The changing role of cell culture in the generation of transgenic livestock

Transgenesis may allow the generation of farm animals with altered phenotype, animal models for research and animal bioreactors. Although such animals have been produced, the time and expense involved in generating transgenic livestock and then evaluating the transgene expression pattern is very restrictive. If questions about the ability and efficiency of expression could be asked solely in vitro rapid progress could be achieved. Unfortunately, experiments addressing transcriptional control in vitro have proved unreliable in their ability to indicate whether a transgene will be transcribed or not. However, initial studies suggest that cell culture may be able to predict in vivo post-transcriptional events. We review these issues and propose that strategies which engineer the transgene integration site could enhance the probability for efficient expression. This approach has now become feasible with the development of techniques allowing animals to be generated from somatic cells by nuclear transfer. The important step in this procedure is the use of cells grown in culture as the source of genetic information, allowing the selection of specific transgene integration events. This technology which has dramatically increased the potential use of transgenic livestock for both agricultural and biotechnological applications, is based on standard cell culture methodology. We are now at the start of a new era in large animal transgenics

Application of the Contouring Method to Extended Microlensed Sources

The method devised by Lewis et al. (1993) for calculating the light curve of a microlensed point source is expanded to two dimensions to enable the calculation of light curves of extended sources. This method is significantly faster than the ray shooting method that has been used in the past. The increased efficiency is used to obtain much higher resolution light curves over increased timescales. We investigate the signatures arising from different source geometries in a realistic microlensing model. We show that a large fraction of high magnification events (HMEs) in image A of Q2237+0305 involve only one caustic, and could therefore yield information on the structure of the quasar continuum through the recognition of a characteristic event shape. In addition, the cataloguing of HMEs into morphological type will, in theory, enable the direction of the transverse motion, as well as the source size to be obtained from long term monitoring.Comment: 10 pages including 4 figures. Accepted for publication in M.N.R.A.

A measurement of the transverse velocity of Q2237+0305

Determination of microlensing parameters in the gravitationally lensed quasar Q2237+0305 from the statistics of high magnification events will require monitoring for more than 100 years (Wambsganss, Paczynski & Schneider 1990). However we show that the effective transverse velocity of the lensing galaxy can be determined on a more realistic time-scale through consideration of the distribution of light-curve derivatives. The 10 years of existing monitoring data for Q2237+0305 are analysed. These data display strong evidence for microlensing that is not associated with a high magnification event. An upper limit of v < 500 km/sec is obtained for the galactic transverse velocity which is smaller than previously assumed values. The analysis suggests that the observed microlensing variation may be predominantly due to stellar proper motions. The statistical significance of the results obtained from our method will be increased by the addition of data points from current and future monitoring campaigns. However reduced photometric errors will be more valuable than an increased sampling rate.Comment: 16 pages, including 17 figures. Accepted for publication in M.N.R.A.

Interpretation of the OGLE Q2237+0305 microlensing light-curve

The four bright images of the gravitationally lensed quasar Q2237+0305 are being monitored from the ground (eg. OGLE collaboration, Apache Point Observatory) in the hope of observing a high magnification event (HME). Over the past three seasons (1997-1999) the OGLE collaboration has produced microlensing light-curves with unprecedented coverage. These demonstrate smooth, independent (therefore microlensing) variability between the images (Wozniak et al. 2000a,b; OGLE web page). We have retrospectively compared probability functions for high-magnification event parameters with several observed light-curve features. We conclude that the 1999 image C peak was due to the source having passed outside of a cusp rather than to a caustic crossing. In addition, we find that the image C light-curve shows evidence for a caustic crossing between the 1997 and 1998 observing seasons involving the appearance of new critical images. Our models predict that the next image C event is most likely to arrive 500 days following the 1999 peak, but with a large uncertainty (100-2000 days). Finally, given the image A light-curve derivative at the end of the 1999 observing season, our modelling suggests that a caustic crossing will occur between the 1999 and 2000 observing seasons, implying a minimum for the image A light-curve ~1-1.5 magnitudes fainter than the November 1999 level.Comment: 11 pages, 15 figures. Accepted for publication in M.N.R.A.

Limits on the microlens mass function of Q2237+0305

Gravitational microlensing at cosmological distances is potentially a powerful tool for probing the mass functions of stars and compact objects in other galaxies. In the case of multiply-imaged quasars, microlensing data has been used to determine the average microlens mass. However the measurements have relied on an assumed transverse velocity for the lensing galaxy. Since the measured mass scales with the square of the transverse velocity, published mass limits are quite uncertain. In the case of Q2237+0305 we have properly constrained this uncertainty. The distribution of light curve derivatives allows quantitative treatment of the relative rates of microlensing due to proper motions of microlenses, the orbital stream motion of microlenses and the bulk galactic transverse velocity. By demanding that the microlensing rate due to the motions of microlenses is the minimum that should be observed we determine lower limits for the average mass of stars and compact objects in the bulge of Q2237+0305. If microlenses are assumed to move in an orbital stream the lower limit ranges between 0.005 and 0.023 solar masses where the the systematic dependence is due to the fraction of smooth matter and the size of photometric error assumed for published monitoring data. However, if the microlenses are assumed to move according to an isotropic velocity dispersion then a larger lower limit of 0.019-0.11 solar masses is obtained. A significant contribution of Jupiter mass compact objects to the mass distribution of the galactic bulge of Q2237+0305 is therefore unambiguously ruled out.Comment: 10 pages, 5 figures. Accepted for publication in Monthly Notices of the Royal Astronomical Society. New version has improved presentatio

Predicting caustic crossing high magnification events in Q2237+0305

The central regions of the gravitationally lensed quasar Q2237+0305 can be indirectly resolved on nano-arcsecond scales if viewed spectrophotometricly during a microlensing high magnification event (HME). Q2237+0305 is currently being monitored from the ground (eg. OGLE collaboration, Apache Point Observatory), with the goal, among others, of triggering ground and spacecraft based target of opportunity (TOO) observations of an HME. In this work we investigate the rate of change (trigger) in image brightness that signals an imminent HME and importantly, the separation between the trigger and the event peak. In addition, we produce colour dependent model light-curves by combining high-resolution microlensing simulations with a realistic model for a thermal accretion disc source. We make hypothetical target of opportunity spectroscopic observations using our determination of the appropriate trigger as a guide. We find that if the source spectrum varies with source radius, a 3 observation TOO program should be able to observe a microlensing change in the continuum slope following a light-curve trigger with a success rate of >80%.Comment: 17 pages, 16 figures, accepted for publication in M.N.R.A.

Determination and impact of surface radiative processes for TOGA COARE

Experiments using atmospheric general circulation models have shown that the atmospheric circulation is very sensitive to small changes in sea surface temperature in the tropical western Pacific Ocean warm pool region. The mutual sensitivity of the ocean and the atmosphere in the warm pool region places stringent requirements on models of the coupled ocean atmosphere system. At present, the situation is such that diagnostic studies using available data sets have been unable to balance the surface energy budget in the warm pool region to better than 50 to 80 W/sq m. The Tropical Ocean Global Atmosphere (TOGA) Coupled Ocean Atmosphere Response Experiment (COARE) is an observation and modelling program that aims specifically at the elucidation of the physical process which determine the mean and transient state of the warm pool region and the manner in which the warm pool interacts with the global ocean and atmosphere. This project focuses on one very important aspect of the ocean atmosphere interface component of TOGA COARE, namely the temporal and spatial variability of surface radiative fluxes in the warm pool region

Familiarity affects social network structure and discovery of prey patch locations in foraging stickleback shoals

Numerous factors affect the fine-scale social structure of animal groups, but it is unclear how important such factors are in determining how individuals encounter resources. Familiarity affects shoal choice and structure in many social fishes. Here, we show that familiarity between shoal members of sticklebacks (Gasterosteus aculeatus) affects both fine-scale social organization and the discovery of resources. Social network analysis revealed that sticklebacks remained closer to familiar than to unfamiliar individuals within the same shoal. Network-based diffusion analysis revealed that there was a strong untransmitted social effect on patch discovery, with individuals tending to discover a task sooner if a familiar individual from their group had previously done so than if an unfamiliar fish had done so. However, in contrast to the effect of familiarity, the frequency with which individuals had previously associated with one another had no effect upon the likelihood of prey patch discovery. This may have been due to the influence of fish on one another's movements; the effect of familiarity on discovery of an empty ‘control’ patch was as strong as for discovery of an actual prey patch. Our results demonstrate that factors affecting fine-scale social interactions can also influence how individuals encounter and exploit resources.Publisher PDFPeer reviewe