Quality Of Drinking Water Fluoridation Of Capão Bonito, S P, Brazil, Evaluated By Operational And External Controls

Purpose: To evaluate the quality of drinking water fluoridation of Capão Bonito, S P, Brazil, whose optimal fluoride concentration should be between 0.6 to 0.8 ppm F, considering the balance benefits/risks. Methods: Historical records (n=1,964) from 2005 to 2009 of the water treatment plant (operational control) were evaluated. Also, from July 2009 to June 2010, 120 samples of the network water were collected for analysis and the fluoride concentrations found (external control) were compared with records of operational control of the same period. Results: According to the historical records, 76.3% of the samples had acceptable fluoride concentration and this value was confirmed by the external control done during one year, which found that 80.8% of samples were within the optimal range. However, considering the samples out the optimal range, while the records of the operational control showed values below the minimum, the results of the external control found higher percentage of samples above the maximum. Conclusion: The data show the relevance to have a quality control of drinking water fluoridation because at same time the operational control analysis suggests that certain percentage of the population would not be receiving anticaries benefits, the external control indicates that it would be in increased risk of fluorosis. Copyright: © 2011 Olivati et al.;.264285290McDonagh, M.S., Whiting, P.F., Wilson, P.M., Sutton, A.J., Chestnutt, I., Cooper, J.X., Systematic review of water fluoridation (2000) BMJ, 321, pp. 855-859Galagan, D.J., Vermillion, J.R., Determining optimum fluoride concentrations (1957) Public Health Rep, 72, pp. 491-493Frazão, P., Peres, M.A., Cury, J.A., Drinking water quality and fluoride concentration (2011) Rev Saude Publica, 45, pp. 964-973Brasil. Lei Federal n°6.050, de 24/05/1974 (1974) Coleção Das Leis de 1974: Atos do Poder Legislativo: Leis de Abril a Junho, 3, p. 107. , Brasília, DF: Departamento de Imprensa NacionalCatani, D.B., Amaral, R.C., Oliveira, C., Souza, M.L.R., Cury, J.A., Ten years of external control of the fluoride level in public drinking water by Brazilian cities, Brazil, 1996-2006 (in Portuguese) (2008) RGO x, 56, pp. 145-149Panizzi, M., Peres, M.A., Ten years of external control over water fluoridation in Chapecó, Santa Catarina State, Brazil (in Portuguese) (2008) Cad Saúde Pública, 24, pp. 2021-2031Portaria 635 de 26 de Dezembro de 1975. Aprova Normas e Padrões Sobre a Fluoretação da Água de Sistemas Públicos de Abastecimento. Diario Oficial União, , http://189.28.128.100/dab/docs/legislacao/portaria635_26_12_75.pdf, 30 jan. 1976 cited 2006 Jan. 18(2006) National Guideline on Sampling Plan of the Environment Health Vigilance for Assessment of Quality of Water for Human use, , Brazil. Ministry of Health, Brasília, in PortugueseMartínez-Mier, E.A., Cury, J.A., Heilman, J.R., Katz, B.P., Levy, S.M., Li, Y., Maguire, A., Development of gold standard ion-selective electrode-based methods for fluoride analysis (2011) Caries Res, 45, pp. 3-12Moura, M.S., Silva, J.S., Simplício, A.H.M., Cury, J.A., Long-Term Assessment of Fuoride Level of Public Water Supply in Teresina-Piauí (in Portuguese) (2005) Rev Odonto Ciênc, 20, pp. 132-136Ramires, I., Maia, L.P., Rigolizzo, D.S., Lauris, J.L.P., Buzalaf, M.A.R., External control of water supply in Bauru, S P, Brazil (2006) Rev Saúde Pública, 40, pp. 883-889Amaral, R.C., Sousa, M.L.R., Eight months of external control of fluoride levels in the public water supply in Piracicaba, São Paulo, Brazil (2007) Revista de Odontologia da Universidade Cidade de São Paulo (in Portuguese), 19, pp. 131-136Cury, J.A., Tenuta, L.M., Enamel remineralization: Controlling the caries disease or treating early caries lesions? (2009) Braz Oral Res, 23 (1 SUPPL.), pp. 23-30Bardsen, A., "Risk periods" associated with the development of dental fluorosis in maxillary permanent central incisors: A meta-analysis (1999) Acta Odontol Scand, 57, pp. 247-256Catani, D.B., Hugo, F.N., Cypriano, S., Sousa Mda, L., Cury, J.A., Relationship between fluoride levels in the public water supply and dental fluorosis (2007) Rev Saude Publica, 41, pp. 732-739Catani, D.B., Tenuta, L.M., Andaló, F.A., Cury, J.A., Fluorosis in rats exposed to oscillating chronic fluoride doses (2010) Braz Dent J, 21, pp. 32-37Documento de Consenso Técnico, , http://www.cecol.fsp.usp.br/dcms/fck/file/SeminarioVigifluor2011/ CECOL-USP-ClassificacaoAguasSegundoTeorFluor-DocumentoConsensoTecnico-2011(2). pdf, Classificação de águas de abastecimento público, Acesso em Nov. 1

3D kinematics of the near-IR HH 223 outflow in L723

In this work, we derive the full 3D kinematics of the near-infrared outflow HH 223, located in the dark cloud Lynds 723 (L723), where a well-defined quadrupolar CO outflow is found. HH 223 appears projected on to the two lobes of the eastwest CO outflow. The radio continuum source VLA 2, towards the centre of the CO outflow, harbours a multiple system of low-mass young stellar objects. One of the components has been proposed to be the exciting source of the eastwest CO outflow. From the analysis of the kinematics, we get further evidence on the relationship between the near-infrared and CO outflows and on the location of their exciting source. The proper motions were derived using multi-epoch, narrow-band H2 (2.122 μm line) images. Radial velocities were derived from the 2.122 μm line of the spectra. Because of the extended (∼5 arcmin), S-shaped morphology of the target, the spectra were obtained with the multi-object-spectroscopy (MOS) observing mode using the instrument Long-Slit Intermediate Resolution Infrared Spectrograph (LIRIS) at the 4.2 m William Herschel Telescope. To our knowledge, this work is the first time that MOS observing mode has been successfully used in the near-infrared range for an extended target

Efectividad de un programa de intervención contra el ciberbullying

Antecedentes . La inmersión de las TICs e internet en la vida de los jóvenes y los riesgos que de su uso se derivan ponen de manifiesto la necesidad de alfabetizar digitalmente a los adolescentes. El programa ConRed, dirigido a docentes, alumnos y familias, ha conseguido que entre estudiantes se reduzcan problemas como el ciberbullying, la dependencia virtual y el escaso control sobre la información personal publicada en Internet. Este artículo examina el impacto de una adaptación del Proyecto ConRed tras eliminar su ca rácter ecológico y reducir el número de sesiones con el alumnado. Método . Se ha utilizado una metodología cuasi experimental y una muestra de 355 estudiantes entre grupo experimental (N=163) y control (N=192) (51,7%♀; Edad M= 14,6; DT= 1,7), quienes cumpli mentaron en pretest y postest cuestionarios sobre ciberbullying, percepción del control de la información y dependencia a internet. Resultados . Los análisis de ANOVA de medidas repetidas solo reflejaron resultados favorables para la prevención e intervención de bulling. Conclusiones. Este trabajo constata la necesidad de intervenciones anti-ciberbullying donde participe alumnado, familias y profesorado y refuerza la evidencia científica de que los programas destinados en reducir la violencia en la red influyen irremediablemente en los agresores de violencia tradicional.Background . ICT Immersion and the internet in the youngs lives and the risks arising from its use highlight the need for digital literacy to adolescents. The ConRed program, aimed at teachers, students and families, has made problems like cyberbullying, the virtual dependency and poor control over personal information on the Internet among students to be reduced. This article examines the impact of an ConRed proyect adaptation after removing their ecological character and reduce the number of sessions with the students. Method . We used a quasi-experimental methodology and a sample of 355 students in the experimental group (N = 163) and control (N = 192) (51.7% ♀, age M = 14.6, SD = 1.7) who completed pretest and posttest questionnaires about cyberbullying, control of information perception and internet dependency. Results. Analysis of repeated ANOVA measures only reflected favorable for the prevention and intervention of traditional bullying results. Conclusions . This work shows the necessity of anti-cyberbullying interventions where students, families and teachers participates and reinforces the scientific evidence that programs aimed at reducing violence in the network inevitably affect traditional violence offenders

Interplay between pairing and exchange in small metallic dots

We study the effects of the mesoscopic fluctuations on the competition between exchange and pairing interactions in ultrasmall metallic dots when the mean level spacing is comparable or larger than the BCS pairing energy. Due to mesoscopic fluctuations, the probability to have a non-zero spin ground state may be non-vanishing and shows universal features related to both level statistics and interaction. Sample to sample fluctuations of the renormalized pairing are enlightened.Comment: 10 pages, 5 figure

Downregulation of NAC transcription factors modifies cell wall composition and increases strawberry fruit firmness

The strawberry is a soft fruit with a very short post-harvest shelf life. The changes in texture during fruit ripening are mainly due to the dissolution of the middle lamellae, reducing cell-to-cell adhesion, and the weakening of parenchymal cell walls as result of the action of cell wall modifying enzymes. At present, no master regulator of this process has been discovered yet. NAC transcription factors have been involved in numerous physiological processes, including fruit ripening. In strawberry, the NAC family comprises more than 110 genes, and at least 6 of them are expressed during fruit development. In this research, we performed a functional analysis of two ripening-related NAC genes, FaNAC2 and FaNAC3, in Fragaria x ananassa Duch. cv. Chandler. Several RNAi transgenic lines showing low FaNAC2 or FaNAC3 mRNA levels in fruit were obtained through Agrobacterium-mediated transformation. These lines produced fruits significantly firmer than control at the ripe stage, being the increase in firmness higher in FaNAC2 silenced plants. Cell walls were extracted from ripe transgenic fruits and characterized by ELISA and Epitope Detection Chromatography (EDC), using monoclonal antibodies against different polysaccharide epitopes. FaNAC2 transgenic lines showed more extensive changes than FaNAC3; these modifications involved increased amounts of demethylated pectins (LM19) in water and CDTA fractions and an alteration of the lateral branches of RG-I, decreasing the amount of arabinan epitopes and increasing galactan epitopes detected by LM6 and LM5, respectively. The amount of arabinogalactan proteins recognized by the JIM13 antibody was also affected, decreasing in the Na2CO3 fraction and increasing in the 4M KOH and cellulase fraction of the transgenic lines.The results obtained indicate that NAC genes could be involved in the regulation of cell wall disassembly associated to strawberry fruit softening.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Matrix metalloproteinase-10 effectively reduces infarct size in experimental stroke by enhancing fibrinolysis via a thrombin-activatable fibrinolysis inhibitor-mediated mechanism

BACKGROUND: The fibrinolytic and matrix metalloproteinase (MMP) systems cooperate in thrombus dissolution and extracellular matrix proteolysis. The plasminogen/plasmin system activates MMPs, and some MMPs have been involved in the dissolution of fibrin by targeting fibrin(ogen) directly or by collaborating with plasmin. MMP-10 has been implicated in inflammatory/thrombotic processes and vascular integrity, but whether MMP-10 could have a profibrinolytic effect and represent a promising thrombolytic agent is unknown. METHODS AND RESULTS: The effect of MMP-10 on fibrinolysis was studied in vitro and in vivo, in MMP-10-null mice (Mmp10(-/-)), with the use of 2 different murine models of arterial thrombosis: laser-induced carotid injury and ischemic stroke. In vitro, we showed that MMP-10 was capable of enhancing tissue plasminogen activator-induced fibrinolysis via a thrombin-activatable fibrinolysis inhibitor inactivation-mediated mechanism. In vivo, delayed fibrinolysis observed after photochemical carotid injury in Mmp10(-/-) mice was reversed by active recombinant human MMP-10. In a thrombin-induced stroke model, the reperfusion and the infarct size in sham or tissue plasminogen activator-treated animals were severely impaired in Mmp10(-/-) mice. In this model, administration of active MMP-10 to wild-type animals significantly reduced blood reperfusion time and infarct size to the same extent as tissue plasminogen activator and was associated with shorter bleeding time and no intracranial hemorrhage. This effect was not observed in thrombin-activatable fibrinolysis inhibitor-deficient mice, suggesting thrombin-activatable fibrinolysis inhibitor inactivation as one of the mechanisms involved in the MMP-10 profibrinolytic effect. CONCLUSIONS: A novel profibrinolytic role for MMP-10 in experimental ischemic stroke is described, opening new pathways for innovative fibrinolytic strategies in arterial thrombosis

Experiencia clínica del tratamiento con onabotulinumtoxin A en pacientes con migraña refractaria

To analyse our experience in the treatment of refractory chronic migraine, episodic frequent refractory migraine (>= 10 days/month), with onabotulinumtoxin A (OnabotA). PATIENTS AND METHODS. Retrospective analysis of patients with refractory migraine who underwent, at least two sessions of OnabotA pericranial injections following the PREEMPT protocol between 2008 and 2012. The efficacy of OnabotA was evaluated comparing the basal situation with 12-16 weeks after the second session. We analysed the subjective improvement of the patients, number of days with headache, preventive and abortive drugs consumption, and adverse effects. RESULTS. Forty-one patients (37 women, 4 male) were identified. 65.8% patients experienced subjective improvement after OnabotA treatment. 36.58% responded (reduction of > 50% in headache days). Differences between days with headache before the first session (24.5 +/- 7.3), and 12-16 weeks after the second session (17.4 +/- 11.6), as well as the differences between the number of abortive drugs taken before the first session (26.8 +/- 23.1) and 12-16 weeks after the second session (16.7 +/- 19.3), were statistically significant (p < 0.001). Subgroups analysis showed that all differences were significant, except for the reduction of the number of days with headache in patients with episodic frequent refractory migraine. CONCLUSION. Our work shows that treatment with OnabotA is safe and useful in patients with episodic and chronic refractory migraine, including those patients with medication overuse headache

Usability Challenges in Smartphone Web Access: A Systematic Literature Review

Part 8: International Workshop on Information Engineering and ManagementInternational audienceSystematic literature reviews facilitate methodical understanding of current advances in a field. With the increasing popularity of smartphones, they have become an important means to access the web. Although the literature on this topic is growing in recent times, there has been no effort yet to systematically review it. This paper reports on a systematic literature review of primary studies from 2007 to 2012 that concern mobile web usability. We identify the usability dimensions tested and the testing procedures adopted in the literature. We anticipate that our work will not only help researchers understand the current state of usability testing of mobile web but also identify the areas where further research is needed in addressing the challenges identified

Cell wall disassembly is delayed by rhamnogalacturonate lyase gene silencing: potential role in fruit firmness

Strawberry fruits greatly reduce their quality due to softening during ripening with economically important losses. Texture changes of fleshy fruits during ripening are mainly due to middle lamellae dissolution, cell-to-cell adhesion losses and wall weakening of parenchyma cells by the coordinated action of several cell wall enzymes. Pectin degradation has been proven a key factor in strawberry softening by functional analysis of several pectinase genes (polygalacturonase, pectate lyase and -galactosidase). The complexity and highly dynamic nature of pectins remains a challenge to fully elucidate structure-function relationships of pectins. In this work, we present the functional analysis of two independent strawberry transgenic lines with more than 95% silencing of a rhamnogalacturonate lyase gene (FaRGLyase1). Firmness of ripe fruit was significantly higher in both transgenic lines than in the control. Cell walls from these fruits were extracted and analyzed by glycan microarray profiling. This high‐throughput technique allows a wide screening of cell-wall glycan occurrence based on the detection of specific cell wall oligosaccharide epitopes by monoclonal antibodies and reveals profiles which can be used as potential fingerprints specific for a singular organ and/or developmental stage. Our microarray results showed that the silencing of FaRGLyase1 reduced degradation of several rhamnogalacturonan-I related epitopes, as expected. Additionally, comparison of transgenic cell walls from ripe fruits with those extracted from control fruits at different developmental stages (green, white and red) by hierarchical clustering, demonstrated a higher similarity of transgenic fruit cell walls with the control cell walls from fruits at the white stage. Glycan microarray profiles revealed less degraded fruit cell walls as result of FaRGLyase1 down-regulation which could contribute to the increased firmness of transgenic fruitsUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech