Somatic embryogenesis from zygotic embryos and thin cell layers (TCLs) of Brazilian oil palm (Elaeis guineensis × Elaeis oleifera)

Oil palm hybrid BRS Manicoré is important for plantations in the north of Brazil, as it is resistant to fatal yellowing and is compact. Seed germination is slow and reduced, so somatic embryogenesis is a promising alternative for its propagation. Two kinds of starting explants were used: Zygotic embryos (ZE) and thin cell layers (TCL) from the base of seedling aerial parts. Two culture medium formulae were compared (Y3 and modified MS), and several growth regulators (2,4-D, Picloram, BA, 2iP),  putrescine and activated charcoal (AC) were used during the different stages. Somatic embryos (SE) were obtained from ZE-derived embryogenic masses cultured on Y3 medium with or without 2,4-D (9 μM) + 1000 μM putrescine and 40% were converted into seedlings in the Y3 medium supplemented with 2 g.L-¹ AC and without growth regulators, under light. SE developed in 49 to 53% TCL-derived calli and 50% of them were converted into seedlings in the same conditions described for ZE calli. The yield of both processes was compared, showing the superiority of TCL explants for SE production.Key words: Y3 culture medium, clonal propagation, putrescine, thin cell layer

STRATEGI GURU MENGAJARI PERILAKU BERKOMITMEN SISWA KELAS X SMK HASAEL PURU DALAM PEMBELAJARAN PENJASORKES

This research is to describe the strategy of the teacher in behavior commitment teaching by students X vocational high school (SMK) Hasael puru on physical education learning process. There is the aim of study is to explain the strategy of teacher as the eduator on behavior commitment teaching by students physical sport education learning process. The method that used in this research is qualitative method, the technique which is used to collected the data is triangulation technique there are: documentation, interview,observation.the steps of analysing data are: reduction data, display data and verification. The triangulation data and the triangulation technique are used to test the validity of the data. The result of the study express about 6 findings there are : 1. Behavior commitment teaching, 2. The agreement and the aim of behavior  commitment teaching, 3. Physical sport education learning process is to form the behavior commitment, 4. The strategy of behavior commitment teaching, 5. The manner to develop the behavior of commitment, 6. What kind of the characteric that be supposed from behavior commitment teachingPenelitian ini untuk mendeskripsikan Strategi Guru Mengajari Perilaku Berkomitmen Siswa kelas X SMK Hasael Puru Dalam Pembelajaran Penjasorkes. Adapun tujuan penelitian ini untuk menjelaskan strategi guru penjasorkes sebagai pendidik dalam mengajari perilaku berkomitmen siswa dalam pembelajaran penjasorkes.Metode yang digunakan adalah jenis kualitatif, dengan pengumpulan data penelitian diperoleh dengan teknik tringulasi dengan teknik pengumpulan data : dokumentasi, wawancara, dan observasi. Langkah menganalisis data adalah dengan data reduction (reduksi data), data display dan verivication. Untuk menguji keabsahan data dilakukan dengan menggunakan triangulasi data dan triangulasi metode. Hasil Penelitian ini mengungkapkan lima temuan yaitu: (1) Mengajari Perilaku Berkomitmen. (2) Adanya perjanjian dan tujuan dalam mengajari perilaku berkomitmen (3) Pembelajaran Penjasorkes membentuk perilaku berkomitmen. (4) Strategi dalam Mengajari Perilaku Berkomitmen. (5) Cara Menumbuhkan Perilaku Berkomitmen. (6) Karakter Seperti apa yang diharapkan dari Mengajari Perilaku Berkomitmen

Technology-Based Communication and the Development of Interpersonal Competencies Within Adolescent Romantic Relationships: A Preliminary Investigation

This study investigated longitudinal associations between adolescents’ technology-based communication and the development of interpersonal competencies within romantic relationships. A school-based sample of 487 adolescents (58% girls; Mage = 14.1) participated at two time points, one year apart. Participants reported (1) proportions of daily communication with romantic partners via traditional modes (in person, on the phone) versus technological modes (text messaging, social networking sites) and (2) competence in the romantic relationship skill domains of negative assertion and conflict management. Results of cross-lagged panel models indicated that adolescents who engaged in greater proportions of technology-based communication with romantic partners reported lower levels of interpersonal competencies one year later, but not vice versa; associations were particularly strong for boys

Uso de PPM® (Plant Preservative Mixture) na desinfestação de sementes de guanandi (Calophyllum brasiliense Cambess.), introduzidas in vitro.

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Uso de antioxidantes no estabelecimento in vitro de segmentos nodais de guanandi (Calophyllum brasiliense Cambess.).

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Dipole and Bloch oscillations of cold atoms in a parabolic lattice

The paper studies the dynamics of a Bose-Einstein condensate loaded into a 1D parabolic optical lattice, and excited by a sudden shift of the lattice center. Depending on the magnitude of the initial shift, the condensate undergoes either dipole or Bloch oscillations. The effects of dephasing and of atom-atom interactions on these oscillations are discussed.Comment: 3 pages, to appear in proceeding of LPHYS'05 conference (July 4-8, 2005, Kyoto, Japan

Produção orgânica de diferentes cultivares de morangueiro cultivados em solo coberto com acículas de pínus e plástico preto na região de Chapecó, SC.

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Regulation of FATTY ACID ELONGATION1 expression in embryonic and vascular tissues of Brassica napus

International audienceThe expression of the FATTY ACID ELONGATION1 genes was characterised to provide insight into the regulation of very long chain fatty acid (VLCFA) biosynthesis in Brassica napus embryos. Each of the two rapeseed homoeologous genes (Bn-FAE1.1 and Bn-FAE1.2) encoding isozymes of 3-keto-acylCoA synthase, a subunit of the cytoplasmic acyl-CoA elongase complex that controls the production of elongated fatty acids, are expressed predominantly in developing seeds. The proximal regions of the Bn-FAE1.1 and Bn-FAE1.2 promoters possess strong sequence identity suggesting that transcriptional control of expression is mediated by this region which contains putative cis-elements characteristic of those found in the promoters of genes expressed in embryo and endosperm. Histochemical staining of rapeseed lines expressing Bn-FAE1.1 promoter:reporter gene fusions revealed a strong expression in the embryo cotyledon and axis throughout the maturation phase. Quantitative analyses revealed the region, −331 to −149, exerts a major control on cotyledon specific expression and the level of expression. A second region, −640 to −475, acts positively to enhance expression levels and extends expression of Bn-FAE1.1 into the axis and hypocotyl but also acts negatively to repress expression in the root meristem. The expression of the Bn-FAE1.1 gene was not restricted to the seed but was also detected in the vascular tissues of germinating seedlings and mature plants in the fascicular cambium tissue present in roots, stem and leaf petiole. We propose that Bn-FAE1.1 expression in vascular tissue may contribute VLCFA for barrier lipid synthesis and reflects the ancestral function of FAE1 encoded 3-keto-acylCoA synthase

Evaluation of a pilot consultation for maternity protection at work in Switzerland.

Switzerland's Labour Law and its Ordonnance on Maternity Protection aim to protect the health of pregnant employees and their unborn children while enabling them to continue to pursue their professional activities. Some companies encounter difficulties implementing the law's provisions. The Department of Occupational and Environmental Health, part of the Center for Primary Care and Public Health (Unisanté), has provided specialist occupational medicine consultations for pregnant employees since 2015. This study aimed to evaluate how well Swiss' maternity protection legislation is implemented by examining a list of relevant indicators measured during the occupational health consultation. The study also sought to investigate the consultation support provided to the relevant stakeholders and the adjustments made to pregnant employees' working conditions. Descriptive variables and indicators relative to the application of the Swiss maternity protection legislation for 83 pregnant employees were collected during the consultation's pilot phase (between 2015 and 2016). Descriptive statistics and cross-analyses of these indicators were made. Most pregnant employees faced multiple exposures to occupational risks. Preventive risk analyses were rare. Few adjustments to workstations were proposed. We found a tendency for employees to leave their workstations early on in their pregnancies due to sick leave certificate prescriptions. Specialist consultation and collaboration with occupational health physicians to recommend interventions for pregnant employees can provide significant benefits and help some pregnant women to continue at their workstations with appropriate adjustments. A specialised occupational health consultation is a useful instrument for identifying occupational hazards for both the pregnant woman and her unborn child. It is also an opportunity to explain employers' legal responsibilities and obligations to safeguard the health of their pregnant employees and to give specific advice for their company's situation. This consultation also enables employers to maintain their employees' valuable professional competencies in the workplace for as long as possible. Finally, occupational health consultation helps and supports healthcare providers who must, according to the law, make decisions about whether pregnant employees can continue working safely or not