大学说明性课程中参与式互动的规范。基于观察方法作为混合方法策略的共建训练建议

El objetivo de este artículo es compartir algunas consideraciones de fondo y procedimiento sobre la comunicación académica modelada por la interacción participativa en el marco de la formación co-constructiva del profesorado. A partir de un estudio de caso múltiple en aula universitaria se analiza el formato expositivo-magistral con actores motivados a sostener su posición dialógica de rol en la interacción comunicativa y facilitar los actos de ayuda recíproca, recursos discursivos e interactivos que cumplen una función estratégica porque son necesarios para la construcción intelectual y autónoma del aprendizaje. La investigación empírica se diseña como un estudio de observación sistemática con un propósito evaluativo y que implementa la integración de los análisis cualitativos y cuantitativos desde un enfoque mixed methods. La discusión de los resultados pone en valor la flexibilidad y la adecuación del método utilizado tanto en el plano de la descripción matizada del proceso de regulación de la interacción participativa intentada por los actores como en el plano de la evaluación formativa de los puntos fuertes y débiles observados.The objective of this article is to share some background and procedural considerations on academic communication modeled by participatory interaction in the co-constructive training framework for teachers. From a case study in a university classroom, the expository-lectures format is analyzed with actors motivated to sustain their dialogical position of role in communicative interaction and facilitate acts of reciprocal help as well as discursive and interactive resources that fulfill a strategic function because they are necessary for the intellectual and autonomous construction of learning. Empirical research is designed as a systematic observation study with an evaluative purpose and that implements the integration of qualitative and quantitative analysis from a «mixed methods» approach. The discussion of the results highlights the flexibility and suitability of the method used both in terms of the nuanced description of the participatory interaction process attempted by the actors and in terms of the formative assessment of the strengths and weaknesses of the expository-lectures observed.Цель данной статьи - поделиться некоторыми исходными данными и процедурными соображениями об академической коммуникации, моделируемой партисипативным взаимодействием в рамках ко-конструктивного обучения преподавателей. На примере университетской аудитории анализируется формат докладов-лекций с действующими лицами, мотивированными на поддержание своей диалогической роли в коммуникативном взаимодействии и содействие актам взаимной помощи, а также дискурсивные и интерактивные ресурсы, выполняющие стратегическую функцию, поскольку они необходимы для интеллектуального и автономного построения обучения. Эмпирическое исследование построено как систематическое наблюдательное исследование с оценочной целью, в котором реализована интеграция качественного и количественного анализа на основе подхода «смешанных методов». Обсуждение результатов подчеркивает гибкость и пригодность используемого метода как в плане детального описания процесса партисипативного взаимодействия, который пытались осуществить участники, так и в плане формативной оценки сильных и слабых сторон наблюдаемых докладов-лекций.本文的目的是在对教师的共建培训框架内分享以参与式互动为模型的学术交流的一些背景及其过程。我们从大学课堂的多个案例研究中分析了说明性-权威模式，其中参与者被鼓励坚持其在交流互动中的对话角色地位，并推动互惠帮助的行为和具有战略功能的话语和互动资源，因为它们是知识与自主学习建构所必需的。该实证研究被设计为具有评估目的的系统观察研究，从混合方法角度整合了定性和定量分析。对结果的讨论中从两方面强调了该方法的灵活性和充分性，一方面为对参与者尝试的参与式互动过程的细致描述方面，另一方面是在对所观察到的说明性讲座的优缺点的形成性评估方面

Movement Notation Revisited: Syntax of the Common Morphokinetic Alphabet (CMA) System

Advances in the study of non-verbal behavior and communication have generated a need for movement transcription systems capable of incorporating continuous developments in visual and computer technology. Our research team has been working on the construction of a common morphokinetic alphabet (CMA) for the systematic observation of daily life activities. The project, which was launched several years ago, was designed to create a system for describing and analyzing body motion expression, physical activity, and physical appearance. In this paper, we describe an idiosyncratic application of Noam Chomsky’s phrase marker grammar to the morphokinetic phrase, the objective being to establish the grammatical rules and basic order of the symbol string according to a relational tree formed by the breakdown of the syntactic components identified as structuring the visual description of movement. Criteria for using the CMA as a coding system and a free transcription system are proposed

In vitro developmental competence of prepubertal goat oocytes cultured with recombinant activin-A

The present study was designed to evaluate the effect of activin-A during the in vitro oocyte maturation (IVM) and in vitro embryo culture (IVC) on nuclear maturation, blastocyst yield and blastocyst quality of prepubertal goat oocytes. In Experiment 1, three groups of oocytes were used during the IVM of prepubertal goat oocytes to determine the optimal concentration of recombinant human activin-A added to the maturation medium. Cumulus-oocyte complexes were matured in an IVM medium containing 0, 10 and 100 ng/ml (groups A0, A10 and A100), fertilized and in vitro cultured using standard procedures. In Experiment 2, the addition of 10 ng/ml activin-A at IVM (A10A0), IVC (A0A10) or IVM+IVC (A10A10) was studied and compared with the control group (A0A0). Results of the first experiment demonstrated that the addition of activin-A yielded similar percentages of maturation (⩽71.0%) and blastocyst formation rates (⩽24.9%) than the control group (A0). Experiment 2 showed that exposure of prepubertal goat oocytes to an IVC medium containing 10 ng/ml activin-A (A0A10) significantly increased the rates of development to the blastocyst stage, as compared with the control group (A0A0) (19.5±2.21% v. 13.1±2.37%, respectively; P<0.05). With regard to the blastocyst quality, total number of cells, inner cell mass (ICM) and trophectoderm of prepubertal goat embryos produced in the presence of activin-A did not differ significantly among experimental groups. In summary, these results indicate that supplementation of the IVC medium with activin-A enhances embryo development of prepubertal goat oocytes

Influence of activation conditions on textural properties and performance of activated biochars for pyrolysis vapors upgrading

The main aim of the present study is to provide a comprehensive assessment of the effects of process activation conditions on the textural properties of the resulting activated carbons, which were produced from wheat straw-derived biochar through chemical activation (with K2CO3 at different pressures and mass impregnation ratios) and physical activation (with CO2 at different temperatures and pressures). For chemically activated biochars, it was found that specific surface area and pore size distribution were both only positively affected by increasing the carbonate loading. However, physically activated biochars produced at the highest pressure and lowest temperature (1.0 MPa and 700 °C) had the highest surface areas and widest pore size distributions. The materials with the most appropriate textural properties were then tested as catalysts for steam and dry reforming of the aqueous phase of pyrolysis oil. The best catalytic performance (a total gas yield of 74% and a selectivity toward H2 of almost 40%) was observed for a physically activated biochar. This good performance was ascribed to the high availability of K0 on the catalyst surface, which could effectively promote the reactions involved in the upgrading proces

Generation of a human iPSC line from a patient with Leigh syndrome

Human iPSC line LND554SV.3 was generated from heteroplasmic fibroblasts of a patient with Leigh syndrome carrying a mutation in the MT-ND5 gene (m.13513G. &gt;. A; p.D393N). Reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using a non-integrative methodology that involves the use of Sendai virus.This work was supported by grants from the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER) (grant 13-717/132.05 to RG), the “Instituto de Salud Carlos III” [Fondo de Investigación Sanitaria and Regional Development Fund (ERDF/FEDER) funds PI10/0703 and PI13/00556 to RG and PI15/00484 to MEG], “Comunidad Autónoma de Madrid” (grant number S2010/BMD-2402 to RG); TG receives grant support from the Universidad Autónoma de Madrid (FPI-UAM) and FZD from the Ministerio de Educación, Cultura y Deporte (FPU13/00544). MEG is a staff scientist at the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER) at the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER)

Generation of a human control iPSC line with a European mitochondrial haplogroup U background

Human iPSC line N44SV.5 was generated from primary normal human dermal fibroblasts belonging to the European mitochondrial haplogroup U. For this purpose, reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using a non-integrative methodology that involves the use of Sendai virus.This work was supported by grants from the “Centro de Investigación Biomédica en Red en enfermedades raras” (CIBERER) (grant 13-717/132.05 to RG), the “Instituto de Salud Carlos III” [Fondo de Investigación Sanitaria and Regional Development Fund (ERDF/FEDER) funds PI10/0703 and PI13/00556 to RG and PI15/00484 to MEG], “Comunidad Autónoma de Madrid” (grant number S2010/BMD-2402 to R.G); T.G. receives grant support from the Universidad Autónoma de Madrid, FPI-UAM and F.Z.D. from the Ministerio de Educación, Cultura y Deporte, grant number FPU13/00544. M.E.G. is staff scientist at the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER

Generation of a human iPSC line from a patient with a mitochondrial encephalopathy due to mutations in the GFM1 gene

Human iPSC line GFM1SV.25 was generated from fibroblasts of a child with a severe mitochondrial encephalopathy associated with mutations in the GFM1 gene, encoding the mitochondrial translation elongation factor G1. Reprogramming factors OCT3/4, SOX2, CMYC and KLF4 were delivered using a non integrative methodology that involves the use of Sendai virus.This work was supported by grants from the “Centro de Investigación Biomédica en Red en enfermedades raras” (CIBERER) (Grant 13-717/132.05 to RG), the “Instituto de Salud Carlos III” [Fondo de Investigación Sanitaria and Regional development fund (ERDF/FEDER) funds PI10/0703 and PI13/00556 to RG and PI15/00484 to MEG], “Comunidad Autónoma de Madrid” (Grant number S2010/BMD-2402 to RG); TG receives grant support from the Universidad Autónoma de Madrid (FPI-UAM) and FZD from the Ministerio de Educación, Cultura y Deporte (Grant FPU13/00544). MEG is staff scientist at the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER

Generation of a human iPSC line from a patient with Leigh syndrome caused by a mutation in the MT-ATP6 gene

Human iPSC line L749.1 was generated from fibroblasts of a patient with Leigh syndrome associated with a heteroplasmic mutation in the MT-ATP6 gene. Reprogramming factors OCT4, SOX2, CMYC and KLF4 were delivered using retroviruses.This work was supported by grants from the “Centro de Investigación Biomédica en Red en enfermedades raras” (CIBERER) (Grant 13-717/132.05 to RG), the “Instituto de Salud Carlos III” (FIS PI10/0703 and PI13/00556 to RG and PI15/00484 to MEG cofunded by FEDER), “Comunidad Autónoma de Madrid” (grant number S2010/BMD-2402 to R.G); T.G-M. receives grant support from the Universidad Autónoma de Madrid, FPI-UAM and F.Z-D. from the Ministerio de Educación, Cultura y Deporte, grant number FPU13/00544. M.E.G. is senior staff scientist at the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER

Generation of a human iPSC line from a patient with a defect of intergenomic communication

Human iPSC line PG64SV.2 was generated from fibroblasts of a patient with a defect of intergenomic communication. This patient harbored a homozygous mutation (c.2243G&gt;C; p.Trp748Ser) in the gene encoding the catalytic subunit of the mitochondrial DNA polymerase gamma gene (POLG). Reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using a non integrative methodology that involves the use of Sendai virus.This work was supported by grants from the “Centro de Investigación Biomédica en Red en enfermedades raras” (CIBERER) (Grant 13-717/132.05 to RG), the “Instituto de Salud Carlos III” [Fondo de Investigación Sanitaria and Regional development fund (ERDF/FEDER) funds PI10/0703 and PI13/00556 to RG and PI15/00484 to MEG], “Comunidad Autónoma de Madrid” (Grant number S2010/BMD-2402 to RG); TG receives grant support from the Universidad Autónoma de Madrid (FPI-UAM) and FZD from the Ministerio de Educación, Cultura y Deporte (Grant FPU13/00544). MEG is staff scientist at the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER

Generation of a human iPSC line from a patient with an optic atrophy ‘plus’ phenotype due to a mutation in the OPA1 gene

AbstractHuman iPSC line Oex2054SV.4 was generated from fibroblasts of a patient with an optic atrophy ‘plus’ phenotype associated with a heterozygous mutation in the OPA1 gene. Reprogramming factors OCT3/4, SOX2, CMYC and KLF4 were delivered using a non-integrative methodology that involves the use of Sendai virus