Pain and mental health - separate and joint associations with sickness absence among young employees

Peer reviewe

Assessing the discharge instructing in the emergency department : Patient perspective

Objective: The objective of the study is to assess how well the emergency department (ED) personnel succeed in instructing the patient at discharge. Methods: In November and December 2016 at Peijas Hospital ED, Finland, a structured questionnaire was conducted during a phone interview on patients the day after discharge. Results: A total of 132 patients interviewed. Ninety percent had received discharge instructions from the ED staff, most of them (75%) about medication. Almost half of the patients (45%) were satisfied with the communication at discharge, those not satisfied (47%) felt that the staff did not know enough of their background to give discharge instructions. Of the patients, 20% thought that they did not have the opportunity to ask questions during the guidance session, and 41% thought that the session was too short and restricted. Some patients (20%) felt that the instructions were ambiguous, but 63% (83/132) felt they were able to follow them well or very well. Conclusion: The pace of care in the ED is fast and duration of the stay is short. The patients must be able to take responsibility of their self-care. Failure to follow medical discharge instructions could lead to non-compliance. Attention should be paid to enhancing the quality of discharge instructing and the instructions provided by the ED personnel, as recurring visits and inquiry calls add to the ED workload.Peer reviewe

The construction and validation of a short form of the developmental, diagnostic and dimensional interview

We aimed to construct and validate a shortened form of the developmental, diagnostic and dimensional interview (3Di), a parent report interview for assessing and diagnosing autistic spectrum disorders (ASDs). Data from 879 children and young people were used. In half of the sample (n = 440) reliability analysis was used to identify 3Di items that best measured each dimension of the autism triad. This informed the construction of a shortened (53 item) 3Di, which was then validated on subjects not used in the reliability analysis (n = 439). This involved comparison with scores from the original 3Di algorithm and, in a subsample (n = 29), with the autism diagnostic interview-revised (ADI-R). Agreement of the new shortened 3Di with the 3Di’s original algorithm was excellent in both dimensional and categorical terms. Agreement on caseness (27 out of 29) with the ADI-R was also strong. The new 3Di short version is less than half as long as the original version and outputs very similar scores. It will be useful to clinicians and researchers for obtaining a dimensional autism assessment in less than 45 minutes

Effect of High-Dose Esomeprazole on CYP1A2, CYP2C19, and CYP3A4 Activities in Humans : Evidence for Substantial and Long-lasting Inhibition of CYP2C19

In vitro, esomeprazole is a time-dependent inhibitor of CYP2C19. Additionally, racemic omeprazole induces CYP1A2 and omeprazole and its metabolites inhibit CYP3A4in vitro. In this 5-phase study, 10 healthy volunteers ingested 20 mg pantoprazole, 0.5 mg midazolam, and 50 mg caffeine as respective index substrates for CYP2C19, 3A4, and 1A2 before and 1, 25, 49 (pantoprazole only), and 73 hours after an 8-day pretreatment with 80 mg esomeprazole twice daily. The area under the plasma concentration-time curve (AUC) ofR-pantoprazole increased 4.92-fold (90% confidence interval (CI) 3.55-6.82), 2.31-fold (90% CI 1.85-2.88), and 1.33-fold (90% CI 1.06-1.68) at the 1-hour, 25-hour, and 73-hour phases, respectively, consistent with a substantial and persistent inhibition of CYP2C19. The AUC of midazolam increased up to 1.44-fold (90% CI 1.22-1.72) and the paraxanthine/caffeine metabolic ratio up to 1.19-fold (90% CI 1.04-1.36), when the index substrates were taken 1 hour after esomeprazole. Based on the recovery ofR-pantoprazole oral clearance, the turnover half-life of CYP2C19 was estimated to average 53 hours. Pharmacokinetic simulation based on the observed concentrations of esomeprazole and its metabolites as well as their published CYP2C19 inhibitory constants was well in line with the observed changes inR-pantoprazole pharmacokinetics during the course of the study. Extrapolations assuming linear pharmacokinetics of esomeprazole suggested weak to moderate inhibition at 20 and 40 mg twice daily dosing. In conclusion, high-dose esomeprazole can cause strong inhibition of CYP2C19, but only weakly inhibits CYP3A4 and leads to minor induction of CYP1A2. The enzymatic activity of CYP2C19 recovers gradually in similar to 3-4 days after discontinuation of esomeprazole treatment.Peer reviewe

TiF1-Gamma Plays an Essential Role in Murine Hematopoiesis and Regulates Transcriptional Elongation of Erythroid Genes

Transcriptional regulators play critical roles in the regulation of cell fate during hematopoiesis. Previous studies in zebrafish have identified an essential role for the transcriptional intermediary factor TIF1γ in erythropoiesis by regulating the transcription elongation of erythroid genes. To study if TIF1γ plays a similar role in murine erythropoiesis and to assess its function in other blood lineages, we generated mouse models with hematopoietic deletion of TIF1γ. Our results showed a block in erythroid maturation in the bone marrow following tif1γ deletion that was compensated with enhanced spleen erythropoiesis. Further analyses revealed a defect in transcription elongation of erythroid genes in the bone marrow. In addition, loss of TIF1γ resulted in defects in other blood compartments, including a profound loss of B cells, a dramatic expansion of granulocytes and decreased HSC function. TIF1γ exerts its functions in a cell-autonomous manner as revealed by competitive transplantation experiments. Our study therefore demonstrates that TIF1γ plays essential roles in multiple murine blood lineages and that its function in transcription elongation is evolutionally conserved.Stem Cell and Regenerative Biolog

Power line mapping technique using all-terrain mobile laser scanning

Power line mapping using remote sensing can automate the traditionally labor-intensive power line corridor inspection. Land-based mobile laser scanning (MLS) can be a good choice for the power line mapping if an aerial inspection is impossible, too costly or slow, unsafe, prohibited by regulations, or if more detailed information on the power line corridor is needed. The mapping of the power lines using MLS was studied in a rural environment outside the road network for the first time. An automatic power line extraction algorithm was developed. The algorithm first found power line candidate points based on the shape and orientation of the local neighborhood of a point using principal component analysis. Power lines were retrieved from the candidates using random sample consensus (Ransac) and a new power line labeling method, which takes into account the three-dimensional shape of the power lines. The new labeling method was able to find the power lines and remove false detections, which were found, for example, from the forest. The algorithm was tested in forested and open field (arable land) areas, outside the road environment using two different platforms of MLS, namely, personal backpack and all-terrain vehicle. The recall and precision of the power line extraction were 93.3% and 93.6%, respectively, using 10 cm as a distance criterion for a successful detection. Drifting of the positioning solution of the scanner was the largest error source, being the (contributory) cause for 60–70% of the errors. The platform did not have a significant effect on the power line extraction accuracy. The accuracy was higher in the open field compared to the forest, because the one-dimensional point density along the power line was inhomogeneous and GNSS (global navigation satellite system) signal was weak in the forest. The results suggest that the power lines can be mapped accurately enough for inspection purposes using MLS in a rural environment outside the road network.</p

Preregistration Classification of Mobile LIDAR Data Using Spatial Correlations

We explore a novel paradigm for light detection and ranging (LIDAR) point classification in mobile laser scanning (MLS). In contrast to the traditional scheme of performing classification for a 3-D point cloud after registration, our algorithm operates on the raw data stream classifying the points on-the-fly before registration. Hence, we call it preregistration classification (PRC). Specifically, this technique is based on spatial correlations, i.e., local range measurements supporting each other. The proposed method is general since exact scanner pose information is not required, nor is any radiometric calibration needed. Also, we show that the method can be applied in different environments by adjusting two control parameters, without the results being overly sensitive to this adjustment. As results, we present classification of points from an urban environment where noise, ground, buildings, and vegetation are distinguished from each other, and points from the forest where tree stems and ground are classified from the other points. As computations are efficient and done with a minimal cache, the proposed methods enable new on-chip deployable algorithmic solutions. Broader benefits from the spatial correlations and the computational efficiency of the PRC scheme are likely to be gained in several online and offline applications. These range from single robotic platform operations including simultaneous localization and mapping (SLAM) algorithms to wall-clock time savings in geoinformation industry. Finally, PRC is especially attractive for continuous-beam and solid-state LIDARs that are prone to output noisy data

Morphological changes and riffle-pool dynamics related to flow in a meandering river channel based on a 5-year monitoring period using close-range remote sensing

The maintenance of riffle-pool sequences and morphological changes in the long-term have received little attention in the literature. The aims of this study are to determine morphological changes and riffle-pool maintenance in relation to flow conditions in a meandering river channel over a 5-yr period. Change detection was focused on riffle and pool maintenance in a river reach covering three successive meander bends. Changes in a meandering river channel were studied utilizing detailed digital terrain models and flow data. The results indicated that riffle-pool sequences are maintained by high discharge events and the development of pools and riffles was linked. During high discharges, the riverbed eroded on the concave sides and the inflexion points aggraded, causing riffle–pool sequences, whereas during low discharges, concave sides aggraded and inflexion points eroded, causing pool filling and riffle erosion. While discharge increased, near-bed flow velocities increased faster on the concave sides of the bends than at the inflexion points, becoming higher at a discharge of 8 m3/s, ~20% of the bankfull discharge. Changes in the three successive meander bends were mainly similar, and the geometry of meandering rivers contributed to the locations of riffles and pools. Pools and riffles were not stable in size and shape, but their longitudinal location remained the same, instead of migrating up and down the channel. Morphological changes occurred in meander bends year-round, but they were non-linear. Annual channel change was not similar from year to year owing to different flow regimes and morphological changes during the previous year. However, seasonal detection revealed similarities between high and low discharge periods between the years. Concave sides of meander bends may act to temporarily store sediment; however, storage is preserved only under the effective hydrological discharge.</p

Integration of IRF6 and Jagged2 signalling is essential for controlling palatal adhesion and fusion competence

In mammals, adhesion and fusion of the palatal shelves are essential mechanisms during the development of the secondary palate; failure of these processes leads to the congenital anomaly, cleft palate. The mechanisms that prevent pathological adhesion between the oral and palatal epithelia while permitting adhesion and subsequent fusion of the palatal shelves via their medial edge epithelia remain obscure. In humans, mutations in the transcription factor interferon regulatory factor 6 (IRF6) underlie Van der Woude syndrome and popliteal pterygium syndrome. Recently, we have demonstrated that mice homozygous for a mutation in Irf6 exhibit abnormalities of epithelial differentiation that results in cleft palate as a consequence of adhesion between the palatal shelves and the tongue. In the current paper, we demonstrate that Irf6 is essential for oral epithelial differentiation and that IRF6 and the Notch ligand Jagged2 function in convergent molecular pathways during this process. We further demonstrate that IRF6 plays a key role in the formation and maintenance of the oral periderm, spatio-temporal regulation of which is essential for ensuring appropriate palatal adhesion