45 research outputs found
Mouse hitchhiker mutants have spina bifida, dorso-ventral patterning defects and polydactyly: identification of Tulp3 as a novel negative regulator of the Sonic hedgehog pathway
The mammalian Sonic hedgehog (Shh) signalling pathway is essential for embryonic development and the patterning of multiple organs. Disruption or activation of Shh signalling leads to multiple birth defects, including holoprosencephaly, neural tube defects and polydactyly, and in adults results in tumours of the skin or central nervous system. Genetic approaches with model organisms continue to identify novel components of the pathway, including key molecules that function as positive or negative regulators of Shh signalling. Data presented here define Tulp3 as a novel negative regulator of the Shh pathway. We have identified a new mouse mutant that is a strongly hypomorphic allele of Tulp3 and which exhibits expansion of ventral markers in the caudal spinal cord, as well as neural tube defects and preaxial polydactyly, consistent with increased Shh signalling. We demonstrate that Tulp3 acts genetically downstream of Shh and Smoothened (Smo) in neural tube patterning and exhibits a genetic interaction with Gli3 in limb development. We show that Tulp3 does not appear to alter expression or processing of Gli3, and we demonstrate that transcriptional regulation of other negative regulators (Rab23, Fkbp8, Thm1, Sufu and PKA) is not affected. We discuss the possible mechanism of action of Tulp3 in Shh-mediated signalling in light of these new data
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DECwrite v2.0: A novice`s perspective
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Does starvation-resistance in flies without octopamine explain differences in sucrose preference and learning?
New Dimeric Trigonal Bipyramidal 5-ansa-Lanthanocenes. X-ray Crystal structure of {[2,6-(CH2C5H4)2C5H3N]Yb(III)OSO2CF3}2, and the Kinetics of the transoid-cisoid rearrangement of the analogous Yttrium complex
New Dimeric Trigonal Bipyramidal 5-ansa-Lanthanocenes. X-ray Crystal structure of {[2,6-(CH2C5H4)2C5H3N]Yb(III)OSO2CF3}2, and the Kinetics of the transoid-cisoid rearrangement of the analogous Yttrium complex
Neue dimere, trigonal bipyramidale 5-ansa-Lanthanocene: Einkristallröntgen -strukturen von [{2,6-(CH2C5H4)2C5H3N}Ln(III)OSO2CF3]2 mit Ln = Y (1) und Ln =Yb (2) sowie von [{2,6-(CH2C5H4)C5H3N}Y(III)(µ-OH)]2 (3)
[Y{2,6-(C5H4CH2)2C5H3N}(µ-OH)]2: a Dinuclear 5-ansa-Yttrocene Hydroxide containing the 2,6-dimethylenepyridyl Bridge
INORG. CHEM. COMM