Regions of the T cell receptor alpha and beta chains that are responsible for interactions with CD3.

The T cell antigen receptor consists of the Ti alpha/beta heterodimer which recognizes antigen, and the associated CD3 chains, thought to be involved in signal transduction. To understand the nature of the interaction between Ti and CD3, chimeric molecules which included the COOH-terminal segments of Ti alpha or beta linked to the extracellular segment of CD8, were transfected into a mutant T cell deficient in Ti beta chain expression and cell surface CD3. Both chimeric chains were required to express the chimeric Ti and to restore CD3 surface expression. CD8/Ti and CD3 cointernalized and coimmunoprecipitated. Stimulation of the chimeric receptor induced transmembrane signaling events and cell activation. These results demonstrate that the Ti alpha and beta COOH termini containing the transmembrane domains are sufficient for structural and functional coupling of Ti to CD3

Optical Properties of Quantum-Dot-Doped Liquid Scintillators

Semiconductor nanoparticles (quantum dots) were studied in the context of liquid scintillator development for upcoming neutrino experiments. The unique optical and chemical properties of quantum dots are particularly promising for the use in neutrinoless double beta decay experiments. Liquid scintillators for large scale neutrino detectors have to meet specific requirements which are reviewed, highlighting the peculiarities of quantum-dot-doping. In this paper, we report results on laboratory-scale measurements of the attenuation length and the fluorescence properties of three commercial quantum dot samples. The results include absorbance and emission stability measurements, improvement in transparency due to filtering of the quantum dot samples, precipitation tests to isolate the quantum dots from solution and energy transfer studies with quantum dots and the fluorophore PPO.Comment: version 2, minor text update

On integrability of the differential constraints arising from the singularity analysis

Integrability of the differential constraints arising from the singularity analysis of two (1+1)-dimensional second-order evolution equations is studied. Two nonlinear ordinary differential equations are obtained in this way, which are integrable by quadratures in spite of very complicated branching of their solutions.Comment: arxiv version is already offcia

Baffling of fluid sloshing in cylindrical tanks Final report

Annular baffle for damping liquid oscillations in partially filled cylindrical tan

Identification of Coulomb blockade and macroscopic quantum tunneling by noise

The effects of Macroscopic Quantum Tunneling (MQT) and Coulomb Blockade (CB) in Josephson junctions are of considerable significance both for the manifestations of quantum mechanics on the macroscopic scale and potential technological applications. These two complementary effects are shown to be clearly distinguishable from the associated noise spectra. The current noise is determined exactly and a rather sharp crossover between flux noise in the MQT and charge noise in the CB regions is found as the applied voltage is changed. Related results hold for the voltage noise in current-biased junctions.Comment: 6 pages, 3 figures, epl.cls include

System characterization of positive real conditions

Necessary and sufficient conditions for positive realness in terms of state space matrices are presented under the assumption of complete controllability and complete observability of square systems with independent inputs. As an alternative to the positive real lemma and to the s-domain inequalities, these conditions provide a recursive algorithm for testing positive realness which result in a set of simple algebraic conditions. By relating the positive real property to the associated variational problem, a unified derivation of necessary and sufficient conditions for optimality of both singular and nonsingular problems is derived

Monitoring the Impact of Health Reform on Americans 50-64: Medicaid Expansion and Marketplace Implementation Increased Health Coverage

This survey shows that the share of 50- to 64-year-olds without health insurance fell between December 2013 and March 2014. In states that expanded their Medicaid programs, a greater share of previously uninsured adults gained coverage, particularly among groups that have traditionally faced barriers to obtaining it. The survey also found that the newly insured differed in key ways from those who reported being insured for all of the past 12 months. On average, more were low income, and more reported that they had had trouble paying medical bills. This paper is part of a series that looks at the experience of 50- to 64-year-olds during the first open enrollment period of the Affordable Care Act (ACA)

Finger extensor variability in TMS parameters among chronic stroke patients

BACKGROUND: This study determined the reliability of topographic motor cortical maps and MEP characteristics in the extensor digitorum communis (EDC) evoked by single-pulse TMS among patients with chronic stroke. METHODS: Each of ten patients was studied on three occasions. Measures included location of the EDC hotspot and center of gravity (COG), threshold of activation and average amplitude of the hotspot, number of active sites, map volume, and recruitment curve (RC) slope. RESULTS: Consistent intrahemispheric measurements were obtained for the three TMS mapping sessions for all measured variables. No statistically significant difference was observed between hemispheres for the number of active sites, COG distance or the RC slope. The magnitude and range of COG movement between sessions were similar to those reported previously with this muscle in able-bodied individuals. The average COG movement over three sessions in both hemispheres was 0.90 cm. The average COG movement in the affected hemisphere was 1.13 (± 0.08) cm, and 0.68 (± 0.04) cm) for the less affected hemisphere. However, significant interhemispheric variability was seen for the average MEP amplitude, normalized map volume, and resting motor threshold. CONCLUSION: The physiologic variability in some TMS measurements of EDC suggest that interpretation of TMS mapping data derived from hemiparetic patients in the chronic stage following stroke should be undertaken cautiously. Irrespective of the muscle, potential causes of variability should be resolved to accurately assess the impact of pharmacological or physical interventions on cortical organization as measured by TMS among patients with stroke

Sequence of the mouse Q4 class I gene and characterization of the gene product

The Q4 class I gene has been shown to participate in gene conversion events within the mouse major histocompatibility complex. Its complete genomic nucleotide sequence has been determined. The 5' half of Q4 resembles H-2 genes more strongly than other Q genes. Its 3' end, in contrast, is Q-like and contains a translational stop signal in exon 5 which predicts a polypeptide with an incomplete membrane spanning segment. The presence of two inverted B1 repeats suggests that part of the Q4 gene may be mobile within the genome. Gene transfer experiments have shown that the Q4 gene encodes a ß2-microglobulin associated polypeptide of Mr 41 000. A similar protein was found in activated mouse spleen cells. The Q4 polypeptide was found to be secreted both by spleen cells and by transfected fibroblasts and was not detectable on the cell surface. Antibody binding and twodimensional gel electrophoresis indicate that the Q4 molecule is identical to a mouse class I polypeptide, Qb-1, which has been previously described

Magnetic buoyancy instabilities in the presence of magnetic flux pumping at the base of the solar convection zone

We perform idealized numerical simulations of magnetic buoyancy instabilities in three dimensions, solving the equations of compressible magnetohydrodynamics in a model of the solar tachocline. In particular, we study the effects of including a highly simplified model of magnetic flux pumping in an upper layer (‘the convection zone’) on magnetic buoyancy instabilities in a lower layer (‘the upper parts of the radiative interior – including the tachocline’), to study these competing flux transport mechanisms at the base of the convection zone. The results of the inclusion of this effect in numerical simulations of the buoyancy instability of both a preconceived magnetic slab and a shear-generated magnetic layer are presented. In the former, we find that if we are in the regime that the downward pumping velocity is comparable with the Alfvén speed of the magnetic layer, magnetic flux pumping is able to hold back the bulk of the magnetic field, with only small pockets of strong field able to rise into the upper layer. In simulations in which the magnetic layer is generated by shear, we find that the shear velocity is not necessarily required to exceed that of the pumping (therefore the kinetic energy of the shear is not required to exceed that of the overlying convection) for strong localized pockets of magnetic field to be produced which can rise into the upper layer. This is because magnetic flux pumping acts to store the field below the interface, allowing it to be amplified both by the shear and by vortical fluid motions, until pockets of field can achieve sufficient strength to rise into the upper layer. In addition, we find that the interface between the two layers is a natural location for the production of strong vertical gradients in the magnetic field. If these gradients are sufficiently strong to allow the development of magnetic buoyancy instabilities, strong shear is not necessarily required to drive them (cf. previous work by Vasil & Brummell). We find that the addition of magnetic flux pumping appears to be able to assist shear-driven magnetic buoyancy in producing strong flux concentrations that can rise up into the convection zone from the radiative interior