577 research outputs found
Utilization and turnover of labile dissolved organic matter by bacterial heterotrophs in eastern north Pacific surface waters
Seawater incubation experiments were conducted in June and October 1992 to examine bacterial utilization of labile dissolved organic matter (DOM) in open ocean surface waters of the eastern North Pacific. Natural plankton extract-DOM (PE-DOM) and selected model compounds were added to seawater samples to evaluate bacterial utilization and respiration rates relative to bacterial carbon production rates for the various amendments. PE-DOM always stimulated bacterial production and DOM utilization, and the primary nitrogen source supporting this bacterial production was dissolved organic nitrogen (DON). Utilization of DON during exponential growth was balanced by the production of ammonium for samples amended with PE-DOM. Bacterial growth efficiencies for samples amended with PE-DOM ranged between 3.4 and 8.8 % and generally were slightly higher in June than in October. Of the model compounds tested, net bacterial biomass production was observed only in samples amended with glucose, glucose plus ammonium (glucose+NH4+), and dissolved free amino acids (DFAA). Bacterial growth efficiencies for these amendments were 0.8, 1.9, and and 9.3.%, respectively. Bacterial production at in situ DOM concentrations was observed in June but not in October. Using the bacterial dissolved organic carbon (DOC) utilization rates observed in this study together with other detailed information pertaining to bulk DOC at our study site, we estimate that the turnover time for labile DOC in these surface waters ranges from approximately 2 to 6 d depending on the labiliity of the standing stock of DOC. On the basis of (a) the exclusive use of DON as a nitrogen source in PE-DOM amendments, (b) the stimulation of ammonium utilization in the glucose+NH4+ amendment, and (c) the higher growth efficiencies observed for samples amended with either PE-DOM or DFAA, we suggest that bacterioplankton biomass production in eastern North Pacific surface waters is primarily energy limited. As a result of this energy limitation, bacterial production appears to be additionally constrained by the quality of the nutrients available for assimilation. Thus, the quality of the DOM substrate, specifically the DOC:DON ratio, can be a major determinant of bacterial production in pelagic marine systems
Radiocarbon in marine bacteria: Evidence for the ages of assimilated carbon
It is generally accepted that marine bacteria utilize labile, recently produced components of bulk dissolved organic matter. This interpretation is based largely on indirect measurements using model compounds and plankton-derived organic matter. Here, we present an assessment of the relative proportions of modem and older dissolved organic carbon (DOC) utilized by marine bacteria. Bacterial nucleic acids were collected from both estuarine (Santa Rosa Sound, FL) and open-ocean (eastern North Pacific) sites, and the natural radiocarbon signatures of the nucleic acid carbon in both systems were determined. Bacterial nucleic acids from Santa Rosa Sound were significantly enriched in radiocarbon with respect to the bulk DOC and were similar to the radiocarbon signature of atmospheric CO2 at the time of sampling, indicating that these bacteria exclusively assimilate a modem component of the estuarine bulk DOG. In contrast, bacterial nucleic acids from the oceanic site were enriched in C-14 relative to the bulk DOC but depleted in C-14 with respect to modem surface dissolved inorganic carbon (DIC) and suspended particulate organic carbon (POCsusp). This suggests that open-ocean bacteria assimilate both modem and older components of DOG. The distinct radiocarbon signatures of the nucleic acids at these two sites (i.e., +120 +/- 17% estuarine vs. -34 +/- 24% oceanic) demonstrate that natural C-14 abundance measurements of bacterial biomarkers are a powerful tool for investigations of carbon cycling through microbial communities in different aquatic systems
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In Situ Hydrocarbon Degradation by Indigenous Nearshore Bacterial Populations
Potential episodic hydrocarbon inputs associated with oil mining and transportation together with chronic introduction of hydrocarbons via urban runoff into the relatively pristine coastal Florida waters poses a significant threat to Florida's fragile marine environment. It is therefore important to understand the extent to which indigenous bacterial populations are able to degrade hydrocarbon compounds and also determine factors that could potentially control and promote the rate at which these compounds are broken down in situ. Previous controlled laboratory experiments carried out by our research group demonstrated that separately both photo-oxidation and cometabolism stimulate bacterial hydrocarbon degradation by natural bacterial assemblages collected from a chronically petroleum contaminated site in Bayboro Bay, Florida. Additionally, we also demonstrated that stable carbon and radiocarbon abundances of respired CO{sub 2} could be used to trace in situ hydrocarbon degradation by indigenous bacterial populations at this same site. This current proposal had two main objectives: (a) to evaluate the cumulative impact of cometabolism and photo-oxidation on hydrocarbon degradation by natural bacterial assemblages collected the same site in Bayboro Bay, Florida and (b) to determine if in situ hydrocarbon degradation by indigenous bacterial populations this site could be traced using natural radiocarbon and stable carbon abundances of assimilated bacterial carbon. Funds were used for 2 years of full support for one ESI Ph.D. student, April Croxton. To address our first objective a series of closed system bacterial incubations were carried out using photo-oxidized petroleum and pinfish (i.e. cometabolite). Bacterial production of CO{sub 2} was used as the indicator of hydrocarbon degradation and {delta}{sup 13}C analysis of the resultant CO{sub 2} was used to evaluate the source of the respired CO{sub 2} (i.e. petroleum hydrocarbons or the pinfish cometabolite). Results from these time series experiments demonstrated that short-term exposure of petroleum to UV light enhanced hydrocarbon degradation by 48% over that observed for non-photo-oxidized petroleum. Despite the greater bio-availability of the photo-oxidized over the non-photo-oxidized petroleum, an initial lag in CO{sub 2} production was observed indicating potential phototoxicity of the photo- by-products. {delta}{sup 13}C analysis and mass balance calculations reveal that co-metabolism with pinfish resulted in increased hydrocarbon degradation for both photo-oxidized and non-photo-oxidized petroleum each by over 100%. These results demonstrate the cumulative effect of photo-oxidation and co-metabolism on petroleum hydrocarbon degradation by natural bacterial populations indigenous to systems chronically impacted by hydrocarbon input. To address the second objective of this proposal bacterial concentrates were collected from Bayboro Harbor in April 2001 for nucleic acid extraction and subsequent natural radiocarbon abundance analyses. Unfortunately, however, all of these samples were lost due to a faulty compressor in our -70 freezer. The freezer was subsequently repaired and samples were again collected from Bayboro Harbor in June 2002 and again December 2002. Several attempts were made to extract the nucleic acid samples--however, the student was not able to successfully extract and an adequate amount of uncontaminated nucleic acid samples for subsequent natural radiocarbon abundance measurements of the bacterial carbon by accelerator mass spectrometry (i.e. require at least 50 {micro}g carbon for AMS measurement). Consequently, we were not able to address the second objective of this proposed work
Assessing sources and ages of organic matter supporting river and estuarine bacterial production: A multiple-isotope (D14C, d13C, and d15N) approach
We used radiocarbon (D14C) and stable isotopic (d13C, d15N) signatures of bacterial nucleic acids to estimate the sources and ages of organic matter (OM) assimilated by bacteria in the Hudson River and York River estuary. Dualisotope plots of D14C and d13C coupled with a three-source mixing model resolved the major OM sources supporting bacterial biomass production (BBP). However, overlap in the stable isotopic (d13C and d15N) values of potential source end members (i.e., terrestrial, freshwater phytoplankton, and marsh-derived) prohibited unequivocal source assignments for certain samples. In freshwater regions of the York, terrigenous material of relatively recent origin (i.e., decadal in age) accounted for the majority of OM assimilated by bacteria (49–83%). Marsh and freshwater planktonic material made up the other major source of OM, with 5–33% and 6–25% assimilated, respectively. In the mesohaline York, BBP was supported primarily by estuarine phytoplankton–derived OM during spring and summer (53–87%) and by marsh-derived OM during fall (as much as 83%). Isotopic signatures from higher salinity regions of the York suggested that BBP there was fueled predominantly by either estuarine phytoplankton-derived OM (July and November) or by material advected in from the Chesapeake Bay proper (October). In contrast to the York, BBP in the Hudson River estuary was subsidized by a greater portion (up to ;25%) of old (;24,000 yr BP) allochthonous OM, which was presumably derived from soils. These findings collectively suggest that bacterial metabolism and degradation in rivers and estuaries may profoundly alter the mean composition and age of OM during transport within these systems and before its export to the coastal ocean
Estimates for the Sobolev trace constant with critical exponent and applications
In this paper we find estimates for the optimal constant in the critical
Sobolev trace inequality S\|u\|^p_{L^{p_*}(\partial\Omega) \hookrightarrow
\|u\|^p_{W^{1,p}(\Omega)} that are independent of . This estimates
generalized those of [3] for general . Here is the
critical exponent for the immersion and is the space dimension. Then we
apply our results first to prove existence of positive solutions to a nonlinear
elliptic problem with a nonlinear boundary condition with critical growth on
the boundary, generalizing the results of [16]. Finally, we study an optimal
design problem with critical exponent.Comment: 22 pages, submitte
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