Human immunodeficiency virus infection of the human thymus and disruption of the thymic microenvironment in the SCID-hu mouse.

Infection with the human immunodeficiency virus (HIV) results in immunosuppression and depletion of circulating CD4+ T cells. Since the thymus is the primary organ in which T cells mature it is of interest to examine the effects of HIV infection in this tissue. HIV infection has been demonstrated in the thymuses of infected individuals and thymocytes have been previously demonstrated to be susceptible to HIV infection both in vivo, using the SCID-hu mouse, and in vitro. The present study sought to determine which subsets of thymocytes were infected in the SCID-hu mouse model and to evaluate HIV-related alterations in the thymic microenvironment. Using two different primary HIV isolates, infection was found in CD4+/CD8+ double positive thymocytes as well as in both the CD4+ and CD8+ single positive subsets of thymocytes. The kinetics of infection and resulting viral burden differed among the three thymocyte subsets and depended on which HIV isolate was used for infection. Thymic epithelial (TE) cells were also shown to endocytose virus and to often contain copious amounts of viral RNA in the cytoplasm by in situ hybridization, although productive infection of these cells could not be definitively shown. Furthermore, degenerating TE cells were observed even without detection of HIV in the degenerating cells. Two striking morphologic patterns of infection were seen, involving either predominantly thymocyte infection and depletion, or TE cell involvement with detectable cytoplasmic viral RNA and/or TE cell toxicity. Thus, a variety of cells in the human thymus is susceptible to HIV infection, and infection with HIV results in a marked disruption of the thymic microenvironment leading to depletion of thymocytes and degeneration of TE cells

The first Frontier Fields cluster: 4.5{\mu}m excess in a z~8 galaxy candidate in Abell 2744

We present in this letter the first analysis of a z~8 galaxy candidate found in the Hubble and Spitzer imaging data of Abell 2744, as part of the Hubble Frontier Fields legacy program. We applied the most commonly-used methods to select exceptionally high-z galaxies by combining non-detection and color-criteria using seven HST bands. We used GALFIT on IRAC images for fitting and subtracting contamination of bright nearby sources. The physical properties have been inferred from SED-fitting using templates with and without nebular emission. This letter is focussed on the brightest candidate we found (m$_{F160W}$=26.2) over the 4.9 arcmin$^2$ field of view covered by the WFC3. It shows a non-detection in the ACS bands and at 3.6{\mu}m whereas it is clearly detected at 4.5{\mu}m with rather similar depths. This break in the IRAC data could be explained by strong [OIII]+H{\beta} lines at z~8 which contribute to the 4.5{\mu}m photometry. The best photo-z is found at z~8.0$^{+0.2}_{-0.5}$, although solutions at low-redshift (z~1.9) cannot be completely excluded, but they are strongly disfavoured by the SED-fitting work. The amplification factor is relatively small at {\mu}=1.49$\pm$0.02. The Star Formation Rate in this object is ranging from 8 to 60 Mo/yr, the stellar mass is in the order of M$_{\star}$=(2.5-10) x 10$^{9}$Mo and the size is r~0.35$\pm$0.15 kpc. This object is one of the first z~8 LBG candidates showing a clear break between 3.6{\mu}m and 4.5{\mu}m which is consistent with the IRAC properties of the first spectroscopically confirmed galaxy at a similar redshift. Due to its brightness, the redshift of this object could potentially be confirmed by near infrared spectroscopy with current 8-10m telescopes. The nature of this candidate will be revealed in the coming months with the arrival of new ACS and Spitzer data, increasing the depth at optical and near-IR wavelengths.Comment: 4 pages, 2 figures, Accepted for publication in Astronomy and Astrophysics Letter

The roles of convection, extratropical mixing, and in-situ freeze-drying in the Tropical Tropopause Layer

Mechanisms for transporting and dehydrating air across the tropical tropopause layer (TTL) are investigated with a conceptual two dimensional (2-D) model. The 2-D TTL model combines the Holton and Gettelman cold trap dehydration mechanism (Holton and Gettelman, 2001) with the two column convection model of Folkins and Martin (2005). We investigate 3 possible transport scenarios through the TTL: 1) slow uniform ascent across the level of zero radiative heating without direct convective mixing, 2) convective mixing of H<sub>2</sub>O vapor at 100% relative humidity with respect to ice (RHi) with no ice retention, and 3) convective mixing of extremely subsaturated air (100% RHi following the moist adiabatic temperature above the level of neutral buoyancy) with sufficient ice retention such that total H<sub>2</sub>O is 100%RHi. The three mechanisms produce similar seasonal cycles for H<sub>2</sub>O that are in good quantitative agreement with the Aura Microwave Limb Sounder (MLS) measurements. We use Aura MLS measurement of CO and Atmospheric Chemistry Experiment-Fourier Transform Spectrometer measurement of HDO to distinguish among the transport mechanisms. Model comparisons with the observations support the view that H<sub>2</sub>O is predominantly controlled by regions having the lowest cold point tropopause temperature but the trace species CO and HDO support the convective mixing of dry air and lofted ice. The model provides some insight into the processes affecting the long term trends observed in stratospheric H<sub>2</sub>O

Reductive Cleavage of Organic Peroxides by Iron Salts and Thiols

Despite the low bond strength of the oxygen− oxygen bond, organic peroxides are often surprisingly resistant to cleavage by nucleophiles and reductants. As a result, achieving decomposition under mild conditions can be challenging. Herein, we explore the reactivity of a selection of peroxides toward thiolates, phenyl selenide, Fe(II) salts, and iron thiolates. Peroxides activated by conjugation, strain, or stereoelectronics are rapidly cleaved at room temperature by thiolate anions, phenylselenide, or Fe(II) salts. Under the same conditions, unhindered dialkyl peroxides are only marginally reactive; hindered peroxides, including triacetone triperoxide and diacetone diperoxide (DADP), are inert. In contrast, all but the most hindered of peroxides are rapidly (\u3c1 min at concentrations down to ∼40 mM) cleaved by mixtures of thiols and iron salts. Our observations suggest the possible intermediacy of strongly reducing complexes that are readily regenerated in the presence of stoichiometric thiolate or hydride. In the case of DADP, an easily prepared explosive of significant societal concern, catalytic amounts of iron and thiol are capable of promoting rapid and complete disproportionation. The availability of inexpensive and readily available catalysts for the mild reductive degradation of all but the most hindered of peroxides could have significant applications for controlled remediation of explosives or unwanted radical initiators

Sampling Local Fungal Diversity in an Undergraduate Laboratory using DNA Barcoding

Traditional methods for fungal species identification require diagnostic morphological characters and are often limited by the availability of fresh fruiting bodies and local identification resources. DNA barcoding offers an additional method of species identification and is rapidly developing as a critical tool in fungal taxonomy. As an exercise in an undergraduate biology course, we identified 9 specimens collected from the Hendrix College campus in Conway, Arkansas, USA to the genus or species level using morphology. We report that DNA barcoding targeting the internal transcribed spacer (ITS) region supported several of our taxonomic determinations and we were able to contribute 5 ITS sequences to GenBank that were supported by vouchered collection information. We suggest that small-scale barcoding projects are possible and that they have value for documenting fungal diversity

Why, what, and how? case study on law, risk, and decision making as necessary themes in built environment teaching

The paper considers (and defends) the necessity of including legal studies as a core part of built environment undergraduate and postgraduate curricula. The writer reflects upon his own experience as a lawyer working alongside and advising built environment professionals in complex land remediation and site safety management situations in the United Kingdom and explains how themes of liability, risk, and decision making can be integrated into a practical simulation in order to underpin more traditional lecture-based law teaching. Through reflection upon the writer's experiments with simulation-based teaching, the paper suggests some innovations that may better orientate law teaching to engage these themes and, thereby, enhance the relevance of law studies to the future needs of built environment professionals in practice.</p