Creativity: Harmony, Not Noise

A break-press operator boosted the output of crimped, formed machinery shields 10 per cent daily by changing the way he inserted the sheet steel

Residence Hall Program and Perception of University Environment

I have always maintained that at-risk students were often blamed for their lack of success, rather than supported to overcome their prior educational challenges. As a second year residence hall director I had the privilege of working in a building with two distant wings. With the support of my mentor, Dr. Eugene J. Cech, Director of Testing, we developed a residence life program designed to assist summer lower-quarter high school graduates to be successful in college. The quality of residence life does make a difference. The journal, College Student Survey, no longer appears to be published

Mass Marking of Mosquitofish: Preliminary Results

Three marking techniques were tested to determine their applicability for mosquitofish. Tetracycline drugs and DC Ar administered in the diet successfully marked laboratory-cultured mosquitofish, but exposure to direct sunlight in outdoor tanks resulted in the rapid disappearance of the marks. Preliminary data on fluorescent marks from a polystyrene pigment in a melamine-sulfonamide-formaldehyde resin forced into the dermal ,tissue with compressed air are more promising. A t an optimal deliver pressure of 7.3 m Hg (140 p.s.i.) and spraying time of 15 sec., marking percentage is maximized and fish mortality is minimized. Mark retention time was up to 80 days in outdoor tanks at this writing, Long retention times and the availability of four colors increases the potential of this marking technique as a useful tool in field population studies of mosquito fish

Effect of Fish Size on Prey Size Selection in Gambusia Affinis

Food size selection of the mosquitofish , Gambusia affinis affinis. was measured in aquaria using juvenile stages of the mosquito, Clllex tarsalb;, as prey. Fish size varied from recently born fry to large adult females. Food size selection was positively correlated with fish size. Mosquitofish fry (6-8 111m standard length) attacked and ate primarily first and second instar larvae. Fry attacked larger instars, but attack success on these was low (0 - 50%). Fish larger than 20 mm attached primarily pupae and third and fourth instar larva. No first instar mosquitoes were eaten. Attack success for these fish was above 65\u27Yr) for all instars

Efficacy of a sensory deterrent and pipe modifications in decreasing entrainment of juvenile green sturgeon (Acipenser medirostris) at unscreened water diversions.

Water projects designed to extract fresh water for local urban, industrial and agricultural use throughout rivers and estuaries worldwide have contributed to the fragmentation and degradation of suitable habitat for native fishes. The number of water diversions located throughout the Sacramento-San Joaquin watershed in California's Central Valley exceeds 3300, and the majority of these are unscreened. Many anadromous fish species are susceptible to entrainment into these diversions, potentially impacting population numbers. In the laboratory, juvenile green sturgeon (Acipenser medirostris) have been shown to have high entrainment rates into unscreened diversions compared with those of other native California fish species, which may act as a significant source of mortality for this already-threatened species. Therefore, we tested the efficacy of a sensory deterrent (strobe light) and two structural pipe modifications (terminal pipe plate and upturned pipe configuration) in decreasing the entrainment of juvenile green sturgeon (mean mass ± SEM = 162.9 ± 4.0 g; mean fork length = 39.4 ± 0.3 cm) in a large (>500 kl) outdoor flume fitted with a water-diversion pipe 0.46 m in diameter. While the presence of the strobe light did not affect fish entrainment rates, the terminal pipe plate and upturned pipe modifications significantly decreased the proportion of fish entrained out of the total number tested relative to control conditions (0.13 ± 0.02 and 0.03 ± 0.02 vs. 0.44 ± 0.04, respectively). These data suggest that sensory deterrents using visual stimuli are not an effective means to reduce diversion pipe interactions for green sturgeon, but that structural alterations to diversions can successfully reduce entrainment for this species. Our results are informative for the development of effective management strategies to mitigate the impacts of water diversions on sturgeon populations and suggest that effective restoration strategies that balance agricultural needs with conservation programmes are possible

Unscreened water-diversion pipes pose an entrainment risk to the threatened green sturgeon, Acipenser medirostris.

Over 3,300 unscreened agricultural water diversion pipes line the levees and riverbanks of the Sacramento River (California) watershed, where the threatened Southern Distinct Population Segment of green sturgeon, Acipenser medirostris, spawn. The number of sturgeon drawn into (entrained) and killed by these pipes is greatly unknown. We examined avoidance behaviors and entrainment susceptibility of juvenile green sturgeon (35±0.6 cm mean fork length) to entrainment in a large (>500-kl) outdoor flume with a 0.46-m-diameter water-diversion pipe. Fish entrainment was generally high (range: 26-61%), likely due to a lack of avoidance behavior prior to entering inescapable inflow conditions. We estimated that up to 52% of green sturgeon could be entrained after passing within 1.5 m of an active water-diversion pipe three times. These data suggest that green sturgeon are vulnerable to unscreened water-diversion pipes, and that additional research is needed to determine the potential impacts of entrainment mortality on declining sturgeon populations. Data under various hydraulic conditions also suggest that entrainment-related mortality could be decreased by extracting water at lower diversion rates over longer periods of time, balancing agricultural needs with green sturgeon conservation

RNA Recognition by the DNA End-Binding Ku Heterodimer

Most nucleic acid-binding proteins selectively bind either DNA or RNA, but not both nucleic acids. The Saccharomyces cerevisiae Ku heterodimer is unusual in that it has two very different biologically relevant binding modes: (1) Ku is a sequence-nonspecific double-stranded DNA end-binding protein with prominent roles in nonhomologous end-joining and telomeric capping, and (2) Ku associates with a specific stem–loop of TLC1, the RNA subunit of budding yeast telomerase, and is necessary for proper nuclear localization of this ribonucleoprotein enzyme. TLC1 RNA-binding and dsDNA-binding are mutually exclusive, so they may be mediated by the same site on Ku. Although dsDNA binding by Ku is well studied, much less is known about what features of an RNA hairpin enable specific recognition by Ku. To address this question, we localized the Ku-binding site of the TLC1 hairpin with single-nucleotide resolution using phosphorothioate footprinting, used chemical modification to identify an unpredicted motif within the hairpin secondary structure, and carried out mutagenesis of the stem–loop to ascertain the critical elements within the RNA that permit Ku binding. Finally, we provide evidence that the Ku-binding site is present in additional budding yeast telomerase RNAs and discuss the possibility that RNA binding is a conserved function of the Ku heterodimer