Environmental Law as an Academic Subject

Environmental Law as an Academic Subjec

Jet shoes

Jet shoes for space locomotio

Differential DNA accessibility to polymerase enables 30-minute phenotypic β-lactam antibiotic susceptibility testing of carbapenem-resistant Enterobacteriaceae

The rise in carbapenem-resistant Enterobacteriaceae (CRE) infections has created a global health emergency, underlining the critical need to develop faster diagnostics to treat swiftly and correctly. Although rapid pathogen-identification (ID) tests are being developed, gold-standard antibiotic susceptibility testing (AST) remains unacceptably slow (1–2 d), and innovative approaches for rapid phenotypic ASTs for CREs are urgently needed. Motivated by this need, in this manuscript we tested the hypothesis that upon treatment with β-lactam antibiotics, susceptible Enterobacteriaceae isolates would become sufficiently permeabilized, making some of their DNA accessible to added polymerase and primers. Further, we hypothesized that this accessible DNA would be detectable directly by isothermal amplification methods that do not fully lyse bacterial cells. We build on these results to develop the polymerase-accessibility AST (pol-aAST), a new phenotypic approach for β-lactams, the major antibiotic class for gram-negative infections. We test isolates of the 3 causative pathogens of CRE infections using ceftriaxone (CRO), ertapenem (ETP), and meropenem (MEM) and demonstrate agreement with gold-standard AST. Importantly, pol-aAST correctly categorized resistant isolates that are undetectable by current genotypic methods (negative for β-lactamase genes or lacking predictive genotypes). We also test contrived and clinical urine samples. We show that the pol-aAST can be performed in 30 min sample-to-answer using contrived urine samples and has the potential to be performed directly on clinical urine specimens

Nanosecond electric pulses penetrate the nucleus and enhance speckle formation

Nanosecond electric pulses generate nanopores in the interior membranes of cells and modulate cellular functions. Here, we used confocal microscopy and flow cytometry to observe Smith antigen antibody (Y12) binding to nuclear speckles, known as small nuclear ribonucleoprotein particles (snRNPs) or intrachromatin granule clusters (IGCs), in Jurkat cells following one or five 10 ns, 150 kV/cm pulses. Using confocal microscopy and flow cytometry, we observed changes in nuclear speckle labeling that suggested a disruption of pre-messenger RNA splicing mechanisms. Pulse exposure increased the nuclear speckled substructures by 2.5-fold above basal levels while the propidium iodide (PI) uptake in pulsed cells was unchanged. The resulting nuclear speckle changes were also cell cycle dependent. These findings suggest that 10 ns pulses directly influenced nuclear processes, such as the changes in the nuclear RNA–protein complexes

The Ursinus Weekly, October 7, 1963

Customs program: Class of 1967 well underway • Sororities begin Fall rushing Wed. • Harpsichordist Temple Painter to be featured in first Forum Wednesday • Dr. Foster on sabbatical • Choral groups begin rehearsals • Y retreat set for Oct. 11 & 12 • Sue Harmon in Mr. Roberts • More than 980 enrolled this Fall • Honors rating to 23 freshmen • UC student named president of SNEA • Students ready to teach; Banquet set for tonight • Editorial: Word of welcome • Danforth graduate fellowships available • Dateline: Stockholm, Sweden • Letters to the editor • Three faculty members receive distinguished teaching award • Record enrollment in Evening School • National Security Agency test • From behind my dink • Oral cultural vaccine given • Class \u2763 ranks top in education • Summer research, study for faculty • Susquehanna mauls Bears in opener, 32-0 • Ursinus host to foreign teams • Williamson named most valuable in MAC Southern div. • Vernon Morgan ends track careerhttps://digitalcommons.ursinus.edu/weekly/1252/thumbnail.jp

Collapse of Amphibian Communities Due to an Introduced Ranavirus

This work was supported by Natural Environment Research Council grant NE/G011885/1, the Systematics and Taxonomy (SynTax) research scheme administered by the Linnean Society of London, the Fundación General CSIC, Banco Santander, and European Research Council grant 260801-BIG-IDE

Mitigating amphibian chytridiomycoses in nature

Amphibians across the planet face the threat of population decline and extirpation caused by the disease chytridiomycosis. Despite consensus that the fungal pathogens responsible for the disease are conservation issues, strategies to mitigate their impacts in the natural world are, at best, nascent. Reducing risk associated with the movement of amphibians, non-amphibian vectors and other sources of infection remains the first line of defence and a primary objective when mitigating the threat of disease in wildlife. Amphibian-associated chytridiomycete fungi and chytridiomycosis are already widespread, though, and we therefore focus on discussing options for mitigating the threats once disease emergence has occurred in wild amphibian populations. All strategies have shortcomings that need to be overcome before implementation, including stronger efforts towards understanding and addressing ethical and legal considerations. Even if these issues can be dealt with, all currently available approaches, or those under discussion, are unlikely to yield the desired conservation outcome of disease mitigation. The decision process for establishing mitigation strategies requires integrated thinking that assesses disease mitigation options critically and embeds them within more comprehensive strategies for the conservation of amphibian populations, communities and ecosystems