758 research outputs found
Coordinated induction of cell survival signaling in the inflamed microenvironment of the prostate
PURPOSE:
Both prostate cancer and benign prostatic hyperplasia are associated with inflammatory microenvironments. Inflammation is damaging to tissues, but it is unclear how the inflammatory microenvironment protects specialized epithelial cells that function to proliferate and repair the tissue. The objective of this study is to characterize the cell death and cell survival response of the prostatic epithelium in response to inflammation.
METHODS:
We assessed induction of cell death (TNF, TRAIL, TWEAK, FasL) and cell survival factors (IGFs, hedgehogs, IL-6, FGFs, and TGFs) in inflamed and control mouse prostates by ELISA. Cell death mechanisms were determined by immunoblotting and immunofluorescence for cleavage of caspases and TUNEL. Survival pathway activation was assessed by immunoblotting and immunofluorescence for Mcl-1, Bcl-2, Bcl-XL, and survivin. Autophagy was determined by immunoblotting and immunofluorescence for free and membrane associated light chain 3 (LC-3).
RESULTS:
Cleavage of all four caspases was significantly increased during the first 2 days of inflammation, and survival protein expression was substantially increased subsequently, maximizing at 3 days. By 5 days of inflammation, 50% of prostatic epithelial cells expressed survivin. Autophagy was also evident during the recovery phase (3 days). Finally, immunofluorescent staining of human specimens indicates strong activation of survival proteins juxtaposed to inflammation in inflamed prostate specimens.
CONCLUSIONS:
The prostate responds to deleterious inflammation with induction of cell survival mechanisms, most notably survivin and autophagy, demonstrating a coordinated induction of survival factors that protects and expands a specialized set of prostatic epithelial cells as part of the repair and recovery process during inflammation
Behavioural and pathomorphological impacts of flash photography on benthic fishes
Millions of people take animal pictures during wildlife interactions, yet the impacts of photographer behaviour and photographic flashes on animals are poorly understood. We investigated the pathomorphological and behavioural impacts of photographer behaviour and photographic flashes on 14 benthic fish species that are important for scuba diving tourism and aquarium displays. We ran a field study to test effects of photography on fish behaviour, and two laboratory studies that tested effects of photographic flashes on seahorse behaviour, and ocular and retinal anatomy. Our study showed that effects of photographic flashes are negligible and do not have stronger impacts than those caused solely by human presence. Photographic flashes did not cause changes in gross ocular and retinal anatomy of seahorses and did not alter feeding success. Physical manipulation of animals by photographing scuba divers, however, elicited strong stress responses. This study provides important new information to help develop efficient management strategies that reduce environmental impacts of wildlife tourism
Magnetic Coordinate Systems
Geospace phenomena such as the aurora, plasma motion, ionospheric currents
and associated magnetic field disturbances are highly organized by Earth's main
magnetic field. This is due to the fact that the charged particles that
comprise space plasma can move almost freely along magnetic field lines, but
not across them. For this reason it is sensible to present such phenomena
relative to Earth's magnetic field. A large variety of magnetic coordinate
systems exist, designed for different purposes and regions, ranging from the
magnetopause to the ionosphere. In this paper we review the most common
magnetic coordinate systems and describe how they are defined, where they are
used, and how to convert between them. The definitions are presented based on
the spherical harmonic expansion coefficients of the International Geomagnetic
Reference Field (IGRF) and, in some of the coordinate systems, the position of
the Sun which we show how to calculate from the time and date. The most
detailed coordinate systems take the full IGRF into account and define magnetic
latitude and longitude such that they are constant along field lines. These
coordinate systems, which are useful at ionospheric altitudes, are
non-orthogonal. We show how to handle vectors and vector calculus in such
coordinates, and discuss how systematic errors may appear if this is not done
correctly
Precision Measurement of the Mass Difference
We have measured the vector-pseudoscalar mass splitting , significantly more precise than the previous
world average. We minimize the systematic errors by also measuring the
vector-pseudoscalar mass difference using the radiative
decay , obtaining
. This is
then combined with our previous high-precision measurement of
, which used the decay . We also
measure the mass difference MeV, using the
decay modes of the and mesons.Comment: 18 pages uuencoded compressed postscript (process with uudecode then
gunzip). hardcopies with figures can be obtained by sending mail to:
[email protected]
Semileptonic Branching Fraction of Charged and Neutral B Mesons
An examination of leptons in events tagged by reconstructed
decays yields semileptonic branching fractions of for charged and for neutral mesons.
This is the first measurement for charged . Assuming equality of the charged
and neutral semileptonic widths, the ratio is
equivalent to the ratio of lifetimes. A postscript version is available through
World-Wide-Web in http://w4.lns.cornell.edu/public/CLNS/1994Comment: 9 pages (in REVTEX format) Preprint CLNS94-1286, CLEO 94-1
Observation of a New Charmed Strange Meson
Using the CLEO-II detector, we have obtained evidence for a new meson
decaying to . Its mass is
{}~MeV/ and its width is ~MeV/. Although we do not
establish its spin and parity, the new meson is consistent with predictions for
an , , charmed strange state.Comment: 9 pages uuencoded compressed postscript (process with uudecode then
gunzip). hardcopies with figures can be obtained by sending mail to:
[email protected]
On the origin of aurorae on Mars
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/95268/1/grl20829.pd
Sleep-wake sensitive mechanisms of adenosine release in the basal forebrain of rodents : an in vitro study
Adenosine acting in the basal forebrain is a key mediator of sleep homeostasis. Extracellular adenosine concentrations increase during wakefulness, especially during prolonged wakefulness and lead to increased sleep pressure and subsequent rebound sleep. The release of endogenous adenosine during the sleep-wake cycle has mainly been studied in vivo with microdialysis techniques. The biochemical changes that accompany sleep-wake status may be preserved in vitro. We have therefore used adenosine-sensitive biosensors in slices of the basal forebrain (BFB) to study both depolarization-evoked adenosine release and the steady state adenosine tone in rats, mice and hamsters. Adenosine release was evoked by high K+, AMPA, NMDA and mGlu receptor agonists, but not by other transmitters associated with wakefulness such as orexin, histamine or neurotensin. Evoked and basal adenosine release in the BFB in vitro exhibited three key features: the magnitude of each varied systematically with the diurnal time at which the animal was sacrificed; sleep deprivation prior to sacrifice greatly increased both evoked adenosine release and the basal tone; and the enhancement of evoked adenosine release and basal tone resulting from sleep deprivation was reversed by the inducible nitric oxide synthase (iNOS) inhibitor, 1400 W. These data indicate that characteristics of adenosine release recorded in the BFB in vitro reflect those that have been linked in vivo to the homeostatic control of sleep. Our results provide methodologically independent support for a key role for induction of iNOS as a trigger for enhanced adenosine release following sleep deprivation and suggest that this induction may constitute a biochemical memory of this state
The Electron Drift Instrument on Cluster: overview of first results
International audienceEDI measures the drift velocity of artificially injected electron beams. From this drift velocity, the perpendicular electric field and the local magnetic field gradients can be deduced when employing different electron energies. The technique requires the injection of two electron beams at right angles to the magnetic field and the search for those directions within the plane that return the beams to their associated detectors after one or more gyrations. The drift velocity is then derived from the directions of the two beams and/or from the difference in their times-of-flight, measured via amplitude-modulation and coding of the emitted electron beams and correlation with the signal from the returning electrons. After careful adjustment of the control parameters, the beam recognition algorithms, and the onboard magnetometer calibrations during the commissioning phase, EDI is providing excellent data over a wide range of conditions. In this paper, we present first results in a variety of regions ranging from the polar cap, across the magnetopause, and well into the magnetosheath
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