3,864 research outputs found
Hydrodynamic design of generic pump components
Inducer and impellar base geometries were defined for a fuel pump for a generic generator cycle. Blade surface data and inlet flowfield definition are available in sufficient detail to allow computational fluid dynamic analysis of the two components
Wrangling environmental exposure data: guidance for getting the best information from your laboratory measurements.
BACKGROUND:Environmental health and exposure researchers can improve the quality and interpretation of their chemical measurement data, avoid spurious results, and improve analytical protocols for new chemicals by closely examining lab and field quality control (QC) data. Reporting QC data along with chemical measurements in biological and environmental samples allows readers to evaluate data quality and appropriate uses of the data (e.g., for comparison to other exposure studies, association with health outcomes, use in regulatory decision-making). However many studies do not adequately describe or interpret QC assessments in publications, leaving readers uncertain about the level of confidence in the reported data. One potential barrier to both QC implementation and reporting is that guidance on how to integrate and interpret QC assessments is often fragmented and difficult to find, with no centralized repository or summary. In addition, existing documents are typically written for regulatory scientists rather than environmental health researchers, who may have little or no experience in analytical chemistry. OBJECTIVES:We discuss approaches for implementing quality assurance/quality control (QA/QC) in environmental exposure measurement projects and describe our process for interpreting QC results and drawing conclusions about data validity. DISCUSSION:Our methods build upon existing guidance and years of practical experience collecting exposure data and analyzing it in collaboration with contract and university laboratories, as well as the Centers for Disease Control and Prevention. With real examples from our data, we demonstrate problems that would not have come to light had we not engaged with our QC data and incorporated field QC samples in our study design. Our approach focuses on descriptive analyses and data visualizations that have been compatible with diverse exposure studies with sample sizes ranging from tens to hundreds of samples. Future work could incorporate additional statistically grounded methods for larger datasets with more QC samples. CONCLUSIONS:This guidance, along with example table shells, graphics, and some sample R code, provides a useful set of tools for getting the best information from valuable environmental exposure datasets and enabling valid comparison and synthesis of exposure data across studies
Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants
The crystallization of three catalytically inactive mutants of penicillin Vacylase (PVA) from Bacillus sphaericus in precursor and processed forms is reported. The mutant proteins crystallize in different primitive monoclinic space groups that are distinct from the crystal forms for the native enzyme. Directed mutants and clone constructs were designed to study the post-translational autoproteolytic processing of PVA. The catalytically inactive mutants will provide threedimensional structures of precursor PVA forms, plus open a route to the study of enzyme-substrate complexes for this industrially important enzyme
Expression, purification, crystallization and preliminary X-ray diffraction analysis of conjugated bile salt hydrolase from Bifidobacterium longum
Conjugated bile salt hydrolase (BSH) catalyses the hydrolysis of the amide bond that conjugates bile acids to glycine and to taurine. The BSH enzyme from Bifidobacterium longum was overexpressed in Escherichia coli BL21(DE3), purified and crystallized. Crystallization conditions were screened using the hanging-drop vapour-diffusion method. Crystal growth, with two distinct morphologies, was optimal in experiments carried out at 303 K. The crystals belong to the hexagonal system, space group P622 with unit-cell parameters a = b = 124.86, c = 219.03 Angstrom, and the trigonal space group P321, with unit-cell parameters a = b = 125.24, c = 117.03 Angstrom. The crystals diffracted X-rays to 2.5 Angstrom spacing. Structure determination using the multiple isomorphous replacement method is in progress
Expression, purification, crystallization and preliminary X-ray diffraction analysis of conjugated bile salt hydrolase from Bifidobacterium longum
Conjugated bile salt hydrolase (BSH) catalyses the hydrolysis of the amide bond that conjugates bile acids to glycine and to taurine. The BSH enzyme from Bifidobacterium longum was overexpressed in Escherichia coli BL21(DE3), purified and crystallized. Crystallization conditions were screened using the hanging-drop vapour-diffusion method. Crystal growth, with two distinct morphologies, was optimal in experiments carried out at 303 K. The crystals belong to the hexagonal system, space group P622 with unit-cell parameters a = b = 124.86, c = 219.03 Angstrom, and the trigonal space group P321, with unit-cell parameters a = b = 125.24, c = 117.03 Angstrom. The crystals diffracted X-rays to 2.5 Angstrom spacing. Structure determination using the multiple isomorphous replacement method is in progress
Saturn Forms by Core Accretion in 3.4 Myr
We present two new in situ core accretion simulations of Saturn with planet
formation timescales of 3.37 Myr (model S0) and 3.48 Myr (model S1), consistent
with observed protostellar disk lifetimes. In model S0, we assume rapid grain
settling reduces opacity due to grains from full interstellar values (Podolak
2003). In model S1, we do not invoke grain settling, instead assigning full
interstellar opacities to grains in the envelope. Surprisingly, the two models
produce nearly identical formation timescales and core/atmosphere mass ratios.
We therefore observe a new manifestation of core accretion theory: at large
heliocentric distances, the solid core growth rate (limited by Keplerian
orbital velocity) controls the planet formation timescale. We argue that this
paradigm should apply to Uranus and Neptune as well.Comment: 4 pages, including 1 figure, submitted to ApJ Letter
Deformation of continental crust along a transform boundary, Coast Mountains, British Columbia
New structural, paleomagnetic, and apatite (U-Th)/He results from the continental margin inboard of the Queen Charlotte fault (~54Ā°N) delineate patterns of brittle faulting linked to transform development since ~50 Ma. In the core of the orogen, ~250 km from the transform, north striking, dip-slip brittle faults and vertical axis rotation of large crustal domains occurred after ~50 Ma and before intrusion of mafic dikes at 20 Ma. By 20 Ma, dextral faulting was active in the core of the orogen, but extension had migrated toward the transform, continuing there until <9 Ma. Local tilting in the core of the orogen is associated with glacially driven, post-4 Ma exhumation. Integration with previous results shows that post-50 Ma dextral and normal faulting affected a region ~250 km inboard of the transform and ~300 km along strike. Initially widespread, the zone of active extension narrowed and migrated toward the transform ~25 Ma after initiation of the transform, while dextral faulting continued throughout the region. Differential amounts of post-50 Ma extension created oroclines at the southern and northern boundaries of the deformed region. This region approximately corresponds to continental crust that was highly extended just prior to transform initiation. Variation in Neogene crustal tilts weakens interpretations relying on uniform tilting to explain anomalous paleomagnetic inclinations of mid-Cretaceous plutons. Similarities to the Gulf of California suggest that development of a transform in continental crust is aided by previous crustal extension and that initially widespread extension narrows and moves toward the transform as the margin develops
Physical properties of high-mass clumps in different stages of evolution
(Abridged) Aims. To investigate the first stages of the process of high-mass
star formation, we selected a sample of massive clumps previously observed with
the SEST at 1.2 mm and with the ATNF ATCA at 1.3 cm. We want to characterize
the physical conditions in such sources, and test whether their properties
depend on the evolutionary stage of the clump.
Methods. With ATCA we observed the selected sources in the NH3(1,1) and (2,2)
transitions and in the 22 GHz H2O maser line. Ammonia lines are a good
temperature probe that allow us to accurately determine the mass and the
column-, volume-, and surface densities of the clumps. We also collected all
data available to construct the spectral energy distribution of the individual
clumps and to determine if star formation is already occurring, through
observations of its most common signposts, thus putting constraints on the
evolutionary stage of the source. We fitted the spectral energy distribution
between 1.2 mm and 70 microns with a modified black body to derive the dust
temperature and independently determine the mass.
Results. The clumps are cold (T~10-30 K), massive (M~10^2-10^3 Mo), and dense
(n(H2)>~10^5 cm^-3) and they have high column densities (N(H2)~10^23 cm^-2).
All clumps appear to be potentially able to form high-mass stars. The most
massive clumps appear to be gravitationally unstable, if the only sources of
support against collapse are turbulence and thermal pressure, which possibly
indicates that the magnetic field is important in stabilizing them.
Conclusions. After investigating how the average properties depend on the
evolutionary phase of the source, we find that the temperature and central
density progressively increase with time. Sources likely hosting a ZAMS star
show a steeper radial dependence of the volume density and tend to be more
compact than starless clumps.Comment: Published in A&A, Vol. 556, A1
- ā¦