976 research outputs found

    Inactivation of metalloenzymes by salicylate

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    Applicability of molding procedures in laboratory mix tests for quality control and assurance of the deep mixing method

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    The deep mixing method (DMM) has been applied in many construction projects. The laboratory mix test is essential to the quality control and quality assurance (QC/QA) of deep mixing methods. The procedures used for the preparation of specimens in the laboratory mix test greatly affect the physical and mechanical properties of the stabilized soils. Different procedures are applied in different countries/regions. With the increasingly globalized DMM market, it is desirable that a common understanding of the nature of the laboratory mix test and internationally accepted guidelines to conduct it be established in order to guarantee the QC/QA of DMMs. As part of an international collaborative study, the influence of different molding techniques for the laboratory preparation of specimens was studied. Five different molding techniques were tested in four organizations. The results showed that the molding techniques considerably influenced the magnitude and variation of the unconfined compressive strength and the wet unit weight of the stabilized specimens. The applicability of the molding techniques was discussed in terms of their undrained shear strength and the liquidity index of the soil and binder mixture, and the usefulness of the techniques was demonstrated. (C) 2015 The Japanese Geotechnical Societ

    Finite element analysis of hydrogen retention in ITER plasma facing components using FESTIM

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    The behaviour of hydrogen isotopes in ITER monoblocks was studied using the code FESTIM (Finite Element Simulation of Tritium In Materials) which is introduced in this publication. FESTIM has been validated by reproducing experimental data and the Method of Manufactured Solutions was used for analytical verification. Following relevant plasma scenarios, both transient heat transfer and hydrogen isotopes (HIs) diffusion have been simulated in order to assess HIs retention in monoblocks. Relevant materials properties have been used. Each plasma cycle is composed of a current ramp up, a current plateau, a current ramp down and a resting phase before the following shot. 100 cycles are simulated. The total HIs inventory in the tokamak during resting phases reaches 1.8 x 10(-3) mgwhereas during the implantation phases it keeps increasing as a power law of time. Particle flux on the cooling channel of the monoblock is also computed. The breakthrough time is estimated to be t = 1 x 10(5) s which corresponds to 24 cycles. Relevance of 2D modelling has been demonstrated by comparing the total HIs inventory obtained by 2D and 1D simulations. Using 1D simulations, a relative error is observed compared to 2D simulations which can reach -25% during the resting phase. The error during implantation phases keeps increasing.Peer reviewe

    Myostatin (GDF8) single nucleotide polymorphisms in Nellore cattle.

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    The myostatin gene, also known as GDF8 (growth differentiation factor 8), is located on bovine chromosome 2 (BTA2); it has three exons and two introns. Myostatin is specifically expressed during embryonic development and in adult skeletal muscle, functioning as a negative regulatory protein. Several cattle breeds (Piedmontese, Belgian Blue and Blond?Aquitaine, and others) show polymorphisms in this gene; these polymorphisms are directly related to the double muscling phenotype. We looked for polymorphisms in the Nellore cattle myostatin gene and compared them with those known for taurine breeds. Seven regions, covering the three exons of this gene, were amplified by polymerase chain reaction and sequenced, including the untranslated region. DNA from 30 adult Nellore animals was collected; DNA sequencing revealed three, seven and four polymorphisms in exons 1, 2 and 3, respectively. We found previously reported polymorphisms, as well as several new ones; for instance, 37 polymorphisms were found in the untranslated region segment, and in introns 1 and 2 there were one and three polymorphisms, respectively. The high degree of allelic heterogeneity in the myostatin gene could be related to its high mutation rate; it also could be the result of a long history of artificial selection for meat production, which has probably favored such modifications and maintained them in cattle populations. These polymorphisms identified in Nellore cattle could be useful for breeding programs

    Toxicity and genotoxicity of wastewater from gasoline stations

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    The toxicity and genotoxicity of wastewater from eight gasoline stations in BrasĂ­lia, Brazil's capital city, was studied by assessing chromosomal aberrations, chromosomal malsegregation and the mitotic index in Alliumcepa root cells, and the occurrence of micronucleus and nuclear abnormalities in peripheral erythrocytes of tilapia fish (Oreochromis niloticus). The content of gasoline station effluents was also analyzed based on several physico-chemical parameters. None of the wastewater samples was genotoxic to A. cepa root cells, although cell proliferation was significantly inhibited, especially at the highest concentrations. Likewise, no micronuclei were observed in O. niloticus peripheral erythrocytes, even after exposure to high concentrations, but there was an increase in the number of nuclear abnormalities and fish mortality. These results show that although the effluent from gasoline stations is processed by an oil/water separation system before being discharged into the main sewage system, the wastewater still contains toxic compounds

    Dynamic fuel retention in tokamak wall materials: An in situ laboratory study of deuterium release from polycrystalline tungsten at room temperature

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    International audienceRetention of deuterium ion implanted in polycrystalline tungsten samples is studied in situ in an ultra-high vacuum apparatus equipped with a low-flux ion source and a high sensitivity thermo-desorption setup. Retention as a function of ion fluence was measured in the 10^17 -10^21 D+/m^2 range. By combining this new fluence range with the literature in situ experimental data, we evidence the existence of a retention = fluence^ 0.645±0.025 relationship which describes deuterium retention behavior on polycrystalline tungsten on 8 orders of magnitude of fluence. Evolution of deuterium retention as a function of the sample storage time in vacuum at room temperature was followed. A loss of 50% of the retained deuterium is observed when the storage time is increased from 2 h to 135 h. The role of the surface and of natural bulk defects on the deuterium retention/release in polycrystalline tungsten is discussed in light of the behavior of the single desorption peak obtained with Temperature Programmed Desorption
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