128 research outputs found
Information on antiprotonic atoms and the nuclear periphery from the PS209 experiment
In the PS209 experiments at CERN two kinds of measurements were performed:
the in-beam measurement of X-rays from antiprotonic atoms and the
radiochemical, off-line determination of the yield of annihilation products
with mass number A_t -1 (less by 1 than the target mass). Both methods give
observables which allows to study the peripheral matter density composition and
distribution.Comment: LaTeX (espcrc1 style), 6 pages, 3 EPS figures, 1 table, Proceedings
of the Sixth Biennal Conference on Low-Energy Antiproton Physics LEAP 2000,
Venice, Ital
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SUSD2 expression in high-grade serous ovarian cancer correlates with increased patient survival and defective mesothelial clearance
The cause of death among the majority of epithelial ovarian cancer (EOC) patients involves passive dissemination of cancer cells within the peritoneal cavity and subsequent implantation of cancer spheroids into adjacent organs. Thus, it is important to identify the factors that mediate EOC metastasis and implantation, including clearance of the mesothelium. Sushi domain containing 2 (SUSD2) encodes a type I transmembrane protein containing several functional domains inherent to adhesion molecules. Immunohistochemical analysis determined the presence of SUSD2 in several subtypes of EOC, with the strongest staining observed in high-grade serous ovarian carcinomas (HGSOCs). A high-density, clinically annotated HGSOC tissue microarray was stained with an anti-SUSD2 antibody. Patients with tumors that had a low percentage of SUSD2 staining cells had a shorter median survival (31.7 months) compared with patients who had tumors with extensive SUSD2 staining (49.1 months; P-value=0.0083). To investigate the role of SUSD2 in HGSOCs, stable OVCAR3, OVSAHO and KURAMOCHI cell lines were established with knockdown (KD) or non-targeting (NT) of SUSD2. Boyden chamber and wound-healing assays demonstrated that OVCAR3, OVSAHO and KURAMOCHI SUSD2-KD cells migrated at significantly higher rates compared with their SUSD2 NT counterpart cell lines. Quantitative reverse transcription–PCR and western immunoblot analysis indicated an inverse relationship between SUSD2 and well-characterized mesenchymal proteins, including Twist-1, Zeb-1, N-cadherin, STEAP1, AHNAK, Snail-1, COL5A2 and Snail-3 in OVCAR3, OVSAHO and KURAMOCHI cell line models. In addition, OVCAR3 and KURAMOCHI SUSD2-KD spheroids displayed increased mesothelial clearance ability compared with cells that express endogenous levels of SUSD2. These data suggest that SUSD2 has a role in the inhibition of mesothelial clearance, which is required for metastasis. Altogether, our findings indicate that SUSD2 impedes migration, epithelial-to-mesenchymal transitional and mesothelial clearance of HGSOC cells, consistent with prolonged survival of patients with SUSD2-expressing tumors
Coulomb excitation of 73Ga
The B(E2; Ii -> If) values for transitions in 71Ga and 73Ga were deduced from
a Coulomb excitation experiment at the safe energy of 2.95 MeV/nucleon using
post-accelerated beams of 71,73Ga at the REX-ISOLDE on-line isotope mass
separator facility. The emitted gamma rays were detected by the
MINIBALL-detector array and B(E2; Ii->If) values were obtained from the yields
normalized to the known strength of the 2+ -> 0+ transition in the 120Sn
target. The comparison of these new results with the data of less neutron-rich
gallium isotopes shows a shift of the E2 collectivity towards lower excitation
energy when adding neutrons beyond N = 40. This supports conclusions from
previous studies of the gallium isotopes which indicated a structural change in
this isotopical chain between N = 40 and N = 42. Combined with recent
measurements from collinear laser spectroscopy showing a 1/2- spin and parity
for the ground state, the extracted results revealed evidence for a 1/2-; 3/2-
doublet near the ground state in 73 31Ga42 differing by at most 0.8 keV in
energy
The deubiquitinating enzyme USP17 is essential for GTPase subcellular localization and cell motility
Deubiquitinating enzymes are now emerging as potential therapeutic targets that control many cellular processes, but few have been demonstrated to control cell motility. Here, we show that ubiquitin-specific protease 17 (USP17) is rapidly and transiently induced in response to chemokines SDF-1/CXCL12 and IL-8/CXCL8 in both primary cells and cell lines, and that its depletion completely blocks chemokine-induced cell migration and cytoskeletal rearrangements. Using live cell imaging, we demonstrate that USP17 is required for both elongated and amoeboid motility, in addition to chemotaxis. USP17 has previously been reported to disrupt Ras localization and we now find that USP17 depletion blocks chemokine-induced subcellular relocalization of GTPases Cdc42, Rac and RhoA, which are GTPases essential for cell motility. Collectively, these results demonstrate that USP17 has a critical role in cell migration and may be a useful drug target for both inflammatory and metastatic disease
Collectivity in Pb-196,Pb-198 isotopes probed in Coulomb-excitation experiments at REX-ISOLDE
The neutron-deficient Pb-196,Pb-198 isotopes have been studied in Coulomb-excitation experiments employing the Miniball gamma-ray spectrometer and radioactive ion beams from the REX-ISOLDE post-accelerator at CERN. The reduced transition probabilities of the first excited 2(+) states in Pb-196 and Pb-198 nuclei have been measured for the first time. Values of B (E2) = 18.2(-4.1)(+4.8) W. u. and B (E2) = 13.1(-3.5)(+4.9) W. u., were obtained, respectively. The experiment sheds light on the development of collectivity when moving from the regime governed by the generalised seniority scheme to a region, where intruding structures, associated with different deformed shapes, start to come down in energy and approach the spherical ground state.Peer reviewe
Safety and outcome of definitive chemoradiotherapy in elderly patients with oesophageal cancer
Little is known about chemoradiotherapy (CRT) in elderly patients with a locally advanced oesophageal cancer (OC). The aim of our study was to evaluate the tolerance and the outcome of elderly patients older than 70 years treated with CRT for a non-metastatic OC. Chemoradiotherapy was based on radiotherapy combined with a cisplatin-based chemotherapy. Clinical complete response (CCR) to CRT was evaluated on upper digestive endoscopy and computed tomography scan 6–8 weeks after CRT completion. One hundred and nine consecutive patients were included. A CCR was observed in 63 patients (57.8%) and 2-year survival was 35.5%. Adverse events ⩾grade 3 were observed in 26 (23.8%) patients. Chemotherapy dose reduction, chemotherapy delays more than 1 week, and treatment discontinuation were observed in 33 (30.3%), 45 (41.3%), and 17 patients (15.6%), respectively. Comorbidity index according to Charlson score was significantly associated with treatment tolerance. In multivariate analysis, a CCR to CRT (P<0.01), a dose of radiotherapy ⩾80% (P=0.02), and a Charlson score ⩽2 (P=0.046) were identified as independent prognostic factors of overall survival. These results suggest that CRT could be considered as an effective treatment without major toxicity in elderly patients with OC
Study of the onset of deformation and shape coexistence in Ar via the inverse kinematics () reaction
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