The Old Rustic Bridge By The Mill : Song And Chorus

https://digitalcommons.library.umaine.edu/mmb-vp/4022/thumbnail.jp

I\u27ll Wait For You Under The Willow : Song And Chorus

https://digitalcommons.library.umaine.edu/mmb-vp/1768/thumbnail.jp

The Fisher Girls Dream

https://digitalcommons.library.umaine.edu/mmb-vp/3952/thumbnail.jp

Strolling On The Brooklyn Bridge

https://digitalcommons.library.umaine.edu/mmb-vp/4057/thumbnail.jp

Patterns of gene expression in schistosomes: localization by whole mount in situ hybridization

rom the identification of genes to the characterization of their functions and interactions. Developmental biologists have long used whole mount in situ hybridization (WISH) to determine gene expression patterns, as a vital tool for formulating and testing hypotheses about function. This paper describes the application of WISH to the study of gene expression in larval and adult schistosomes. Fixed worms were permeablized by proteinase K treatment for hybridization with digoxygenin-labelled RNA probes, with binding being detected by alkaline phosphatase-coupled anti-digoxygenin antibodies, and BM Purple substrate. Discrete staining patterns for the transcripts of the molecules Sm29, cathepsin L, antigen 10.3 and chorion were observed in the tegument cell bodies, gut epithelium, oesophageal gland and vitelline lobules, respectively, of adult worms. Transcripts of the molecules SGTP4, GP18-22 and cathepsin L were localized to tegument cell bodies and embryonic gut, respectively, of lung schistosomula. We also showed that Fast Red TR fluorescent substrate can refine the pattern of localization permitting use of confocal microscopy. We believe that method of WISH will find broad application, in synergy with other emerging post-genomic techniques, such as RNA interference, to studies focused at increasing our molecular understanding of schistosomes

Computer recommendations for an automatic approach and landing system for V/STOL aircraft. Volume 2 - Equations

Automatic approach and landing system for V/STOL aircraf

Amino acid transport in schistosomes: Characterization of the permeaseheavy chain SPRM1hc

Schistosomes are human parasitic flatworms that constitute an important public health problem globally. Adult parasites live in the bloodstream where they import nutrients such as amino acids across their body surface (the tegument). One amino acid transporter, Schistosome Permease 1 light chain, SPRM1lc, a member of the glycoprotein-associated family of transporters (gpaAT), has been characterized in schistosomes. Only a single member of the SLC3 family of glycoproteins that associate with gpaATs is found following extensive searching of the genomes of Schistosoma mansoni and S. japonicum. In this report, we characterize this schistosome permease heavy chain (SPRM1hc) gene and protein. The 72-kDa gene product is predicted to possess a single transmembrane domain, a (betaalpha)(8) (TIM barrel) conformation and a catalytic triad. Xenopus oocytes functionally expressing SPRM1hc with SPRM1lc import phenylalanine, arginine, lysine, alanine, glutamine, histidine, tryptophan, and leucine. Biochemical characterization demonstrates that in Xenopus extracts and in schistosome extracts SPRM1hc is associated into a high molecular weight complex with SPRM1lc that is disrupted by reducing agents. Quantitative real-time PCR and Western analysis demonstrate that SPRM1hc is expressed in each schistosome life stage examined (eggs, cercariae, schistosomula, adult males and females). SPRM1hc is widely distributed throughout adult male and female worms as determined by immunolocalization. Consistent with the hypothesis that SPRM1hc functions to facilitate nutrient uptake from host blood, immunogold electron microscopy confirms that the protein is distributed on the host-interactive tegumental membranes. We propose that surface-exposed, host-interactive, nutrient-transporting proteins like the SPRM1 heterodimer are promising vaccine candidates

Cross-Disciplinary Genomics Approaches to Studying Emerging Fungal Infections

Emerging fungal pathogens pose a serious, global and growing threat to food supply systems, wild ecosystems, and human health. However, historic chronic underinvestment in their research has resulted in a limited understanding of their epidemiology relative to bacterial and viral pathogens. Therefore, the untargeted nature of genomics and, more widely, -omics approaches is particularly attractive in addressing the threats posed by and illuminating the biology of these pathogens. Typically, research into plant, human and wildlife mycoses have been largely separated, with limited dialogue between disciplines. However, many serious mycoses facing the world today have common traits irrespective of host species, such as plastic genomes; wide host ranges; large population sizes and an ability to persist outside the host. These commonalities mean that -omics approaches that have been productively applied in one sphere and may also provide important insights in others, where these approaches may have historically been underutilised. In this review, we consider the advances made with genomics approaches in the fields of plant pathology, human medicine and wildlife health and the progress made in linking genomes to other -omics datatypes and sets; we identify the current barriers to linking -omics approaches and how these are being underutilised in each field; and we consider how and which -omics methodologies it is most crucial to build capacity for in the near future

The other side of “getting by”: A case study of interpreting provision decision-making and consequences for patients

Rates of provision of professional interpreting services to patients have been shown to be low in hospital emergency departments and wards. This study aimed to elicit the reasons for the context and consequences of non-provision of professional interpreting at an adult hospital and identify strategies to increase provision. This was a qualitative case study of multiple perspectives (including patients, family members, interpreters, clinical and administrative hospital staff) using in-depth interviews, participant-observation, focus group discussions, staff survey and medical record review. The data were analysed using thematic, content and systems thinking analysis to develop a theoretical framework for providers’ decision-making processes and contextual constraints. The patient and family perspectives showed that ad hoc communication negatively affected their hospital experience and patient-centred care, and highlighted errors in communication largely unknown to treating staff. Key reasons shown for low rates of professional interpreter engagement by staff were: (1) a lack of familiarity and clarity of the process of engaging interpreters combined with inadequate infrastructure, (2) low levels of trust in and confidence in working with professional interpreters and (3) little knowledge of the evidence-base (including cost-benefit) or negative consequences resulting from “getting by”. The study shows that influencing norms through (a) clarifying the pathway from identification of need to engagement of interpreters, (b) provision of clinical staff training on the evidence base and role of interpreter (c) influencing training and professionalism of interpreting are important for improving professional interpreting provision rates in a hospital setting