Using Community Based Participatory Action Research as Service-learning for Tribal College Students

This work reports the methodological approach used in a Community Based Participatory Research (CBPR) project that incorporated Northern Plains tribal college students from four different communities as data gatherers and co-researchers in their communities. We report preliminary findings of perceptions of service learning among the participating tribal college students based on reflective interviews

Operationalising factors that explain the emergence of infectious diseases : A case study of the human campylobacteriosis epidemic

Peer reviewedPublisher PD

Unicorns and agency theory: Agreeable moral hazard?

The number of unicorns, startups valued over $1 billion, has steadily risen over the past decade. The abnormally high valuation of a unicorn from investors is based on their potential to disrupt a market and create a new paradigm. With this as the backdrop, this piece asks the question, what theoretical tools do we have to understand unicorns? Specifically, we explore agency theory. We argue that if principals and agents agree on the goal of disruption, then perhaps the agency problem that does occur in unicorns is beneficial, not a cost. Further, we argue that the principals of unicorns do want agents to take higher than normal risks with their investment to disrupt a given market. From this phenomenon, we introduce the concept of agreeable moral hazard and its use in the unicorn setting. Not only does the concept of agreeable moral hazard provide theoretical implications for future research, but it also highlights the need for more research to test existing theory on the unicorn population

The Histamine H4 Receptor Mediates Inflammation and Pruritus in Th2-Dependent Dermal Inflammation

The role of histamine H4 receptor (H4R) was investigated in a T-helper type 2 (Th2)-cell-mediated mouse skin inflammation model that mimics several of the features of atopic dermatitis. Treatment with two specific H4R antagonists before challenge with FITC led to a significant reduction in ear edema, inflammation, mast cell, and eosinophil infiltration. This was accompanied by a reduction in the levels of several cytokines and chemokines in the ear tissue. Upon ex vivo antigen stimulation of lymph nodes, H4R antagonism reduced lymphocyte proliferation and IL-4, IL-5, and IL-17 levels. One explanation for this finding is that lymph nodes from animals dosed with the H4R antagonist, JNJ 7777120, contained a lower number of FITC-positive dendritic cells. The effect of H4R antagonism on dendritic cell migration in vivo may be an indirect result of the reduction in tissue cytokines and chemokines or a direct effect on chemotaxis. In addition to anti-inflammatory effects, JNJ 7777120 also significantly inhibited the pruritus shown in the model. Therefore, the dual effects of H4R antagonists on pruritus and Th2-cell-mediated inflammation point to their therapeutic potential for the treatment of Th2-mediated skin disorders, including atopic dermatitis

Development of Low-Cost Micromanipulation Systems for Small Extraterrestrial Samples

The analysis of microscale to mm-scale astromaterials often involves the transfer of samples from storage or collection substrates to analytical substrates. These transfers are accomplished by hand (via tweezers or fine-tipped needles) or by utilizing micromanipulation instruments. Freehand manipulation of small particles is extremely challenging due to involuntary hand tremors on the order of 100m and due to the triboelectric charging induced by frequent contact between the manipulation tool and the support substrate. Months or years of practice may be required before an investigator develops the necessary experience to confidently transfer a 10-20m particle in this manner. Handling even mm-sized particles with fine-tipped tweezers can be challenging, due to the inability to precisely control the force with which grains are being held. Mechanical, hydraulic, and motorized/electrical micromanipulators enable the precise handling of microscale samples and are often utilized in laboratories where frequent small sample preparation is required. However, the price of such instruments (~ $10,000 to$100,000) makes them cost-prohibitive for some institutions. Graduate students or early-career scientists interested in conducting research on interplanetary dust particles, Itokawa particles returned by Hayabusa, or future samples returned by OSIRIS-REx or Hayabusa2 may experience difficulty in justifying the expense of a micromanipulator to their advisors or principle investigators. Johnson Space Centers Astromaterials Acquisition and Curation Office and the Lunar and Planetary Institute conduct annual training for early career scientists and for investigators that require experience with handling of small extraterrestrial samples. In support of this training, we have been developing low-cost mechanical alternatives to expensive micromanipulators that training participants can implement in their respective facilities

Do Cues Matter? Highly Inductive Settlement Cues Don't Ensure High Post-Settlement Survival in Sea Urchin Aquaculture

Increasing settlement and post-settlement survival during the critical transition from planktonic larvae to benthic juveniles will increase efficiency for sea urchin aquaculture. This study investigated the effects of temperature and settlement cues on the settlement and post-settlement survival of the sea urchin Tripneustes gratilla during this phase. The current commercial methodology, which utilises natural biofilm settlement plates, was tested and resulted in low settlement (<2%) and poor post-settlement survival (<1% of settled urchins). In laboratory trials, settlement was high and unaffected by temperatures between 24 and 30°C, but significantly decreased at 33°C. Development of spines, however, was significantly affected by temperatures over 29°C. Mirroring this result, post-settlement survival was optimal between 24–28°C. In laboratory assays, the macroalgae Sargassum linearifolium and Corallina officinalis, and seawater conditioned with these algae, induced significantly higher settlement (>90%) than a natural biofilm (∼25%). The addition of macroalgae-conditioned seawater to natural biofilm significantly increased settlement rates (>85%). Mixed consortia and single strains of bacteria isolated from macroalgae, biofilms and adult conspecifics all induced significant settlement, but at significantly lower rates than macroalgae. No evidence was found that higher rates of settlement to bacteria on macroalgae were generated by a cofactor from the macroalgae. Age of bacterial cultures, culturing bacteria on solid and liquid media and concentration of nutrients in cultures had little effect on settlement rates. Finally, macroalgae-conditioned seawater combined with natural biofilm settlement plates induced significantly higher settlement than to the biofilm plates alone in a commercial scale trial. However, high post-settlement mortality resulted in equivalent survival between treatments after 25 days. This study highlights that settlement studies should extend to post-settlement survival, which remains poor for T. gratilla and is a significant obstacle to increasing efficiency for aquaculture

Investigating Astromaterials Curation Applications for Dexterous Robotic Arms

The Astromaterials Acquisition and Curation office at NASA Johnson Space Center is currently investigating tools and methods that will enable the curation of future astromaterials collections. Size and temperature constraints for astromaterials to be collected by current and future proposed missions will require the development of new robotic sample and tool handling capabilities. NASA Curation has investigated the application of robot arms in the past, and robotic 3-axis micromanipulators are currently in use for small particle curation in the Stardust and Cosmic Dust laboratories. While 3-axis micromanipulators have been extremely successful for activities involving the transfer of isolated particles in the 5-20 micron range (e.g. from microscope slide to epoxy bullet tip, beryllium SEM disk), their limited ranges of motion and lack of yaw, pitch, and roll degrees of freedom restrict their utility in other applications. For instance, curators removing particles from cosmic dust collectors by hand often employ scooping and rotating motions to successfully free trapped particles from the silicone oil coatings. Similar scooping and rotating motions are also employed when isolating a specific particle of interest from an aliquot of crushed meteorite. While cosmic dust curators have been remarkably successful with these kinds of particle manipulations using handheld tools, operator fatigue limits the number of particles that can be removed during a given extraction session. The challenges for curation of small particles will be exacerbated by mission requirements that samples be processed in N2 sample cabinets (i.e. gloveboxes). We have been investigating the use of compact robot arms to facilitate sample handling within gloveboxes. Six-axis robot arms potentially have applications beyond small particle manipulation. For instance, future sample return missions may involve biologically sensitive astromaterials that can be easily compromised by physical interaction with a curator; other potential future returned samples may require cryogenic curation. Robot arms may be combined with high resolution cameras within a sample cabinet and controlled remotely by curator. Sophisticated robot arm and hand combination systems can be programmed to mimic the movements of a curator wearing a data glove; successful implementation of such a system may ultimately allow a curator to virtually operate in a nitrogen, cryogenic, or biologically sensitive environment with dexterity comparable to that of a curator physically handling samples in a glove box

Associations of home confinement during COVID-19 lockdown with subsequent health and well-being among UK adults

During the COVID-19 pandemic, the United Kingdom (UK) government introduced public health safety measures to mitigate the spikes in infection rates. This included stay-at-home orders that prevented people from leaving their homes for work or study, except for urgent medical care or buying essential items. This practice could have both short and long-term implications for health and wellbeing of people in the UK. Using longitudinal data of 10,630 UK adults, this study prospectively examined the association between home confinement status during the stringent lockdown in the UK (March 23-May 13, 2020) and 20 indicators of subjective well-being, social well-being, pro-social/altruistic behaviors, psychological distress, and health behaviors assessed approximately one month after the stringent lockdown ended. All analyses adjusted for socio-demographic characteristics and social isolation status in the beginning of the pandemic. Home confinement during the lockdown was associated with greater subsequent compliance with COVID-19 rules, more perceived major stressors, and a lower prevalence of physical activity. There was modest evidence of associations with lower life satisfaction, greater loneliness, greater depressive symptoms, greater anxiety symptoms, and more perceived minor stressors post-lockdown. However, there was little evidence that home confinement was associated with other indices of subsequent health and well-being. While our study shows that home confinement impacts some indices of subsequent health and wellbeing outcomes even after lockdown, the degree of the psychological adaptation to the difficult confinement behavior remains unclear and should be further studied