An Evidence Based Practice Guide for Parents and Schools: Supporting Kids and Adolescents\u27 Mental Health During and After COVID-19 Pandemic

Adolescent mental health is important to consider by parents and schools. Parents need to build a strengthened mentality in their children and with the school support, the results would be greater. The active participation of schools is needed in order to support kids\u27 and adolescents\u27 mental health and create parent awareness about mental health. Also, when parents are able to identify early signs of mental health problems, they can prevent it from becoming greater and the school can help parents learn about these early signs. In addition, parents play a fundamental role in identifying symptoms of mental health problems in their kids and it can make a big difference whether parents seek help when they show signs and symptoms or have suspicions of possible mental disorders. If the parents engage with the treatment of their children, they can be better informed, reinforce the work done by the therapist at home and be a more effective support for the adolescent

La bella Italia

Las diferentes gastronomías en el mundo hacen que el cocinar sea una experiencia nueva cada vez. La pasión por la cocina es algo con lo que se nace, y que en un largo camino de aprendizaje se va mejorando y perfeccionando. Saber cocinar es un arte que ofrece deleitar a las personas con las comidas más exquisitas y deliciosas El proyecto que se presentará a continuación nos lleva por un recorrido por las diferentes regiones de Italia, dividiéndola en norte, centro y sur. Basándose en la exquisita gastronomía italiana tradicional, se presentó en el restaurante Marcus un menú llamado “La bella Italia”, comenzando con un antipasto del centro, una entrada del sur, un plato fuerte del norte y finamente un postre trilogía que une a estas tres regiones. En el siguiente texto, se observará el proceso para la creación del menú, los utensilios utilizados, la historia de los platos presentados y el resultado exitoso que representó

Host-Seeking Activity of Bluetongue Virus Vectors: Endo/Exophagy and Circadian Rhythm of Culicoides in Western Europe

Feeding success of free-living hematophagous insects depends on their ability to be active when hosts are available and to reach places where hosts are accessible. When the hematophagous insect is a vector of pathogens, determining the components of host-seeking behavior is of primary interest for the assessment of transmission risk. Our aim was to describe endo/exophagy and circadian host-seeking activity of Palaearctic Culicoides species, which are major biting pests and arbovirus vectors, using drop traps and suction traps baited with four sheep, as bluetongue virus hosts. Collections were carried out in the field, a largely-open stable and an enclosed stable during six collection periods of 24 hours in April/May, in late June and in September/October 2010 in western France. A total of 986 Culicoides belonging to 13 species, mainly C. brunnicans and C. obsoletus, was collected on animal baits. Culicoides brunnicans was clearly exophagic, whereas C. obsoletus was able to enter stables. Culicoides brunnicans exhibited a bimodal pattern of host-seeking activity with peaks just after sunrise and sunset. Culicoides obsoletus was active before sunset in spring and autumn and after sunset in summer, thus illustrating influence of other parameters than light, especially temperature. Description of host-seeking behaviors allowed us to discuss control strategies for transmission of Culicoides-borne pathogens, such as bluetongue virus. However, practical vector-control recommendations are difficult to provide because of the variation in the degree of endophagy and time of host-seeking activity.This study was funded by CIRAD, by the Ministe`re de l’agriculture, de l’alimentation, de la peˆche, de la ruralite´ et de l’ame´nagement du terroire and by the EU FP7-HEALTH-2010-single-stage grant 261504 EDENext. This paper is catalogued by the EDENext Steering Committee as EDENext032 (http://www.edenext. eu). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. No additional external funding received for this study

The 'PUCE CAFE' Project: the First 15K Coffee Microarray, a New Tool for Discovering Candidate Genes correlated to Agronomic and Quality Traits

Background: Understanding the genetic elements that contribute to key aspects of coffee biology will have an impact on future agronomical improvements for this economically important tree. During the past years, EST collections were generated in Coffee, opening the possibility to create new tools for functional genomics. Results: The "PUCE CAFE" Project, organized by the scientific consortium NESTLE/IRD/CIRAD, has developed an oligo-based microarray using 15,721 unigenes derived from published coffee EST sequences mostly obtained from different stages of fruit development and leaves in Coffea Canephora (Robusta). Hybridizations for two independent experiments served to compare global gene expression profiles in three types of tissue matter (mature beans, leaves and flowers) in C. canephora as well as in the leaves of three different coffee species (C. canephora, C. eugenoides and C. arabica). Microarray construction, statistical analyses and validation by Q-PCR analysis are presented in this study. Conclusion: We have generated the first 15 K coffee array during this PUCE CAFE project, granted by Genoplante (the French consortium for plant genomics). This new tool will help study functional genomics in a wide range of experiments on various plant tissues, such as analyzing bean maturation or resistance to pathogens or drought. Furthermore, the use of this array has proven to be valid in different coffee species (diploid or tetraploid), drastically enlarging its impact for high-throughput gene expression in the community of coffee research

“diseño de proyectos de voz sobre ip” diseño e implementación de un sistema de telefonía ip para “uees”

La Universidad de Especialidades Espíritu Santo (UESS) es una empresa privada que en la actualidad cuenta con un sistema de red telefónico común, se encuentra interesada en mejorar sus prestaciones de servicio de comunicación y acceso a la información, se requiere implementar un servicio de Voz Ip, que optimizará la transferencia de voz y la transferencia de datos con un buen servicio. Para este proyecto se empleará equipos de última tecnología, permitiendo establecer llamadas de voz y fax sobre conexiones IP obteniendo de esta manera una reducción de costos considerables en telefonía

Hyper sensitive protein detection by Tandem-HTRF reveals Cyclin D1 dynamics in adult mouse

International audienceWe present here a novel method for the semi-quantitative detection of low abundance proteins in solution that is both fast and simple. It is based on Homogenous Time Resolved Förster Resonance Energy Transfer (HTRF), between a lanthanide labeled donor antibody and a d2 or XL665 labeled acceptor antibody that are both raised against different epitopes of the same target. This novel approach we termed "Tandem-HTRF", can specifically reveal rare polypeptides from only a few microliters of cellular lysate within one hour in a 384-well plate format. Using this sensitive approach, we observed surprisingly that the core cell cycle regulator Cyclin D1 is sustained in fully developed adult organs and harbors an unexpected expression pattern affected by environmental challenge. Thus our method, Tandem-HTRF offers a promising way to investigate subtle variations in the dynamics of sparse proteins from limited biological material

Toward identification of TAL effector targeted susceptibility genes in black rot of cabbage caused by Xanthomonas campestris pv. campestris

International audienc

TAG-RNAi overcomes off-target effects in cancer models

International audienceRNA interference offers therapeutic opportunities for the clinical targeting of otherwise undruggable oncogenes. However RNAi can have off-target effects that considerably increase treatment risks. To manage these side effects and allow an easy subtraction of their activity in healthy tissues, we present here the TAG-RNAi approach where cells that are not designated targets do not have the mRNA tag. Using TAG-RNAi we first established the off-target signatures of three different siRNAs specific to the Cyclin D1 oncogene by RNA-sequencing of cultured cancer cells expressing a FLAG-HA-tagged-Cyclin D1. Then, by symmetrical allografts of tagged-cancer cells and untagged controls on the left and right flanks of model mice, we demonstrate that TAG-RNAi is a reliable approach to study the functional impact of any oncogene without off-target bias. Finally we show, as examples, that mutation-specific TAG-RNAi can be applied to downregulate two oncogenic mutants, KRAS-G12V or BRAF-V600E, while sparing the expression of the wild-type proteins. TAG-RNAi will thus avoid the traditional off-target limitations of RNAi in future experimental approaches

Host-seeking activity of bluetongue virus vectors: endo/exophagy and circadian rhythm of Culicoides in Western Europe.

Feeding success of free-living hematophagous insects depends on their ability to be active when hosts are available and to reach places where hosts are accessible. When the hematophagous insect is a vector of pathogens, determining the components of host-seeking behavior is of primary interest for the assessment of transmission risk. Our aim was to describe endo/exophagy and circadian host-seeking activity of Palaearctic Culicoides species, which are major biting pests and arbovirus vectors, using drop traps and suction traps baited with four sheep, as bluetongue virus hosts. Collections were carried out in the field, a largely-open stable and an enclosed stable during six collection periods of 24 hours in April/May, in late June and in September/October 2010 in western France. A total of 986 Culicoides belonging to 13 species, mainly C. brunnicans and C. obsoletus, was collected on animal baits. Culicoides brunnicans was clearly exophagic, whereas C. obsoletus was able to enter stables. Culicoides brunnicans exhibited a bimodal pattern of host-seeking activity with peaks just after sunrise and sunset. Culicoides obsoletus was active before sunset in spring and autumn and after sunset in summer, thus illustrating influence of other parameters than light, especially temperature. Description of host-seeking behaviors allowed us to discuss control strategies for transmission of Culicoides-borne pathogens, such as bluetongue virus. However, practical vector-control recommendations are difficult to provide because of the variation in the degree of endophagy and time of host-seeking activity