Oxygen surface exchange and diffusion in fast ionic conductors

The rate of oxygen surface exchange on selected bulk oxides exhibiting enhanced oxygen ion conductivity has been derived by measuring 18O penetration profiles using a high sensitivity dynamic SIMS technique. These values for the oxygen surface exchange coefficient (K) were used to derive the steady-state oxygen fluxes through the oxide surface for conditions when the bulk oxide was in equilibrium with 1 atm. of oxygen at 500 and 700°C. The oxygen fluxes were transformed into current fluxes and compared with available exchange current densities (iO) measured using electrochemical techniques. The two sets of current densities exhibited large differences for zirconia based electrolytes which confirmed the important role of platinum as an electro-catalyst. However for bismuth based electrolytes good agreement was noted between the two sets of iO values. It was concluded therefore that the dissociative adsorption of oxygen occurred predominately on the surface of the Bi2O3 based electrolyte and that the presence of a metal electrode (Pt or Au) had little effect upon the overall exchange current kinetics

SSR assessment of Phytophthora infestans populations on tomato and potato in British gardens demonstrates high diversity but no evidence for host specialisation

Phytophthora infestans populations can differ in composition as a result of host-specialisation on tomato and potato hosts. In Great Britain many amateur gardeners grow outdoor tomatoes but there is little or no commercial tomato production outdoors. This study analysed isolates of P. infestans from British gardens with twelve multiplexed SSR markers that are used to monitor the disease on commercial potato crops. Samples of P. infestans from tomato hosts were collected in three years and from potato in one year from across Great Britain. Seven previously unreported genotypes were detected in garden populations and higher frequencies of unique clonal lineages (28% to 40%) were present compared with populations from British commercial potato crops reported elsewhere. Garden populations had a lower proportion (11% to 48% less) of the most common lineages (13_A2 and 6_A1) which together made up at least 86% of the commercial potato populations during the sampling period. Host species accounted for only 2.6% of molecular variance detected between garden potato- and tomato-hosted samples. No significant difference in clonal lineage composition was found between host species in Great Britain and this could be due to the whole P. infestans population over-wintering on potato. British garden populations on both hosts were much more diverse than those on commercial potato crops; this finding may be influenced by less frequent fungicide use by gardeners and a higher diversity of un-sprayed susceptible potato cultivars enabling metalaxyl-sensitive and less virulent genotypes to survive in gardens

Genesis of ancestral haplotypes: RNA modifications and reverse transcription–mediated polymorphisms

Understanding the genesis of the block haplotype structure of the genome is a major challenge. With the completion of the sequencing of the Human Genome and the initiation of the HapMap project the concept that the chromosomes of the mammalian genome are a mosaic, or patchwork, of conserved extended block haplotype sequences is now accepted by the mainstream genomics research community. Ancestral Haplotypes (AHs) can be viewed as a recombined string of smaller Polymorphic Frozen Blocks (PFBs). How have such variant extended DNA sequence tracts emerged in evolution? Here the relevant literature on the problem is reviewed from various fields of molecular and cell biology particularly molecular immunology and comparative and functional genomics. Based on our synthesis we then advance a testable molecular and cellular model. A critical part of the analysis concerns the origin of the strand biased mutation signatures in the transcribed regions of the human and higher primate genome, A-to-G versus T-to-C (ratio ~1.5 fold) and C-to-T versus G-to-A (≥1.5 fold). A comparison and evaluation of the current state of the fields of immunoglobulin Somatic Hypermutation (SHM) and Transcription-Coupled DNA Repair focused on how mutations in newly synthesized RNA might be copied back to DNA thus accounting for some of the genome-wide strand biases (e.g., the A-to-G vs T-to-C component of the strand biased spectrum). We hypothesize that the genesis of PFBs and extended AHs occurs during mutagenic episodes in evolution (e.g., retroviral infections) and that many of the critical DNA sequence diversifying events occur first at the RNA level, e.g., recombination between RNA strings resulting in tandem and dispersed RNA duplications (retroduplications), RNA mutations via adenosine-to-inosine pre-mRNA editing events as well as error prone RNA synthesis. These are then copied back into DNA by a cellular reverse transcription process (also likely to be error-prone) that we have called "reverse transcription-mediated long DNA conversion." Finally we suggest that all these activities and others can be envisaged as being brought physically under the umbrella of special sites in the nucleus involved in transcription known as "transcription factories."

A Non-Perturbative Chiral Approach for Meson-Meson Interactions

A non-perturbative method which combines constraints from chiral symmetry breaking and coupled channel unitarity is used to describe meson-meson interactions up to \sqrt{s}\lesssim 1.2 GeV, extending in this way the range of applicability of the information contained in Chiral Perturbation Theory (\chi PT), since this perturbative series is typically restricted to \sqrt{s}\lesssim 500 MeV. The approach uses the O(p^2) and O(p^4) \chiPT Lagrangians. The seven free parameters resulting from the O(p^4) Lagrangian are fitted to the experimental data. The approach makes use of the expansion of T^{-1} instead of the amplitude itself as done in \chiPT. The former expansion is suggested by analogy with the effective range approximation in Quantum Mechanics and it appears to be very useful. The results, in fact, are in good agreement with a vast amount of experimental analyses.Comment: 4 pages, 2 figures, LaTeX, Talk given at PANIC99, Uppsala (Sweden), June 10-16, 199

Virus and prokaryote enumeration from planktonic aquatic environments by epifluorescence microscopy with SYBR Green I

Viruses are the most abundant biological entities in aquatic environments, typically exceeding the abundance of bacteria by an order of magnitude. The reliable enumeration of virus-like particles in marine microbiological investigations is a key measurement parameter. Although the size of typical marine viruses (20-200 nm) is too small to permit the resolution of details by light microscopy, such viruses can be visualized by epifluorescence microscopy if stained brightly. This can be achieved using the sensitive DNA dye SYBR Green I (Molecular Probes-Invitrogen). The method relies on simple vacuum filtration to capture viruses on a 0.02-μm aluminum oxide filter, and subsequent staining and mounting to prepare slides. Virus-like particles are brightly stained and easily observed for enumeration, and prokaryotic cells can easily be counted on the same slides. The protocol provides an inexpensive, rapid (30 min) and reliable technique for obtaining counts of viruses and prokaryotes simultaneously

Thin-Film Metamaterials called Sculptured Thin Films

Morphology and performance are conjointed attributes of metamaterials, of which sculptured thin films (STFs) are examples. STFs are assemblies of nanowires that can be fabricated from many different materials, typically via physical vapor deposition onto rotating substrates. The curvilinear--nanowire morphology of STFs is determined by the substrate motions during fabrication. The optical properties, especially, can be tailored by varying the morphology of STFs. In many cases prototype devices have been fabricated for various optical, thermal, chemical, and biological applications.Comment: to be published in Proc. ICTP School on Metamaterials (Augsut 2009, Sibiu, Romania

Managing phase purities and crystal orientation for high-performance and photostable cesium lead halide perovskite solar cells

Inorganic perovskites with cesium (Cs+) as the cation have great potential as photovoltaic materials if their phase purity and stability can be addressed. Herein, a series of inorganic perovskites is studied, and it is found that the power conversion efficiency of solar cells with compositions CsPbI1.8Br1.2, CsPbI2.0Br1.0, and CsPbI2.2Br0.8 exhibits a high dependence on the initial annealing step that is found to significantly affect the crystallization and texture behavior of the final perovskite film. At its optimized annealing temperature, CsPbI1.8Br1.2 exhibits a pure orthorhombic phase and only one crystal orientation of the (110) plane. Consequently, this allows for the best efficiency of up to 14.6% and the longest operational lifetime, T S80, of ≈300 h, averaged of over six solar cells, during the maximum power point tracking measurement under continuous light illumination and nitrogen atmosphere. This work provides essential progress on the enhancement of photovoltaic performance and stability of CsPbI3 − x Brx perovskite solar cells

Quantification of pathogens and markers of fecal contamination during storm events along popular surfing beaches in San Diego, California

Along southern California beaches, the concentrations of fecal indicator bacteria (FIB) used to quantify the potential presence of fecal contamination in coastal recreational waters have been previously documented to be higher during wet weather conditions (typically winter or spring) than those observed during summer dry weather conditions. FIB are used for management of recreational waters because measurement of the bacterial and viral pathogens that are the potential causes of illness in beachgoers exposed to stormwater can be expensive, time-consuming, and technically difficult. Here, we use droplet digital Polymerase Chain Reaction (digital PCR) and digital reverse transcriptase PCR (digital RT-PCR) assays for direct quantification of pathogenic viruses, pathogenic bacteria, and source-specific markers of fecal contamination in the stormwater discharges. We applied these assays across multiple storm events from two different watersheds that discharge to popular surfing beaches in San Diego, CA. Stormwater discharges had higher FIB concentrations as compared to proximal beaches, often by ten-fold or more during wet weather. Multiple lines of evidence indicated that the stormwater discharges contained human fecal contamination, despite the presence of separate storm sewer and sanitary sewer systems in both watersheds. Human fecal source markers (up to 100% of samples, 20-12440 HF183 copies per 100 ml) and human norovirus (up to 96% of samples, 25-495 NoV copies per 100 ml) were routinely detected in stormwater discharge samples. Potential bacterial pathogens were also detected and quantified: Campylobacter spp. (up to 100% of samples, 16-504 gene copies per 100 ml) and Salmonella (up to 25% of samples, 6-86 gene copies per 100 ml). Other viral human pathogens were also measured, but occurred at generally lower concentrations: adenovirus (detected in up to 22% of samples, 14-41 AdV copies per 100 ml); no enterovirus was detected in any stormwater discharge sample. Higher concentrations of avian source markers were noted in the stormwater discharge located immediately downstream of a large bird sanctuary along with increased Campylobacter concentrations and notably different Campylobacter species composition than the watershed that had no bird sanctuary. This study is one of the few to directly measure an array of important bacterial and viral pathogens in stormwater discharges to recreational beaches, and provides context for stormwater-based management of beaches during high risk wet-weather periods. Furthermore, the combination of culture-based and digital PCR-derived data is demonstrated to be valuable for assessing hydrographic relationships, considering delivery mechanisms, and providing foundational exposure information for risk assessment

Surface effects of vapour-liquid-solid driven Bi surface droplets formed during molecular-beam-epitaxy of GaAsBi

Herein we investigate a (001)-oriented GaAs1−xBix/GaAs structure possessing Bi surface droplets capable of catalysing the formation of nanostructures during Bi-rich growth, through the vapourliquid-solid mechanism. Specifically, self-aligned “nanotracks” are found to exist trailing the Bi droplets on the sample surface. Through cross-sectional high-resolution transmission electron microscopy the nanotracks are revealed to in fact be elevated above surface by the formation of a subsurface planar nanowire, a structure initiated mid-way through the molecular-beam-epitaxy growth and embedded into the epilayer, via epitaxial overgrowth. Electron microscopy studies also yield the morphological, structural, and chemical properties of the nanostructures. Through a combination of Bi determination methods the compositional profile of the film is shown to be graded and inhomogeneous. Furthermore, the coherent and pure zincblende phase property of the film is detailed. Optical characterisation of features on the sample surface is carried out using polarised micro-Raman and micro-photoluminescence spectroscopies. The important light producing properties of the surface nanostructures are investigated through pump intensity-dependent micro PL measurements, whereby relatively large local inhomogeneities are revealed to exist on the epitaxial surface for important optical parameters. We conclude that such surface effects must be considered when designing and fabricating optical devices based on GaAsBi alloys

Photon and dilepton emission rates from high density quark matter

We compute the rates of real and virtual photon (dilepton) emission from dense QCD matter in the color-flavor locked (CFL) phase, focusing on results at moderate densities (3-5 times the nuclear saturation density) and temperatures $T\simeq80$ MeV. We pursue two approaches to evaluate the electromagnetic (e.m.) response of the CFL ground state: (i) a direct evaluation of the photon self energy using quark particle/-hole degrees of freedom, and (ii) a Hidden Local Symmetry (HLS) framework based on generalized mesonic excitations where the $\rho$ meson is introduced as a gauge boson of a local SU(3) color-flavor group. The $\rho$ coupling to generalized two-pion states induces a finite width and allows to address the issue of vector meson dominance (VMD) in the CFL phase. We compare the calculated emissivities (dilepton rates) to those arising from standard hadronic approaches including in-medium effects. For rather large superconducting gaps (several tens of MeV at moderate densities), as suggested by both perturbative and nonperturbative estimates, the dilepton rates from CFL quark matter turn out to be very similar to those obtained in hadronic many-body calculations, especially for invariant masses above $M\simeq0.3$ GeV. A similar observation holds for (real) photon production.Comment: 18 pages, 12 figure