Measurements of muon flux in the Pyh\"asalmi underground laboratory

The cosmic-ray induced muon flux was measured at several depths in the Pyh\"asalmi mine (Finland) using a plastic scintillator telescope mounted on a trailer. The flux was determined at four different depths underground at 400 m (980 m.w.e), at 660 m (1900 m.w.e), at 990 m (2810 m.w.e) and at 1390 m (3960 m.w.e) with the trailer, and also at the ground surface. In addition, previously measured fluxes from depths of 90 m (210 m.w.e) and 210 m (420 m.w.e) are shown. A relation was obtained for the underground muon flux as a function of the depth. The measured flux follows well the general behaviour and is consistent with results determined in other underground laboratories.Comment: 8 pages, 2 figures. Submitted to Nuclear Instrum. Methods

Evidence for Strain-Induced Ferroelectric Order in Epitaxial Thin-Film KTaO3

In perovskite-structure epitaxial films, it has been theoretically predicted that the polarization and the coherence of polar order can increase with increasing crystallographic strain. Experimental evidence of strain-induced long-range ferroelectric order has not been obtained thus far, posing the fundamental question of whether or not strain can induce the long-range polar order. Here we demonstrate the existence of strain-induced ferroelectric order in quantum paraelectric KTaO3 by combining experimental investigations of epitaxial KTaO3 films and density-functional-theory calculations. The long-range ferroelectric order does exist under a large enough epitaxial strain. We suggest that a region of short-range polar order might appear between paraelectric and ferroelectric states in the strain-temperature phase diagrams.Peer reviewe

Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate : comparison to rat cortical cultures

Human pluripotent stem cell (hPSC)-derived neurons provide exciting opportunities for in vitro modeling of neurological diseases and for advancing drug development and neurotoxicological studies. However, generating electrophysiologically mature neuronal networks from hPSCs has been challenging. Here, we report the differentiation of functionally active hPSC-derived cortical networks on defined laminin-521 substrate. We apply microelectrode array (MEA) measurements to assess network events and compare the activity development of hPSC-derived networks to that of widely used rat embryonic cortical cultures. In both of these networks, activity developed through a similar sequence of stages and time frames; however, the hPSC-derived networks showed unique patterns of bursting activity. The hPSC-derived networks developed synchronous activity, which involved glutamatergic and GABAergic inputs, recapitulating the classical cortical activity also observed in rodent counterparts. Principal component analysis (PCA) based on spike rates, network synchronization and burst features revealed the segregation of hPSC-derived and rat network recordings into different clusters, reflecting the species-specific and maturation state differences between the two networks. Overall, hPSC-derived neural cultures produced with a defined protocol generate cortical type network activity, which validates their applicability as a human-specific model for pharmacological studies and modeling network dysfunctions.Peer reviewe

EMMA - A New Underground Cosmic-Ray Experiment

A new type of cosmic-ray experiment is under construction in the Pyh\"asalmi mine in the underground laboratory of the University of Oulu, Finland. It aims to study the composition of cosmic rays at and above the knee region. The experiment, called EMMA, will cover approximately 150 square-metres of detector area. The array is capable of measuring the multiplicity and the lateral distribution of underground muons, and the arrival direction of the air shower. The full-size detector is expected to run by the end of 2007.Comment: Extended and updated TAUP2005 Proceedings contribution. 8 pages, 5 figures (part in colour). Preprint not submitte

Efflux Protein Expression in Human Stem Cell-Derived Retinal Pigment Epithelial Cells

Retinal pigment epithelial (RPE) cells in the back of the eye nourish photoreceptor cells and form a selective barrier that influences drug transport from the blood to the photoreceptor cells. At the molecular level, ATP-dependent efflux transporters have a major role in drug delivery in human RPE. In this study, we assessed the relative expression of several ATP-dependent efflux transporter genes (MRP1, -2, -3, -4, -5, -6, p-gp, and BCRP), the protein expression and localization of MRP1, MRP4, and MRP5, and the functionality of MRP1 efflux pumps at different maturation stages of undifferentiated human embryonic stem cells (hESC) and RPE derived from the hESC (hESC-RPE). Our findings revealed that the gene expression of ATP-dependent efflux transporters MRP1, -3, -4, -5, and p-gp fluctuated during hESC-RPE maturation from undifferentiated hESC to fusiform, epithelioid, and finally to cobblestone hESC-RPE. Epithelioid hESC-RPE had the highest expression of MRP1, -3, -4, and P-gp, whereas the most mature cobblestone hESC-RPE had the highest expression of MRP5 and MRP6. These findings indicate that a similar efflux protein profile is shared between hESC-RPE and the human RPE cell line, ARPE-19, and suggest that hESC-RPE cells are suitable in vitro RPE models for drug transport studies. Embryonic stem cell model might provide a novel tool to study retinal cell differentiation, mechanisms of RPE -derived diseases, drug testing and targeted drug therapy

Seismic observations at the Sodankylä Geophysical Observatory: history, present, and the future

Instrumental seismic observations in northern Finland started in the 1950s. They were originally initiated by the Institute of Seismology of the University of Helsinki (ISUH), but the staff of Sodankylä Geophysical Observatory (SGO) and later geophysicists of the University of Oulu (UO) were involved in the development of seismological observations and research in northern Finland from the very beginning. This close cooperation between seismologists and the technical staff of ISUH, UO, and SGO continued in many significant international projects and enabled a high level of seismological research in Finland. In our paper, we present history and current status of seismic observations and seismological research in northern Finland at the UO and SGO. These include both seismic observations at permanent seismic stations and temporary seismic experiments with portable seismic equipment. We describe the present seismic instrumentation and major research topics of the seismic group at SGO and discuss plans for future development of permanent seismological observations and portable seismic instrumentation at SGO as part of the European Plate Observing System (EPOS) research infrastructure. We also present the research topics of the recently organized Laboratory of Applied Seismology, and show examples of seismic observations performed by new seismic equipment located at this laboratory and selected results of time-lapse seismic body wave travel-time tomography using the data of microseismic monitoring in the Pyhäsalmi Mine (northern Finland)

Improving the quality of empirical Green's functions, obtained by cross-correlation of high-frequency ambient seismic noise

Abstract Studying the uppermost structure of the subsurface is a necessary part of solving many practical problems (exploration of minerals, groundwater studies, geoengineering, etc.). The practical application of active seismic methods for these purposes is not always possible for different reasons, such as logistical difficulties, high cost of work, and a high level of seismic and acoustic noise. That is why developing and improving passive seismic methods is one of the important problems in applied geophysics. In our study, we describe a way of improving the quality of empirical Green’s functions (EGFs), evaluated from high-frequency ambient seismic noise, by using the advanced technique of cross-correlation function stacking in the time domain (in this paper we use term “high-frequency” for frequencies higher than 1 Hz). The technique is based on the global optimization algorithm, in which the optimized objective function is a signal-to-noise ratio of an EGF, retrieved at each iteration. In comparison to existing techniques, based, for example, on weight stacking of cross-correlation functions, our technique makes it possible to significantly increase the signal-to-noise ratio and, therefore, the quality of the EGFs. The technique has been tested with the field data acquired in an area with a high level of industrial noise (Pyhäsalmi Mine, Finland) and in an area with a low level of anthropogenic noise (Kuusamo Greenstone Belt, Finland). The results show that the proposed technique can be used for the extraction of EGFs from high-frequency seismic noise in practical problems of mapping of the shallow subsurface, both in areas with high and low levels of high-frequency seismic noise

Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures

Funder: Tampereen Yliopisto (University of Tampere); doi: https://doi.org/10.13039/501100004371Funder: Tekes (Finnish Funding Agency for Innovation); doi: https://doi.org/10.13039/501100003406Abstract: Human pluripotent stem cell (hPSC)-derived neurons provide exciting opportunities for in vitro modeling of neurological diseases and for advancing drug development and neurotoxicological studies. However, generating electrophysiologically mature neuronal networks from hPSCs has been challenging. Here, we report the differentiation of functionally active hPSC-derived cortical networks on defined laminin-521 substrate. We apply microelectrode array (MEA) measurements to assess network events and compare the activity development of hPSC-derived networks to that of widely used rat embryonic cortical cultures. In both of these networks, activity developed through a similar sequence of stages and time frames; however, the hPSC-derived networks showed unique patterns of bursting activity. The hPSC-derived networks developed synchronous activity, which involved glutamatergic and GABAergic inputs, recapitulating the classical cortical activity also observed in rodent counterparts. Principal component analysis (PCA) based on spike rates, network synchronization and burst features revealed the segregation of hPSC-derived and rat network recordings into different clusters, reflecting the species-specific and maturation state differences between the two networks. Overall, hPSC-derived neural cultures produced with a defined protocol generate cortical type network activity, which validates their applicability as a human-specific model for pharmacological studies and modeling network dysfunctions