Réduction et contrôle du foisonnement filamenteux des boues activées par application d'un régime turbulent

Le foisonnement filamenteux des boues activées est un problème important dans les stations d'épuration du type Boues Activées, car il peut entraîner des pertes de biomasse avec l'eau épurée. Les techniques de lutte utilisées actuellement sont, soit des techniques dites « biologiques » (sélecteurs), soit des techniques chimiques (floculants, biocides). L'objectif de notre travail a été la mise au point d'un procédé original basé sur la création d'une zone de turbulence pour casser les réseaux filamenteux. La réduction et le contrôle du bulking sont obtenus par passage (de durée limitée T1) de la biomasse dans un système venturi, équipée d'une prise d'air. L'efficacité du procédé dépend principalement de la turbulence due à l'auto-aspiration d'air au niveau du rétrécissement du venturi. Cette turbulence contribue d'une part au mécanisme de « cisaillement » des filaments, et d'autre part à la qualité de l'effluent sortant. Une gestion adaptée (aération séquentielle en procédé à faible charge) permet d'obtenir, outre une amélioration de la décantation, une amélioration de l'élimination de l'azote. De plus, le contrôle de la biomasse optimise l'élimination de la pollution carbonée.The loss of biomass in a waste water plant as a result of sludge bulking is an important problem. Biological (ex.: selector configuration) or chemical (ex. : floculating reagents, biocides...) methods habe been used in order to control bulking. A new process based on the generation of turbulence to break the filaments is described. To reduce and control sludge bulking, the biomass is pumped through a venturi equipped with an air inlet. Figure 1 represents the pilot scheme. Two processes can be used : the emulsor with air is introduced on recycling of sludge or is placed in an independent loop. Here, it is the latter that has been used.We tried to reduce bulking by mechanical treatment. The hydrodynamic efficiency of the process depends mostly on the degree of turbulence generated by the waters speed and by the suction of air in the constricted part of the venturi.During the first trials on the plant, we defined the maximum turbulence allowed in order not to break the floc. We could not use a big aeration to prevent defloculation.The main factors to reduce bulking are :- the sequences of usage of the emulsor venturi (the periodicity of the treatment is T),- the speed of the water in the nozzle of the venturi (we can refer to the Reynolds Number Re = Ud/ʋ; but this number can’t really represent the turbulence created),- the duration of the emulsor's application. The emulsor venturi is used during a period T1 with a periodicity T (T>T1), with T1 : n X (VR/qv) where VR/qv is the contact time in the venturi and n is the frequence of recycling.During the period T, it is possible to use continuous aeration or to work with sequential aeration.a) continuous aeration|_________________________| TTime between two applications of the venturi|_______________| T1Venturi durationThe process is the same as the aeration of activated sludge. COD is reduced but only with low load, significant nitrification occurs. Figure 5 shows the efficiency of the continuous aeration process especially versus the sludge index parameter.b) sequential aerationAs we try to remove nitrogen, the sequential aeration is more useful than continuous aeration. A cycle is divided in two periods (T2, T3 with T = T2 + T3), and of course, the duration of the venturi use equal to T1.|_________________________| TTime between two applications of the venturi|_____________| T1Venturi duration|_________________| T2Aeration duration          |_____| T3Anoxic periodFigures 6 and 7 show the efficiency of the emulsor if we consider lB (sludge index) as the main parameter for the sequential operation (T2, T3) and it's also possible to obtain a good removal of nitrogen pollution.Our operations parameters were for these experiments :T = 8 or 12 hoursT1 between 20 min. and 1 hourT2 between 4 and 5 hoursThis process is now patiented and the first industrial applications are now defined

Anthropological and socioeconomic factors contributing to global antimicrobial resistance: a univariate and multivariable analysis

Background Understanding of the factors driving global antimicrobial resistance is limited. We analysed antimicrobial resistance and antibiotic consumption worldwide versus many potential contributing factors. Methods Using three sources of data (ResistanceMap, the WHO 2014 report on antimicrobial resistance, and contemporary publications), we created two global indices of antimicrobial resistance for 103 countries using data from 2008 to 2014: Escherichia coli resistance—the global average prevalence of E coli bacteria that were resistant to third-generation cephalosporins and fluoroquinolones, and aggregate resistance—the combined average prevalence of E coli and Klebsiella spp resistant to third-generation cephalosporins, fluoroquinolones, and carbapenems, and meticillin-resistant Staphylococcus aureus. Antibiotic consumption data were obtained from the IQVIA MIDAS database. The World Bank DataBank was used to obtain data for governance, education, gross domestic product (GDP) per capita, health-care spending, and community infrastructure (eg, sanitation). A corruption index was derived using data from Transparency International. We examined associations between antimicrobial resistance and potential contributing factors using simple correlation for a univariate analysis and a logistic regression model for a multivariable analysis. Findings In the univariate analysis, GDP per capita, education, infrastructure, public health-care spending, and antibiotic consumption were all inversely correlated with the two antimicrobial resistance indices, whereas higher temperatures, poorer governance, and the ratio of private to public health expenditure were positively correlated. In the multivariable regression analysis (confined to the 73 countries for which antibiotic consumption data were available) considering the effect of changes in indices on E coli resistance (R2 0·54) and aggregate resistance (R2 0·75), better infrastructure (p=0·014 and p=0·0052) and better governance (p=0·025 and p<0·0001) were associated with lower antimicrobial resistance indices. Antibiotic consumption was not significantly associated with either antimicrobial resistance index in the multivariable analysis (p=0·64 and p=0·070). Interpretation Reduction of antibiotic consumption will not be sufficient to control antimicrobial resistance because contagion—the spread of resistant strains and resistance genes—seems to be the dominant contributing factor. Improving sanitation, increasing access to clean water, and ensuring good governance, as well as increasing public health-care expenditure and better regulating the private health sector are all necessary to reduce global antimicrobial resistance

Fermi-surface transformation across the pseudogap critical point of the cuprate superconductor La1.6−x_{1.6-x}Nd0.4_{0.4}Srx_{x}CuO4_4

The electrical resistivity $\rho$ and Hall coefficient R$_H$ of the tetragonal single-layer cuprate Nd-LSCO were measured in magnetic fields up to $H = 37.5$ T, large enough to access the normal state at $T \to 0$, for closely spaced dopings $p$ across the pseudogap critical point at $p^\star = 0.235$. Below $p^\star$, both coefficients exhibit an upturn at low temperature, which gets more pronounced with decreasing $p$. Taken together, these upturns show that the normal-state carrier density $n$ at $T = 0$ drops upon entering the pseudogap phase. Quantitatively, it goes from $n = 1 + p$ at $p = 0.24$ to $n = p$ at $p = 0.20$. By contrast, the mobility does not change appreciably, as revealed by the magneto-resistance. The transition has a width in doping and some internal structure, whereby R$_H$ responds more slowly than $\rho$ to the opening of the pseudogap. We attribute this difference to a Fermi surface that supports both hole-like and electron-like carriers in the interval $0.2 < p < p^\star$, with compensating contributions to R$_H$. Our data are in excellent agreement with recent high-field data on YBCO and LSCO. The quantitative consistency across three different cuprates shows that a drop in carrier density from $1 + p$ to $p$ is a universal signature of the pseudogap transition at $T=0$. We discuss the implication of these findings for the nature of the pseudogap phase.Comment: 11 pages, 12 figure

The Routine Use of Antibiotics to Promote Animal Growth Does Little to Benefit Protein Undernutrition in the Developing World

Some persons argue that the routine addition of antibiotics to animal feed will help alleviate protein undernutrition in developing countries by increasing meat production. In contrast, we estimate that, if all routine antibiotic use in animal feed were ceased, there would be negligible effects in these countries. Poultry and pork production are unlikely to decrease by more than 2%. Average daily protein supply would decrease by no more than 0.1 g per person (or 0.2% of total protein intake). Eliminating the routine use of in-feed antibiotics will improve human and animal health, by reducing the development and spread of antibiotic-resistant bacteri

Protection against Clostridium difficile infection in a hamster model by oral vaccination using flagellin FliC-loaded pectin beads

International audienceClostridium difficile flagellin FliC is a highly immunogenic pathogen-associated molecular pattern playing a key role in C. difficile pathogenesis and gut colonization. Here, we designed an oral vaccine against C. difficile with FliC encapsulated into pectin beads for colonic release. Bead stability and FliC retention was confirmed in vitro using simulated intestinal media (SIM), while bead degradation and FliC release was observed upon incubation in simulated colonic media (SCM). The importance of FliC encapsulation into pectin beads for protection against C. difficile was assessed in a vaccination assay using a lethal ham-ster model of C. difficile infection. Three groups of hamsters orally received either FliC-loaded beads or unloaded beads in gastro-resistant capsule to limit gastric degradation or free FliC. Two other groups were immunized with free FliC, one intra-rectally and the other intra-peritoneally. Hamsters were then challenged with a lethal dose of C. difficile VPI 10463. Fifty percent of hamsters orally immunized with FliC-loaded beads survived whereas all hamsters orally immunized with free FliC died within 7 days post challenge. No significant protection was observed in the other groups. Only intra-peritoneally immunized hamsters presented anti-FliC IgG antibodies in sera after immunizations. These results suggest that an oral immunization with FliC-loaded beads probably induced a mucosal immune response, therefore providing a protective effect. This study confirms the importance of FliC encapsulation into pectin beads for a protective oral vaccine against C. difficile

World Health Organization Ranking of Antimicrobials According to Their Importance in Human Medicine: A Critical Step for Developing Risk Management Strategies for the Use of Antimicrobials in Food Production Animals

The use of antimicrobials in food animals creates an important source of antimicrobial-resistant bacteria that can spread to humans through the food supply. Improved management of the use of antimicrobials in food animals, particularly reducing the usage of those that are "critically important” for human medicine, is an important step toward preserving the benefits of antimicrobials for people. The World Health Organization has developed and applied criteria to rank antimicrobials according to their relative importance in human medicine. Clinicians, regulatory agencies, policy makers, and other stakeholders can use this ranking when developing risk management strategies for the use of antimicrobials in food production animals. The ranking allows stakeholders to focus risk management efforts on drugs used in food animals that are the most important to human medicine and, thus, need to be addressed most urgently, such as fluoroquinolones, macrolides, and third- and fourth-generation cephalosporin