180 research outputs found
Subcellular distribution and expression of cofilin and ezrin in human colon adenocarcinoma cell lines with different metastatic potential
The dynamic reorganization of the actin cytoskeleton is regulated by a number of actin binding proteins (ABPs). Four human colon adenocarcinoma cell lines – parental and three selected sublines, which differ in motility and metastatic potential, were used to investigate the expression level and subcellular localization of selected ABPs. Our interest was focused on cofilin and ezrin. These proteins are essential for cell migration and adhesion. The data received for the three more motile adenocarcinoma sublines (EB3, 3LNLN, 5W) were compared with those obtained for the parental LS180 adenocarcinoma cells and fibroblastic NRK cells. Quantitative densitometric analysis and confocal fluorescence microscopy were used to examine the expression levels and subcellular distribution of the selected ABPs. Our data show distinct increase in the level of cofilin in adenocarcinoma cells accompanied by the reduction of inactive phosphorylated form of cofilin. In more motile cells, cofilin was accumulated at cellular periphery in co-localization with actin filaments. Furthemore, we indicated translocation of ezrin towards the cell periphery within more motile cells in comparison with NRK and parental adenocarcinoma cells
Prototype development of the Integral-Field unit for VIRUS
VIRUS is a planned integral-field instrument for the Hobby-Eberly Telescope
(HET). In order to achieve a large field-of-view and high grasp at reasonable
costs, the approach is to replicate integral-field units (IFU) and medium sized
spectrographs many times. The Astrophysical Institute Potsdam (AIP) contributes
to VIRUS with the development and testing of the IFU prototype. This paper
describes the optomechanical design and the manufacture of the fiber-based IFU
subsystem. The initial VIRUS development aims to produce a prototype and to
measure its performance. Additionally, techniques will be investigated to allow
industrial replication of the highly specific fiber-bundle layout. This will be
necessary if this technique is to be applied to the next generation of even
larger astronomical instrumentation.Comment: 11 pages, 13 figures, to be published in SPIE proc. 627
The GREGOR Fabry-P\'erot Interferometer
The GREGOR Fabry-P\'erot Interferometer (GFPI) is one of three first-light
instruments of the German 1.5-meter GREGOR solar telescope at the Observatorio
del Teide, Tenerife, Spain. The GFPI uses two tunable etalons in collimated
mounting. Thanks to its large-format, high-cadence CCD detectors with
sophisticated computer hard- and software it is capable of scanning spectral
lines with a cadence that is sufficient to capture the dynamic evolution of the
solar atmosphere. The field-of-view (FOV) of 50" x 38" is well suited for quiet
Sun and sunspot observations. However, in the vector spectropolarimetric mode
the FOV reduces to 25" x 38". The spectral coverage in the spectroscopic mode
extends from 530-860 nm with a theoretical spectral resolution R of about
250,000, whereas in the vector spectropolarimetric mode the wavelength range is
at present limited to 580-660 nm. The combination of fast narrow-band imaging
and post-factum image restoration has the potential for discovery science
concerning the dynamic Sun and its magnetic field at spatial scales down to
about 50 km on the solar surface.Comment: 14 pages, 17 figures, 4 tables; pre-print of AN 333, p.880-893, 2012
(AN special issue to GREGOR
A retrospective of the GREGOR solar telescope in scientific literature
In this review, we look back upon the literature, which had the GREGOR solar
telescope project as its subject including science cases, telescope subsystems,
and post-focus instruments. The articles date back to the year 2000, when the
initial concepts for a new solar telescope on Tenerife were first presented at
scientific meetings. This comprehensive bibliography contains literature until
the year 2012, i.e., the final stages of commissioning and science
verification. Taking stock of the various publications in peer-reviewed
journals and conference proceedings also provides the "historical" context for
the reference articles in this special issue of Astronomische
Nachrichten/Astronomical Notes.Comment: 6 pages, 2 color figures, this is the pre-peer reviewed version of
Denker et al. 2012, Astron. Nachr. 333, 81
Performance of the QuickVue Influenza A+B Rapid Test for Pandemic H1N1 (2009) Virus Infection in Adults
To investigate the diagnostic accuracy of the QuickVue® Influenza A+B rapid test we conducted a prospective observational study in which this rapid test was compared with a real-time reverse transcription polymerase chain reaction (RT-PCR) for pandemic influenza A H1N1 (2009) infection in Austrian adults. The sensitivity, specificity, and positive and negative predictive values of the QuickVue test compared with the RT-PCR were 26% (95% CI 18–35), 98% (95% CI 92–100), 94% (95% CI 80–99) and 50% (95% CI 42–58), respectively. The prevalence of pandemic H1N1 (2009) virus infection among the 209 patients included in the study was 57%. Our data suggest that a positive QuickVue test provides considerable information for the diagnosis of pandemic influenza A H1N1 (2009) virus infection in young adults but that a negative QuickVue test result should, if relevant for patient management or public health measures, be verified using PCR
Worldwide variations in artificial skyglow
Open access journalDespite constituting a widespread and significant environmental change, understanding of artificial nighttime skyglow is extremely limited. Until now, published monitoring studies have been local or regional in scope, and typically of short duration. In this first major international compilation of monitoring data we answer several key questions about skyglow properties. Skyglow is observed to vary over four orders of magnitude, a range hundreds of times larger than was the case before artificial light. Nearly all of the study sites were polluted by artificial light. A non-linear relationship is observed between the sky brightness on clear and overcast nights, with a change in behavior near the rural to urban landuse transition. Overcast skies ranged from a third darker to almost 18 times brighter than clear. Clear sky radiances estimated by the World Atlas of Artificial Night Sky Brightness were found to be overestimated by ~25%; our dataset will play an important role in the calibration and ground truthing of future skyglow models. Most of the brightly lit sites darkened as the night progressed, typically by ~5% per hour. The great variation in skyglow radiance observed from site-to-site and with changing meteorological conditions underlines the need for a long-term international monitoring program.MILIEU (FU Berlin)Federal Ministry of Education and Research, GermanyEU COST Action ES1204 (Loss of the Night Network)European Research Council (ERC) under the EU's Seventh Framework Program (FP7/2007-2013)panish Network for Light Pollution StudiesNational Aeronautics and Space Administration (Goddard Space Flight Center)Ohio State UniversityUniversity of IowaThe Adam Mickiewicz Universit
Worldwide variations in artificial skyglow
Despite constituting a widespread and significant environmental change,
understanding of artificial nighttime skyglow is extremely limited. Until now,
published monitoring studies have been local or regional in scope, and
typically of short duration. In this first major international compilation of
monitoring data we answer several key questions about skyglow properties.
Skyglow is observed to vary over four orders of magnitude, a range hundreds of
times larger than was the case before artificial light. Nearly all of the
study sites were polluted by artificial light. A non-linear relationship is
observed between the sky brightness on clear and overcast nights, with a
change in behavior near the rural to urban landuse transition. Overcast skies
ranged from a third darker to almost 18 times brighter than clear. Clear sky
radiances estimated by the World Atlas of Artificial Night Sky Brightness were
found to be overestimated by ~25%; our dataset will play an important role in
the calibration and ground truthing of future skyglow models. Most of the
brightly lit sites darkened as the night progressed, typically by ~5% per
hour. The great variation in skyglow radiance observed from site-to-site and
with changing meteorological conditions underlines the need for a long-term
international monitoring program
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