DESENVOLVIMENTO DE MÉTODO PARA DETERMINAÇÃO DE CHUMBO EM AMOSTRAS DE BATOM

Sendo o quarto maior mercado consumidor de produtos de higiene pessoal, perfumaria e cosméticos com uma movimentação de US$ 229 bilhões, o Brasil representa 4,3% do mercado mundial, ficando atrás apenas de Estados Unidos, China e Japão. Mesmo com a retração econômica do real de -5,6% no ano de 2021 houve um aumento no volume de vendas deste setor de 3,4% em relação ao ano de 2020, sendo o segundo país que mais lança produtos anualmente (ABIHPEC, 2022)

DETERMINAÇÃO DE CLORO RESIDUAL EMPREGANDO IMAGENS DIGITAIS

Com o aumento da população e sua concentração em grandes cidades, os sistemas de distribuição e desinfecção de água se tornaram cada vez mais complexos e necessários. A poluição hídrica exige o desenvolvimento de métodos para tornar a água potável, sendo um dos mais utilizados a cloração, que consiste na adição de cloro como agente desinfectante que inativa e destrói organismos nocivos (BARROS, 2018)

CARACTERIZAÇÃO DOS TEORES DE DE METAIS TÓXICOS EM AMOSTRAS DE SEDIMENTOS DO RIO JOANES, BAHIA

Os sedimentos desempenham um papel importante na detecção de substâncias nocivas insolúveis lançadas em ambiente aquáticos, pois são considerados como destino final de diversos contaminantes. São reconhecidos também como transportadores e possíveis fontes de contaminação, já que tal compartimento ambiental pode liberar espécies contaminantes (LIMA et al., 2001)

Erythrina velutina Willd. alkaloids : piecing biosynthesis together from transcriptome analysis and metabolite profiling of seeds and leaves

Introduction: Natural products of pharmaceutical interest often do not reach the drug market due to the associated low yields and difficult extraction. Knowledge of biosynthetic pathways is a key element in the development of biotechnological strategies for plant specialized metabolite production. Erythrina species are mainly used as central nervous system depressants in folk medicine and are important sources of bioactive tetracyclic benzylisoquinoline alkaloids (BIAs), which can act on several pathology-related biological targets. Objectives: In this sense, in an unprecedented approach used with a non-model Fabaceae species grown in its unique arid natural habitat, a combined transcriptome and metabolome analyses (seeds and leaves) is presented. Methods: The Next Generation Sequencing-based transcriptome (de novo RNA sequencing) was carried out in a NextSeq 500 platform. Regarding metabolite profiling, the High-resolution Liquid Chromatography was coupled to DAD and a micrOTOF-QII mass spectrometer by using electrospray ionization (ESI) and Time of Flight (TOF) analyzer. The tandem MS/MS data were processed and analyzed through Molecular Networking approach. Results: This detailed macro and micromolecular approach applied to seeds and leaves of E. velutina revealed 42 alkaloids, several of them unique. Based on the combined evidence, 24 gene candidates were put together in a putative pathway leading to the singular alkaloid diversity of this species. Conclusion: Overall, these results could contribute by indicating potential biotechnological targets formodulation of erythrina alkaloids biosynthesis as well as improve molecular databases with omic data from a non-model medicinal plant, and reveal an interesting chemical diversity of Erythrina BIA harvested in Caatinga

Erythrina velutina Willd. alkaloids: Piecing biosynthesis together from transcriptome analysis and metabolite profiling of seeds and leaves

Introduction: Natural products of pharmaceutical interest often do not reach the drug market due to the associated low yields and difficult extraction. Knowledge of biosynthetic pathways is a key element in the development of biotechnological strategies for plant specialized metabolite production. The scarce studies regarding non-model plants impair advances in this field. Erythrina spp. are mainly used as central nervous system depressants in folk medicine and are important sources of bioactive tetracyclic benzylisoquinoline alkaloids, which can act on several pathology-related biological targets. Objective: Herein the purpose is to employ combined transcriptome and metabolome analyses (seeds and leaves) of a non-model medicinal Fabaceae species grown in its unique arid natural habitat. The study tries to propose a putative biosynthetic pathway for the bioactive alkaloids by using an omic integrated approach. Methods: The Next Generation Sequencing-based transcriptome (de novo RNA sequencing) was carried out in a Illumina NextSeq 500 platform. Regarding the targeted metabolite profiling, Nuclear Magnetic Resonance and the High-Performance Liquid Chromatography coupled to a micrOTOF-QII, High Resolution Mass Spectrometer, were used. Results: This detailed macro and micromolecular approach applied to seeds and leaves of E. velutina revealed 42 alkaloids by metabolome tools. Based on the combined evidence, 24 gene candidates were put together in a putative pathway leading to the singular alkaloid diversity of this species. Conclusion: These results contribute by indicating potential biotechnological targets Erythrina alkaloids biosynthesis as well as to improve molecular databases with omic data from a non-model medicinal plant. Furthermore, they reveal an interesting chemical diversity in Erythrina velutina harvested in Caatinga. Last, but not least, this data may also contribute to tap Brazilian biodiversity in a rational and sustainable fashion, promoting adequate public policies for preservation and protection of sensitive areas within the Caatinga

DETERMINAÇÃO E CARACTERIZAÇÃO DOS TEORES DE METAIS E METALÓIDES EM MACRÓFITAS AQUÁTICAS ORIUNDAS DO RIO JOANES, BAHIA

Macrófita aquática (aquatic macrophytes ou macrophytes) é um termo utilizado para designar o conjunto de vegetais adaptados ao ambiente aquático. Dentre elas, destacamos o Aguapé (Eichhornia crassipes), uma planta aquática encontrada em regiões tropicais (ALVES et. al). Esse tipo de planta vem manifestando uma alta capacidade de absorver metais e metalóides segundo alguns estudos. (MEES, J. B. R)