Focused critical care echocardiography: training for pediatricians and pediatric intensivists in an intensive care unit

Introducción: La ecocardiografía, enfocada al cuidado crítico, se ha convertido hoy en día en una competencia necesaria del médico que labora en las unidades de cuidado intensivo pediátrico. Objetivo: Evaluar un programa de entrenamiento teórico y práctico, diseñado para explorar la adquisición de habilidades en la obtención de imágenes y algunas medidas ecocardiográficas sugeridas para la ecocardiografía enfocada al cuidado crítico. Materiales y métodos: Se realizó un entrenamiento teóricopráctico, de 26 horas de duración, por médicos pediatras y pediatras intensivistas, bajo la tutoría de un cardiólogo pediatra. El programa incluyó análisis cualitativos de las variables pertenecientes a las ventanas básicas y cuantitativos de la ecocardiografía enfocada al cuidado crítico. Resultados: Las diferencias significativas se presentaron entre tener conocimientos previos en ecocardiografía, que se asoció a un mejor puntaje en la ventana apical cuatro cámaras (media: 9,0 DE: 1,02 P=0,021), y mejor correlación con el cardiólogo pediatra en la medición de la funcionalidad del ventrículo izquierdo (media:92,2 DE:6,3 P=0,036). Conclusiones: Este programa de entrenamiento fue útil para el entrenamiento en nivel básico de la ecocardiografía enfocada al cuidado crítico, con un nivel óptimo en la adquisición de las principales ventanas ecocardiográficas, y para la toma de algunas medidas ecocardiográficas.Introduction: Focused critical care echocardiography (FCCE) has become a necessary competency for physicians working in pediatric intensive care units. Objective: To asses a theoretical and practical training program designed to explore skills acquisition for obtaining views and some echocardiographic measurements suggested for FCCE. Materials and methods: A 26-hour long theoretical and practical training for pediatricians and pediatric intensivists under the guidance of a pediatric cardiologist. The program included qualitative analysis of the variables pertaining to basic echocardiographic windows, and quantitative analysis of FCCE. Results: There were significant differences between having prior echocardiography knowledge, associated with a higher score in the 4-chamber apical window (mean: 9.0; standard deviation [SD]: 1.02; P=0.021), and better correlation with the pediatric cardiologist regarding left ventricular function measurements (mean: 92.02; SD: 6.3; P=0.036). Conclusion: The program was useful for basic level training in FCCE with an optimal level of acquisition of the main echocardiographic windows and some echocardiographic measurements

Composition-dependent in vitro apatite formation at mesoporous bioactive glass-surfaces quantified by solid-state NMR and powder XRD

Silicate-based bioactive glasses exhibit bone-bonding properties due to the formation of a hydroxycarbonate apatite (HCA) layer at the glass surface on its contact with living tissues. This bone-healing process is triggered by ionic exchange between the glass and the surrounding fluids and thereby depends on the glass composition. In this work, the HCA formation from three mesoporous bioactive glasses (MBGs) of different compositions immersed in a simulated body fluid (SBF) was monitored for variable time intervals between 15 minutes to 30 days. By utilizing two independent assessment techniques, solid-state P-31 NMR spectroscopy and powder X-ray diffraction (PXRD), we report the first quantitative assessment of the HCA growth (i.e., "in vitro bioactivity") from a bioactive glass: both techniques allow for monitoring the crystallization of the amorphous calcium phosphate (ACP) precursor into HCA, i.e., a profile of the relative ACP/HCA fractions of the biomimetic phosphate layer formed at each MBG surface and SBF-exposure period. The amount of HCA present in each solid specimen after the SBF treatment, as well as the composition of the remaining cation-depleted MBG phase, was determined from PXRD data in conjunction with measured concentrations of Ca, Si, and P in the solution. In contrast with previous findings from in vitro bioactivity assessments of the same MBG compositions, the HCA formation is herein observed to increase concurrently with the Ca and P contents of the MBG; these apparently different composition-bioactivity observations stem from a significantly lower MBG-loading in the SBF solution utilized herein. The results are discussed in relation to the general task of performing bioactivity testing in SBF, where we highlight the importance of adapting the concentration of the biomaterial to its composition to avoid perturbing the HCA crystallization and thereby altering the outcome of the test

Proton Environments in Biomimetic Calcium Phosphates Formed from Mesoporous Bioactive CaO-SiO2- P2O5 Glasses in vitro: Insights from Solid-State NMR

When exposed to body fluids, mesoporous bioactive glasses (MBGs) of the CaO{SiO2{P2O5 system develop a bone-bonding surface layer that initially consists of amorphous calcium phosphate(ACP), which transforms into hydroxy-carbonate apatite (HCA) with a very similar composition as bone/dentin mineral. Information from various 1H-based solid-state nuclear magnetic resonance (NMR) experiments were combined to elucidate the evolution of the proton speciations both at the MBG surface and within each ACP/HCA constituent of the biomimetic phosphate layer formed when each of three MBGs with distinct Ca, Si, and P contents was immersed in a simulated body fluid (SBF) for variable periods between 15 min and 30 days. Directly excited magic-angle-spinning (MAS) 1H NMR spectra mainly reflect the MBG component, whose surface is rich in water and silanol (SiOH) moieties. Double-quantum{single-quantum correlation 1H NMR experimentation at fast MAS revealed their interatomic proximities. The comparatively minor H species of each ACP and HCA component were probed selectively by heteronuclear 1H{31P NMR experimentation. The initially prevailing ACP phase comprises H2O and "non-apatitic" HPO2

Effects of short-term strength training on body composition, muscle strength and functional capacity of elderly: a systematic review and meta-analysis

To examine the effects of short-term strength training (STST) on different manifestations of muscle strength in the lower limbs, functional capacity and body composition of people 65 years old or older. We searched the electronic databases (PubMed, Web of Science and Cochrane) to identify all publications using STST (up to 12 weeks) in people aged 65 or older, published in the last five years, prior to May 2018. Results were analyzed as continuous data using random effects to calculate the standardized mean difference (SMD) and the 95% confidence interval (95%CI). 28 studies with 921 subjects met the inclusion criteria and were analyzed. These works were grouped into three categories for analysis: Muscular Strength, Functional Capacity and Body Composition. In Muscular Strength category, the overall pooled effect estimate was 0.95 (95%CI: 0.63; 1.26), with a significant STST effect (Z= 5.93; p<0.001), over the different strength manifestations analyzed. In Functional Capacity category, the STST decreased the Time Up-and-Go test run time (SMD: -1.01; 95%CI: -1.56; -0.47) and increased the repetitions’ number performed in 30-s chair-stand test (SMD: 1.07, 95% CI: 0.79, 1.34). In Body Composition category, the overall pooled effect estimate was 0.13 (95%CI: -0.16; 0.42), without finding a significant effect of STST (Z= 0.87; p= 0.38). STST has a moderate to large effect in improving the different manifestations of muscle strength and functional capacity. However, this type of intervention has no effect on body composition

Regulation of SCF TIR1/AFB E3 ligase assembly by S-nitrosylation of Arabidopsis SKP1-like1 impacts on auxin signaling

The F-box proteins (FBPs) TIR1/AFBs are the substrate recognition subunits of SKP1–cullin–F-box (SCF) ubiquitin ligase complexes and together with Aux/IAAs form the auxin co-receptor. Although tremendous knowledge on auxin perception and signaling has been gained in the last years, SCFTIR1/AFBs complex assembly and stabilization are emerging as new layers of regulation. Here, we investigated how nitric oxide (NO), through S-nitrosylation of ASK1 is involved in SCFTIR1/AFBs assembly. We demonstrate that ASK1 is S-nitrosylated and S-glutathionylated in cysteine (Cys) 37 and Cys118 residues in vitro. Both, in vitro and in vivo protein-protein interaction assays show that NO enhances ASK1 binding to CUL1 and TIR1/AFB2, required for SCFTIR1/AFB2 assembly. In addition, we demonstrate that Cys37 and Cys118 are essential residues for proper activation of auxin signaling pathway in planta. Phylogenetic analysis revealed that Cys37 residue is only conserved in SKP proteins in Angiosperms, suggesting that S-nitrosylation on Cys37 could represent an evolutionary adaption for SKP1 function in flowering plants. Collectively, these findings indicate that multiple events of redox modifications might be part of a fine-tuning regulation of SCFTIR1/AFBs for proper auxin signal transduction.This work has been supported by grants from CONICET (PIP 0202 to M.C.T), Universidad Nacional de Mar del Plata and Agencia Nacional de Promoción Científica y Tecnológica, -Argentina (PICT 1167 to M.C.T and PICT 2421 to C.A.C); from the Spanish Government (PS09/00101 and PI12/00875 to A.M.R., cofinanced by the European Union ERDF), and by the Argentinian-Spanish Integrated Action ES/11/02 / PRIAIBAR- 2011-0782. A.M.R. is supported by the I3SNS programme (ISCIII, Spanish Government). The Proteomics Service of the CBMSO is a member of Proteored (PRB2-ISCIII), and is supported by grants PT13/ 0001/0024 and PT17/0019/0018 of Spanish Government (cofinanced by the European Union ERDF). Work in the laboratory of M.E is supported by grants from the National Institutes of Health (NIH) GM43644, The Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation, US

Factores que inciden en la automedicación en el municipio de Rio Negro Antioquia

La automedicación se ha constituido en las últimas décadas, en una actitud muy errónea, cotidiana y habitual en la mayor parte de las personas adultas. Lo que ha hecho que se convierta en uno de los problemas de salud pública más graves que afectan a la población mundial. El uso irresponsable e inapropiado de medicamentos sin receta o prescritos con anterioridad que, por iniciativa y voluntad propia de la persona, trata de buscar alivio de una determinada enfermedad o síntoma sin que exista la debida supervisión de un profesional de salud, ocasionando daños y peligros a la vida, que en muchos casos son irreversibles y conducen a la muerte. El auto cuidado, es decir, el propio tratamiento de los signos y síntomas de enfermedad que las personas padecen, a lo largo de la historia de la humanidad ha sido la forma más utilizada para el mantenimiento de la salud. La automedicación, en la actualidad sigue siendo una práctica ampliamente extendida en la sociedad, fuertemente estimulada e inducida en gran medida por el aparato publicitario en los medios y en las vías públicas. El uso abusivo de los medicamentos es un importante problema, que las personas aún no toman en serio, siendo frecuente causa de efectos adversos.Self-medication has been constituted in the last decades, in a very erroneous, daily and habitual attitude in most of the adults. What has made it one of the most serious public health problems affecting the world's population. The irresponsible use of an over-the-counter or prescribed medication, which at the initiative and will of the person, seeks relief from a certain disease or symptom without the death of a health professional, causing harm and danger to life, which in many cases are irreversible and lead to death. Self-care, that is, the treatment of the signs and symptoms of the disease that people suffer, throughout the history of mankind has been the most used way to maintain health. Self-medication, at present, continues to be an extended practice in society, strongly stimulated and induced to a large extent by the advertising apparatus in the media and on public roads. The abusive use of medications is a major problem, which people still do not take seriously, which is a frequent cause of adverse effects

Adecuación de Normativa Vigente para la Contratación y Ejecución de Obras y Trabajos Públicos

Este proyecto tiene como propósito contar con un sistema de obras y trabajos públicos transparente y efectivo por el cual se garantice el correcto y adecuado uso de los recursos públicos destinados a la infraestructura universitaria.Fil: Izquierdo, Claudia María. Universidad Nacional de Córdoba. Secretaría de Planificación y Gestión Institucional. Subsecretaría de Planeamiento Físico; Argentina.Fil: Moré, Ricardo Rubén. Universidad Nacional de Córdoba. Secretaría de Planificación y Gestión Institucional. Subsecretaría de Planeamiento Físico; Argentina.Fil: Arias, Conrado. Universidad Nacional de Córdoba. Secretaría de Planificación y Gestión Institucional. Subsecretaría de Planeamiento Físico; Argentina.Fil: Cocco, María Fernanda. Universidad Nacional de Córdoba. Secretaría de Planificación y Gestión Institucional.Dirección General de Contrataciones. Argentina.Fil: López, Liliana. Universidad Nacional de Córdoba. Secretaría de Planificación y Gestión Institucional. Dirección General de Contrataciones. Argentina.Fil: Mazzoni, Emilio. Universidad Nacional de Córdoba. Secretaría de Planificación y Gestión Institucional. Dirección General de Contrataciones. Argentina.Fil: Boretto, Jhon. Universidad Nacional de Córdoba. Secretaría General; Argentina.Fil: Sigifredo, Eugenio. Universidad Nacional de Córdoba. Secretaría General. Dirección de Asuntos Jurídicos; Argentina.Fil: Obeide, Sergio F. Universidad Nacional de Córdoba. Secretaría de Planificación y Gestión Institucional; Argentina.Fil: Ponce, Isabel. Universidad Nacional de Córdoba. Secretaría de Planificación y Gestión Institucional. Subsecretaría de Planeamiento Físico; Argentina

Biological significance of monoallelic and biallelic BIRC3 loss in del(11q) chronic lymphocytic leukemia progression

Article number: 127[EN]BIRC3 is monoallelically deleted in up to 80% of chronic lymphocytic leukemia (CLL) cases harboring del(11q). In addition, truncating mutations in the remaining allele of this gene can lead to BIRC3 biallelic inactivation, which has been shown to be a marker for reduced survival in CLL. Nevertheless, the biological mechanisms by which these lesions could contribute to del(11q) CLL pathogenesis and progression are partially unexplored. We implemented the CRISPR/Cas9-editing system to generate isogenic CLL cell lines harboring del(11q) and/or BIRC3 mutations, modeling monoallelic and biallelic BIRC3 loss. Our results reveal that monoallelic BIRC3 deletion in del(11q) cells promotes non-canonical NF-κB signaling activation via RelB-p52 nuclear translocation, being these effects allelic dose-dependent and therefore further enhanced in del(11q) cells with biallelic BIRC3 loss. Moreover, we demonstrate ex vivo in primary cells that del(11q) cases including BIRC3 within their deleted region show evidence of non-canonical NF-κB activation which correlates with high BCL2 levels and enhanced sensitivity to venetoclax. Furthermore, our results show that BIRC3 mutations in del(11q) cells promote clonal advantage in vitro and accelerate leukemic progression in an in vivo xenograft model. Altogether, this work highlights the biological bases underlying disease progression of del(11q) CLL patients harboring BIRC3 deletion and mutation

Biological significance of monoallelic and biallelic BIRC3 loss in del(11q) chronic lymphocytic leukemia progression

© The Author(s) 2021.BIRC3 is monoallelically deleted in up to 80% of chronic lymphocytic leukemia (CLL) cases harboring del(11q). In addition, truncating mutations in the remaining allele of this gene can lead to BIRC3 biallelic inactivation, which has been shown to be a marker for reduced survival in CLL. Nevertheless, the biological mechanisms by which these lesions could contribute to del(11q) CLL pathogenesis and progression are partially unexplored. We implemented the CRISPR/Cas9-editing system to generate isogenic CLL cell lines harboring del(11q) and/or BIRC3 mutations, modeling monoallelic and biallelic BIRC3 loss. Our results reveal that monoallelic BIRC3 deletion in del(11q) cells promotes non-canonical NF-κB signaling activation via RelB-p52 nuclear translocation, being these effects allelic dose-dependent and therefore further enhanced in del(11q) cells with biallelic BIRC3 loss. Moreover, we demonstrate ex vivo in primary cells that del(11q) cases including BIRC3 within their deleted region show evidence of non-canonical NF-κB activation which correlates with high BCL2 levels and enhanced sensitivity to venetoclax. Furthermore, our results show that BIRC3 mutations in del(11q) cells promote clonal advantage in vitro and accelerate leukemic progression in an in vivo xenograft model. Altogether, this work highlights the biological bases underlying disease progression of del(11q) CLL patients harboring BIRC3 deletion and mutation.This work was supported by grants from the Spanish Fondo de Investigaciones Sanitarias PI15/01471, PI18/01500, Instituto de Salud Carlos III (ISCIII), European Regional Development Fund (ERDF) “Una manera de hacer Europa”, “Consejería de Educación, Junta de Castilla y León” (SA271P18), “Proyectos de Investigación del SACYL”, Spain GRS 2062/A/19, GRS 1847/A/18, GRS1653/A17,“Fundación Memoria Don Samuel Solórzano Barruso” (FS/23-2018), by grants (RD12/0036/0069) from Red Temática de Investigación Cooperativa en Cáncer (RTICC), Universidad de Salamanca (Programa XIII), Centro de Investigación Biomédica en Red de Cáncer (CIBERONC CB16/12/00233) and SYNtherapy “Synthetic Lethality for Personalized Therapy-based Stratification In Acute Leukemia” (ERAPERMED2018-275); ISCIII (AC18/00093), co-funded by ERDF/ESF, “Investing in your future”. M.Q.Á. and A.E.R.V. are supported with a research grant by FEHH (“Fundación Española de Hematología y Hemoterapia”); M.H.S. holds a Sara Borrell postdoctoral contract (CD19/00222) from the Instituto de Salud Carlos III (ISCIII). C.P.C. was supported by an “Ayuda predoctoral en Oncología” (AECC) and is a recipient of a PFIS grant (FI19/00191) from Instituto de Salud Carlos III; PFIS grant and Sara Borrell postdoctoral contrat are co-founded by Fondo Social Europeo (FSE) “El Fondo Social Europeo invierte en tu futuro”; J.L.O. and R.B.S. are supported by a grant from the University of Salamanca (“Contrato postdoctoral programa II”)

S-Nitrosation of E3 Ubiquitin Ligase Complex Components Regulates Hormonal Signalings in Arabidopsis

E3 ubiquitin ligases mediate the last step of the ubiquitination pathway in the ubiquitin-proteasome system (UPS). By targeting transcriptional regulators for their turnover, E3s play a crucial role in every aspect of plant biology. In plants, SKP1/CULLIN1/F-BOX PROTEIN (SCF)-type E3 ubiquitin ligases are essential for the perception and signaling of several key hormones including auxins and jasmonates (JAs). F-box proteins, TRANSPORT INHIBITOR RESPONSE 1 (TIR1) and CORONATINE INSENSITIVE 1 (COI1), bind directly transcriptional repressors AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) and JASMONATE ZIM-DOMAIN (JAZ) in auxin- and JAs-depending manner, respectively, which permits the perception of the hormones and transcriptional activation of signaling pathways. Redox modification of proteins mainly by S-nitrosation of cysteines (Cys) residues via nitric oxide (NO) has emerged as a valued regulatory mechanism in physiological processes requiring its rapid and versatile integration. Previously, we demonstrated that TIR1 and Arabidopsis thaliana SKP1 (ASK1) are targets of S-nitrosation, and these NO-dependent posttranslational modifications enhance protein-protein interactions and positively regulate SCFTIR1 complex assembly and expression of auxin response genes. In this work, we confirmed S-nitrosation of Cys140 in TIR1, which was associated in planta to auxin-dependent developmental and stress-associated responses. In addition, we provide evidence on the modulation of the SCFCOI1 complex by different S-nitrosation events. We demonstrated that S-nitrosation of ASK1 Cys118 enhanced ASK1-COI1 protein-protein interaction. Overexpression of non-nitrosable ask1 mutant protein impaired the activation of JA-responsive genes mediated by SCFCOI1 illustrating the functional relevance of this redox-mediated regulation in planta. In silico analysis positions COI1 as a promising S-nitrosation target, and demonstrated that plants treated with methyl JA (MeJA) or S-nitrosocysteine (NO-Cys, S-nitrosation agent) develop shared responses at a genome-wide level. The regulation of SCF components involved in hormonal perception by S-nitrosation may represent a key strategy to determine the precise time and site-dependent activation of each hormonal signaling pathway and highlights NO as a pivotal molecular player in these scenarios.Fil: Terrile, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Tebez, Nuria Malena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Colman, Silvana Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Mateos, Julieta Lisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Morato López, Esperanza. CENTRO DE BIOLOGIA MOLECULAR SEVERO OCHOA (CBMSO) ; UNIVERSIDAD AUTONOMA DE MADRID;Fil: Sánchez López, Nuria. CENTRO DE BIOLOGIA MOLECULAR SEVERO OCHOA (CBMSO) ; UNIVERSIDAD AUTONOMA DE MADRID;Fil: Izquierdo Álvarez, Alicia. No especifíca;Fil: Marina, Anabel. CENTRO DE BIOLOGIA MOLECULAR SEVERO OCHOA (CBMSO) ; UNIVERSIDAD AUTONOMA DE MADRID;Fil: Calderón Villalobos, Luz Irina A.. Donald Danforth Plant Science Center; Estados UnidosFil: Estelle, Mark. No especifíca;Fil: Martínez Ruiz, Antonio. No especifíca;Fil: Fiol, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Casalongue, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Iglesias, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentin