Effect of peppermint (Mentha piperita) supplementation on carcass yield, meat taste, heart weight, liver weight and some blood parameters in laying quail (Coturnix Coturnix Japonica) (2)

This study was carried out to determine the effects of peppermint (Mentha Piperita) on blood parameters (cholesterol, triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL), malondialdehyte (MDA), antioxidant activity (AOA), total protein, glucose, carcass yield, heart, liver weights and organoleptic control of the carcasses in laying quails. A total of 180 15 week old laying quails were used in the study. There were 5 experimental groups which were supplemented with different ratios of Peppermint up to 5%. The control group received no peppermint supplements. The experiment continued for 10 weeks. Antioxidant activity and HDL amounts increased (P0.05) on the carcass quality with a 5 % peppermint supplementation to the diet. In conclusion, the supplementation of peppermint to diets up to a 5% level was found to be quite beneficial in terms of blood parameters such as MDA, AOA, LDL, and HDL

The Effects of Babesiosis on Oxidative Stress and DNA Damage in Anatolian Black Goats Naturally Infected with Babesia ovis

  Background: A reactive oxygen and nitrogen intermediate produced during an inflammatory response is the important part of host-defense strategies of organ-isms to kill the parasite. However, it is not well known whether these intermediates cause DNA damage and oxidative stress in goats infected with Babesia ovis. The pur-pose of this study was to clarify the effects of babesiosis on basal levels of DNA damage and oxidative status of goats naturally infected with B.ovis. Methods: DNA damage and antioxidant parameters were determined in B. ovis infected goats. Ten infected Anatolian Black Goats with B. ovis diagnosed via clinical signs and microscopic findings and ten healthy were used in the study. Results: The Babesia infection increased the levels of DNA damage, malondialde-hyde (MDA), protein carbonyl content (PCO) and plasma concentration of nitric oxide metabolites (NOx), and decreased total antioxidant activities (AOA) and re-duced glutathione (GSH). A significant positive correlation between DNA damage, MDA, PCO, and NOx concentrations was found in the infected goats. DNA dam-age showed a negative association with AOA and GSH concentrations in the in-fected goats. Conclusion: The Babesia infection increases oxidative stress markers and DNA damage and decreases AOA in goats. These results suggest that the increases in the production of free radicals due to Babesia infection not only contribute to host-de-fense strategies of organisms to kill the parasite but also induce oxidative damage in other cells

The subchronic exposure to malathion, an organophosphate pesticide, causes lipid peroxidation, oxidative stress, and tissue damage in rats: the protective rote of resveratrol

WOS: 000431958400017PubMed ID: 30090600The present study was planned to evaluate the protective role of resveratrol (Res) against subchronic malathion exposure in rats over four weeks. In total, 48 Wistar rats were used and divided equally into six groups. The groups were designed as the control group (received only a rodent diet and tap water), the corn oil group (0.5 ml corn oil by the oral route), and the malathion group (100 mg kg( -1) day by the oral route). Other three groups received malathion (100 mg kg(-1) day(-1)) plus Res (5, 10, and 20 mg kg (-1) day(-1), respectively) by the oral route. Malathion increased malondialdehyde and 8-OHdG levels, whereas it decreased glutathione levels. Also, acetylcholinesterase, superoxide dismutase, and catalase activities were found to be low in the blood, liver, kidney, heart, and brain tissues. Biochemical parameters were not notably changed in all groups. In contrast, Res treatment inverted malathion-induced oxidative stress, lipid peroxidation, and activity of enzymes. Additionally, malathion-induced histopathological changes in the liver, kidney, heart, and brain were ameliorated by Res treatment. These results demonstrate that malathion increases oxidative stress and decreases the antioxidant status while Res has a protective function against malathion toxicity in rats.Technics, Skopje, Macedonia; Uak, Turkey [BAPK-2012/MF003]; Afyon Kocatepe UniversityAfyon Kocatepe University; Usak University Scientific Research Council, Usak, TurkeyUsak University [BAPK-2012/MF003]EA is grateful to Usak University Scientific Research Council, Usak, Turkey for providing financial support (Project no: BAPK-2012/MF003). IK thanks Afyon Kocatepe University, Faculty of Veterinary Medicine, Department of Biochemistry for providing laboratory facilities. Also, this study was orally presented at the 2nd International Congress on Advances in Veterinary Sciences and Technics, Skopje, Macedonia

Increased DNA damage and oxidative stress in chickens with Natural Marek's Disease

Oxidative stress contributes to the accumulation of genomic abnormalities, prevents cellular apoptosis, and also mediates immunosuppression resulting in tumor formation. Marek's Disease provides excellent opportunities for the study of herpesvirus-incluced tumors both in experimental- and natural conditions. The aim of this study was to examine the effects of Marek's Disease (MD) on basal levels of DNA strand breaks and on the oxidative-anti oxidative status of chickens with MD. White-Lohmann hens-fifteen infected with Marek's Disease Virus (MDV) and fifteen healthy-of same age and sex were included in this study. MID infection was diagnosed via clinical signs, gross- and micro-pathological findings and also by detection of viral antigens in feather follicle epithelium by the indirect immunoperoxidase method. Compared with healthy controls, DNA damage was greater and levels of malondialdehyde (MDA) and plasma protein carbonyl (PCO), and plasma concentration of nitric oxide metabolites (NOx) higher in the MD group. Furthermore, total antioxidant activities (AOAs) were found lowered and glutathione (GSH) levels reduced in the MD group compared to the control group. Significantly positive correlation was found between DNA damage, MDA, PCO, and NOx in the MID group. DNA strand breaks were found negatively associated with AOA and GSH concentrations in the MD group. Our results demonstrated that oxidative stress markers and DNA damage substantially increased in chickens with MD, which indicated that increased DNA damage levels might be related to the increased oxidative stress and reduced antioxidant activity. (C) 2009 Elsevier B.V. All rights reserved

Determination of the regulatory properties of Yucca schidigera extracts on the biochemical parameters and plasma hormone levels associated with obesity

ABSTRACT Yucca schidigera Ortgies, Asparagaceae, is a herbaceous plant. Due to the high saponin content the powdered branches and leaves are used as natural food additive for human and animal. The aim of this study was to investigate the effects of Y. schidigera extracts on plasma leptin, ghrelin, adiponectin, insulin, thyroid hormones and some biochemical parameters in mice fed a high-fat diet. Male Swiss Albino mice were divided into seven equal groups. Group I (negative control group) was given standard diet; Group II was given high-fat diet; Group III was given high-fat diet with carboxymethylcellulose; Groups IV–VII were given hexane, petroleum ether, ethyl acetate, and methanol extracts of Y. schidigera and high-fat diet via gastric gavage for 60 days. High-fat diet significantly increased plasma leptin, insulin, free T3 hormone, glucose, cholesterol, low-density lipoprotein, triacylglyceride, aspartate aminotransferase and alanine aminotransferase levels, and significantly decreased plasma ghrelin, adiponectin and free T4 hormone levels. On the other hand, hormone levels, lipid profile and biochemical parameters were improved by the administration of the PE extract. Y. schidigera extracts could be used as preventive medicine in nutritional disorders via regulating energy metabolism and hormonal functions