The epidemiological type identification of Serratia marcescens from outbreaks of infection in hospitals

A study of serological, bacteriocine and phage typing of Serratia marcescens was made. Specific O-antisera of adequate titre were relatively simple to prepare but H-antisera exhibited many heterologous agglutination reactions amongst the type strains. Most of these cross-reactions were not reproduced when immobilization tests with H-sera were performed. Direct haemagglutination tests were used to establish the presence of fimbriae amongst the H-type strains and the results of agglutination tests with non-fimbriate variants of strains indicated that fimbrial antibody in high titre was present in some sera. Replicate typing of 100 pairs of cultures by the phage-typing method indicated that small variations in pattern were common and that larger variations occurred occasionally. Therefore differences in pattern of less than two strong reactions should not be taken as evidence that strains can be distinguished. Cultures of S. marcescens, 273 in total, from six outbreaks of infection in British and European hospitals were typed by O-serology, H agglutination and immobilization tests, phage typing and bacteriocine susceptibility by a cross-streaking method. The typability of strains by each method was high but the results suggested that no single method was sufficiently discriminating to be used alone for typing. Comparison of the H-type and typing patterns of members of the same O serogroup from incidents of infection showed that reliable results were obtained by H-typing or by phage and bacteriocine typing after the application of the appropriate ‘difference' rule. The greatest discrimination between strains of the same 0-group was obtained by the use of H-typing or phage typin

In search of ‘lost’ knowledge and outsourced expertise in flood risk management

This paper examines the parallel discourses of ‘lost’ local flood expertise and the growing use of commercial consultancies to outsource aspects of flood risk work. We critically examine the various claims and counter-claims about lost, local and external expertise in flood management, focusing on the aftermath of the 2007 floods in East Yorkshire, England. Drawing on interviews with consultants, drainage engineers and others, we caution against claims that privilege ‘local’ floods knowledge as ‘good’ and expert knowledge as somehow suspect. This paper urges carefulness in interpreting claims about local knowledge, arguing that it is important always to think instead of hybrid knowledge formations. We conclude by arguing that experiments in the co-production of flood risk knowledge need to be seen as part of a spectrum of ways for producing shared knowledge

Accuracy analysis of TDRSS demand forecasts

This paper reviews Space Network (SN) demand forecasting experience over the past 16 years and describes methods used in the forecasts. The paper focuses on the Single Access (SA) service, the most sought-after resource in the Space Network. Of the ten years of actual demand data available, only the last five years (1989 to 1993) were considered predictive due to the extensive impact of the Challenger accident of 1986. NASA's Space Network provides tracking and communications services to user spacecraft such as the Shuttle and the Hubble Space Telescope. Forecasting the customer requirements is essential to planning network resources and to establishing service commitments to future customers. The lead time to procure Tracking and Data Relay Satellites (TDRS's) requires demand forecasts ten years in the future a planning horizon beyond the funding commitments for missions to be supported. The long range forecasts are shown to have had a bias toward underestimation in the 1991 -1992 period. The trend of underestimation can be expected to be replaced by overestimation for a number of years starting with 1998. At that time demand from new missions slated for launch will be larger than the demand from ongoing missions, making the potential for delay the dominant factor. If the new missions appear as scheduled, the forecasts are likely to be moderately underestimated. The SN commitment to meet the negotiated customer's requirements calls for conservatism in the forecasting. Modification of the forecasting procedure to account for a delay bias is, therefore, not advised. Fine tuning the mission model to more accurately reflect the current actual demand is recommended as it may marginally improve the first year forecasting

Simulation of gain stability of THGEM gas-avalanche particle detectors

Charging-up processes affecting gain stability in Thick Gas Electron Multipliers (THGEM) were studied with a dedicated simulation toolkit. Integrated with Garfield++, it provides an effective platform for systematic phenomenological studies of charging-up processes in MPGD detectors. We describe the simulation tool and the fine-tuning of the step-size required for the algorithm convergence, in relation to physical parameters. Simulation results of gain stability over time in THGEM detectors are presented, exploring the role of electrode-thickness and applied voltage on its evolution. The results show that the total amount of irradiated charge through electrode's hole needed for reaching gain stabilization is in the range of tens to hundreds of pC, depending on the detector geometry and operational voltage. These results are in agreement with experimental observations presented previously

The Resistive-Plate WELL with Argon mixtures - a robust gaseous radiation detector

A thin single-element THGEM-based, Resistive-Plate WELL (RPWELL) detector was operated with 150 GeV/c muon and pion beams in Ne/(5%CH$_4$), Ar/(5%CH$_4$) and Ar/(7%CO$_2$); signals were recorded with 1 cm$^2$ square pads and SRS/APV25 electronics. Detection efficiency values greater than 98% were reached in all the gas mixtures, at average pad multiplicity of 1.2. The use of the 10$^9${\Omega}cm resistive plate resulted in a completely discharge-free operation also in intense pion beams. The efficiency remained essentially constant at 98-99% up to fluxes of $\sim$10$^4$Hz/cm$^2$, dropping by a few % when approaching 10$^5$ Hz/cm$^2$. These results pave the way towards cost-effective, robust, efficient, large-scale detectors for a variety of applications in future particle, astro-particle and applied fields. A potential target application is digital hadron calorimetry.Comment: presented at the 2016 VIenna Conf. On instrumentation. Submitted to the Conference proceeding

Proteolytic regulation of calcium channels - avoiding controversy

The publication of papers containing data obtained with suboptimal rigor in the experimental design and choice of key reagents, such as antibodies, can result in a lack of reproducibility and generate controversy that can both needlessly divert resources and, in some cases, damage public perception of the scientific enterprise. This exemplary paper by Buonarati et al. (2018)1 shows how a previously published, potentially important paper on calcium channel regulation falls short of the necessary mark, and aims to resolve the resulting controversy

Hydrodynamic dispersion within porous biofilms

Many microorganisms live within surface-associated consortia, termed biofilms, that can form intricate porous structures interspersed with a network of fluid channels. In such systems, transport phenomena, including flow and advection, regulate various aspects of cell behavior by controlling nutrient supply, evacuation of waste products, and permeation of antimicrobial agents. This study presents multiscale analysis of solute transport in these porous biofilms. We start our analysis with a channel-scale description of mass transport and use the method of volume averaging to derive a set of homogenized equations at the biofilm-scale in the case where the width of the channels is significantly smaller than the thickness of the biofilm. We show that solute transport may be described via two coupled partial differential equations or telegrapher's equations for the averaged concentrations. These models are particularly relevant for chemicals, such as some antimicrobial agents, that penetrate cell clusters very slowly. In most cases, especially for nutrients, solute penetration is faster, and transport can be described via an advection-dispersion equation. In this simpler case, the effective diffusion is characterized by a second-order tensor whose components depend on (1) the topology of the channels' network; (2) the solute's diffusion coefficients in the fluid and the cell clusters; (3) hydrodynamic dispersion effects; and (4) an additional dispersion term intrinsic to the two-phase configuration. Although solute transport in biofilms is commonly thought to be diffusion dominated, this analysis shows that hydrodynamic dispersion effects may significantly contribute to transport

First in-beam studies of a Resistive-Plate WELL gaseous multiplier

We present the results of the first in-beam studies of a medium size (10$\times$10 cm$^2$) Resistive-Plate WELL (RPWELL): a single-sided THGEM coupled to a pad anode through a resistive layer of high bulk resistivity ($\sim$10$^9 \Omega$cm). The 6.2~mm thick (excluding readout electronics) single-stage detector was studied with 150~GeV muons and pions. Signals were recorded from 1$\times$1 cm$^2$ square copper pads with APV25-SRS readout electronics. The single-element detector was operated in Ne\(5% $\mathrm{CH_{4}}$) at a gas gain of a few times 10$^4$, reaching 99$\%$ detection efficiency at average pad multiplicity of $\sim$1.2. Operation at particle fluxes up to $\sim$10$^4$ Hz/cm$^2$ resulted in $\sim$23$\%$ gain drop leading to $\sim$5$\%$ efficiency loss. The striking feature was the discharge-free operation, also in intense pion beams. These results pave the way towards robust, efficient large-scale detectors for applications requiring economic solutions at moderate spatial and energy resolutions.Comment: Accepted by JINS

Misdiagnosing Melioidosis

Melioidosis is endemic in southern and Southeast Asia and northern Australia. Although relatively few indigenous cases are recognized in the Indian subcontinent, a substantial proportion of cases imported into the United Kingdom originate there, probably reflecting patterns of immigration and travel, and underdiagnosis within the Indian subcontinent