Participatory research in complex marine conservation settings: A review of recent trends and lessons for the future

The conservation of marine ecosystems and species is inherently complex, plagued by social, political, economic and ecological uncertainty. Navigating these challenges to sustain marine systems requires the understanding, consideration and uptake of multiple knowledge systems within decision-making processes. Participatory research approaches, such as knowledge co-production, are advocated given their potential to generate knowledge with those who are most affected by the issue at hand and, based on that knowledge, develop and implement meaningful conservation practices collaboratively. In doing so participatory research is positioned to make marine research more democratic and impactful, and as such, has been a field of growing research enquiry. The aim of this study, therefore, is to synthesise (via a systematic scoping review) the existing literature to generate guidance that can help researchers and practitioners plan and apply participatory research approaches in a complex marine context. Results from systematically identifying and analyzing 337 articles show that the implementation of participatory research approaches are most common in the global north. Participatory research approaches most frequently included representatives from government, industry and civil society (e.g. community groups), while indigenous groups and communities were rarely included. A diversity of participatory research approaches have been used in relation to marine conservation in different settings and at various stages of the participatory process, each with their own challenges and benefits, that can lead to either positive outcomes for actors (e.g. social learning) or negative outcomes (e.g. disempowerment). We draw on our findings to provide guidance for improving the implementation of participatory research approaches in marine conservation such as taking the time to understand context before commencing participatory research approaches, and ensuring transparency with all actors throughout the process. We highlight key implications for researcher and practitioners implementing participatory research approaches including the importance of a diversity of approaches and methods to ensure access, supporting two way communication, addressing power imbalances, and the need to recognise, report on and address pre-existing barriers. Many of these require greater investment in terms of money or personnel, and new funding models are needed with a focus on longevity and sustainability

The role of visual framing in marine conservation communication

Marine conservation programs face significant challenges engaging different audiences to participate in conservation actions. While it is understood that images can play a crucial role in shaping people's behaviours, in the context of marine conservation, research has focused on the effect of words. We know little about the effect of images on influencing people's intentions. Thus, there is scant guidance for marine conservation programs wanting to use images to improve communication and engagement strategies. This study addresses this gap by assessing how images are used in social media in Australian turtle conservation programs. Specifically, using visual framing analysis and online surveys, we assessed whether images of conservation actions or conservation threats increase participants emotions, self-efficacy, and intentions to engage in pro-conservation behaviours, using flatback turtles as a case study. We find that 1) images of animals are commonly used on social media, with certain types of images (e.g. landscapes, conservation actions) generating higher user engagement; 2) images on Twitter and Instagram generated higher user engagement than on Facebook; 3) images of conservation actions increased feelings of hope and self-efficacy, while images of conservation threats increased feelings of fear and reduced self-efficacy; and 4) feelings of self-efficacy increased participants' intentions to support low-effort conservation actions. Results suggest that the types of images posted on social media can influence viewers' emotions and intentions to engage in pro-conservation behaviours. Our findings provide guidance for conservation programs and managers to develop improved communication and engagement strategies through visual media

Clinical predictors of rectal lymphogranuloma venereum infection: results from a multicentre case-control study in the UK

Objective: Since 2003, over 2000 cases of lymphogranuloma venereum (LGV) have been diagnosed in the UK in men who have sex with men (MSM). Most cases present with proctitis, but there are limited data on how to differentiate clinically between LGV and other pathology. We analysed the clinical presentations of rectal LGV in MSM to identify clinical characteristics predictive of LGV proctitis and produced a clinical prediction model. Design: A prospective multicentre case–control study was conducted at six UK hospitals from 2008 to 2010. Cases of rectal LGV were compared with controls with rectal symptoms but without LGV. Methods: Data from 98 LGV cases and 81 controls were collected from patients and clinicians using computer-assisted self-interviews and clinical report forms. Univariate and multivariate logistic regression was used to compare symptoms and signs. Clinical prediction models for LGV were compared using receiver operating curves. Results: Tenesmus, constipation, anal discharge and weight loss were significantly more common in cases than controls. In multivariate analysis, tenesmus and constipation alone were suggestive of LGV (OR 2.98, 95% CI 0.99 to 8.98 and 2.87, 95% CI 1.01 to 8.15, respectively) and that tenesmus alone or in combination with constipation was a significant predictor of LGV (OR 6.97, 95% CI 2.71 to 17.92). The best clinical prediction was having one or more of tenesmus, constipation and exudate on proctoscopy, with a sensitivity of 77% and specificity of 65%. Conclusions: This study indicates that tenesmus alone or in combination with constipation makes a diagnosis of LGV in MSM presenting with rectal symptoms more likely

Is chlamydia screening and testing in Britain reaching young adults at risk of infection? Findings from the third National Survey of Sexual Attitudes and Lifestyles (Natsal-3)

In the context of widespread opportunistic chlamydia screening among young adults, we aimed to quantify chlamydia testing and diagnosis among 16-24 year olds in Britain in relation to risk factors for prevalent chlamydia infection

Effect of Audiovisual Training on Monaural Spatial Hearing in Horizontal Plane

The article aims to test the hypothesis that audiovisual integration can improve spatial hearing in monaural conditions when interaural difference cues are not available. We trained one group of subjects with an audiovisual task, where a flash was presented in parallel with the sound and another group in an auditory task, where only sound from different spatial locations was presented. To check whether the observed audiovisual effect was similar to feedback, the third group was trained using the visual feedback paradigm. Training sessions were administered once per day, for 5 days. The performance level in each group was compared for auditory only stimulation on the first and the last day of practice. Improvement after audiovisual training was several times higher than after auditory practice. The group trained with visual feedback demonstrated a different effect of training with the improvement smaller than the group with audiovisual training. We conclude that cross-modal facilitation is highly important to improve spatial hearing in monaural conditions and may be applied to the rehabilitation of patients with unilateral deafness and after unilateral cochlear implantation

Deciphering the functional role of spatial and temporal muscle synergies in whole-body movements

International audienceVoluntary movement is hypothesized to rely on a limited number of muscle synergies, the recruitment of which translates task goals into effective muscle activity. In this study, we investigated how to analytically characterize the functional role of different types of muscle synergies in task performance. To this end, we recorded a comprehensive dataset of muscle activity during a variety of whole-body pointing movements. We decomposed the electromyographic (EMG) signals using a space-by-time modularity model which encompasses the main types of synergies. We then used a task decoding and information theoretic analysis to probe the role of each synergy by mapping it to specific task features. We found that the temporal and spatial aspects of the movements were encoded by different temporal and spatial muscle synergies, respectively, consistent with the intuition that there should a correspondence between major attributes of movement and major features of synergies. This approach led to the development of a novel computational method for comparing muscle synergies from different participants according to their functional role. This functional similarity analysis yielded a small set of temporal and spatial synergies that describes the main features of whole-body reaching movements

Positive Selection Results in Frequent Reversible Amino Acid Replacements in the G Protein Gene of Human Respiratory Syncytial Virus

Human respiratory syncytial virus (HRSV) is the major cause of lower respiratory tract infections in children under 5 years of age and the elderly, causing annual disease outbreaks during the fall and winter. Multiple lineages of the HRSVA and HRSVB serotypes co-circulate within a single outbreak and display a strongly temporal pattern of genetic variation, with a replacement of dominant genotypes occurring during consecutive years. In the present study we utilized phylogenetic methods to detect and map sites subject to adaptive evolution in the G protein of HRSVA and HRSVB. A total of 29 and 23 amino acid sites were found to be putatively positively selected in HRSVA and HRSVB, respectively. Several of these sites defined genotypes and lineages within genotypes in both groups, and correlated well with epitopes previously described in group A. Remarkably, 18 of these positively selected tended to revert in time to a previous codon state, producing a “flip-flop” phylogenetic pattern. Such frequent evolutionary reversals in HRSV are indicative of a combination of frequent positive selection, reflecting the changing immune status of the human population, and a limited repertoire of functionally viable amino acids at specific amino acid sites

Structure-based drug discovery for combating influenza virus by targeting the PA?PB1 interaction

Influenza virus infections are serious public health concerns throughout the world. The development of compounds with novel mechanisms of action is urgently required due to the emergence of viruses with resistance to the currently-approved anti-influenza viral drugs. We performed in silico screening using a structure-based drug discovery algorithm called Nagasaki University Docking Engine (NUDE), which is optimised for a GPU-based supercomputer (DEstination for Gpu Intensive MAchine; DEGIMA), by targeting influenza viral PA protein. The compounds selected by NUDE were tested for anti-influenza virus activity using a cell-based assay. The most potent compound, designated as PA-49, is a medium-sized quinolinone derivative bearing a tetrazole moiety, and it inhibited the replication of influenza virus A/WSN/33 at a half maximal inhibitory concentration of 0.47?μM. PA-49 has the ability to bind PA and its anti-influenza activity was promising against various influenza strains, including a clinical isolate of A(H1N1)pdm09 and type B viruses. The docking simulation suggested that PA-49 interrupts the PA?PB1 interface where important amino acids are mostly conserved in the virus strains tested, suggesting the strain independent utility. Because our NUDE/DEGIMA system is rapid and efficient, it may help effective drug discovery against the influenza virus and other emerging viruses