncommon in this region. In addition to these, genotypes 31 (19.6%), 58 (19.6%), 33 (18.6%), and 45 (15.7%) also had a relatively high frequency in this population. Fifty-six women (54.9%) had multiple infections with up to five oncogenic types. Also, the presence of genotypes other than 16 and 18 was observed in most samples (57.8%), which also deserves attention since they are not covered by currently available vaccines against HPV in Brazil. The high prevalence and multiple infections with several oncogenic HPV genotypes in association with precursor lesions for cervical cancer highlighted the need to improve strategies to prevent this disease in Amazonas