Alternative Lengthening of Telomeres Is Characterized by High Rates of Telomeric Exchange

Abstract Telomere maintenance activity is a hallmark of cancer. In some telomerase-negative tumors, telomeres become lengthened by alternative lengthening of telomeres (ALT), a recombination-mediated DNA replication process in which telomeres use other telomeric DNA as a copy template. Using chromosome orientation fluorescence in situ hybridization, we found that postreplicative exchange events involving a telomere and another TTAGGG-repeat tract occur at remarkably high frequencies in ALT cells (range 28–280/100 metaphases) and rarely or never in non-ALT cells, including cell lines with very long telomeres. Like the ALT phenotype itself, the telomeric exchanges were not suppressed when telomerase was activated in ALT cells. These exchanges are telomere specific because there was no correlation with sister chromatid exchange rates at interstitial locations, and they were not observed in non-ALT Bloom syndrome cells with very high sister chromatid exchange rates

Alternative Fourier Expansions for Inverse Square Law Forces

Few-body problems involving Coulomb or gravitational interactions between pairs of particles, whether in classical or quantum physics, are generally handled through a standard multipole expansion of the two-body potentials. We discuss an alternative based on a compact, cylindrical Green's function expansion that should have wide applicability throughout physics. Two-electron "direct" and "exchange" integrals in many-electron quantum systems are evaluated to illustrate the procedure which is more compact than the standard one using Wigner coefficients and Slater integrals.Comment: 10 pages, latex/Revtex4, 1 figure

Aged Drilled Cuttings Offshore Gabon: New Methodology for Assessing Their Impact

During E&P offshore activities, and in particular during drilling operations, the living conditions of the flora and fauna on the seabed may be disturbed.The paper describes the study made for assessing the actual impact of the discharged cuttings, the regenerating capacity of the ecosystem and the medium and long-term recolonization processes, based on in situ measurements. A previous study undertaken on cuttings recently discharged offshore Congo measured kinetics of space and time restoration of the drilling site. To validate the results we carried out a similar study dedicated on aged cuttings offshore Gabon. Usual process for monitoring biological effects of cuttings includes several approaches such as benthic macrofauna (> 1mm size) studies, physicochemical analysis of sediment and ecotoxicological tests. One of the key features of our study was to add, to this process, a study of the benthic meiofauna (0.1 to 1mm size). Experiments conducted demonstrated: -very low concentrations of hydrocarbons stemming out from the cuttings, -a very low toxicity of sediment, -no benthic macrofauna community alteration, -no change in the communities of actual and fossil foraminifera (meiofauna) and, -last but not least, that the study of the benthic meiofauna improves the quality of the assessment made

Tagging and localization of ionizing events using NbSi transition edge phonon sensors for dark matter searches

In the context of direct searches of sub-GeV dark matter particles with germanium detectors, the EDELWEISS collaboration has tested a new technique to tag ionizing events using NbSi transition edge athermal phonon sensors. The emission of the athermal phonons generated by the Neganov-Trofimov-Luke effect associated with the drift of electrons and holes through the detectors is used to tag ionization events generated in specific parts of the detector localized in front of the NbSi sensor and to reject by more than a factor 5 [at 90% confidence level (CL)] the background from heat-only events that dominates the spectrum above 3 keV. This method is able to improve by a factor of 2.8 the previous limit on spinindependent interactions of 1 GeV=c2 weakly interacting massive particles obtained with the same detector and dataset but without this tagging technique.The help of the technical staff of the Laboratoire Souterrain de Modane and the participant laboratories is gratefully acknowledged. The EDELWEISS project is supported in part by the French Agence Nationale pour la Recherche (ANR-21-CE31-0004), by the P2IO LabEx (ANR-10-LABX-0038) in the framework “Investissements d’Avenir” (ANR-11-IDEX-0003-01), and the LabEx Lyon Institute of Origins (ANR-10-LABX-0066) within the framework of the program France 2030, also operated by the National Research Agency of France. B. J. Kavanagh thanks the Spanish Agencia Estatal de Investigación (AEI, MICIU) for the support to the Unidad de Excelencia María de Maeztu Instituto de Física de Cantabria, Ref. MDM-2017-0765. B. J. K. also acknowledges funding from the Ramón y Cajal Grant RYC2021-034757-I, financed by MCIN/AEI/10.13039/ 501100011033 and by the European Union “NextGenerationEU”/PRTR

Non-Linear Evolution of the r-Modes in Neutron Stars

The evolution of a neutron-star r-mode driven unstable by gravitational radiation (GR) is studied here using numerical solutions of the full non-linear fluid equations. The amplitude of the mode grows to order unity before strong shocks develop which quickly damp the mode. In this simulation the star loses about 40% of its initial angular momentum and 50% of its rotational kinetic energy before the mode is damped. The non-linear evolution causes the fluid to develop strong differential rotation which is concentrated near the surface and especially near the poles of the star.Comment: 4 pages, 7 eps figures, revtex; revised, typos correcte

Search for sub-GeV dark matter via the Migdal effect with an EDELWEISS germanium detector with NbSi transition-edge sensors

The EDELWEISS collaboration reports on the search for dark matter particle interactions via Migdal effect with masses between 32 MeV · c−2 to 2 GeV · c−2 using a 200 g cryogenic Ge detector sensitive to simultaneously heat and ionization signals and operated underground at the Laboratoire Souterrain de Modane in France. The phonon signal was read out using a transition edge sensor made of a NbSi thin film. The detector was biased at 66 V in order to benefit from the Neganov-Trofimov-Luke amplification and resulting in a resolution on the energy of electron recoils of 4.46 eVee (102.58 eV at 66 V) and an analysis threshold of 30 eVee. The sensitivity is limited by a dominant background not associated to charge creation in the detector. The search constrains a new region of parameter space for cross sections down to 10−29 cm2 and masses between 32 and 100 MeV · c−2. The achieved low threshold with the NbSi sensor shows the relevance of its use for out-of-equilibrium phonon sensitive devices for low-mass dark matter searches.The EDELWEISS project is supported in part by the French Agence Nationale pour la Recherche (ANR) and the LabEx Lyon Institute of Origins (ANR-10-LABX 0066) of the Universit´e de Lyon within the program “Investissements d’Avenir” (ANR-11-IDEX-00007), by the P2IO LabEx (ANR-10-LABX-0038) in the framework “Investissements d’Avenir” (ANR-11-IDEX-0003-01) managed by the ANR (France), and the Russian Foundation for Basic Research (Grant No. 18-02- 00159). This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Grant Agreement No. 838537. B. J. K. thanks the Spanish Agencia Estatal de Investigación (AEI, MICIU) for the support to the Unidad de Excelencia María de Maeztu Instituto de Física de Cantabria, Ref. MDM-2017-0765. We thank J. P. Lopez (IP2I) and the Physics Department of Universit´e Lyon 1 for their contribution to the radioactive sources

Axion searches with the EDELWEISS-II experiment

We present new constraints on the couplings of axions and more generic axion-like particles using data from the EDELWEISS-II experiment. The EDELWEISS experiment, located at the Underground Laboratory of Modane, primarily aims at the direct detection of WIMPs using germanium bolometers. It is also sensitive to the low-energy electron recoils that would be induced by solar or dark matter axions. Using a total exposure of up to 448 kg.d, we searched for axion-induced electron recoils down to 2.5 keV within four scenarios involving different hypotheses on the origin and couplings of axions. We set a 95% CL limit on the coupling to photons $g_{A\gamma}<2.13\times 10^{-9}$ GeV$^{-1}$ in a mass range not fully covered by axion helioscopes. We also constrain the coupling to electrons, $g_{Ae} < 2.56\times 10^{-11}$, similar to the more indirect solar neutrino bound. Finally we place a limit on $g_{Ae}\times g_{AN}^{\rm eff}<4.70 \times 10^{-17}$, where $g_{AN}^{\rm eff}$ is the effective axion-nucleon coupling for $^{57}$Fe. Combining these results we fully exclude the mass range $0.91\,{\rm eV}<m_A<80$ keV for DFSZ axions and $5.73\,{\rm eV}<m_A<40$ keV for KSVZ axions