Recherches biostratigraphiques dans quelques coupes du Famennien de l'Avesnois (Nord de la France)

Conodonts and Goniatites from four "old" famennian sections in the Avesnois (France) have been carefully studies. For the first time, the biostratigraphic position of these sections is determined

Sedimentary environments, conodont biofacies and paleoecology of the belgian Famennian (upper devonian) - an approach

The vertical succession of the sedimentary formations, which are diachronic with respect to the standard conodont and other microfossils zonation, represents a regressive megasequence in the Belgian Famennian, ranging from relatively deep, offshore open marine, shallow nearshore, to backshore restricted marine environments (THOREZ et al., 1977). Different associations of platform conodont form genera (Biofacies) reflect different ecological conditions, which are directly or indirectly related to water depth, turbulency, salinity and proximity to the coast ; from offshore to backshore we recognized successively a Palmatolepid, a Palmatolepid-Polygnathid, a Polygnathid-Icriodid, an Icriodid, and a supposed Clydagnathid biofacies. In this way the distribution pattern of conodont biofacies throughout the Famennian, represents a normal response to the oscillating movements of a prograding "coast" in the studied sedimentary basins. The presence moreover of mixed conodont biofacies (or thanatocoenoses) is also related to sedimentological processes, such as storm wave action, turbidity currents and tidal inlets

Fammenian

The name of the Famennian stage originated in the 19th century from the Famenne region in southern Belgium. After the pioneering work of Dumont, d'Omalius d'Halloy, Gosselet and Mourlon, a renewed interest in the 1960’s focused mainly on the lithological, sedimentological, paleontological and palynological characteristics of the Famennian. This resulted in a refined litho- and biostratigraphical framework for the Lower Famennian Famenne Shales and the Upper Famennian Condroz Sandstones in Belgium. The most obvious finding was the distinct diachronous character of the Condroz Group. The present review paper extends this stratigraphical framework into the neighbouring Avesnois (Northern France) and Aachen (Northwestern Germany) areas: it proposes a lithostratigraphical correlation between the units identified within the individual tectonic units. An overview is given of the main lithological-sedimentological characteristics, depositional environments (for both the siliciclastics and the carbonates) and prevailing paleogeographical-paloeclimatological conditions, during the Famennian stage in Belgium and adjacent areas

Inhibition of DNA damage response at telomeres improves the detrimental phenotypes of Hutchinson–Gilford Progeria Syndrome

Hutchinson–Gilford progeria syndrome (HGPS) is a genetic disorder characterized by premature aging features. Cells from HGPS patients express progerin, a truncated form of Lamin A, which perturbs cellular homeostasis leading to nuclear shape alterations, genome instability, heterochromatin loss, telomere dysfunction and premature entry into cellular senescence. Recently, we reported that telomere dysfunction induces the transcription of telomeric non-coding RNAs (tncRNAs) which control the DNA damage response (DDR) at dysfunctional telomeres. Here we show that progerin-induced telomere dysfunction induces the transcription of tncRNAs. Their functional inhibition by sequence-specific telomeric antisense oligonucleotides (tASOs) prevents full DDR activation and premature cellular senescence in various HGPS cell systems, including HGPS patient fibroblasts. We also show in vivo that tASO treatment significantly enhances skin homeostasis and lifespan in a transgenic HGPS mouse model. In summary, our results demonstrate an important role for telomeric DDR activation in HGPS progeroid detrimental phenotypes in vitro and in vivo

Obtaining material identification with cosmic ray radiography

The passage of muons through matter is mostly affected by their Coulomb interactions with electrons and nuclei. The muon interactions with electrons lead to continuous energy loss and stopping of muons, while their scattering off nuclei lead to angular 'diffusion'. By measuring both the number of stopped muons and angular changes in muon trajectories we can estimate density and identify materials. Here we demonstrate the material identification using data taken at Los Alamos with the Mini Muon Tracker.Comment: 10 pages, 9 figures, Accepted to AIP Advance

Biological Insights into the Expression of Translation Initiation Factors from Recombinant CHOK1SV Cell Lines and their Relationship to Enhanced Productivity

Translation initiation is on the critical pathway for the production of monoclonal antibodies (mAb) by mammalian cells. Formation of a closed loop structure comprised of mRNA, a number of eukaryotic initiation factors and ribosomal proteins has been proposed to aid re-initiation of translation and therefore increase global translational efficiency. We have determined mRNA and protein levels of the key components of the closed loop; eukaryotic initiation factors (eIF3a, eIF3b, eIF3c, eIF3h, eIF3i and eIF4G1), poly(A) binding protein (PABP) 1 and PABP interacting protein 1 (PAIP1) across a panel of 30 recombinant mAb-producing GS-CHOK1SV cell lines with a broad range of growth characteristics and production levels of a model recombinant mAb. We have used a multi-level statistical approach to investigate the relationship between key performance indicators (cell growth and recombinant antibody productivity) and the intracellular amounts of target translation initiation factor proteins and the mRNAs encoding them. We show that high-producing cell lines maintain amounts of the translation initiation factors involved in the formation of the closed loop mRNA, maintaining these proteins at appropriate levels to deliver enhanced recombinant protein production. We then utilise knowledge of the amounts of these factors to build predictive models for, and use cluster analysis to identify, high-producing cell lines. This study therefore defines the translation initiation factor amounts that are associated with highly productive recombinant GS-CHOK1SV cell lines that may be targets for screening highly productive cell lines or to engineer new host cell lines with the potential for enhanced recombinant antibody productivity

Synergy of extreme drought and shrub invasion reduce ecosystem functioning and resilience in water-limited climates

Extreme drought events and plant invasions are major drivers of global change that can critically affect ecosystem functioning and alter ecosystem-atmosphere exchange. Invaders are expanding worldwide and extreme drought events are projected to increase in frequency and intensity. However, very little is known on how these drivers may interact to affect the functioning and resilience of ecosystems to extreme events. Using a manipulative shrub removal experiment and the co-occurrence of an extreme drought event (2011/2012) in a Mediterranean woodland, we show that native shrub invasion and extreme drought synergistically reduced ecosystem transpiration and the resilience of key-stone oak tree species. Ecosystem transpiration was dominated by the water use of the invasive shrub Cistus ladanifer, which further increased after the extreme drought event. Meanwhile, the transpiration of key-stone tree species decreased, indicating a competitive advantage in favour of the invader. Our results suggest that in Mediterranean-type climates the invasion of water spending species and projected recurrent extreme drought events may synergistically cause critical drought tolerance thresholds of key-stone tree species to be surpassed, corroborating observed higher tree mortality in the invaded ecosystems. Ultimately, this may shift seasonally water limited ecosystems into less desirable alternative states dominated by water spending invasive shrubs