25 research outputs found
Structural evolution drives diversification of the large LRR-RLK gene family
Cells are continuously exposed to chemical signals that they must discriminate between and respond to appropriately. In embryophytes, the leucineârich repeat receptorâlike kinases (LRRâRLKs) are signal receptors critical in development and defense. LRRâRLKs have diversified to hundreds of genes in many plant genomes. Although intensively studied, a wellâresolved LRRâRLK gene tree has remained elusive. To resolve the LRRâRLK gene tree, we developed an improved gene discovery method based on iterative hidden Markov model searching and phylogenetic inference. We used this method to infer complete gene trees for each of the LRRâRLK subclades and reconstructed the deepest nodes of the full gene family. We discovered that the LRRâRLK gene family is even larger than previously thought, and that protein domain gains and losses are prevalent. These structural modifications, some of which likely predate embryophyte diversification, led to misclassification of some LRRâRLK variants as members of other gene families. Our work corrects this misclassification. Our results reveal ongoing structural evolution generating novel LRRâRLK genes. These new genes are raw material for the diversification of signaling in development and defense. Our methods also enable phylogenetic reconstruction in any large gene family
Phylogeny.fr: robust phylogenetic analysis for the non-specialist
Phylogenetic analyses are central to many research areas in biology and typically involve the identification of homologous sequences, their multiple alignment, the phylogenetic reconstruction and the graphical representation of the inferred tree. The Phylogeny.fr platform transparently chains programs to automatically perform these tasks. It is primarily designed for biologists with no experience in phylogeny, but can also meet the needs of specialists; the first ones will find up-to-date tools chained in a phylogeny pipeline to analyze their data in a simple and robust way, while the specialists will be able to easily build and run sophisticated analyses. Phylogeny.fr offers three main modes. The âOne Clickâ mode targets non-specialists and provides a ready-to-use pipeline chaining programs with recognized accuracy and speed: MUSCLE for multiple alignment, PhyML for tree building, and TreeDyn for tree rendering. All parameters are set up to suit most studies, and users only have to provide their input sequences to obtain a ready-to-print tree. The âAdvancedâ mode uses the same pipeline but allows the parameters of each program to be customized by users. The âA la Carteâ mode offers more flexibility and sophistication, as users can build their own pipeline by selecting and setting up the required steps from a large choice of tools to suit their specific needs. Prior to phylogenetic analysis, users can also collect neighbors of a query sequence by running BLAST on general or specialized databases. A guide tree then helps to select neighbor sequences to be used as input for the phylogeny pipeline. Phylogeny.fr is available at: http://www.phylogeny.fr
South Green Galaxy: a suite of tools for plant genomics
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Academic Year: 2000-2001https://scholarworks.sjsu.edu/production_images/2682/thumbnail.jp
Detection of gene orthology from gene co-expression and protein interaction networks
Background Ortholog detection methods present a powerful approach for finding genes that participate in similar biological processes across different organisms, extending our understanding of interactions between genes across different pathways, and understanding the evolution of gene families.
Results We exploit features derived from the alignment of protein-protein interaction networks and gene-coexpression networks to reconstruct KEGG orthologs for Drosophila melanogaster, Saccharomyces cerevisiae, Mus musculus and Homo sapiens protein-protein interaction networks extracted from the DIP repository and Mus musculus and Homo sapiens and Sus scrofa gene coexpression networks extracted from NCBI\u27s Gene Expression Omnibus using the decision tree, Naive-Bayes and Support Vector Machine classification algorithms.
Conclusions The performance of our classifiers in reconstructing KEGG orthologs is compared against a basic reciprocal BLAST hit approach. We provide implementations of the resulting algorithms as part of BiNA, an open source biomolecular network alignment toolkit