529 research outputs found
Rasamsonia, a new genus comprising thermotolerant and thermophilic Talaromyces and Geosmithia species
The phylogenetic relationship among Geosmithia argillacea, Talaromyces emersonii, Talaromyces byssochlamydoides and other members of the Trichocomaceae was studied using partial RPB2 (RNA polymerase II gene, encoding the second largest protein subunit), Tsr1 (putative ribosome biogenesis protein) and Cct8 (putative chaperonin complex component TCP-1) gene sequences. The results showed that these species form a distinct clade within the Trichocomaceae and Trichocoma paradoxa is phylogenetically most closely related. Based on phenotypic and physiological characters and molecular data, we propose Rasamsonia gen. nov. to accommodate these species. This new genus is distinct from other genera of the Trichocomaceae in being thermotolerant or thermophilic and having conidiophores with distinctly rough walled stipes, olive-brown conidia and ascomata, if present, with a scanty covering. Species within the genus Rasamsonia were distinguished using a combination of phenotypic characters, extrolite patterns, ITS and partial calmodulin and β-tubulin sequences. Rasamsonia brevistipitata sp. nov. is described and five new combinations are proposed
Taxonomy of Penicillium section Citrina
Species of Penicillium section Citrina have a worldwide distribution and occur commonly in soils. The section is here delimited using a combination of phenotypic characters and sequences of the nuclear ribosomal RNA gene operon, including the internal transcribed spacer regions ITS1 and ITS2, the 5.8S nrDNA (ITS) and partial RPB2 sequences. Species assigned to section Citrina share the production of symmetrically biverticillate conidiophores, flask shaped phialides (7.0–9.0 μm long) and relatively small conidia (2.0–3.0 μm diam). Some species can produce greyish-brown coloured cleistothecia containing flanged ascospores. In the present study, more than 250 isolates presumably belonging to section Citrina were examined using a combined analysis of phenotypic and physiological characters, extrolite profiles and ITS, β-tubulin and/or calmodulin sequences. Section Citrina includes 39 species, and 17 of those are described here as new. The most important phenotypic characters for distinguishing species are growth rates and colony reverse colours on the agar media CYA, MEA and YES; shape, size and ornamentation of conidia and the production of sclerotia or cleistothecia. Temperature-growth profiles were made for all examined species and are a valuable character characters for species identification. Species centered around P. citrinum generally have a higher maximum growth temperature (33–36 °C) than species related to P. westlingii (27–33 °C). Extrolite patterns and partial calmodulin and β-tubulin sequences can be used for sequence based identification and resolved all species. In contrast, ITS sequences were less variable and only 55 % of the species could be unambiguously identified with this locus
Polyphasic taxonomy of the heat resistant ascomycete genus Byssochlamys and its Paecilomyces anamorphs
Byssochlamys and related Paecilomyces strains are often heat resistant and may produce mycotoxins in contaminated pasteurised foodstuffs. A comparative study of all Byssochlamys species was carried out using a polyphasic approach to find characters that differentiate species and to establish accurate data on potential mycotoxin production by each species. Phylogenetic analysis of the ITS region, parts of the β-tubulin and calmodulin genes, macro- and micromorphological examinations and analysis of extrolite profiles were applied. Phylogenetic analyses revealed that the genus Byssochlamys includes nine species, five of which form a teleomorph, i.e. B. fulva, B. lagunculariae, B. nivea, B. spectabilis and B. zollerniae, while four are asexual, namely P. brunneolus, P. divaricatus, P. formosus and P. saturatus. Among these, B. nivea produces the mycotoxins patulin and byssochlamic acid and the immunosuppressant mycophenolic acid. Byssochlamys lagunculariae produces byssochlamic acid and mycophenolic acid and thus chemically resembles B. nivea. Some strains of P. saturatus produce patulin and brefeldin A, while B. spectabilis (anamorph P. variotii s.s.) produces viriditoxin. Some micro- and macromorphological characters are valuable for identification purposes, including the shape and size of conidia and ascospores, presence and ornamentation of chlamydospores, growth rates on MEA and CYA and acid production on CREA. A dichotomous key is provided for species identification based on phenotypical characters
Taxonomic revision of Aspergillus section Clavati based on molecular, morphological and physiological data
Aspergillus section Clavati has been revised using
morphology, secondary metabolites, physiological characters and DNA sequences.
Phylogenetic analysis of β-tubulin, ITS and calmodulin sequence data
indicated that Aspergillus section Clavati includes 6
species, A. clavatus (synonyms: A. apicalis, A.
pallidus), A. giganteus, A. rhizopodus, A.
longivesica, Neocarpenteles acanthosporus and A. clavatonanicus.
Neocarpenteles acanthosporus is the only known teleomorph of this
section. The sister genera to Neocarpenteles are Neosartorya
and Dichotomomyces based on sequence data. Species in
Neosartorya and Neocarpenteles have anamorphs with green
conidia and share the production of tryptoquivalins, while
Dichotomomyces was found to be able to produce gliotoxin, which is
also produced by some Neosartorya species, and tryptoquivalines and
tryptoquivalones produced by members of both section Clavati and
Fumigati. All species in section Clavati are alkalitolerant
and acidotolerant and they all have clavate conidial heads. Many species are
coprophilic and produce the effective antibiotic patulin. Members of section
Clavati also produce antafumicin, tryptoquivalines, cytochalasins,
sarcins, dehydrocarolic acid and kotanins (orlandin, desmethylkotanin and
kotanin) in species specific combinations. Another species previously assigned
to section Clavati, A. ingratus is considered a synonym of
Hemicarpenteles paradoxus, which is phylogenetically very distantly
related to Neocarpenteles and section Clavati
Polyphasic taxonomy of Aspergillus section Cervini
Species belonging to Aspergillus section Cervini are characterised by radiate or short columnar, fawn coloured, uniseriate conidial heads. The morphology of the taxa in this section is very similar and isolates assigned to these species are frequently misidentified. In this study, a polyphasic approach was applied using morphological characters, extrolite data, temperature profiles and partial BenA, CaM and RPB2 sequences to examine the relationships within this section. Based on this taxonomic approach the section Cervini is resolved in ten species including six new species: A. acidohumus, A. christenseniae, A. novoguineensis, A. subnutans, A. transcarpathicus and A. wisconsinensis. A dichotomous key for the identification is provided
Two novel <i>Aspergillus </i>species from hypersaline soils of The National Park of Lake Urmia, Iran
Two novel Aspergillus species, one belonging to the section Terrei and the other to section Flavipedes, were isolated from hypersaline soils of The National Park of Lake Urmia (Iran) and are here described as Aspergillus iranicus and Aspergillus urmiensis. A polyphasic taxonomic approach comprising extrolite profiles, phenotypic characters and molecular data (beta-tubulin, calmodulin and ribosomal polymerase II second largest subunit gene sequences) was applied to determine their novel taxonomic status. Aspergillus iranicus (CBS 139561T) is phylogenetically related to A. carneus, A. niveus, A. allahabadii and A. neoindicus, and it can be differentiated from those species by a unique extrolite pattern (citrinin, gregatins, and a terrequinone) and its conidial colour. Aspergillus urmiensis (CBS 139558T) shares a most recent common ancestor with A. templicola. The former species produces globose vesicles, and those of A. templicola are predominantly elongate. The Aspergillus urmiensis isolates produce several uncharacterized extrolites. Two other strains obtained during this study reside in a clade, together with the type strain of A. movilensis (CCF 4410T), and are identified accordingly. Based on the phylogenetic data presented in this study, A. frequens is reduced to synonymy with A. micronesiensis and A. mangaliensis is considered to be a synonym of A. templicola
Fleming's penicillin producing streain is not Penicillium chrysogenum but P. rubens
Penicillium chrysogenum is a commonly occurring mould in indoor environments and foods, and has gained much attention for its use in the production of the antibiotic penicillin. Phylogenetic analysis of the most important penicillin producing P. chrysogenum isolates revealed the presence of two highly supported clades, and we show here that these two clades represent two species, P. chrysogenum and P. rubens. These species are phenotypically similar, but extrolite analysis shows that P. chrysogenum produces secalonic acid D and F and/or a metabolite related to lumpidin, while P. rubens does not produce these metabolites. Fleming’s original penicillin producing strain and the full genome sequenced strain of P. chrysogenum are re-identified as P. rubens. Furthermore, the well-known claim that Alexander Fleming misidentified the original penicillin producing strain as P. rubrum is discussed
Mycotoxins and Other Secondary Metabolites Produced in vitro by Penicillium paneum Frisvad and Penicillium roqueforti Thom Isolated from Baled Grass Silage in Ireland
Secondary metabolite profiling, growth profiles and other tools for species recognition and important Aspergillus mycotoxins
Species in the genus Aspergillus have been classified primarily
based on morphological features. Sequencing of house-hold genes has also been
used in Aspergillus taxonomy and phylogeny, while extrolites and
physiological features have been used less frequently. Three independent ways
of classifying and identifying aspergilli appear to be applicable: Morphology
combined with physiology and nutritional features, secondary metabolite
profiling and DNA sequencing. These three ways of identifying
Aspergillus species often point to the same species. This consensus
approach can be used initially, but if consensus is achieved it is recommended
to combine at least two of these independent ways of characterising aspergilli
in a polyphasic taxonomy. The chemical combination of secondary metabolites
and DNA sequence features has not been explored in taxonomy yet, however.
Examples of these different taxonomic approaches will be given for
Aspergillus section Nigri
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