Aneuploidy among androgenic progeny of hexaploid triticale (XTriticosecale Wittmack).

Doubled haploids are an established tool in plant breeding and research. Of several methods for their production, androgenesis is technically simple and can efficiently produce substantial numbers of lines. It is well suited to such crops as hexaploid triticale. Owing to meiotic irregularities of triticale hybrids, aneuploidy may affect the efficiency of androgenesis more severely than in meiotically stable crops. This study addresses the issue of aneuploidy among androgenic regenerants of triticale. Plant morphology, seed set and seed quality were better predictors of aneuploidy, as determined cytologically, than flow cytometry. Most aneuploids were hypoploids and these included nullisomics, telosomics, and translocation lines; among 42 chromosome plants were nulli-tetrasomics. Rye chromosomes involved in aneuploidy greatly outnumbered wheat chromosomes; in C(0) rye chromosomes 2R and 5R were most frequently involved. While the frequency of nullisomy 2R was fairly constant in most cross combinations, nullisomy 5R was more frequent in the most recalcitrant combination, and its frequency increased with time spent in culture with up to 70% of green plants recovered late being nullisomic 5R. Given that 5R was not involved in meiotic aberrations with an above-average frequency, it is possible that its absence promotes androgenesis or green plant regeneration. Overall, aneuploidy among tested combinations reduced the average efficiency of double haploid production by 35% and by 69% in one recalcitrant combination, seriously reducing the yield of useful lines

Populations of doubled haploids for genetic mapping in hexaploid winter triticale.

To create a framework for genetic dissection of hexaploid triticale, six populations of doubled haploid (DH) lines were developed from pairwise hybrids of high-yielding winter triticale cultivars. The six populations comprise between 97 and 231 genotyped DH lines each, totaling 957 DH lines. A consensus genetic map spans 4593.9 cM is composed of 1576 unique DArT markers. The maps reveal several structural rearrangements in triticale genomes. In preliminary tests of the populations and maps, markers specific to wheat segments of the engineered rye chromosome 1R (RM1B) were identified. Example QTL mapping of days to heading in cv. Krakowiak revealed loci on chromosomes 2BL and 2R responsible for extended vernalization requirement, and candidate genes were identified. The material is available to all parties interested in triticale genetics

Exact renormalization group equations and the field theoretical approach to critical phenomena

After a brief presentation of the exact renormalization group equation, we illustrate how the field theoretical (perturbative) approach to critical phenomena takes place in the more general Wilson (nonperturbative) approach. Notions such as the continuum limit and the renormalizability and the presence of singularities in the perturbative series are discussed.Comment: 15 pages, 7 figures, to appear in the Proceedings of the 2nd Conference on the Exact Renormalization Group, Rome 200

Renormalization Group Flow Equations and the Phase Transition in O(N)-models

We derive and solve flow equations for a general O(N)-symmetric effective potential including wavefunction renormalization corrections combined with a heat-kernel regularization. We investigate the model at finite temperature and study the nature of the phase transition in detail. Beta functions, fixed points and critical exponents \beta, \nu, \delta and \eta for various N are independently calculated which allow for a verification of universal scaling relations.Comment: 34 pages, 3 tables, 11 postscript figures, LaTe

The expression of B7-H1 and B7-H4 molecules on immature myeloid and lymphoid dendritic cells in cord blood of healthy neonates.

The aim of our study was to estimate both B7-H1 and B7-H4 molecules on immature myeloid and lymphoid dendritic cells in umbilical cord blood of healthy neonates in comparison with peripheral blood of healthy adults. Thirty nine healthy full-term neonates from physiological single pregnancies and 27 healthy adults were included in the study. The expression of B7-H1 and B7-H4 was revealed using the immunofluorescence method. Statistical analysis was performed using a non-parametric test (Mann-Whitney U-Test). The percentages of BDCA-1+ dendritic cells with B7-H1 and B7-H4 expressions were significantly higher in peripheral blood of healthy adults (

The Phase Diagram of the Quark-Meson Model

Within the proper-time renormalization group approach, the chiral phase diagram of a two-flavor quark-meson model is studied. In the chiral limit, the location of the tricritical point which is linked to a Gaussian fixed point, is determined. For quark chemical potentials smaller than the tricritical one the second-order phase transition belongs to the O(4) universality class. For temperatures below the tricritical one we find initially a weak first-order phase transition which is commonly seen in model studies and also in recent lattice simulations. In addition, below temperatures of T <~ 17 MeV we find two phase transitions. The chiral restoration transition is initially also of first-order but turns into a second-order transition again. This leads to the possibility that there may be a "second tricritical" point in the QCD phase diagram in the chiral limit.Comment: 19 pages, 4 figures,v2; minor corrections in the text, references added, slightly extended conclusion, to appear in NP

On the Convergence of the Expansion of Renormalization Group Flow Equation

We compare and discuss the dependence of a polynomial truncation of the effective potential used to solve exact renormalization group flow equation for a model with fermionic interaction (linear sigma model) with a grid solution. The sensitivity of the results on the underlying cutoff function is discussed. We explore the validity of the expansion method for second and first-order phase transitions.Comment: 12 pages with 10 EPS figures included; revised versio

Completeness and consistency of renormalisation group flows

We study different renormalisation group flows for scale dependent effective actions, including exact and proper-time renormalisation group flows. These flows have a simple one loop structure. They differ in their dependence on the full field-dependent propagator, which is linear for exact flows. We investigate the inherent approximations of flows with a non-linear dependence on the propagator. We check explicitly that standard perturbation theory is not reproduced. We explain the origin of the discrepancy by providing links to exact flows both in closed expressions and in given approximations. We show that proper-time flows are approximations to Callan-Symanzik flows. Within a background field formalism, we provide a generalised proper-time flow, which is exact. Implications of these findings are discussed.Comment: 33 pages, 15 figures, revtex, typos corrected, to be published in Phys.Rev.

Perturbative and non-perturbative aspects of the proper time renormalization group

The renormalization group flow equation obtained by means of a proper time regulator is used to calculate the two loop beta function and anomalous dimension eta of the field for the O(N) symmetric scalar theory. The standard perturbative analysis of the flow equation does not yield the correct results for both beta and eta. We also show that it is still possible to extract the correct beta and eta from the flow equation in a particular limit of the infrared scale. A modification of the derivation of the Exact Renormalization Group flow, which involves a more general class of regulators, to recover the proper time renormalization group flow is analyzed.Comment: 26 pages.Latex.Version accepted for publicatio